Restriction map for
pF1KA1565
(Linear) (Six-base) MAPSORT of: KIAA1565.seq Check: 824 from: 1 to: 3825
KIAA1565.orf
With 182 enzymes: SgfI *
April 24, 2009 13:00 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2150 3825
Size: 2150 1675
Acc65I G'GTAC_C
Cuts at: 0 3544 3825
Size: 3544 281
AclI AA'CG_TT
Cuts at: 0 2793 3825
Size: 2793 1032
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 145 383 997 1495 1805 2020 2218 2371
Size: 145 238 614 498 310 215 198 153
Cuts at: 2371 2867 3271 3825
Size: 496 404 554
Fragments arranged by size:
614 554 498 496 404 310 238 215
198 153 145
AflIII A'CryG_T
Cuts at: 0 113 3825
Size: 113 3712
AleI CACnn'nnGTG
Cuts at: 0 2818 3825
Size: 2818 1007
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3092 3790 3825
Size: 3092 698 35
Fragments arranged by size:
3092 698 35
AlwNI CAG_nnn'CTG
Cuts at: 0 1332 3408 3805 3825
Size: 1332 2076 397 20
Fragments arranged by size:
2076 1332 397 20
ApaI G_GGCC'C
Cuts at: 0 3315 3825
Size: 3315 510
ApaLI G'TGCA_C
Cuts at: 0 1685 3825
Size: 1685 2140
ApoI r'AATT_y
Cuts at: 0 1518 2427 2577 3165 3645 3825
Size: 1518 909 150 588 480 180
Fragments arranged by size:
1518 909 588 480 180 150
AseI AT'TA_AT
Cuts at: 0 211 3825
Size: 211 3614
AvaI C'yCGr_G
Cuts at: 0 331 670 3476 3825
Size: 331 339 2806 349
Fragments arranged by size:
2806 349 339 331
BanI G'GyrC_C
Cuts at: 0 797 3544 3825
Size: 797 2747 281
Fragments arranged by size:
2747 797 281
BanII G_rGCy'C
Cuts at: 0 1766 1778 1886 2180 2363 2843 3315 3344
Size: 1766 12 108 294 183 480 472 29
Cuts at: 3344 3825
Size: 481
Fragments arranged by size:
1766 481 480 472 294 183 108 29
12
BbsI GAAGACnn'nnnn_
Cuts at: 0 448 873 2816 3179 3825
Size: 448 425 1943 363 646
Fragments arranged by size:
1943 646 448 425 363
BbvCI CC'TCA_GC
Cuts at: 0 2155 3825
Size: 2155 1670
BclI T'GATC_A
Cuts at: 0 1215 3594 3825
Size: 1215 2379 231
Fragments arranged by size:
2379 1215 231
BfrBI ATG'CAT
Cuts at: 0 913 3825
Size: 913 2912
BglII A'GATC_T
Cuts at: 0 1164 3825
Size: 1164 2661
BlpI GC'TnA_GC
Cuts at: 0 281 3825
Size: 281 3544
Bme1580I G_kGCm'C
Cuts at: 0 73 1689 3315 3825
Size: 73 1616 1626 510
Fragments arranged by size:
1626 1616 510 73
BmrI ACTGGGnnnn_n'
Cuts at: 0 133 3825
Size: 133 3692
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 685 3825
Size: 685 3140
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1645 1900 2851 2926 3418 3825
Size: 1645 255 951 75 492 407
Fragments arranged by size:
1645 951 492 407 255 75
Bpu10I CC'TnA_GC
Cuts at: 0 1702 2155 3825
Size: 1702 453 1670
Fragments arranged by size:
1702 1670 453
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 22 1171 2041 2143 2164 2186 2356 3825
Size: 22 1149 870 102 21 22 170 1469
Fragments arranged by size:
1469 1149 870 170 102 22 22 21
BsaI GGTCTCn'nnnn_
Cuts at: 0 627 3825
Size: 627 3198
BsaBI GATnn'nnATC
Cuts at: 0 2403 3825
Size: 2403 1422
BsaHI Gr'CG_yC
Cuts at: 0 3821 3825
Size: 3821 4
BsaWI w'CCGG_w
Cuts at: 0 608 3825
Size: 608 3217
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 142 367 472 2107 2110 2140 2359 3825
Size: 142 225 105 1635 3 30 219 1466
Fragments arranged by size:
1635 1466 225 219 142 105 30 3
BseYI C'CCAG_C
Cuts at: 0 414 3308 3825
Size: 414 2894 517
Fragments arranged by size:
2894 517 414
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1650 2824 2908 3202 3786 3825
Size: 1650 1174 84 294 584 39
Fragments arranged by size:
1650 1174 584 294 84 39
BsiHKAI G_wGCw'C
Cuts at: 0 437 1689 1703 1886 2057 2180 2363 2843
Size: 437 1252 14 183 171 123 183 480
Cuts at: 2843 3825
Size: 982
Fragments arranged by size:
1252 982 480 437 183 183 171 123
14
BsmI GAATG_Cn'
Cuts at: 0 513 1067 1795 3297 3825
Size: 513 554 728 1502 528
Fragments arranged by size:
1502 728 554 528 513
Bsp1286I G_dGCh'C
Cuts at: 0 73 437 1689 1703 1766 1778 1886 2057
Size: 73 364 1252 14 63 12 108 171
Cuts at: 2057 2180 2363 2843 3315 3344 3825
Size: 123 183 480 472 29 481
Fragments arranged by size:
1252 481 480 472 364 183 171 123
108 73 63 29 14 12
BspHI T'CATG_A
Cuts at: 0 2070 3825
Size: 2070 1755
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2150 3203 3825
Size: 2150 1053 622
Fragments arranged by size:
2150 1053 622
BsrBI CCG'CTC
Cuts at: 0 1236 1623 2217 3825
Size: 1236 387 594 1608
Fragments arranged by size:
1608 1236 594 387
BsrDI GCAATG_nn'
Cuts at: 0 860 3825
Size: 860 2965
BsrFI r'CCGG_y
Cuts at: 0 418 3825
Size: 418 3407
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1332 3825
Size: 1332 2493
BstEII G'GTnAC_C
Cuts at: 0 598 1053 1549 3825
Size: 598 455 496 2276
Fragments arranged by size:
2276 598 496 455
BstYI r'GATC_y
Cuts at: 0 523 971 1164 1328 2506 3825
Size: 523 448 193 164 1178 1319
Fragments arranged by size:
1319 1178 523 448 193 164
Bsu36I CC'TnA_GG
Cuts at: 0 1255 3806 3825
Size: 1255 2551 19
Fragments arranged by size:
2551 1255 19
BtgI C'CryG_G
Cuts at: 0 347 1928 3440 3825
Size: 347 1581 1512 385
Fragments arranged by size:
1581 1512 385 347
BtsI GCAGTG_nn'
Cuts at: 0 435 459 1572 1633 2057 3813 3825
Size: 435 24 1113 61 424 1756 12
Fragments arranged by size:
1756 1113 435 424 61 24 12
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2300 2335 3825
Size: 2300 35 1490
Fragments arranged by size:
2300 1490 35
DraI TTT'AAA
Cuts at: 0 201 741 1430 2459 2582 3254 3825
Size: 201 540 689 1029 123 672 571
Fragments arranged by size:
1029 689 672 571 540 201 123
DraIII CAC_nnn'GTG
Cuts at: 0 520 3825
Size: 520 3305
EaeI y'GGCC_r
Cuts at: 0 1085 1226 3825
Size: 1085 141 2599
Fragments arranged by size:
2599 1085 141
EarI CTCTTCn'nnn_
Cuts at: 0 545 941 1265 1388 1583 2249 3825
Size: 545 396 324 123 195 666 1576
Fragments arranged by size:
1576 666 545 396 324 195 123
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1254 2851 3825
Size: 1254 1597 974
Fragments arranged by size:
1597 1254 974
EcoICRI GAG'CTC
Cuts at: 0 1884 2178 2361 2841 3825
Size: 1884 294 183 480 984
Fragments arranged by size:
1884 984 480 294 183
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 145 383 997 1495 1645 1805 1900 2020
Size: 145 238 614 498 150 160 95 120
Cuts at: 2020 2218 2371 2851 2867 2926 3271 3418 3825
Size: 198 153 480 16 59 345 147 407
Fragments arranged by size:
614 498 480 407 345 238 198 160
153 150 147 145 120 95 59 16
EcoNI CCTnn'n_nnAGG
Cuts at: 0 85 1336 3825
Size: 85 1251 2489
Fragments arranged by size:
2489 1251 85
EcoO109I rG'GnC_Cy
Cuts at: 0 77 323 3312 3825
Size: 77 246 2989 513
Fragments arranged by size:
2989 513 246 77
EcoRI G'AATT_C
Cuts at: 0 3645 3825
Size: 3645 180
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2137 3825
Size: 2137 1688
HaeII r_GCGC'y
Cuts at: 0 2246 3825
Size: 2246 1579
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1313 1345 1868 1900 2184 2216 3312 3344
Size: 1313 32 523 32 284 32 1096 32
Cuts at: 3344 3825
Size: 481
Fragments arranged by size:
1313 1096 523 481 284 32 32 32
32
HindIII A'AGCT_T
Cuts at: 0 2119 2208 2413 3825
Size: 2119 89 205 1412
Fragments arranged by size:
2119 1412 205 89
KpnI G_GTAC'C
Cuts at: 0 3548 3825
Size: 3548 277
MfeI C'AATT_G
Cuts at: 0 2620 3493 3825
Size: 2620 873 332
Fragments arranged by size:
2620 873 332
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1097 3359 3726 3825
Size: 1097 2262 367 99
Fragments arranged by size:
2262 1097 367 99
MscI TGG'CCA
Cuts at: 0 1087 3825
Size: 1087 2738
MslI CAynn'nnrTG
Cuts at: 0 2818 3116 3825
Size: 2818 298 709
Fragments arranged by size:
2818 709 298
MspA1I CmG'CkG
Cuts at: 0 1751 1917 1937 2307 2922 3138 3408 3825
Size: 1751 166 20 370 615 216 270 417
Fragments arranged by size:
1751 615 417 370 270 216 166 20
NcoI C'CATG_G
Cuts at: 0 3440 3825
Size: 3440 385
NdeI CA'TA_TG
Cuts at: 0 1864 3825
Size: 1864 1961
NsiI A_TGCA'T
Cuts at: 0 915 3825
Size: 915 2910
NspI r_CATG'y
Cuts at: 0 117 3825
Size: 117 3708
PasI CC'CwG_GG
Cuts at: 0 81 3825
Size: 81 3744
PciI A'CATG_T
Cuts at: 0 113 3825
Size: 113 3712
PflMI CCAn_nnn'nTGG
Cuts at: 0 2622 3441 3825
Size: 2622 819 384
Fragments arranged by size:
2622 819 384
PfoI T'CCnGG_A
Cuts at: 0 1406 3205 3825
Size: 1406 1799 620
Fragments arranged by size:
1799 1406 620
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1760 1792 3825
Size: 1760 32 2033
Fragments arranged by size:
2033 1760 32
PpuMI rG'GwC_Cy
Cuts at: 0 77 323 3825
Size: 77 246 3502
Fragments arranged by size:
3502 246 77
PshAI GACnn'nnGTC
Cuts at: 0 321 3825
Size: 321 3504
PspOMI G'GGCC_C
Cuts at: 0 3311 3825
Size: 3311 514
PspXI vC'TCGA_Gb
Cuts at: 0 670 3825
Size: 670 3155
PstI C_TGCA'G
Cuts at: 0 149 430 1337 2147 2559 3825
Size: 149 281 907 810 412 1266
Fragments arranged by size:
1266 907 810 412 281 149
PvuII CAG'CTG
Cuts at: 0 1917 1937 2307 2922 3138 3408 3825
Size: 1917 20 370 615 216 270 417
Fragments arranged by size:
1917 615 417 370 270 216 20
SacI G_AGCT'C
Cuts at: 0 1886 2180 2363 2843 3825
Size: 1886 294 183 480 982
Fragments arranged by size:
1886 982 480 294 183
SanDI GG'GwC_CC
Cuts at: 0 77 3825
Size: 77 3748
SbfI CC_TGCA'GG
Cuts at: 0 2147 3825
Size: 2147 1678
SfcI C'TryA_G
Cuts at: 0 145 426 1333 2143 2555 3825
Size: 145 281 907 810 412 1270
Fragments arranged by size:
1270 907 810 412 281 145
SmaI CCC'GGG
Cuts at: 0 3478 3825
Size: 3478 347
SmlI C'TyrA_G
Cuts at: 0 37 670 1186 2056 2122 2179 2201 2335
Size: 37 633 516 870 66 57 22 134
Cuts at: 2335 3825
Size: 1490
Fragments arranged by size:
1490 870 633 516 134 66 57 37
22
StuI AGG'CCT
Cuts at: 0 982 2231 3692 3825
Size: 982 1249 1461 133
Fragments arranged by size:
1461 1249 982 133
StyI C'CwwG_G
Cuts at: 0 983 3440 3825
Size: 983 2457 385
Fragments arranged by size:
2457 983 385
TatI w'GTAC_w
Cuts at: 0 1247 1274 3825
Size: 1247 27 2551
Fragments arranged by size:
2551 1247 27
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 576 608 3794 3825
Size: 576 32 3186 31
Fragments arranged by size:
3186 576 32 31
XbaI T'CTAG_A
Cuts at: 0 1461 3825
Size: 1461 2364
XhoI C'TCGA_G
Cuts at: 0 670 3825
Size: 670 3155
XmaI C'CCGG_G
Cuts at: 0 3476 3825
Size: 3476 349
XmnI GAAnn'nnTTC
Cuts at: 0 251 2231 2414 3158 3671 3825
Size: 251 1980 183 744 513 154
Fragments arranged by size:
1980 744 513 251 183 154
Enzymes that do cut:
AarI Acc65I AclI AcuI AflIII AleI AlfI AlwNI
ApaI ApaLI ApoI AseI AvaI BanI BanII BbsI
BbvCI BclI BfrBI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaBI BsaHI BsaWI BseRI
BseYI BsgI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI
BsrDI BsrFI BstAPI BstEII BstYI Bsu36I BtgI BtsI
CspCI DraI DraIII EaeI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI HaeII Hin4I HindIII KpnI
MfeI MmeI MscI MslI MspA1I NcoI NdeI NsiI
NspI PasI PciI PflMI PfoI PpiI PpuMI PshAI
PspOMI PspXI PstI PvuII SacI SanDI SbfI SfcI
SmaI SmlI StuI StyI TatI TstI XbaI XhoI
XmaI XmnI
Enzymes that do not cut:
AatII AccI AfeI AflII AgeI AhdI AloI AscI
AsiSI AvrII BaeI BamHI BbeI BcgI BciVI BglI
BmgBI BmtI BsaAI BsaXI BsiEI BsiWI BsmBI BspEI
BsrGI BssHII BssSI BstBI BstXI BstZ17I BtgZI ClaI
DrdI EagI EcoRV FseI FspI FspAI HincII HpaI
KasI MluI NaeI NarI NgoMIV NheI NotI NruI
PacI PmeI PmlI PsiI PsrI PvuI RsrII SacII
SalI SapI ScaI SexAI SfiI SfoI SgfI SgrAI
SnaBI SpeI SphI SrfI SspI SwaI TaqII Tth111I
XcmI ZraI