Restriction map for
pF1KSDA1576
(Linear) (Six-base) MAPSORT of: KIAA1576.seq Check: 8846 from: 1 to: 1257
KIAA1576.orf
With 182 enzymes: SgfI *
October 24, 2006 15:17 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 879 1257
Size: 879 378
AccI GT'mk_AC
Cuts at: 0 437 1257
Size: 437 820
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 159 752 849 1257
Size: 159 593 97 408
Fragments arranged by size:
593 408 159 97
AleI CACnn'nnGTG
Cuts at: 0 424 1257
Size: 424 833
ApaLI G'TGCA_C
Cuts at: 0 556 1257
Size: 556 701
AvaI C'yCGr_G
Cuts at: 0 106 190 1257
Size: 106 84 1067
Fragments arranged by size:
1067 106 84
BanI G'GyrC_C
Cuts at: 0 300 1257
Size: 300 957
BanII G_rGCy'C
Cuts at: 0 158 212 1022 1068 1257
Size: 158 54 810 46 189
Fragments arranged by size:
810 189 158 54 46
BbsI GAAGACnn'nnnn_
Cuts at: 0 784 1257
Size: 784 473
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 457 491 1257
Size: 457 34 766
Fragments arranged by size:
766 457 34
BglI GCCn_nnn'nGGC
Cuts at: 0 983 1257
Size: 983 274
BlpI GC'TnA_GC
Cuts at: 0 593 1257
Size: 593 664
Bme1580I G_kGCm'C
Cuts at: 0 560 1257
Size: 560 697
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 866 1090 1257
Size: 866 224 167
Fragments arranged by size:
866 224 167
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 196 1257
Size: 196 1061
BsaI GGTCTCn'nnnn_
Cuts at: 0 362 790 844 1174 1257
Size: 362 428 54 330 83
Fragments arranged by size:
428 362 330 83 54
BsaAI yAC'GTr
Cuts at: 0 705 1257
Size: 705 552
BsaHI Gr'CG_yC
Cuts at: 0 113 1257
Size: 113 1144
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 359 389 1137 1167 1257
Size: 359 30 748 30 90
Fragments arranged by size:
748 359 90 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 43 937 1087 1216 1219 1257
Size: 43 894 150 129 3 38
Fragments arranged by size:
894 150 129 43 38 3
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 402 1257
Size: 402 855
BsiEI CG_ry'CG
Cuts at: 0 1107 1257
Size: 1107 150
BsiHKAI G_wGCw'C
Cuts at: 0 212 554 560 1257
Size: 212 342 6 697
Fragments arranged by size:
697 342 212 6
BsmI GAATG_Cn'
Cuts at: 0 478 1257
Size: 478 779
BsmBI CGTCTCn'nnnn_
Cuts at: 0 25 1257
Size: 25 1232
Bsp1286I G_dGCh'C
Cuts at: 0 158 212 554 560 1022 1068 1257
Size: 158 54 342 6 462 46 189
Fragments arranged by size:
462 342 189 158 54 46 6
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 879 1257
Size: 879 378
BsrBI CCG'CTC
Cuts at: 0 1239 1257
Size: 1239 18
BsrFI r'CCGG_y
Cuts at: 0 69 1257
Size: 69 1188
BssHII G'CGCG_C
Cuts at: 0 126 1257
Size: 126 1131
BssSI C'ACGA_G
Cuts at: 0 553 1213 1257
Size: 553 660 44
Fragments arranged by size:
660 553 44
BstYI r'GATC_y
Cuts at: 0 215 443 1257
Size: 215 228 814
Fragments arranged by size:
814 228 215
Bsu36I CC'TnA_GG
Cuts at: 0 197 1257
Size: 197 1060
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 921 1257
Size: 921 336
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 213 248 1257
Size: 213 35 1009
Fragments arranged by size:
1009 213 35
DraI TTT'AAA
Cuts at: 0 956 1257
Size: 956 301
EaeI y'GGCC_r
Cuts at: 0 2 1163 1257
Size: 2 1161 94
Fragments arranged by size:
1161 94 2
EarI CTCTTCn'nnn_
Cuts at: 0 178 970 1193 1257
Size: 178 792 223 64
Fragments arranged by size:
792 223 178 64
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 40 88 1257
Size: 40 48 1169
Fragments arranged by size:
1169 48 40
EcoICRI GAG'CTC
Cuts at: 0 210 1257
Size: 210 1047
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 159 752 849 866 1090 1257
Size: 159 593 97 17 224 167
Fragments arranged by size:
593 224 167 159 97 17
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 879 1257
Size: 879 378
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 359 391 1137 1169 1257
Size: 359 32 746 32 88
Fragments arranged by size:
746 359 88 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 857 1257
Size: 857 400
MscI TGG'CCA
Cuts at: 0 4 1165 1257
Size: 4 1161 92
Fragments arranged by size:
1161 92 4
MslI CAynn'nnrTG
Cuts at: 0 424 1257
Size: 424 833
MspA1I CmG'CkG
Cuts at: 0 565 597 1095 1257
Size: 565 32 498 162
Fragments arranged by size:
565 498 162 32
NaeI GCC'GGC
Cuts at: 0 71 1257
Size: 71 1186
NgoMIV G'CCGG_C
Cuts at: 0 69 1257
Size: 69 1188
PasI CC'CwG_GG
Cuts at: 0 804 1257
Size: 804 453
PfoI T'CCnGG_A
Cuts at: 0 446 533 1257
Size: 446 87 724
Fragments arranged by size:
724 446 87
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 624 656 1257
Size: 624 32 601
Fragments arranged by size:
624 601 32
PvuII CAG'CTG
Cuts at: 0 565 597 1257
Size: 565 32 660
Fragments arranged by size:
660 565 32
SacI G_AGCT'C
Cuts at: 0 212 1257
Size: 212 1045
SapI GCTCTTCn'nnn_
Cuts at: 0 178 970 1257
Size: 178 792 287
Fragments arranged by size:
792 287 178
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 983 1257
Size: 983 274
SmlI C'TyrA_G
Cuts at: 0 211 1257
Size: 211 1046
SspI AAT'ATT
Cuts at: 0 273 1257
Size: 273 984
StyI C'CwwG_G
Cuts at: 0 5 1257
Size: 5 1252
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1121 1257
Size: 1121 136
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1045 1077 1257
Size: 1045 32 180
Fragments arranged by size:
1045 180 32
Tth111I GACn'n_nGTC
Cuts at: 0 373 1257
Size: 373 884
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 12 294 1257
Size: 12 282 963
Fragments arranged by size:
963 282 12
Enzymes that do cut:
AarI AccI AcuI AleI ApaLI AvaI BanI BanII
BbsI BcgI BglI BlpI Bme1580I BpmI BpuEI BsaI
BsaAI BsaHI BsaXI BseRI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspMI BsrBI BsrFI BssHII BssSI BstYI
Bsu36I BtgZI CspCI DraI EaeI EarI EciI EcoICRI
Eco57MI FalI Hin4I MmeI MscI MslI MspA1I NaeI
NgoMIV PasI PfoI PsrI PvuII SacI SapI SfiI
SmlI SspI StyI TaqII TstI Tth111I XcmI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI
AlfI AloI AlwNI ApaI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BbeI BbvCI BciVI BclI BfrBI
BglII BmgBI BmrI BmtI BplI Bpu10I BsaBI BsaWI
BseYI BsiWI BspEI BspHI BsrDI BsrGI BstAPI BstBI
BstEII BstXI BstZ17I BtgI BtsI ClaI DraIII DrdI
EagI EcoNI EcoO109I EcoRI EcoRV FseI FspI FspAI
HaeII HincII HindIII HpaI KasI KpnI MfeI MluI
NarI NcoI NdeI NheI NotI NruI NsiI NspI
PacI PciI PflMI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PspXI PstI PvuI RsrII SacII SalI
SanDI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI StuI SwaI TatI
XbaI XhoI XmaI XmnI ZraI