Restriction map for
pF1KA1555
(Linear) (Six-base) MAPSORT of: KIAA1555.seq Check: 6763 from: 1 to: 7215
KIAA1555.orf
With 182 enzymes: SgfI *
November 19, 2008 15:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1368 2190 2505 6542 6900 7215
Size: 1368 822 315 4037 358 315
Fragments arranged by size:
4037 1368 822 358 315 315
AatII G_ACGT'C
Cuts at: 0 5336 7215
Size: 5336 1879
Acc65I G'GTAC_C
Cuts at: 0 6169 7215
Size: 6169 1046
AclI AA'CG_TT
Cuts at: 0 2993 7215
Size: 2993 4222
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 872 1082 1780 1990 3633 3727 5326 6076
Size: 872 210 698 210 1643 94 1599 750
Cuts at: 6076 6510 7215
Size: 434 705
Fragments arranged by size:
1643 1599 872 750 705 698 434 210
210 94
AflII C'TTAA_G
Cuts at: 0 1101 7215
Size: 1101 6114
AflIII A'CryG_T
Cuts at: 0 1733 2921 4287 6147 7215
Size: 1733 1188 1366 1860 1068
Fragments arranged by size:
1860 1733 1366 1188 1068
AleI CACnn'nnGTG
Cuts at: 0 644 3940 7215
Size: 644 3296 3275
Fragments arranged by size:
3296 3275 644
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 936 968 7215
Size: 936 32 6247
Fragments arranged by size:
6247 936 32
AlwNI CAG_nnn'CTG
Cuts at: 0 850 1089 2053 3169 4054 5521 5783 6920
Size: 850 239 964 1116 885 1467 262 1137
Cuts at: 6920 7215
Size: 295
Fragments arranged by size:
1467 1137 1116 964 885 850 295 262
239
ApaI G_GGCC'C
Cuts at: 0 184 3310 3338 4133 5698 6067 6647 6702
Size: 184 3126 28 795 1565 369 580 55
Cuts at: 6702 7215
Size: 513
Fragments arranged by size:
3126 1565 795 580 513 369 184 55
28
ApaLI G'TGCA_C
Cuts at: 0 7004 7215
Size: 7004 211
ApoI r'AATT_y
Cuts at: 0 2387 3481 4532 5279 6781 7215
Size: 2387 1094 1051 747 1502 434
Fragments arranged by size:
2387 1502 1094 1051 747 434
AvaI C'yCGr_G
Cuts at: 0 177 460 2218 2434 2793 2954 3294 5999
Size: 177 283 1758 216 359 161 340 2705
Cuts at: 5999 6140 6163 6701 6849 7018 7215
Size: 141 23 538 148 169 197
Fragments arranged by size:
2705 1758 538 359 340 283 216 197
177 169 161 148 141 23
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 4765 4798 7215
Size: 4765 33 2417
Fragments arranged by size:
4765 2417 33
BamHI G'GATC_C
Cuts at: 0 3 800 6593 7215
Size: 3 797 5793 622
Fragments arranged by size:
5793 797 622 3
BanI G'GyrC_C
Cuts at: 0 25 1353 2032 2914 4695 5640 6169 6888
Size: 25 1328 679 882 1781 945 529 719
Cuts at: 6888 7215
Size: 327
Fragments arranged by size:
1781 1328 945 882 719 679 529 327
25
BanII G_rGCy'C
Cuts at: 0 145 158 184 413 827 893 1147 1468
Size: 145 13 26 229 414 66 254 321
Cuts at: 1468 1523 2989 3310 3338 4133 4708 5698 5845
Size: 55 1466 321 28 795 575 990 147
Cuts at: 5845 6067 6624 6647 6702 6745 6902 6985 7169
Size: 222 557 23 55 43 157 83 184
Cuts at: 7169 7215
Size: 46
Fragments arranged by size:
1466 990 795 575 557 414 321 321
254 229 222 184 157 147 145 83
66 55 55 46 43 28 26 23
13
BbeI G_GCGC'C
Cuts at: 0 5644 7215
Size: 5644 1571
BbsI GAAGACnn'nnnn_
Cuts at: 0 480 1013 1070 1343 2468 2644 3201 3695
Size: 480 533 57 273 1125 176 557 494
Cuts at: 3695 5588 5904 7215
Size: 1893 316 1311
Fragments arranged by size:
1893 1311 1125 557 533 494 480 316
273 176 57
BbvCI CC'TCA_GC
Cuts at: 0 38 3693 3888 5699 6015 6291 6370 7215
Size: 38 3655 195 1811 316 276 79 845
Fragments arranged by size:
3655 1811 845 316 276 195 79 38
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 296 330 4692 4726 7009 7043 7215
Size: 296 34 4362 34 2283 34 172
Fragments arranged by size:
4362 2283 296 172 34 34 34
BfrBI ATG'CAT
Cuts at: 0 2097 7215
Size: 2097 5118
BglI GCCn_nnn'nGGC
Cuts at: 0 674 2595 3085 3802 6032 6706 7215
Size: 674 1921 490 717 2230 674 509
Fragments arranged by size:
2230 1921 717 674 674 509 490
BglII A'GATC_T
Cuts at: 0 4019 5100 7215
Size: 4019 1081 2115
Fragments arranged by size:
4019 2115 1081
BlpI GC'TnA_GC
Cuts at: 0 1567 2835 4047 5762 5865 7215
Size: 1567 1268 1212 1715 103 1350
Fragments arranged by size:
1715 1567 1350 1268 1212 103
Bme1580I G_kGCm'C
Cuts at: 0 28 184 2294 2917 3310 3338 3347 4133
Size: 28 156 2110 623 393 28 9 786
Cuts at: 4133 5698 6067 6567 6647 6702 6891 7008 7176
Size: 1565 369 500 80 55 189 117 168
Cuts at: 7176 7215
Size: 39
Fragments arranged by size:
2110 1565 786 623 500 393 369 189
168 156 117 80 55 39 28 28
9
BmrI ACTGGGnnnn_n'
Cuts at: 0 975 990 1580 2116 2472 2873 3076 3726
Size: 975 15 590 536 356 401 203 650
Cuts at: 3726 4026 7215
Size: 300 3189
Fragments arranged by size:
3189 975 650 590 536 401 356 300
203 15
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 3176 6233 7215
Size: 3176 3057 982
Fragments arranged by size:
3176 3057 982
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 361 494 604 808 906 1558 2030 2272
Size: 361 133 110 204 98 652 472 242
Cuts at: 2272 3059 3146 3461 3562 4273 4716 5467 5569
Size: 787 87 315 101 711 443 751 102
Cuts at: 5569 6203 6747 6823 6999 7215
Size: 634 544 76 176 216
Fragments arranged by size:
787 751 711 652 634 544 472 443
361 315 242 216 204 176 133 110
102 101 98 87 76
Bpu10I CC'TnA_GC
Cuts at: 0 38 329 1048 1717 3693 3888 5699 6015
Size: 38 291 719 669 1976 195 1811 316
Cuts at: 6015 6291 6370 6770 7215
Size: 276 79 400 445
Fragments arranged by size:
1976 1811 719 669 445 400 316 291
276 195 79 38
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 4266 4405 5892 7215
Size: 4266 139 1487 1323
Fragments arranged by size:
4266 1487 1323 139
BsaI GGTCTCn'nnnn_
Cuts at: 0 369 516 5757 6086 6221 6379 6780 7215
Size: 369 147 5241 329 135 158 401 435
Fragments arranged by size:
5241 435 401 369 329 158 147 135
BsaAI yAC'GTr
Cuts at: 0 1734 6042 7215
Size: 1734 4308 1173
Fragments arranged by size:
4308 1734 1173
BsaBI GATnn'nnATC
Cuts at: 0 5191 6583 7215
Size: 5191 1392 632
Fragments arranged by size:
5191 1392 632
BsaHI Gr'CG_yC
Cuts at: 0 5333 5641 6960 7215
Size: 5333 308 1319 255
Fragments arranged by size:
5333 1319 308 255
BsaWI w'CCGG_w
Cuts at: 0 2344 7215
Size: 2344 4871
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 936 966 1510 1540 1825 1855 2395 2425
Size: 936 30 544 30 285 30 540 30
Cuts at: 2425 3596 3626 3673 3703 3731 3761 3888 3918
Size: 1171 30 47 30 28 30 127 30
Cuts at: 3918 4667 4697 5521 5551 7215
Size: 749 30 824 30 1664
Fragments arranged by size:
1664 1171 936 824 749 544 540 285
127 47 30 30 30 30 30 30
30 30 30 30 28
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 835 871 1825 2157 2382 2599 2659 3132
Size: 835 36 954 332 225 217 60 473
Cuts at: 3132 3681 3876 3896 4369 4372 5145 5161 5284
Size: 549 195 20 473 3 773 16 123
Cuts at: 5284 5464 5542 5614 5617 5670 6092 6358 6447
Size: 180 78 72 3 53 422 266 89
Cuts at: 6447 6529 6783 7215
Size: 82 254 432
Fragments arranged by size:
954 835 773 549 473 473 432 422
332 266 254 225 217 195 180 123
89 82 78 72 60 53 36 20
16 3 3
BseYI C'CCAG_C
Cuts at: 0 610 1310 1345 2660 2681 2688 2935 3617
Size: 610 700 35 1315 21 7 247 682
Cuts at: 3617 3633 3829 3876 4331 4604 4786 5296 5823
Size: 16 196 47 455 273 182 510 527
Cuts at: 5823 5981 6274 6548 7092 7215
Size: 158 293 274 544 123
Fragments arranged by size:
1315 700 682 610 544 527 510 455
293 274 273 247 196 182 158 123
47 35 21 16 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1217 1626 3475 3577 4130 6022 6526 6625
Size: 1217 409 1849 102 553 1892 504 99
Cuts at: 6625 6906 7215
Size: 281 309
Fragments arranged by size:
1892 1849 1217 553 504 409 309 281
102 99
BsiEI CG_ry'CG
Cuts at: 0 2599 5250 6949 7215
Size: 2599 2651 1699 266
Fragments arranged by size:
2651 2599 1699 266
BsiHKAI G_wGCw'C
Cuts at: 0 158 620 893 1043 1391 1468 1950 4708
Size: 158 462 273 150 348 77 482 2758
Cuts at: 4708 5534 6147 6155 6745 7008 7215
Size: 826 613 8 590 263 207
Fragments arranged by size:
2758 826 613 590 482 462 348 273
263 207 158 150 77 8
BsmI GAATG_Cn'
Cuts at: 0 307 7215
Size: 307 6908
Bsp1286I G_dGCh'C
Cuts at: 0 28 145 158 184 413 620 827 893
Size: 28 117 13 26 229 207 207 66
Cuts at: 893 1043 1147 1391 1468 1523 1950 2294 2917
Size: 150 104 244 77 55 427 344 623
Cuts at: 2917 2989 3310 3338 3347 4133 4708 5534 5698
Size: 72 321 28 9 786 575 826 164
Cuts at: 5698 5845 6067 6147 6155 6567 6624 6647 6702
Size: 147 222 80 8 412 57 23 55
Cuts at: 6702 6745 6891 6902 6985 7008 7169 7176 7215
Size: 43 146 11 83 23 161 7 39
Fragments arranged by size:
826 786 623 575 427 412 344 321
244 229 222 207 207 164 161 150
147 146 117 104 83 80 77 72
66 57 55 55 43 39 28 28
26 23 23 13 11 9 8 7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1197 1368 2190 2505 3422 6397 6542 6900
Size: 1197 171 822 315 917 2975 145 358
Cuts at: 6900 7215
Size: 315
Fragments arranged by size:
2975 1197 917 822 358 315 315 171
145
BsrBI CCG'CTC
Cuts at: 0 798 2834 7215
Size: 798 2036 4381
Fragments arranged by size:
4381 2036 798
BsrDI GCAATG_nn'
Cuts at: 0 2814 4004 7215
Size: 2814 1190 3211
Fragments arranged by size:
3211 2814 1190
BsrFI r'CCGG_y
Cuts at: 0 593 1770 2594 5178 5829 6838 7008 7215
Size: 593 1177 824 2584 651 1009 170 207
Fragments arranged by size:
2584 1177 1009 824 651 593 207 170
BsrGI T'GTAC_A
Cuts at: 0 4001 4901 7215
Size: 4001 900 2314
Fragments arranged by size:
4001 2314 900
BssSI C'ACGA_G
Cuts at: 0 3451 5738 7215
Size: 3451 2287 1477
Fragments arranged by size:
3451 2287 1477
BstAPI GCAn_nnn'nTGC
Cuts at: 0 3453 5426 7215
Size: 3453 1973 1789
Fragments arranged by size:
3453 1973 1789
BstBI TT'CG_AA
Cuts at: 0 5198 7215
Size: 5198 2017
BstEII G'GTnAC_C
Cuts at: 0 877 4026 7215
Size: 877 3149 3189
Fragments arranged by size:
3189 3149 877
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2910 4612 6263 7215
Size: 2910 1702 1651 952
Fragments arranged by size:
2910 1702 1651 952
BstYI r'GATC_y
Cuts at: 0 3 800 2570 4019 4512 5100 6593 7215
Size: 3 797 1770 1449 493 588 1493 622
Fragments arranged by size:
1770 1493 1449 797 622 588 493 3
Bsu36I CC'TnA_GG
Cuts at: 0 753 2624 4951 5009 7152 7190 7215
Size: 753 1871 2327 58 2143 38 25
Fragments arranged by size:
2327 2143 1871 753 58 38 25
BtgI C'CryG_G
Cuts at: 0 1421 2080 2309 2859 3938 4322 4563 4988
Size: 1421 659 229 550 1079 384 241 425
Cuts at: 4988 5244 5902 6241 6328 6400 6489 6876 7215
Size: 256 658 339 87 72 89 387 339
Fragments arranged by size:
1421 1079 659 658 550 425 387 384
339 339 256 241 229 89 87 72
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 6000 7215
Size: 6000 1215
BtsI GCAGTG_nn'
Cuts at: 0 1561 3358 3852 4410 5665 7215
Size: 1561 1797 494 558 1255 1550
Fragments arranged by size:
1797 1561 1550 1255 558 494
ClaI AT'CG_AT
Cuts at: 0 1124 7215
Size: 1124 6091
DraI TTT'AAA
Cuts at: 0 4826 5373 7215
Size: 4826 547 1842
Fragments arranged by size:
4826 1842 547
DraIII CAC_nnn'GTG
Cuts at: 0 3286 7215
Size: 3286 3929
EaeI y'GGCC_r
Cuts at: 0 2596 3272 4991 5247 6492 6613 6946 7010
Size: 2596 676 1719 256 1245 121 333 64
Cuts at: 7010 7215
Size: 205
Fragments arranged by size:
2596 1719 1245 676 333 256 205 121
64
EagI C'GGCC_G
Cuts at: 0 2596 5247 6946 7215
Size: 2596 2651 1699 269
Fragments arranged by size:
2651 2596 1699 269
EarI CTCTTCn'nnn_
Cuts at: 0 524 806 848 2134 2149 2168 2710 4148
Size: 524 282 42 1286 15 19 542 1438
Cuts at: 4148 4429 4623 5120 5216 6120 6343 7215
Size: 281 194 497 96 904 223 872
Fragments arranged by size:
1438 1286 904 872 542 524 497 282
281 223 194 96 42 19 15
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1476 2533 6462 6857 7215
Size: 1476 1057 3929 395 358
Fragments arranged by size:
3929 1476 1057 395 358
EcoICRI GAG'CTC
Cuts at: 0 156 891 1466 4706 6743 7215
Size: 156 735 575 3240 2037 472
Fragments arranged by size:
3240 2037 735 575 472 156
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 361 494 604 808 872 906 1082 1558
Size: 361 133 110 204 64 34 176 476
Cuts at: 1558 1780 1990 2030 2272 3059 3146 3461 3562
Size: 222 210 40 242 787 87 315 101
Cuts at: 3562 3633 3727 4273 4716 5326 5467 5569 6076
Size: 71 94 546 443 610 141 102 507
Cuts at: 6076 6203 6510 6747 6823 6999 7215
Size: 127 307 237 76 176 216
Fragments arranged by size:
787 610 546 507 476 443 361 315
307 242 237 222 216 210 204 176
176 141 133 127 110 102 101 94
87 76 71 64 40 34
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1790 5012 5488 7072 7215
Size: 1790 3222 476 1584 143
Fragments arranged by size:
3222 1790 1584 476 143
EcoO109I rG'GnC_Cy
Cuts at: 0 181 248 465 653 2305 2874 3064 3306
Size: 181 67 217 188 1652 569 190 242
Cuts at: 3306 3323 3334 4868 5695 6064 6643 6698 7215
Size: 17 11 1534 827 369 579 55 517
Fragments arranged by size:
1652 1534 827 579 569 517 369 242
217 190 188 181 67 55 17 11
EcoRI G'AATT_C
Cuts at: 0 3481 5279 7215
Size: 3481 1798 1936
Fragments arranged by size:
3481 1936 1798
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 4184 6285 7215
Size: 4184 2101 930
Fragments arranged by size:
4184 2101 930
HaeII r_GCGC'y
Cuts at: 0 1767 5644 5769 6253 7215
Size: 1767 3877 125 484 962
Fragments arranged by size:
3877 1767 962 484 125
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1510 1542 1825 1857 2068 2100 4672 4704
Size: 1510 32 283 32 211 32 2572 32
Cuts at: 4704 4852 4884 5545 5551 5577 5583 5605 5637
Size: 148 32 661 6 26 6 22 32
Cuts at: 5637 5848 5880 6730 6762 7215
Size: 211 32 850 32 453
Fragments arranged by size:
2572 1510 850 661 453 283 211 211
148 32 32 32 32 32 32 32
32 26 22 6 6
HincII GTy'rAC
Cuts at: 0 366 2927 4637 7215
Size: 366 2561 1710 2578
Fragments arranged by size:
2578 2561 1710 366
HindIII A'AGCT_T
Cuts at: 0 4586 7215
Size: 4586 2629
KasI G'GCGC_C
Cuts at: 0 5640 7215
Size: 5640 1575
KpnI G_GTAC'C
Cuts at: 0 6173 7215
Size: 6173 1042
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 3155 7215
Size: 3155 4060
MscI TGG'CCA
Cuts at: 0 4993 7215
Size: 4993 2222
MslI CAynn'nnrTG
Cuts at: 0 644 2920 2948 3940 7215
Size: 644 2276 28 992 3275
Fragments arranged by size:
3275 2276 992 644 28
MspA1I CmG'CkG
Cuts at: 0 119 127 926 1281 2692 4303 5246 5783
Size: 119 8 799 355 1411 1611 943 537
Cuts at: 5783 6096 6295 6725 7041 7215
Size: 313 199 430 316 174
Fragments arranged by size:
1611 1411 943 799 537 430 355 316
313 199 174 119 8
NaeI GCC'GGC
Cuts at: 0 595 1772 5180 7215
Size: 595 1177 3408 2035
Fragments arranged by size:
3408 2035 1177 595
NarI GG'CG_CC
Cuts at: 0 5641 7215
Size: 5641 1574
NcoI C'CATG_G
Cuts at: 0 2859 3938 4322 4563 4988 6876 7215
Size: 2859 1079 384 241 425 1888 339
Fragments arranged by size:
2859 1888 1079 425 384 339 241
NdeI CA'TA_TG
Cuts at: 0 1939 7215
Size: 1939 5276
NgoMIV G'CCGG_C
Cuts at: 0 593 1770 5178 7215
Size: 593 1177 3408 2037
Fragments arranged by size:
3408 2037 1177 593
NsiI A_TGCA'T
Cuts at: 0 2099 7215
Size: 2099 5116
NspI r_CATG'y
Cuts at: 0 2898 2925 2953 4858 5304 5665 5808 6151
Size: 2898 27 28 1905 446 361 143 343
Cuts at: 6151 7215
Size: 1064
Fragments arranged by size:
2898 1905 1064 446 361 343 143 28
27
PasI CC'CwG_GG
Cuts at: 0 406 805 2910 3310 6120 6954 7169 7215
Size: 406 399 2105 400 2810 834 215 46
Fragments arranged by size:
2810 2105 834 406 400 399 215 46
PciI A'CATG_T
Cuts at: 0 2921 6147 7215
Size: 2921 3226 1068
Fragments arranged by size:
3226 2921 1068
PflMI CCAn_nnn'nTGG
Cuts at: 0 4023 6212 6501 7215
Size: 4023 2189 289 714
Fragments arranged by size:
4023 2189 714 289
PfoI T'CCnGG_A
Cuts at: 0 338 471 5489 6976 7215
Size: 338 133 5018 1487 239
Fragments arranged by size:
5018 1487 338 239 133
PmlI CAC'GTG
Cuts at: 0 1734 6042 7215
Size: 1734 4308 1173
Fragments arranged by size:
4308 1734 1173
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 936 968 7215
Size: 936 32 6247
Fragments arranged by size:
6247 936 32
PpuMI rG'GwC_Cy
Cuts at: 0 465 2305 4868 7215
Size: 465 1840 2563 2347
Fragments arranged by size:
2563 2347 1840 465
PshAI GACnn'nnGTC
Cuts at: 0 5904 7215
Size: 5904 1311
PspOMI G'GGCC_C
Cuts at: 0 180 3306 3334 4129 5694 6063 6643 6698
Size: 180 3126 28 795 1565 369 580 55
Cuts at: 6698 7215
Size: 517
Fragments arranged by size:
3126 1565 795 580 517 369 180 55
28
PspXI vC'TCGA_Gb
Cuts at: 0 6140 6163 7215
Size: 6140 23 1052
Fragments arranged by size:
6140 1052 23
PstI C_TGCA'G
Cuts at: 0 592 888 2031 2531 4781 6897 7215
Size: 592 296 1143 500 2250 2116 318
Fragments arranged by size:
2250 2116 1143 592 500 318 296
PvuII CAG'CTG
Cuts at: 0 127 2692 4303 5783 6096 7215
Size: 127 2565 1611 1480 313 1119
Fragments arranged by size:
2565 1611 1480 1119 313 127
RsrII CG'GwC_CG
Cuts at: 0 1283 7215
Size: 1283 5932
SacI G_AGCT'C
Cuts at: 0 158 893 1468 4708 6745 7215
Size: 158 735 575 3240 2037 470
Fragments arranged by size:
3240 2037 735 575 470 158
SacII CC_GC'GG
Cuts at: 0 5247 7215
Size: 5247 1968
SanDI GG'GwC_CC
Cuts at: 0 2305 7215
Size: 2305 4910
SapI GCTCTTCn'nnn_
Cuts at: 0 2149 2168 4148 7215
Size: 2149 19 1980 3067
Fragments arranged by size:
3067 2149 1980 19
ScaI AGT'ACT
Cuts at: 0 586 1164 7215
Size: 586 578 6051
Fragments arranged by size:
6051 586 578
SfcI C'TryA_G
Cuts at: 0 588 884 2027 2527 4777 6893 7215
Size: 588 296 1143 500 2250 2116 322
Fragments arranged by size:
2250 2116 1143 588 500 322 296
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 6706 7215
Size: 6706 509
SfoI GGC'GCC
Cuts at: 0 5642 7215
Size: 5642 1573
SmaI CCC'GGG
Cuts at: 0 179 2220 3296 6703 6851 7215
Size: 179 2041 1076 3407 148 364
Fragments arranged by size:
3407 2041 1076 364 179 148
SmlI C'TyrA_G
Cuts at: 0 1101 2434 4245 4384 5871 6140 6163 7215
Size: 1101 1333 1811 139 1487 269 23 1052
Fragments arranged by size:
1811 1487 1333 1101 1052 269 139 23
SpeI A'CTAG_T
Cuts at: 0 862 7215
Size: 862 6353
SphI G_CATG'C
Cuts at: 0 4858 5304 5808 7215
Size: 4858 446 504 1407
Fragments arranged by size:
4858 1407 504 446
SrfI GCCC'GGGC
Cuts at: 0 6851 7215
Size: 6851 364
StuI AGG'CCT
Cuts at: 0 746 3145 3159 6013 6185 6858 7078 7215
Size: 746 2399 14 2854 172 673 220 137
Fragments arranged by size:
2854 2399 746 673 220 172 137 14
StyI C'CwwG_G
Cuts at: 0 1456 1868 2160 2297 2323 2859 3188 3938
Size: 1456 412 292 137 26 536 329 750
Cuts at: 3938 4038 4322 4367 4470 4563 4667 4691 4988
Size: 100 284 45 103 93 104 24 297
Cuts at: 4988 6269 6821 6876 7215
Size: 1281 552 55 339
Fragments arranged by size:
1456 1281 750 552 536 412 339 329
297 292 284 137 104 103 100 93
55 45 26 24
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 148 751 1084 1324 2050 2721 2932 2960
Size: 148 603 333 240 726 671 211 28
Cuts at: 2960 3031 3508 3611 3647 4045 5338 5700 5739
Size: 71 477 103 36 398 1293 362 39
Cuts at: 5739 6921 7215
Size: 1182 294
Fragments arranged by size:
1293 1182 726 671 603 477 398 362
333 294 240 211 148 103 71 39
36 28
TatI w'GTAC_w
Cuts at: 0 572 584 1162 3839 4001 4901 7215
Size: 572 12 578 2677 162 900 2314
Fragments arranged by size:
2677 2314 900 578 572 162 12
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 703 735 2395 2427 3731 3763 5009 5041
Size: 703 32 1660 32 1304 32 1246 32
Cuts at: 5041 7215
Size: 2174
Fragments arranged by size:
2174 1660 1304 1246 703 32 32 32
32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 357 3945 5900 7038 7215
Size: 357 3588 1955 1138 177
Fragments arranged by size:
3588 1955 1138 357 177
XhoI C'TCGA_G
Cuts at: 0 2434 6140 6163 7215
Size: 2434 3706 23 1052
Fragments arranged by size:
3706 2434 1052 23
XmaI C'CCGG_G
Cuts at: 0 177 2218 3294 6701 6849 7215
Size: 177 2041 1076 3407 148 366
Fragments arranged by size:
3407 2041 1076 366 177 148
XmnI GAAnn'nnTTC
Cuts at: 0 6523 7215
Size: 6523 692
ZraI GAC'GTC
Cuts at: 0 5334 7215
Size: 5334 1881
Enzymes that do cut:
AarI AatII Acc65I AclI AcuI AflII AflIII AleI
AloI AlwNI ApaI ApaLI ApoI AvaI BaeI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BfrBI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI DraI DraIII EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI HaeII Hin4I HincII HindIII KasI KpnI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NsiI NspI PasI PciI PflMI PfoI PmlI PpiI
PpuMI PshAI PspOMI PspXI PstI PvuII RsrII SacI
SacII SanDI SapI ScaI SfcI SfiI SfoI SmaI
SmlI SpeI SphI SrfI StuI StyI TaqII TatI
TstI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AccI AfeI AgeI AhdI AlfI AscI AseI AsiSI
AvrII BciVI BclI BmgBI BmtI BsiWI BsmBI BspEI
BspHI BssHII BstZ17I CspCI DrdI EcoRV FseI FspI
FspAI HpaI MfeI MluI NheI NotI NruI PacI
PmeI PsiI PsrI PvuI SalI SbfI SexAI SgfI
SgrAI SnaBI SspI SwaI Tth111I XbaI