(Linear) (Six-base) MAPSORT of: KIAA1540.seq  Check: 9170  from: 1  to: 1269



KIAA1540.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:17  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1021    1269 
   Size:       1021     248
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1038    1125    1269 
   Size:       1038      87     144

  Fragments arranged by size: 

                1038     144      87
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     857    1269 
   Size:        857     412
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1013    1269 
   Size:       1013     256
 
BanII G_rGCy'C

Cuts at:      0      56    1182    1269 
   Size:         56    1126      87

  Fragments arranged by size: 

                1126      87      56
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     240     871    1234    1269 
   Size:        240     631     363      35

  Fragments arranged by size: 

                 631     363     240      35
 
BclI T'GATC_A

Cuts at:      0     356    1269 
   Size:        356     913
 
Bme1580I G_kGCm'C

Cuts at:      0     107    1269 
   Size:        107    1162
 
BmgBI CAC'GTC

Cuts at:      0     468    1269 
   Size:        468     801
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     444    1123    1269 
   Size:        444     679     146

  Fragments arranged by size: 

                 679     444     146
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1201    1269 
   Size:       1201      68
 
Bpu10I CC'TnA_GC

Cuts at:      0     686    1269 
   Size:        686     583
 
BsaBI GATnn'nnATC

Cuts at:      0     544    1269 
   Size:        544     725
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     130     160    1269 
   Size:        130      30    1109

  Fragments arranged by size: 

                1109     130      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      19     745     928    1269 
   Size:         19     726     183     341

  Fragments arranged by size: 

                 726     341     183      19
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     448    1269 
   Size:        448     821
 
BsmI GAATG_Cn'

Cuts at:      0     308    1269 
   Size:        308     961
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     474    1269 
   Size:        474     795
 
Bsp1286I G_dGCh'C

Cuts at:      0      56     107    1182    1269 
   Size:         56      51    1075      87

  Fragments arranged by size: 

                1075      87      56      51
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1021    1269 
   Size:       1021     248
 
BsrDI GCAATG_nn'

Cuts at:      0     491    1269 
   Size:        491     778
 
BssSI C'ACGA_G

Cuts at:      0      58     779    1269 
   Size:         58     721     490

  Fragments arranged by size: 

                 721     490      58
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1013    1269 
   Size:       1013     256
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     498    1269 
   Size:        498     771
 
BstYI r'GATC_y

Cuts at:      0     539    1269 
   Size:        539     730
 
Bsu36I CC'TnA_GG

Cuts at:      0    1106    1269 
   Size:       1106     163
 
BtgI C'CryG_G

Cuts at:      0     203     584    1269 
   Size:        203     381     685

  Fragments arranged by size: 

                 685     381     203
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     552     833    1269 
   Size:        552     281     436

  Fragments arranged by size: 

                 552     436     281
 
BtsI GCAGTG_nn'

Cuts at:      0     460    1269 
   Size:        460     809
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1236    1269 
   Size:       1236      33
 
EaeI y'GGCC_r

Cuts at:      0      98     311     722     785    1170    1269 
   Size:         98     213     411      63     385      99

  Fragments arranged by size: 

                 411     385     213      99      98      63
 
EarI CTCTTCn'nnn_

Cuts at:      0     328     706    1269 
   Size:        328     378     563

  Fragments arranged by size: 

                 563     378     328
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1038    1125    1201    1269 
   Size:       1038      87      76      68

  Fragments arranged by size: 

                1038      87      76      68
 
HaeII r_GCGC'y

Cuts at:      0     180     188    1269 
   Size:        180       8    1081

  Fragments arranged by size: 

                1081     180       8
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1071    1103    1225    1257    1269 
   Size:       1071      32     122      32      12

  Fragments arranged by size: 

                1071     122      32      32      12
 
HincII GTy'rAC

Cuts at:      0     333    1269 
   Size:        333     936
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     173    1269 
   Size:        173    1096
 
MscI TGG'CCA

Cuts at:      0     724     787    1172    1269 
   Size:        724      63     385      97

  Fragments arranged by size: 

                 724     385      97      63
 
MslI CAynn'nnrTG

Cuts at:      0     496     766     874    1269 
   Size:        496     270     108     395

  Fragments arranged by size: 

                 496     395     270     108
 
MspA1I CmG'CkG

Cuts at:      0     694    1269 
   Size:        694     575
 
NcoI C'CATG_G

Cuts at:      0     584    1269 
   Size:        584     685
 
NspI r_CATG'y

Cuts at:      0     429     613    1086    1269 
   Size:        429     184     473     183

  Fragments arranged by size: 

                 473     429     184     183
 
PvuII CAG'CTG

Cuts at:      0     694    1269 
   Size:        694     575
 
ScaI AGT'ACT

Cuts at:      0     454     838    1269 
   Size:        454     384     431

  Fragments arranged by size: 

                 454     431     384
 
SexAI A'CCwGG_T

Cuts at:      0     509    1269 
   Size:        509     760
 
StyI C'CwwG_G

Cuts at:      0      35     584    1269 
   Size:         35     549     685

  Fragments arranged by size: 

                 685     549      35
 
TatI w'GTAC_w

Cuts at:      0     452     836    1269 
   Size:        452     384     433

  Fragments arranged by size: 

                 452     433     384
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1219    1269 
   Size:       1219      50
 

 Enzymes that do cut:

     AarI     AcuI     AlfI    AlwNI    BanII     BbsI     BclI Bme1580I
    BmgBI     BmrI     BpmI   Bpu10I    BsaBI    BsaXI    BseRI     BsgI
     BsmI    BsmBI Bsp1286I    BspMI    BsrDI    BssSI   BstAPI    BstXI
    BstYI   Bsu36I     BtgI    BtgZI     BtsI     DrdI     EaeI     EarI
  Eco57MI    HaeII    Hin4I   HincII     MmeI     MscI     MslI   MspA1I
     NcoI     NspI    PvuII     ScaI    SexAI     StyI     TatI     XcmI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AloI     ApaI    ApaLI     ApoI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI     BbeI    BbvCI
     BcgI    BciVI    BfrBI     BglI    BglII     BlpI     BmtI     BplI
    BpuEI     BsaI    BsaAI    BsaHI    BsaWI    BseYI    BsiEI  BsiHKAI
    BsiWI    BspEI    BspHI    BsrBI    BsrFI    BsrGI   BssHII    BstBI
   BstEII  BstZ17I     ClaI    CspCI     DraI   DraIII     EagI     EciI
  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI  HindIII     HpaI     KasI     KpnI     MfeI     MluI     NaeI
     NarI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI
     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     SfcI     SfiI
     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     SwaI    TaqII     TstI  Tth111I     XbaI
     XhoI     XmaI     XmnI     ZraI