Restriction map for
pF1KSDA1552
(Linear) (Six-base) MAPSORT of: KIAA1552.seq Check: 8416 from: 1 to: 2145
KIAA1552.orf
With 182 enzymes: SgfI *
October 24, 2006 15:17 ..
AatII G_ACGT'C
Cuts at: 0 83 2123 2145
Size: 83 2040 22
Fragments arranged by size:
2040 83 22
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1282 1546 2145
Size: 1282 264 599
Fragments arranged by size:
1282 599 264
AfeI AGC'GCT
Cuts at: 0 775 2145
Size: 775 1370
AflIII A'CryG_T
Cuts at: 0 2077 2145
Size: 2077 68
AlwNI CAG_nnn'CTG
Cuts at: 0 246 336 1191 1691 1964 2145
Size: 246 90 855 500 273 181
Fragments arranged by size:
855 500 273 246 181 90
ApaI G_GGCC'C
Cuts at: 0 619 658 2145
Size: 619 39 1487
Fragments arranged by size:
1487 619 39
ApaLI G'TGCA_C
Cuts at: 0 184 1828 2145
Size: 184 1644 317
Fragments arranged by size:
1644 317 184
AvaI C'yCGr_G
Cuts at: 0 10 479 679 737 808 1204 1242 1516
Size: 10 469 200 58 71 396 38 274
Cuts at: 1516 2145
Size: 629
Fragments arranged by size:
629 469 396 274 200 71 58 38
10
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 838 871 2145
Size: 838 33 1274
Fragments arranged by size:
1274 838 33
BamHI G'GATC_C
Cuts at: 0 166 2145
Size: 166 1979
BanI G'GyrC_C
Cuts at: 0 548 732 2145
Size: 548 184 1413
Fragments arranged by size:
1413 548 184
BanII G_rGCy'C
Cuts at: 0 17 602 619 641 658 680 686 744
Size: 17 585 17 22 17 22 6 58
Cuts at: 744 1507 2145
Size: 763 638
Fragments arranged by size:
763 638 585 58 22 22 17 17
17 6
BbeI G_GCGC'C
Cuts at: 0 552 2145
Size: 552 1593
BbsI GAAGACnn'nnnn_
Cuts at: 0 1187 1270 1534 2145
Size: 1187 83 264 611
Fragments arranged by size:
1187 611 264 83
BglI GCCn_nnn'nGGC
Cuts at: 0 623 1511 2145
Size: 623 888 634
Fragments arranged by size:
888 634 623
BlpI GC'TnA_GC
Cuts at: 0 449 703 754 2145
Size: 449 254 51 1391
Fragments arranged by size:
1391 449 254 51
Bme1580I G_kGCm'C
Cuts at: 0 188 619 658 670 983 1832 2145
Size: 188 431 39 12 313 849 313
Fragments arranged by size:
849 431 313 313 188 39 12
BmgBI CAC'GTC
Cuts at: 0 824 2078 2145
Size: 824 1254 67
Fragments arranged by size:
1254 824 67
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 223 358 400 559 641 664 739 1474
Size: 223 135 42 159 82 23 75 735
Cuts at: 1474 1738 1810 2011 2145
Size: 264 72 201 134
Fragments arranged by size:
735 264 223 201 159 135 134 82
75 72 42 23
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2019 2145
Size: 2019 126
BsaHI Gr'CG_yC
Cuts at: 0 80 549 1267 1531 2120 2145
Size: 80 469 718 264 589 25
Fragments arranged by size:
718 589 469 264 80 25
BsaWI w'CCGG_w
Cuts at: 0 1701 2145
Size: 1701 444
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 257 287 614 644 1324 1354 1588 1618
Size: 257 30 327 30 680 30 234 30
Cuts at: 1618 1861 1891 2067 2097 2145
Size: 243 30 176 30 48
Fragments arranged by size:
680 327 257 243 234 176 48 30
30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 271 649 688 736 739 742 2145
Size: 271 378 39 48 3 3 1403
Fragments arranged by size:
1403 378 271 48 39 3 3
BseYI C'CCAG_C
Cuts at: 0 16 453 568 789 1054 1199 1327 1864
Size: 16 437 115 221 265 145 128 537
Cuts at: 1864 1905 2030 2145
Size: 41 125 115
Fragments arranged by size:
537 437 265 221 145 128 125 115
115 41 16
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 327 907 2145
Size: 327 580 1238
Fragments arranged by size:
1238 580 327
BsiEI CG_ry'CG
Cuts at: 0 1592 2145
Size: 1592 553
BsiHKAI G_wGCw'C
Cuts at: 0 188 1223 1742 1832 2145
Size: 188 1035 519 90 313
Fragments arranged by size:
1035 519 313 188 90
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1036 2145
Size: 1036 1109
Bsp1286I G_dGCh'C
Cuts at: 0 17 188 602 619 641 658 670 680
Size: 17 171 414 17 22 17 12 10
Cuts at: 680 686 744 983 1223 1507 1742 1832 2145
Size: 6 58 239 240 284 235 90 313
Fragments arranged by size:
414 313 284 240 239 235 171 90
58 22 17 17 17 12 10 6
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 913 1357 1621 1808 2145
Size: 913 444 264 187 337
Fragments arranged by size:
913 444 337 264 187
BsrBI CCG'CTC
Cuts at: 0 2013 2145
Size: 2013 132
BsrFI r'CCGG_y
Cuts at: 0 46 711 1310 1574 1664 2145
Size: 46 665 599 264 90 481
Fragments arranged by size:
665 599 481 264 90 46
BsrGI T'GTAC_A
Cuts at: 0 392 2145
Size: 392 1753
BssSI C'ACGA_G
Cuts at: 0 850 1831 2145
Size: 850 981 314
Fragments arranged by size:
981 850 314
BstAPI GCAn_nnn'nTGC
Cuts at: 0 246 1373 1637 1691 1964 2145
Size: 246 1127 264 54 273 181
Fragments arranged by size:
1127 273 264 246 181 54
BstYI r'GATC_y
Cuts at: 0 166 242 1773 2145
Size: 166 76 1531 372
Fragments arranged by size:
1531 372 166 76
Bsu36I CC'TnA_GG
Cuts at: 0 346 611 671 817 1462 1798 2145
Size: 346 265 60 146 645 336 347
Fragments arranged by size:
645 347 346 336 265 146 60
BtgI C'CryG_G
Cuts at: 0 658 1037 2024 2145
Size: 658 379 987 121
Fragments arranged by size:
987 658 379 121
BtsI GCAGTG_nn'
Cuts at: 0 186 416 510 670 748 981 1425 1689
Size: 186 230 94 160 78 233 444 264
Cuts at: 1689 1765 1962 2145
Size: 76 197 183
Fragments arranged by size:
444 264 233 230 197 186 183 160
94 78 76
EaeI y'GGCC_r
Cuts at: 0 48 1589 2145
Size: 48 1541 556
Fragments arranged by size:
1541 556 48
EagI C'GGCC_G
Cuts at: 0 1589 2145
Size: 1589 556
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 223 358 400 559 641 664 739 1282
Size: 223 135 42 159 82 23 75 543
Cuts at: 1282 1474 1546 1738 1810 2011 2145
Size: 192 72 192 72 201 134
Fragments arranged by size:
543 223 201 192 192 159 135 134
82 75 72 72 42 23
EcoNI CCTnn'n_nnAGG
Cuts at: 0 583 721 2145
Size: 583 138 1424
Fragments arranged by size:
1424 583 138
EcoO109I rG'GnC_Cy
Cuts at: 0 607 615 616 1005 1132 1209 1249 1458
Size: 607 8 1 389 127 77 40 209
Cuts at: 1458 1513 1722 2040 2145
Size: 55 209 318 105
Fragments arranged by size:
607 389 318 209 209 127 105 77
55 40 8 1
FseI GG_CCGG'CC
Cuts at: 0 50 2145
Size: 50 2095
FspI TGC'GCA
Cuts at: 0 1684 1957 2145
Size: 1684 273 188
Fragments arranged by size:
1684 273 188
HaeII r_GCGC'y
Cuts at: 0 552 777 2145
Size: 552 225 1368
Fragments arranged by size:
1368 552 225
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 257 289 1160 1192 2067 2099 2122 2145
Size: 257 32 871 32 875 32 23 23
Fragments arranged by size:
875 871 257 32 32 32 23 23
KasI G'GCGC_C
Cuts at: 0 548 2145
Size: 548 1597
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 167 2145
Size: 167 1978
MslI CAynn'nnrTG
Cuts at: 0 499 1678 1951 2145
Size: 499 1179 273 194
Fragments arranged by size:
1179 499 273 194
MspA1I CmG'CkG
Cuts at: 0 960 1470 1497 1734 2064 2145
Size: 960 510 27 237 330 81
Fragments arranged by size:
960 510 330 237 81 27
NaeI GCC'GGC
Cuts at: 0 48 713 1666 2145
Size: 48 665 953 479
Fragments arranged by size:
953 665 479 48
NarI GG'CG_CC
Cuts at: 0 549 2145
Size: 549 1596
NgoMIV G'CCGG_C
Cuts at: 0 46 711 1664 2145
Size: 46 665 953 481
Fragments arranged by size:
953 665 481 46
NspI r_CATG'y
Cuts at: 0 448 2145
Size: 448 1697
PasI CC'CwG_GG
Cuts at: 0 107 603 951 1069 1272 1395 1536 1659
Size: 107 496 348 118 203 123 141 123
Cuts at: 1659 1809 1932 2145
Size: 150 123 213
Fragments arranged by size:
496 348 213 203 150 141 123 123
123 118 107
PflMI CCAn_nnn'nTGG
Cuts at: 0 1230 2145
Size: 1230 915
PpuMI rG'GwC_Cy
Cuts at: 0 607 1132 2040 2145
Size: 607 525 908 105
Fragments arranged by size:
908 607 525 105
PspOMI G'GGCC_C
Cuts at: 0 615 654 2145
Size: 615 39 1491
Fragments arranged by size:
1491 615 39
PspXI vC'TCGA_Gb
Cuts at: 0 808 2145
Size: 808 1337
PstI C_TGCA'G
Cuts at: 0 1052 2145
Size: 1052 1093
PvuII CAG'CTG
Cuts at: 0 2064 2145
Size: 2064 81
SanDI GG'GwC_CC
Cuts at: 0 1132 2145
Size: 1132 1013
SfcI C'TryA_G
Cuts at: 0 1048 1845 2145
Size: 1048 797 300
Fragments arranged by size:
1048 797 300
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 623 2145
Size: 623 1522
SfoI GGC'GCC
Cuts at: 0 550 2145
Size: 550 1595
SmaI CCC'GGG
Cuts at: 0 1244 2145
Size: 1244 901
SmlI C'TyrA_G
Cuts at: 0 808 1998 2145
Size: 808 1190 147
Fragments arranged by size:
1190 808 147
StuI AGG'CCT
Cuts at: 0 485 536 1152 1451 1988 1996 2145
Size: 485 51 616 299 537 8 149
Fragments arranged by size:
616 537 485 299 149 51 8
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 964 1408 1672 1712 2145
Size: 964 444 264 40 433
Fragments arranged by size:
964 444 433 264 40
TatI w'GTAC_w
Cuts at: 0 392 425 1877 2145
Size: 392 33 1452 268
Fragments arranged by size:
1452 392 268 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 614 646 947 979 2145
Size: 614 32 301 32 1166
Fragments arranged by size:
1166 614 301 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 154 693 2101 2145
Size: 154 539 1408 44
Fragments arranged by size:
1408 539 154 44
XhoI C'TCGA_G
Cuts at: 0 808 2145
Size: 808 1337
XmaI C'CCGG_G
Cuts at: 0 1242 2145
Size: 1242 903
XmnI GAAnn'nnTTC
Cuts at: 0 2051 2145
Size: 2051 94
ZraI GAC'GTC
Cuts at: 0 81 2121 2145
Size: 81 2040 24
Fragments arranged by size:
2040 81 24
Enzymes that do cut:
AatII AcuI AfeI AflIII AlwNI ApaI ApaLI AvaI
BaeI BamHI BanI BanII BbeI BbsI BglI BlpI
Bme1580I BmgBI BpmI BpuEI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrBI
BsrFI BsrGI BssSI BstAPI BstYI Bsu36I BtgI BtsI
EaeI EagI Eco57MI EcoNI EcoO109I FseI FspI HaeII
Hin4I KasI MmeI MslI MspA1I NaeI NarI NgoMIV
NspI PasI PflMI PpuMI PspOMI PspXI PstI PvuII
SanDI SfcI SfiI SfoI SmaI SmlI StuI TaqII
TatI TstI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AflII AgeI AhdI AleI
AlfI AloI ApoI AscI AseI AsiSI AvrII BbvCI
BcgI BciVI BclI BfrBI BglII BmrI BmtI BplI
Bpu10I BsaI BsaAI BsaBI BsiWI BsmI BspEI BspHI
BsrDI BssHII BstBI BstEII BstXI BstZ17I BtgZI ClaI
CspCI DraI DraIII DrdI EarI EciI EcoICRI EcoRI
EcoRV FalI FspAI HincII HindIII HpaI KpnI MfeI
MluI MscI NcoI NdeI NheI NotI NruI NsiI
PacI PciI PfoI PmeI PmlI PpiI PshAI PsiI
PsrI PvuI RsrII SacI SacII SalI SapI SbfI
ScaI SexAI SgfI SgrAI SnaBI SpeI SphI SrfI
SspI StyI SwaI Tth111I XbaI