Restriction map for
pF1KSDA1561
(Linear) (Six-base) MAPSORT of: KIAA1561.seq Check: 9208 from: 1 to: 4248
KIAA1561.orf
With 182 enzymes: SgfI *
February 28, 2007 11:29 ..
AatII G_ACGT'C
Cuts at: 0 154 4248
Size: 154 4094
AccI GT'mk_AC
Cuts at: 0 494 1236 4248
Size: 494 742 3012
Fragments arranged by size:
3012 742 494
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1063 1402 1645 1652 3103 3424 3595 4248
Size: 1063 339 243 7 1451 321 171 653
Fragments arranged by size:
1451 1063 653 339 321 243 171 7
AflII C'TTAA_G
Cuts at: 0 1086 2373 2467 2488 4248
Size: 1086 1287 94 21 1760
Fragments arranged by size:
1760 1287 1086 94 21
AflIII A'CryG_T
Cuts at: 0 2621 3330 4248
Size: 2621 709 918
Fragments arranged by size:
2621 918 709
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 469 4248
Size: 469 3779
ApoI r'AATT_y
Cuts at: 0 825 935 1319 1891 2665 2686 2989 4248
Size: 825 110 384 572 774 21 303 1259
Fragments arranged by size:
1259 825 774 572 384 303 110 21
BanI G'GyrC_C
Cuts at: 0 58 4248
Size: 58 4190
BanII G_rGCy'C
Cuts at: 0 1912 2800 2921 4248
Size: 1912 888 121 1327
Fragments arranged by size:
1912 1327 888 121
BbeI G_GCGC'C
Cuts at: 0 62 4248
Size: 62 4186
BbsI GAAGACnn'nnnn_
Cuts at: 0 204 763 2258 2513 4248
Size: 204 559 1495 255 1735
Fragments arranged by size:
1735 1495 559 255 204
BclI T'GATC_A
Cuts at: 0 1134 4248
Size: 1134 3114
BfrBI ATG'CAT
Cuts at: 0 340 1492 2939 4248
Size: 340 1152 1447 1309
Fragments arranged by size:
1447 1309 1152 340
BglI GCCn_nnn'nGGC
Cuts at: 0 427 4248
Size: 427 3821
BglII A'GATC_T
Cuts at: 0 205 1258 4248
Size: 205 1053 2990
Fragments arranged by size:
2990 1053 205
BlpI GC'TnA_GC
Cuts at: 0 2036 4248
Size: 2036 2212
Bme1580I G_kGCm'C
Cuts at: 0 41 4248
Size: 41 4207
BmgBI CAC'GTC
Cuts at: 0 36 2043 4248
Size: 36 2007 2205
Fragments arranged by size:
2205 2007 36
BmrI ACTGGGnnnn_n'
Cuts at: 0 1286 4248
Size: 1286 2962
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 326 4248
Size: 326 3922
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 4117 4198 4248
Size: 4117 81 50
Fragments arranged by size:
4117 81 50
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 262 2032 2170 3579 4248
Size: 262 1770 138 1409 669
Fragments arranged by size:
1770 1409 669 262 138
BsaBI GATnn'nnATC
Cuts at: 0 2678 4248
Size: 2678 1570
BsaHI Gr'CG_yC
Cuts at: 0 59 151 4248
Size: 59 92 4097
Fragments arranged by size:
4097 92 59
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 560 590 1370 1400 4248
Size: 560 30 780 30 2848
Fragments arranged by size:
2848 780 560 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 39 581 2173 2989 4248
Size: 39 542 1592 816 1259
Fragments arranged by size:
1592 1259 816 542 39
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 392 1525 2845 4157 4248
Size: 392 1133 1320 1312 91
Fragments arranged by size:
1320 1312 1133 392 91
BsiHKAI G_wGCw'C
Cuts at: 0 1912 2783 2800 2921 4248
Size: 1912 871 17 121 1327
Fragments arranged by size:
1912 1327 871 121 17
BsmI GAATG_Cn'
Cuts at: 0 258 486 1423 2941 4248
Size: 258 228 937 1518 1307
Fragments arranged by size:
1518 1307 937 258 228
Bsp1286I G_dGCh'C
Cuts at: 0 41 1912 2783 2800 2921 4248
Size: 41 1871 871 17 121 1327
Fragments arranged by size:
1871 1327 871 121 41 17
BspHI T'CATG_A
Cuts at: 0 3162 4248
Size: 3162 1086
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 401 4248
Size: 401 3847
BsrBI CCG'CTC
Cuts at: 0 571 4248
Size: 571 3677
BsrDI GCAATG_nn'
Cuts at: 0 431 1349 4248
Size: 431 918 2899
Fragments arranged by size:
2899 918 431
BssHII G'CGCG_C
Cuts at: 0 74 4248
Size: 74 4174
BssSI C'ACGA_G
Cuts at: 0 2782 4248
Size: 2782 1466
BstAPI GCAn_nnn'nTGC
Cuts at: 0 36 4248
Size: 36 4212
BstYI r'GATC_y
Cuts at: 0 205 1258 4248
Size: 205 1053 2990
Fragments arranged by size:
2990 1053 205
BstZ17I GTA'TAC
Cuts at: 0 1237 4248
Size: 1237 3011
Bsu36I CC'TnA_GG
Cuts at: 0 22 1596 4248
Size: 22 1574 2652
Fragments arranged by size:
2652 1574 22
BtgI C'CryG_G
Cuts at: 0 465 4248
Size: 465 3783
BtsI GCAGTG_nn'
Cuts at: 0 293 2884 3311 4248
Size: 293 2591 427 937
Fragments arranged by size:
2591 937 427 293
DraI TTT'AAA
Cuts at: 0 1119 1325 2240 2994 4248
Size: 1119 206 915 754 1254
Fragments arranged by size:
1254 1119 915 754 206
DraIII CAC_nnn'GTG
Cuts at: 0 1410 1984 4086 4248
Size: 1410 574 2102 162
Fragments arranged by size:
2102 1410 574 162
EaeI y'GGCC_r
Cuts at: 0 696 2849 3740 4109 4248
Size: 696 2153 891 369 139
Fragments arranged by size:
2153 891 696 369 139
EarI CTCTTCn'nnn_
Cuts at: 0 317 1504 1718 1900 2591 2867 3400 3489
Size: 317 1187 214 182 691 276 533 89
Cuts at: 3489 4248
Size: 759
Fragments arranged by size:
1187 759 691 533 317 276 214 182
89
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 516 4248
Size: 516 3732
EcoICRI GAG'CTC
Cuts at: 0 1910 2798 2919 4248
Size: 1910 888 121 1329
Fragments arranged by size:
1910 1329 888 121
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1063 1402 1645 1652 3103 3424 3595 4117
Size: 1063 339 243 7 1451 321 171 522
Cuts at: 4117 4198 4248
Size: 81 50
Fragments arranged by size:
1451 1063 522 339 321 243 171 81
50 7
EcoO109I rG'GnC_Cy
Cuts at: 0 470 4248
Size: 470 3778
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1198 4248
Size: 1198 3050
HaeII r_GCGC'y
Cuts at: 0 62 4248
Size: 62 4186
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 861 893 1370 1402 2427 2459 2553 2585
Size: 861 32 477 32 1025 32 94 32
Cuts at: 2585 3010 3042 4056 4088 4248
Size: 425 32 1014 32 160
Fragments arranged by size:
1025 1014 861 477 425 160 94 32
32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 550 2123 3672 4248
Size: 550 1573 1549 576
Fragments arranged by size:
1573 1549 576 550
HindIII A'AGCT_T
Cuts at: 0 678 1083 1424 2029 2299 4248
Size: 678 405 341 605 270 1949
Fragments arranged by size:
1949 678 605 405 341 270
HpaI GTT'AAC
Cuts at: 0 2123 4248
Size: 2123 2125
KasI G'GCGC_C
Cuts at: 0 58 4248
Size: 58 4190
MfeI C'AATT_G
Cuts at: 0 369 2210 2960 3380 3460 4248
Size: 369 1841 750 420 80 788
Fragments arranged by size:
1841 788 750 420 369 80
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2318 3446 3519 3596 4248
Size: 2318 1128 73 77 652
Fragments arranged by size:
2318 1128 652 77 73
MscI TGG'CCA
Cuts at: 0 698 2851 3742 4248
Size: 698 2153 891 506
Fragments arranged by size:
2153 891 698 506
MslI CAynn'nnrTG
Cuts at: 0 1437 1831 4187 4225 4248
Size: 1437 394 2356 38 23
Fragments arranged by size:
2356 1437 394 38 23
MspA1I CmG'CkG
Cuts at: 0 453 990 1885 2478 4062 4107 4248
Size: 453 537 895 593 1584 45 141
Fragments arranged by size:
1584 895 593 537 453 141 45
NarI GG'CG_CC
Cuts at: 0 59 4248
Size: 59 4189
NcoI C'CATG_G
Cuts at: 0 465 4248
Size: 465 3783
NdeI CA'TA_TG
Cuts at: 0 1247 4248
Size: 1247 3001
NsiI A_TGCA'T
Cuts at: 0 342 1494 2941 4248
Size: 342 1152 1447 1307
Fragments arranged by size:
1447 1307 1152 342
NspI r_CATG'y
Cuts at: 0 82 340 388 840 2893 3334 3790 4248
Size: 82 258 48 452 2053 441 456 458
Fragments arranged by size:
2053 458 456 452 441 258 82 48
PacI TTA_AT'TAA
Cuts at: 0 659 4248
Size: 659 3589
PciI A'CATG_T
Cuts at: 0 3330 4248
Size: 3330 918
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 938 970 1271 1303 4248
Size: 938 32 301 32 2945
Fragments arranged by size:
2945 938 301 32 32
PstI C_TGCA'G
Cuts at: 0 300 398 4079 4248
Size: 300 98 3681 169
Fragments arranged by size:
3681 300 169 98
PvuII CAG'CTG
Cuts at: 0 453 990 1885 2478 4062 4107 4248
Size: 453 537 895 593 1584 45 141
Fragments arranged by size:
1584 895 593 537 453 141 45
SacI G_AGCT'C
Cuts at: 0 1912 2800 2921 4248
Size: 1912 888 121 1327
Fragments arranged by size:
1912 1327 888 121
SapI GCTCTTCn'nnn_
Cuts at: 0 317 1900 4248
Size: 317 1583 2348
Fragments arranged by size:
2348 1583 317
SbfI CC_TGCA'GG
Cuts at: 0 398 4248
Size: 398 3850
SfcI C'TryA_G
Cuts at: 0 296 361 394 2856 4075 4248
Size: 296 65 33 2462 1219 173
Fragments arranged by size:
2462 1219 296 173 65 33
SfoI GGC'GCC
Cuts at: 0 60 4248
Size: 60 4188
SmlI C'TyrA_G
Cuts at: 0 10 241 1086 2011 2185 2373 2467 2488
Size: 10 231 845 925 174 188 94 21
Cuts at: 2488 3558 4248
Size: 1070 690
Fragments arranged by size:
1070 925 845 690 231 188 174 94
21 10
SphI G_CATG'C
Cuts at: 0 82 388 2893 3790 4248
Size: 82 306 2505 897 458
Fragments arranged by size:
2505 897 458 306 82
SspI AAT'ATT
Cuts at: 0 885 1123 2457 2717 4248
Size: 885 238 1334 260 1531
Fragments arranged by size:
1531 1334 885 260 238
StyI C'CwwG_G
Cuts at: 0 465 4248
Size: 465 3783
SwaI ATTT'AAAT
Cuts at: 0 1119 4248
Size: 1119 3129
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 122 1391 4248
Size: 122 1269 2857
Fragments arranged by size:
2857 1269 122
TatI w'GTAC_w
Cuts at: 0 365 1222 2762 4248
Size: 365 857 1540 1486
Fragments arranged by size:
1540 1486 857 365
XmnI GAAnn'nnTTC
Cuts at: 0 4196 4248
Size: 4196 52
ZraI GAC'GTC
Cuts at: 0 152 4248
Size: 152 4096
Enzymes that do cut:
AatII AccI AcuI AflII AflIII AlfI ApoI BanI
BanII BbeI BbsI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaBI BsaHI
BsaXI BseRI BsgI BsiHKAI BsmI Bsp1286I BspHI BspMI
BsrBI BsrDI BssHII BssSI BstAPI BstYI BstZ17I Bsu36I
BtgI BtsI DraI DraIII EaeI EarI EciI EcoICRI
Eco57MI EcoO109I FalI HaeII Hin4I HincII HindIII HpaI
KasI MfeI MmeI MscI MslI MspA1I NarI NcoI
NdeI NsiI NspI PacI PciI PsrI PstI PvuII
SacI SapI SbfI SfcI SfoI SmlI SphI SspI
StyI SwaI TaqII TatI XmnI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AfeI AgeI AhdI AleI AloI
AlwNI ApaI ApaLI AscI AseI AsiSI AvaI AvrII
BaeI BamHI BbvCI BcgI BciVI BmtI Bpu10I BsaI
BsaAI BsaWI BseYI BsiEI BsiWI BsmBI BspEI BsrFI
BsrGI BstBI BstEII BstXI BtgZI ClaI CspCI DrdI
EagI EcoNI EcoRI EcoRV FseI FspI FspAI KpnI
MluI NaeI NgoMIV NheI NotI NruI PasI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PspXI PvuI RsrII SacII SalI SanDI ScaI SexAI
SfiI SgfI SgrAI SmaI SnaBI SpeI SrfI StuI
TstI Tth111I XbaI XcmI XhoI XmaI