Restriction map for
pF1KA1560
(Linear) (Six-base) MAPSORT of: KIAA1560.seq Check: 4298 from: 1 to: 2484
KIAA1560.orf
With 182 enzymes: SgfI *
March 2, 2010 14:24 ..
AccI GT'mk_AC
Cuts at: 0 1018 2484
Size: 1018 1466
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 114 746 774 2137 2208 2484
Size: 114 632 28 1363 71 276
Fragments arranged by size:
1363 632 276 114 71 28
AflIII A'CryG_T
Cuts at: 0 949 1337 2484
Size: 949 388 1147
Fragments arranged by size:
1147 949 388
AgeI A'CCGG_T
Cuts at: 0 1248 2484
Size: 1248 1236
AleI CACnn'nnGTG
Cuts at: 0 89 382 2484
Size: 89 293 2102
Fragments arranged by size:
2102 293 89
AlwNI CAG_nnn'CTG
Cuts at: 0 1617 2245 2484
Size: 1617 628 239
Fragments arranged by size:
1617 628 239
ApoI r'AATT_y
Cuts at: 0 229 614 660 1581 2484
Size: 229 385 46 921 903
Fragments arranged by size:
921 903 385 229 46
AseI AT'TA_AT
Cuts at: 0 1346 2484
Size: 1346 1138
AvaI C'yCGr_G
Cuts at: 0 980 2484
Size: 980 1504
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 228 261 2233 2266 2484
Size: 228 33 1972 33 218
Fragments arranged by size:
1972 228 218 33 33
BanI G'GyrC_C
Cuts at: 0 1888 2484
Size: 1888 596
BbsI GAAGACnn'nnnn_
Cuts at: 0 1526 1689 2084 2484
Size: 1526 163 395 400
Fragments arranged by size:
1526 400 395 163
BciVI GTATCCnnnnn_n'
Cuts at: 0 256 2484
Size: 256 2228
BclI T'GATC_A
Cuts at: 0 554 1826 2484
Size: 554 1272 658
Fragments arranged by size:
1272 658 554
BfrBI ATG'CAT
Cuts at: 0 1602 2484
Size: 1602 882
BglII A'GATC_T
Cuts at: 0 133 932 2484
Size: 133 799 1552
Fragments arranged by size:
1552 799 133
BlpI GC'TnA_GC
Cuts at: 0 1406 2015 2484
Size: 1406 609 469
Fragments arranged by size:
1406 609 469
BmgBI CAC'GTC
Cuts at: 0 1340 2484
Size: 1340 1144
BmrI ACTGGGnnnn_n'
Cuts at: 0 236 575 1114 1304 1804 2484
Size: 236 339 539 190 500 680
Fragments arranged by size:
680 539 500 339 236 190
BmtI G_CTAG'C
Cuts at: 0 1430 2484
Size: 1430 1054
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 901 2484
Size: 901 1583
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1291 2206 2484
Size: 1291 915 278
Fragments arranged by size:
1291 915 278
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 652 2484
Size: 652 1832
BsaI GGTCTCn'nnnn_
Cuts at: 0 2374 2484
Size: 2374 110
BsaHI Gr'CG_yC
Cuts at: 0 321 2484
Size: 321 2163
BsaWI w'CCGG_w
Cuts at: 0 1248 1952 2484
Size: 1248 704 532
Fragments arranged by size:
1248 704 532
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1264 1294 1861 1891 2153 2183 2484
Size: 1264 30 567 30 262 30 301
Fragments arranged by size:
1264 567 301 262 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1465 2484
Size: 1465 1019
BseYI C'CCAG_C
Cuts at: 0 221 467 1617 1675 2484
Size: 221 246 1150 58 809
Fragments arranged by size:
1150 809 246 221 58
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 192 2484
Size: 192 2292
BsiHKAI G_wGCw'C
Cuts at: 0 981 1982 2141 2484
Size: 981 1001 159 343
Fragments arranged by size:
1001 981 343 159
BsmI GAATG_Cn'
Cuts at: 0 1961 2346 2484
Size: 1961 385 138
Fragments arranged by size:
1961 385 138
BsmBI CGTCTCn'nnnn_
Cuts at: 0 649 2484
Size: 649 1835
Bsp1286I G_dGCh'C
Cuts at: 0 981 1982 2141 2484
Size: 981 1001 159 343
Fragments arranged by size:
1001 981 343 159
BsrDI GCAATG_nn'
Cuts at: 0 557 755 1725 2484
Size: 557 198 970 759
Fragments arranged by size:
970 759 557 198
BsrFI r'CCGG_y
Cuts at: 0 546 1248 2484
Size: 546 702 1236
Fragments arranged by size:
1236 702 546
BssSI C'ACGA_G
Cuts at: 0 1553 2484
Size: 1553 931
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1845 2262 2308 2484
Size: 1845 417 46 176
Fragments arranged by size:
1845 417 176 46
BstBI TT'CG_AA
Cuts at: 0 1700 2484
Size: 1700 784
BstXI CCAn_nnnn'nTGG
Cuts at: 0 811 2484
Size: 811 1673
BstYI r'GATC_y
Cuts at: 0 133 691 932 2484
Size: 133 558 241 1552
Fragments arranged by size:
1552 558 241 133
BtgI C'CryG_G
Cuts at: 0 303 888 2484
Size: 303 585 1596
Fragments arranged by size:
1596 585 303
BtsI GCAGTG_nn'
Cuts at: 0 1421 1897 2029 2484
Size: 1421 476 132 455
Fragments arranged by size:
1421 476 455 132
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1440 1475 2208 2243 2484
Size: 1440 35 733 35 241
Fragments arranged by size:
1440 733 241 35 35
DraI TTT'AAA
Cuts at: 0 146 2484
Size: 146 2338
DraIII CAC_nnn'GTG
Cuts at: 0 1461 2484
Size: 1461 1023
DrdI GACnn_nn'nnGTC
Cuts at: 0 1799 2484
Size: 1799 685
EaeI y'GGCC_r
Cuts at: 0 1856 2484
Size: 1856 628
EarI CTCTTCn'nnn_
Cuts at: 0 733 2484
Size: 733 1751
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 114 746 774 1291 2137 2206 2208 2484
Size: 114 632 28 517 846 69 2 276
Fragments arranged by size:
846 632 517 276 114 69 28 2
EcoO109I rG'GnC_Cy
Cuts at: 0 1801 2484
Size: 1801 683
EcoRV GAT'ATC
Cuts at: 0 1998 2484
Size: 1998 486
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 646 2484
Size: 646 1838
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 110 142 821 853 1000 1032 1383 1415
Size: 110 32 679 32 147 32 351 32
Cuts at: 1415 2484
Size: 1069
Fragments arranged by size:
1069 679 351 147 110 32 32 32
32
HincII GTy'rAC
Cuts at: 0 489 628 2484
Size: 489 139 1856
Fragments arranged by size:
1856 489 139
HindIII A'AGCT_T
Cuts at: 0 808 2035 2484
Size: 808 1227 449
Fragments arranged by size:
1227 808 449
HpaI GTT'AAC
Cuts at: 0 489 2484
Size: 489 1995
MfeI C'AATT_G
Cuts at: 0 425 2484
Size: 425 2059
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 151 168 1035 2484
Size: 151 17 867 1449
Fragments arranged by size:
1449 867 151 17
MslI CAynn'nnrTG
Cuts at: 0 89 382 1091 2484
Size: 89 293 709 1393
Fragments arranged by size:
1393 709 293 89
MspA1I CmG'CkG
Cuts at: 0 305 1614 1842 2484
Size: 305 1309 228 642
Fragments arranged by size:
1309 642 305 228
NcoI C'CATG_G
Cuts at: 0 888 2484
Size: 888 1596
NheI G'CTAG_C
Cuts at: 0 1426 2484
Size: 1426 1058
NsiI A_TGCA'T
Cuts at: 0 1604 2484
Size: 1604 880
NspI r_CATG'y
Cuts at: 0 372 1606 2484
Size: 372 1234 878
Fragments arranged by size:
1234 878 372
PflMI CCAn_nnn'nTGG
Cuts at: 0 187 2484
Size: 187 2297
PfoI T'CCnGG_A
Cuts at: 0 938 2484
Size: 938 1546
PpuMI rG'GwC_Cy
Cuts at: 0 1801 2484
Size: 1801 683
PshAI GACnn'nnGTC
Cuts at: 0 993 2484
Size: 993 1491
PvuII CAG'CTG
Cuts at: 0 1614 1842 2484
Size: 1614 228 642
Fragments arranged by size:
1614 642 228
SacII CC_GC'GG
Cuts at: 0 306 2484
Size: 306 2178
SanDI GG'GwC_CC
Cuts at: 0 1801 2484
Size: 1801 683
SfcI C'TryA_G
Cuts at: 0 873 1479 1713 2193 2484
Size: 873 606 234 480 291
Fragments arranged by size:
873 606 480 291 234
SmlI C'TyrA_G
Cuts at: 0 631 2484
Size: 631 1853
SphI G_CATG'C
Cuts at: 0 1606 2484
Size: 1606 878
SspI AAT'ATT
Cuts at: 0 1225 2484
Size: 1225 1259
StuI AGG'CCT
Cuts at: 0 2191 2484
Size: 2191 293
StyI C'CwwG_G
Cuts at: 0 21 888 2132 2172 2484
Size: 21 867 1244 40 312
Fragments arranged by size:
1244 867 312 40 21
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 557 561 1654 2484
Size: 557 4 1093 830
Fragments arranged by size:
1093 830 557 4
TatI w'GTAC_w
Cuts at: 0 414 2484
Size: 414 2070
XbaI T'CTAG_A
Cuts at: 0 2454 2484
Size: 2454 30
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1458 1614 2484
Size: 1458 156 870
Fragments arranged by size:
1458 870 156
XmnI GAAnn'nnTTC
Cuts at: 0 908 2462 2484
Size: 908 1554 22
Fragments arranged by size:
1554 908 22
Enzymes that do cut:
AccI AcuI AflIII AgeI AleI AlwNI ApoI AseI
AvaI BaeI BanI BbsI BciVI BclI BfrBI BglII
BlpI BmgBI BmrI BmtI BplI BpmI BpuEI BsaI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI
BsmBI Bsp1286I BsrDI BsrFI BssSI BstAPI BstBI BstXI
BstYI BtgI BtsI CspCI DraI DraIII DrdI EaeI
EarI Eco57MI EcoO109I EcoRV FalI Hin4I HincII HindIII
HpaI MfeI MmeI MslI MspA1I NcoI NheI NsiI
NspI PflMI PfoI PpuMI PshAI PvuII SacII SanDI
SfcI SmlI SphI SspI StuI StyI TaqII TatI
XbaI XcmI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AhdI AlfI
AloI ApaI ApaLI AscI AsiSI AvrII BamHI BanII
BbeI BbvCI BcgI BglI Bme1580I Bpu10I BsaAI BsaBI
BsiEI BsiWI BspEI BspHI BspMI BsrBI BsrGI BssHII
BstEII BstZ17I Bsu36I BtgZI ClaI EagI EciI EcoICRI
EcoNI EcoRI FseI FspI FspAI HaeII KasI KpnI
MluI MscI NaeI NarI NdeI NgoMIV NotI NruI
PacI PasI PciI PmeI PmlI PpiI PsiI PspOMI
PspXI PsrI PstI PvuI RsrII SacI SalI SapI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SrfI SwaI TstI Tth111I XhoI XmaI
ZraI