Restriction map for
pF1KA1549
(Linear) (Six-base) MAPSORT of: KIAA1549.seq Check: 2546 from: 1 to: 5574
KIAA1549.orf
With 182 enzymes: SgfI *
October 24, 2006 15:08 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1713 3429 4885 5574
Size: 1713 1716 1456 689
Fragments arranged by size:
1716 1713 1456 689
AccI GT'mk_AC
Cuts at: 0 1969 2570 3623 4421 5095 5574
Size: 1969 601 1053 798 674 479
Fragments arranged by size:
1969 1053 798 674 601 479
Acc65I G'GTAC_C
Cuts at: 0 5215 5574
Size: 5215 359
AclI AA'CG_TT
Cuts at: 0 1593 4560 5574
Size: 1593 2967 1014
Fragments arranged by size:
2967 1593 1014
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 34 1544 2348 2755 2905 3289 3934 5366
Size: 34 1510 804 407 150 384 645 1432
Cuts at: 5366 5574
Size: 208
Fragments arranged by size:
1510 1432 804 645 407 384 208 150
34
AfeI AGC'GCT
Cuts at: 0 36 5574
Size: 36 5538
AflIII A'CryG_T
Cuts at: 0 197 2234 2294 5574
Size: 197 2037 60 3280
Fragments arranged by size:
3280 2037 197 60
AgeI A'CCGG_T
Cuts at: 0 2434 5574
Size: 2434 3140
AhdI GACnn_n'nnGTC
Cuts at: 0 1240 4428 5574
Size: 1240 3188 1146
Fragments arranged by size:
3188 1240 1146
AleI CACnn'nnGTG
Cuts at: 0 742 2392 2416 2647 5574
Size: 742 1650 24 231 2927
Fragments arranged by size:
2927 1650 742 231 24
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 53 583 1150 5574
Size: 53 530 567 4424
Fragments arranged by size:
4424 567 530 53
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 4545 4577 5574
Size: 4545 32 997
Fragments arranged by size:
4545 997 32
AlwNI CAG_nnn'CTG
Cuts at: 0 61 2071 3267 3804 3849 4018 5241 5574
Size: 61 2010 1196 537 45 169 1223 333
Fragments arranged by size:
2010 1223 1196 537 333 169 61 45
ApaI G_GGCC'C
Cuts at: 0 2374 3097 5440 5574
Size: 2374 723 2343 134
Fragments arranged by size:
2374 2343 723 134
ApoI r'AATT_y
Cuts at: 0 516 1189 3596 5574
Size: 516 673 2407 1978
Fragments arranged by size:
2407 1978 673 516
AvaI C'yCGr_G
Cuts at: 0 1527 1951 4434 5574
Size: 1527 424 2483 1140
Fragments arranged by size:
2483 1527 1140 424
BamHI G'GATC_C
Cuts at: 0 2311 4704 5574
Size: 2311 2393 870
Fragments arranged by size:
2393 2311 870
BanI G'GyrC_C
Cuts at: 0 1111 2079 2394 4257 4648 5106 5215 5574
Size: 1111 968 315 1863 391 458 109 359
Fragments arranged by size:
1863 1111 968 458 391 359 315 109
BanII G_rGCy'C
Cuts at: 0 8 1003 2374 2497 3097 3233 4107 4538
Size: 8 995 1371 123 600 136 874 431
Cuts at: 4538 4549 5277 5396 5440 5528 5574
Size: 11 728 119 44 88 46
Fragments arranged by size:
1371 995 874 728 600 431 136 123
119 88 46 44 11 8
BbsI GAAGACnn'nnnn_
Cuts at: 0 1562 2033 2168 3530 5033 5477 5574
Size: 1562 471 135 1362 1503 444 97
Fragments arranged by size:
1562 1503 1362 471 444 135 97
BbvCI CC'TCA_GC
Cuts at: 0 1058 2007 5120 5397 5574
Size: 1058 949 3113 277 177
Fragments arranged by size:
3113 1058 949 277 177
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1427 1461 4837 4871 5574
Size: 1427 34 3376 34 703
Fragments arranged by size:
3376 1427 703 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 2937 3199 3251 4769 5574
Size: 2937 262 52 1518 805
Fragments arranged by size:
2937 1518 805 262 52
BclI T'GATC_A
Cuts at: 0 2285 2639 5574
Size: 2285 354 2935
Fragments arranged by size:
2935 2285 354
BglI GCCn_nnn'nGGC
Cuts at: 0 4864 4999 5328 5574
Size: 4864 135 329 246
Fragments arranged by size:
4864 329 246 135
BglII A'GATC_T
Cuts at: 0 3047 5574
Size: 3047 2527
BlpI GC'TnA_GC
Cuts at: 0 3370 3839 5276 5574
Size: 3370 469 1437 298
Fragments arranged by size:
3370 1437 469 298
Bme1580I G_kGCm'C
Cuts at: 0 32 2084 2374 3097 4403 4476 4520 5111
Size: 32 2052 290 723 1306 73 44 591
Cuts at: 5111 5440 5574
Size: 329 134
Fragments arranged by size:
2052 1306 723 591 329 290 134 73
44 32
BmgBI CAC'GTC
Cuts at: 0 1095 3948 5574
Size: 1095 2853 1626
Fragments arranged by size:
2853 1626 1095
BmrI ACTGGGnnnn_n'
Cuts at: 0 35 1823 3205 3688 4124 4214 4759 4779
Size: 35 1788 1382 483 436 90 545 20
Cuts at: 4779 4962 5574
Size: 183 612
Fragments arranged by size:
1788 1382 612 545 483 436 183 90
35 20
BmtI G_CTAG'C
Cuts at: 0 4208 5574
Size: 4208 1366
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 3538 3659 4167 5574
Size: 3538 121 508 1407
Fragments arranged by size:
3538 1407 508 121
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 686 915 1215 1843 3304 3481 3550 5461
Size: 686 229 300 628 1461 177 69 1911
Cuts at: 5461 5574
Size: 113
Fragments arranged by size:
1911 1461 686 628 300 229 177 113
69
Bpu10I CC'TnA_GC
Cuts at: 0 997 1058 2007 5120 5397 5574
Size: 997 61 949 3113 277 177
Fragments arranged by size:
3113 997 949 277 177 61
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1190 1864 2056 3057 3195 5574
Size: 1190 674 192 1001 138 2379
Fragments arranged by size:
2379 1190 1001 674 192 138
BsaI GGTCTCn'nnnn_
Cuts at: 0 696 1517 5574
Size: 696 821 4057
Fragments arranged by size:
4057 821 696
BsaAI yAC'GTr
Cuts at: 0 198 2235 4176 5574
Size: 198 2037 1941 1398
Fragments arranged by size:
2037 1941 1398 198
BsaBI GATnn'nnATC
Cuts at: 0 2633 3473 4483 4698 5574
Size: 2633 840 1010 215 876
Fragments arranged by size:
2633 1010 876 840 215
BsaHI Gr'CG_yC
Cuts at: 0 1366 1417 2480 3302 4069 4670 4850 5574
Size: 1366 51 1063 822 767 601 180 724
Fragments arranged by size:
1366 1063 822 767 724 601 180 51
BsaWI w'CCGG_w
Cuts at: 0 2434 2779 3477 3645 5574
Size: 2434 345 698 168 1929
Fragments arranged by size:
2434 1929 698 345 168
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 768 798 831 861 4171 4201 5170 5200
Size: 768 30 33 30 3310 30 969 30
Cuts at: 5200 5574
Size: 374
Fragments arranged by size:
3310 969 768 374 33 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 721 854 2507 3074 4828 5191 5481 5518
Size: 721 133 1653 567 1754 363 290 37
Cuts at: 5518 5536 5574
Size: 18 38
Fragments arranged by size:
1754 1653 721 567 363 290 133 38
37 18
BseYI C'CCAG_C
Cuts at: 0 74 175 2966 3362 3614 4281 4716 4799
Size: 74 101 2791 396 252 667 435 83
Cuts at: 4799 4870 4989 5445 5574
Size: 71 119 456 129
Fragments arranged by size:
2791 667 456 435 396 252 129 119
101 83 74 71
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 23 746 1026 2057 2689 2744 3064 3457
Size: 23 723 280 1031 632 55 320 393
Cuts at: 3457 3548 4747 5574
Size: 91 1199 827
Fragments arranged by size:
1199 1031 827 723 632 393 320 280
91 55 23
BsiEI CG_ry'CG
Cuts at: 0 1350 4290 5574
Size: 1350 2940 1284
Fragments arranged by size:
2940 1350 1284
BsiHKAI G_wGCw'C
Cuts at: 0 8 2223 2425 4107 4175 4361 4549 5277
Size: 8 2215 202 1682 68 186 188 728
Cuts at: 5277 5528 5554 5574
Size: 251 26 20
Fragments arranged by size:
2215 1682 728 251 202 188 186 68
26 20 8
BsiWI C'GTAC_G
Cuts at: 0 5336 5574
Size: 5336 238
BsmI GAATG_Cn'
Cuts at: 0 829 1460 5574
Size: 829 631 4114
Fragments arranged by size:
4114 829 631
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1270 1715 3647 3938 5574
Size: 1270 445 1932 291 1636
Fragments arranged by size:
1932 1636 1270 445 291
Bsp1286I G_dGCh'C
Cuts at: 0 8 32 1003 2084 2223 2374 2425 2497
Size: 8 24 971 1081 139 151 51 72
Cuts at: 2497 3097 3233 4107 4175 4361 4403 4476 4520
Size: 600 136 874 68 186 42 73 44
Cuts at: 4520 4538 4549 5111 5277 5396 5440 5528 5554
Size: 18 11 562 166 119 44 88 26
Cuts at: 5554 5574
Size: 20
Fragments arranged by size:
1081 971 874 600 562 186 166 151
139 136 119 88 73 72 68 51
44 44 42 26 24 20 18 11
8
BspEI T'CCGG_A
Cuts at: 0 3645 5574
Size: 3645 1929
BspHI T'CATG_A
Cuts at: 0 3885 5574
Size: 3885 1689
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1713 2309 3429 4082 4885 5574
Size: 1713 596 1120 653 803 689
Fragments arranged by size:
1713 1120 803 689 653 596
BsrBI CCG'CTC
Cuts at: 0 4115 5574
Size: 4115 1459
BsrDI GCAATG_nn'
Cuts at: 0 850 3608 5056 5574
Size: 850 2758 1448 518
Fragments arranged by size:
2758 1448 850 518
BsrFI r'CCGG_y
Cuts at: 0 1362 2434 4208 4622 5574
Size: 1362 1072 1774 414 952
Fragments arranged by size:
1774 1362 1072 952 414
BssHII G'CGCG_C
Cuts at: 0 4383 5574
Size: 4383 1191
BssSI C'ACGA_G
Cuts at: 0 7 1074 4861 5574
Size: 7 1067 3787 713
Fragments arranged by size:
3787 1067 713 7
BstAPI GCAn_nnn'nTGC
Cuts at: 0 826 2071 5574
Size: 826 1245 3503
Fragments arranged by size:
3503 1245 826
BstEII G'GTnAC_C
Cuts at: 0 3589 5449 5574
Size: 3589 1860 125
Fragments arranged by size:
3589 1860 125
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1028 2472 3585 5574
Size: 1028 1444 1113 1989
Fragments arranged by size:
1989 1444 1113 1028
BstYI r'GATC_y
Cuts at: 0 171 1200 1258 2311 3047 3809 3968 4184
Size: 171 1029 58 1053 736 762 159 216
Cuts at: 4184 4226 4373 4493 4704 5165 5231 5574
Size: 42 147 120 211 461 66 343
Fragments arranged by size:
1053 1029 762 736 461 343 216 211
171 159 147 120 66 58 42
BtgI C'CryG_G
Cuts at: 0 277 368 1262 1344 1924 2411 4395 4505
Size: 277 91 894 82 580 487 1984 110
Cuts at: 4505 5465 5574
Size: 960 109
Fragments arranged by size:
1984 960 894 580 487 277 110 109
91 82
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 797 4348 4658 5574
Size: 797 3551 310 916
Fragments arranged by size:
3551 916 797 310
BtsI GCAGTG_nn'
Cuts at: 0 1433 2069 2732 3898 4123 5574
Size: 1433 636 663 1166 225 1451
Fragments arranged by size:
1451 1433 1166 663 636 225
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 105 140 1824 1859 5574
Size: 105 35 1684 35 3715
Fragments arranged by size:
3715 1684 105 35 35
DraI TTT'AAA
Cuts at: 0 1883 5574
Size: 1883 3691
DraIII CAC_nnn'GTG
Cuts at: 0 1031 4461 4970 5574
Size: 1031 3430 509 604
Fragments arranged by size:
3430 1031 604 509
DrdI GACnn_nn'nnGTC
Cuts at: 0 4027 5574
Size: 4027 1547
EaeI y'GGCC_r
Cuts at: 0 221 1070 1347 2654 3779 4508 5000 5484
Size: 221 849 277 1307 1125 729 492 484
Cuts at: 5484 5574
Size: 90
Fragments arranged by size:
1307 1125 849 729 492 484 277 221
90
EagI C'GGCC_G
Cuts at: 0 1347 5574
Size: 1347 4227
EarI CTCTTCn'nnn_
Cuts at: 0 1692 1829 2655 3628 3993 4031 4537 4946
Size: 1692 137 826 973 365 38 506 409
Cuts at: 4946 5355 5574
Size: 409 219
Fragments arranged by size:
1692 973 826 506 409 409 365 219
137 38
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 435 3408 4312 4366 5127 5574
Size: 435 2973 904 54 761 447
Fragments arranged by size:
2973 904 761 447 435 54
EcoICRI GAG'CTC
Cuts at: 0 6 4105 4547 5275 5526 5574
Size: 6 4099 442 728 251 48
Fragments arranged by size:
4099 728 442 251 48 6
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 34 686 915 1215 1544 1843 2348 2755
Size: 34 652 229 300 329 299 505 407
Cuts at: 2755 2905 3289 3304 3481 3550 3934 5366 5461
Size: 150 384 15 177 69 384 1432 95
Cuts at: 5461 5574
Size: 113
Fragments arranged by size:
1432 652 505 407 384 384 329 300
299 229 177 150 113 95 69 34
15
EcoNI CCTnn'n_nnAGG
Cuts at: 0 592 4656 5245 5574
Size: 592 4064 589 329
Fragments arranged by size:
4064 592 589 329
EcoO109I rG'GnC_Cy
Cuts at: 0 3093 3907 4094 4215 4661 4903 4976 5436
Size: 3093 814 187 121 446 242 73 460
Cuts at: 5436 5437 5574
Size: 1 137
Fragments arranged by size:
3093 814 460 446 242 187 137 121
73 1
EcoRI G'AATT_C
Cuts at: 0 3596 5574
Size: 3596 1978
EcoRV GAT'ATC
Cuts at: 0 2616 5574
Size: 2616 2958
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1955 3777 5574
Size: 1955 1822 1797
Fragments arranged by size:
1955 1822 1797
HaeII r_GCGC'y
Cuts at: 0 38 3898 4275 4395 5574
Size: 38 3860 377 120 1179
Fragments arranged by size:
3860 1179 377 120 38
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 273 305 831 863 889 921 1084 1116
Size: 273 32 526 32 26 32 163 32
Cuts at: 1116 1226 1258 1624 1656 1792 1824 3563 3595
Size: 110 32 366 32 136 32 1739 32
Cuts at: 3595 4006 4038 4078 4110 4171 4203 4414 4417
Size: 411 32 40 32 61 32 211 3
Cuts at: 4417 4446 4449 5170 5202 5574
Size: 29 3 721 32 372
Fragments arranged by size:
1739 721 526 411 372 366 273 211
163 136 110 61 40 32 32 32
32 32 32 32 32 32 32 32
32 29 26 3 3
HincII GTy'rAC
Cuts at: 0 2571 3624 4083 4593 5574
Size: 2571 1053 459 510 981
Fragments arranged by size:
2571 1053 981 510 459
HindIII A'AGCT_T
Cuts at: 0 1932 2833 5574
Size: 1932 901 2741
Fragments arranged by size:
2741 1932 901
KpnI G_GTAC'C
Cuts at: 0 5219 5574
Size: 5219 355
MluI A'CGCG_T
Cuts at: 0 2294 5574
Size: 2294 3280
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 526 3128 5574
Size: 526 2602 2446
Fragments arranged by size:
2602 2446 526
MscI TGG'CCA
Cuts at: 0 1072 2656 3781 5486 5574
Size: 1072 1584 1125 1705 88
Fragments arranged by size:
1705 1584 1125 1072 88
MslI CAynn'nnrTG
Cuts at: 0 184 742 1132 1891 2392 2416 2470 2647
Size: 184 558 390 759 501 24 54 177
Cuts at: 2647 2815 3694 5574
Size: 168 879 1880
Fragments arranged by size:
1880 879 759 558 501 390 184 177
168 54 24
MspA1I CmG'CkG
Cuts at: 0 430 1665 1676 1818 3366 4397 5574
Size: 430 1235 11 142 1548 1031 1177
Fragments arranged by size:
1548 1235 1177 1031 430 142 11
NaeI GCC'GGC
Cuts at: 0 1364 4210 5574
Size: 1364 2846 1364
Fragments arranged by size:
2846 1364 1364
NcoI C'CATG_G
Cuts at: 0 368 1262 1924 5574
Size: 368 894 662 3650
Fragments arranged by size:
3650 894 662 368
NdeI CA'TA_TG
Cuts at: 0 435 2604 5574
Size: 435 2169 2970
Fragments arranged by size:
2970 2169 435
NgoMIV G'CCGG_C
Cuts at: 0 1362 4208 5574
Size: 1362 2846 1366
Fragments arranged by size:
2846 1366 1362
NheI G'CTAG_C
Cuts at: 0 4204 5574
Size: 4204 1370
PasI CC'CwG_GG
Cuts at: 0 329 5440 5574
Size: 329 5111 134
Fragments arranged by size:
5111 329 134
PflMI CCAn_nnn'nTGG
Cuts at: 0 508 4970 5074 5574
Size: 508 4462 104 500
Fragments arranged by size:
4462 508 500 104
PfoI T'CCnGG_A
Cuts at: 0 4496 4841 5407 5574
Size: 4496 345 566 167
Fragments arranged by size:
4496 566 345 167
PmlI CAC'GTG
Cuts at: 0 2235 4176 5574
Size: 2235 1941 1398
Fragments arranged by size:
2235 1941 1398
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 335 367 2884 2916 5574
Size: 335 32 2517 32 2658
Fragments arranged by size:
2658 2517 335 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 3907 4094 4215 4976 5574
Size: 3907 187 121 761 598
Fragments arranged by size:
3907 761 598 187 121
PshAI GACnn'nnGTC
Cuts at: 0 2146 5574
Size: 2146 3428
PsiI TTA'TAA
Cuts at: 0 2824 5574
Size: 2824 2750
PspOMI G'GGCC_C
Cuts at: 0 2370 3093 5436 5574
Size: 2370 723 2343 138
Fragments arranged by size:
2370 2343 723 138
PstI C_TGCA'G
Cuts at: 0 1808 3221 3423 3809 5017 5093 5435 5574
Size: 1808 1413 202 386 1208 76 342 139
Fragments arranged by size:
1808 1413 1208 386 342 202 139 76
PvuII CAG'CTG
Cuts at: 0 1818 3366 5574
Size: 1818 1548 2208
Fragments arranged by size:
2208 1818 1548
SacI G_AGCT'C
Cuts at: 0 8 4107 4549 5277 5528 5574
Size: 8 4099 442 728 251 46
Fragments arranged by size:
4099 728 442 251 46 8
SacII CC_GC'GG
Cuts at: 0 4398 5574
Size: 4398 1176
SalI G'TCGA_C
Cuts at: 0 2569 3622 5574
Size: 2569 1053 1952
Fragments arranged by size:
2569 1952 1053
SanDI GG'GwC_CC
Cuts at: 0 4215 5574
Size: 4215 1359
SapI GCTCTTCn'nnn_
Cuts at: 0 2655 4537 5574
Size: 2655 1882 1037
Fragments arranged by size:
2655 1882 1037
ScaI AGT'ACT
Cuts at: 0 2702 2918 5574
Size: 2702 216 2656
Fragments arranged by size:
2702 2656 216
SexAI A'CCwGG_T
Cuts at: 0 4586 5067 5574
Size: 4586 481 507
Fragments arranged by size:
4586 507 481
SfcI C'TryA_G
Cuts at: 0 1804 3217 3413 3419 3805 4653 4983 5013
Size: 1804 1413 196 6 386 848 330 30
Cuts at: 5013 5089 5096 5431 5574
Size: 76 7 335 143
Fragments arranged by size:
1804 1413 848 386 335 330 196 143
76 30 7 6
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 5328 5574
Size: 5328 246
SgrAI Cr'CCGG_yG
Cuts at: 0 2434 5574
Size: 2434 3140
SmlI C'TyrA_G
Cuts at: 0 1205 1843 1951 2035 3072 3174 5574
Size: 1205 638 108 84 1037 102 2400
Fragments arranged by size:
2400 1205 1037 638 108 102 84
StuI AGG'CCT
Cuts at: 0 2984 5134 5574
Size: 2984 2150 440
Fragments arranged by size:
2984 2150 440
StyI C'CwwG_G
Cuts at: 0 144 368 680 1262 1766 1924 5374 5574
Size: 144 224 312 582 504 158 3450 200
Fragments arranged by size:
3450 582 504 312 224 200 158 144
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 458 1437 2400 2464 2980 4304 4981 5574
Size: 458 979 963 64 516 1324 677 593
Fragments arranged by size:
1324 979 963 677 593 516 458 64
TatI w'GTAC_w
Cuts at: 0 163 2675 2700 2916 4787 5574
Size: 163 2512 25 216 1871 787
Fragments arranged by size:
2512 1871 787 216 163 25
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1317 1349 2436 2468 3197 3229 4250 4282
Size: 1317 32 1087 32 729 32 1021 32
Cuts at: 4282 5574
Size: 1292
Fragments arranged by size:
1317 1292 1087 1021 729 32 32 32
32
XbaI T'CTAG_A
Cuts at: 0 1255 2223 5574
Size: 1255 968 3351
Fragments arranged by size:
3351 1255 968
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 183 263 279 710 1080 5574
Size: 183 80 16 431 370 4494
Fragments arranged by size:
4494 431 370 183 80 16
XhoI C'TCGA_G
Cuts at: 0 1951 5574
Size: 1951 3623
XmnI GAAnn'nnTTC
Cuts at: 0 3332 3826 4002 5574
Size: 3332 494 176 1572
Fragments arranged by size:
3332 1572 494 176
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApoI AvaI
BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssHII BssSI BstAPI BstEII BstXI BstYI BtgI BtgZI
BtsI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
HaeII Hin4I HincII HindIII KpnI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI PasI
PflMI PfoI PmlI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuII SacI SacII SalI SanDI SapI ScaI
SexAI SfcI SfiI SgrAI SmlI StuI StyI TaqII
TatI TstI XbaI XcmI XhoI XmnI
Enzymes that do not cut:
AatII AflII ApaLI AscI AseI AsiSI AvrII BaeI
BbeI BfrBI BsrGI BstBI BstZ17I Bsu36I ClaI FseI
FspI FspAI HpaI KasI MfeI NarI NotI NruI
NsiI NspI PacI PciI PmeI PspXI PsrI PvuI
RsrII SbfI SfoI SgfI SmaI SnaBI SpeI SphI
SrfI SspI SwaI Tth111I XmaI ZraI