Restriction map for
pF1KSDA1572
(Linear) (Six-base) MAPSORT of: KIAA1572.seq Check: 7603 from: 1 to: 1323
KIAA1572.orf
With 182 enzymes: SgfI *
October 24, 2006 15:17 ..
AccI GT'mk_AC
Cuts at: 0 794 900 1323
Size: 794 106 423
Fragments arranged by size:
794 423 106
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 313 527 1323
Size: 313 214 796
Fragments arranged by size:
796 313 214
AflII C'TTAA_G
Cuts at: 0 171 1296 1323
Size: 171 1125 27
Fragments arranged by size:
1125 171 27
AleI CACnn'nnGTG
Cuts at: 0 950 1323
Size: 950 373
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 476 1323
Size: 476 847
AlwNI CAG_nnn'CTG
Cuts at: 0 366 1260 1323
Size: 366 894 63
Fragments arranged by size:
894 366 63
ApaLI G'TGCA_C
Cuts at: 0 354 1323
Size: 354 969
ApoI r'AATT_y
Cuts at: 0 91 280 663 1323
Size: 91 189 383 660
Fragments arranged by size:
660 383 189 91
BanII G_rGCy'C
Cuts at: 0 647 1323
Size: 647 676
BbsI GAAGACnn'nnnn_
Cuts at: 0 515 1323
Size: 515 808
BglII A'GATC_T
Cuts at: 0 15 1323
Size: 15 1308
Bme1580I G_kGCm'C
Cuts at: 0 358 826 1323
Size: 358 468 497
Fragments arranged by size:
497 468 358
BmrI ACTGGGnnnn_n'
Cuts at: 0 432 1323
Size: 432 891
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 449 1323
Size: 449 874
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 874 1323
Size: 874 449
BsaI GGTCTCn'nnnn_
Cuts at: 0 170 1323
Size: 170 1153
BseYI C'CCAG_C
Cuts at: 0 647 1323
Size: 647 676
BsiHKAI G_wGCw'C
Cuts at: 0 358 1323
Size: 358 965
Bsp1286I G_dGCh'C
Cuts at: 0 358 647 826 1323
Size: 358 289 179 497
Fragments arranged by size:
497 358 289 179
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 148 1258 1323
Size: 148 1110 65
Fragments arranged by size:
1110 148 65
BsrGI T'GTAC_A
Cuts at: 0 1167 1204 1323
Size: 1167 37 119
Fragments arranged by size:
1167 119 37
BstYI r'GATC_y
Cuts at: 0 15 1323
Size: 15 1308
BtsI GCAGTG_nn'
Cuts at: 0 623 744 1323
Size: 623 121 579
Fragments arranged by size:
623 579 121
DraI TTT'AAA
Cuts at: 0 875 1323
Size: 875 448
DraIII CAC_nnn'GTG
Cuts at: 0 427 1323
Size: 427 896
EarI CTCTTCn'nnn_
Cuts at: 0 77 1323
Size: 77 1246
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 313 527 874 1323
Size: 313 214 347 449
Fragments arranged by size:
449 347 313 214
EcoO109I rG'GnC_Cy
Cuts at: 0 363 1257 1323
Size: 363 894 66
Fragments arranged by size:
894 363 66
EcoRI G'AATT_C
Cuts at: 0 280 1323
Size: 280 1043
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 952 1035 1323
Size: 952 83 288
Fragments arranged by size:
952 288 83
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 2 34 236 268 906 938 1323
Size: 2 32 202 32 638 32 385
Fragments arranged by size:
638 385 202 32 32 32 2
MfeI C'AATT_G
Cuts at: 0 247 1323
Size: 247 1076
MslI CAynn'nnrTG
Cuts at: 0 716 751 950 952 1323
Size: 716 35 199 2 371
Fragments arranged by size:
716 371 199 35 2
MspA1I CmG'CkG
Cuts at: 0 1125 1323
Size: 1125 198
NspI r_CATG'y
Cuts at: 0 951 961 1205 1323
Size: 951 10 244 118
Fragments arranged by size:
951 244 118 10
PfoI T'CCnGG_A
Cuts at: 0 1259 1323
Size: 1259 64
PpuMI rG'GwC_Cy
Cuts at: 0 1257 1323
Size: 1257 66
PsiI TTA'TAA
Cuts at: 0 379 1323
Size: 379 944
PstI C_TGCA'G
Cuts at: 0 159 1323
Size: 159 1164
PvuII CAG'CTG
Cuts at: 0 1125 1323
Size: 1125 198
ScaI AGT'ACT
Cuts at: 0 1311 1323
Size: 1311 12
SfcI C'TryA_G
Cuts at: 0 155 805 1323
Size: 155 650 518
Fragments arranged by size:
650 518 155
SmlI C'TyrA_G
Cuts at: 0 171 1296 1323
Size: 171 1125 27
Fragments arranged by size:
1125 171 27
SphI G_CATG'C
Cuts at: 0 961 1323
Size: 961 362
SspI AAT'ATT
Cuts at: 0 783 1323
Size: 783 540
StuI AGG'CCT
Cuts at: 0 803 1323
Size: 803 520
TatI w'GTAC_w
Cuts at: 0 1167 1204 1309 1323
Size: 1167 37 105 14
Fragments arranged by size:
1167 105 37 14
Enzymes that do cut:
AccI AcuI AflII AleI AlfI AlwNI ApaLI ApoI
BanII BbsI BglII Bme1580I BmrI BplI BpmI BsaI
BseYI BsiHKAI Bsp1286I BspMI BsrGI BstYI BtsI DraI
DraIII EarI Eco57MI EcoO109I EcoRI FalI Hin4I MfeI
MslI MspA1I NspI PfoI PpuMI PsiI PstI PvuII
ScaI SfcI SmlI SphI SspI StuI TatI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflIII AgeI AhdI
AloI ApaI AscI AseI AsiSI AvaI AvrII BaeI
BamHI BanI BbeI BbvCI BcgI BciVI BclI BfrBI
BglI BlpI BmgBI BmtI Bpu10I BpuEI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BsgI BsiEI BsiWI BsmI
BsmBI BspEI BspHI BsrBI BsrDI BsrFI BssHII BssSI
BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgI BtgZI
ClaI CspCI DrdI EaeI EagI EciI EcoICRI EcoNI
EcoRV FseI FspI FspAI HaeII HincII HindIII HpaI
KasI KpnI MluI MmeI MscI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI NsiI PacI PasI
PciI PflMI PmeI PmlI PpiI PshAI PspOMI PspXI
PsrI PvuI RsrII SacI SacII SalI SanDI SapI
SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SrfI StyI SwaI TaqII TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI