Restriction map for
pF1KSDA1580
(Linear) (Six-base) MAPSORT of: KIAA1580.seq Check: 834 from: 1 to: 1920
KIAA1580.orf
With 182 enzymes: SgfI *
October 24, 2006 15:17 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1760 1920
Size: 1760 160
AccI GT'mk_AC
Cuts at: 0 434 1445 1498 1920
Size: 434 1011 53 422
Fragments arranged by size:
1011 434 422 53
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 469 781 1118 1295 1920
Size: 469 312 337 177 625
Fragments arranged by size:
625 469 337 312 177
AflIII A'CryG_T
Cuts at: 0 1273 1920
Size: 1273 647
AleI CACnn'nnGTG
Cuts at: 0 1438 1462 1920
Size: 1438 24 458
Fragments arranged by size:
1438 458 24
AlwNI CAG_nnn'CTG
Cuts at: 0 1528 1920
Size: 1528 392
ApaI G_GGCC'C
Cuts at: 0 63 1920
Size: 63 1857
ApoI r'AATT_y
Cuts at: 0 509 1233 1842 1920
Size: 509 724 609 78
Fragments arranged by size:
724 609 509 78
AseI AT'TA_AT
Cuts at: 0 1706 1920
Size: 1706 214
AvrII C'CTAG_G
Cuts at: 0 46 1920
Size: 46 1874
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1133 1166 1920
Size: 1133 33 754
Fragments arranged by size:
1133 754 33
BamHI G'GATC_C
Cuts at: 0 1544 1920
Size: 1544 376
BanI G'GyrC_C
Cuts at: 0 571 1920
Size: 571 1349
BanII G_rGCy'C
Cuts at: 0 63 132 458 1031 1076 1920
Size: 63 69 326 573 45 844
Fragments arranged by size:
844 573 326 69 63 45
BbsI GAAGACnn'nnnn_
Cuts at: 0 1576 1920
Size: 1576 344
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1645 1679 1920
Size: 1645 34 241
Fragments arranged by size:
1645 241 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 878 1920
Size: 878 1042
BlpI GC'TnA_GC
Cuts at: 0 934 1112 1920
Size: 934 178 808
Fragments arranged by size:
934 808 178
Bme1580I G_kGCm'C
Cuts at: 0 63 984 1920
Size: 63 921 936
Fragments arranged by size:
936 921 63
BmrI ACTGGGnnnn_n'
Cuts at: 0 1458 1516 1920
Size: 1458 58 404
Fragments arranged by size:
1458 404 58
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1421 1920
Size: 1421 499
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 315 615 1920
Size: 315 300 1305
Fragments arranged by size:
1305 315 300
BsaI GGTCTCn'nnnn_
Cuts at: 0 1447 1920
Size: 1447 473
BsaWI w'CCGG_w
Cuts at: 0 210 1059 1920
Size: 210 849 861
Fragments arranged by size:
861 849 210
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 416 445 446 475 1329 1359 1920
Size: 416 29 1 29 854 30 561
Fragments arranged by size:
854 561 416 30 29 29 1
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 986 1920
Size: 986 934
BsiHKAI G_wGCw'C
Cuts at: 0 154 458 1031 1217 1492 1781 1920
Size: 154 304 573 186 275 289 139
Fragments arranged by size:
573 304 289 275 186 154 139
BsmI GAATG_Cn'
Cuts at: 0 786 1920
Size: 786 1134
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1397 1920
Size: 1397 523
Bsp1286I G_dGCh'C
Cuts at: 0 63 132 154 458 984 1031 1076 1217
Size: 63 69 22 304 526 47 45 141
Cuts at: 1217 1492 1781 1920
Size: 275 289 139
Fragments arranged by size:
526 304 289 275 141 139 69 63
47 45 22
BspEI T'CCGG_A
Cuts at: 0 210 1920
Size: 210 1710
BspHI T'CATG_A
Cuts at: 0 849 1178 1565 1920
Size: 849 329 387 355
Fragments arranged by size:
849 387 355 329
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 145 206 1760 1920
Size: 145 61 1554 160
Fragments arranged by size:
1554 160 145 61
BsrBI CCG'CTC
Cuts at: 0 651 882 1920
Size: 651 231 1038
Fragments arranged by size:
1038 651 231
BsrGI T'GTAC_A
Cuts at: 0 1268 1902 1920
Size: 1268 634 18
Fragments arranged by size:
1268 634 18
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1440 1920
Size: 1440 480
BstYI r'GATC_y
Cuts at: 0 672 1544 1920
Size: 672 872 376
Fragments arranged by size:
872 672 376
BstZ17I GTA'TAC
Cuts at: 0 435 1920
Size: 435 1485
BtgI C'CryG_G
Cuts at: 0 1739 1920
Size: 1739 181
BtsI GCAGTG_nn'
Cuts at: 0 1628 1920
Size: 1628 292
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 724 759 1920
Size: 724 35 1161
Fragments arranged by size:
1161 724 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 1090 1920
Size: 1090 830
EaeI y'GGCC_r
Cuts at: 0 1601 1920
Size: 1601 319
EarI CTCTTCn'nnn_
Cuts at: 0 101 862 1920
Size: 101 761 1058
Fragments arranged by size:
1058 761 101
EcoICRI GAG'CTC
Cuts at: 0 456 1029 1920
Size: 456 573 891
Fragments arranged by size:
891 573 456
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 469 781 1118 1295 1421 1920
Size: 469 312 337 177 126 499
Fragments arranged by size:
499 469 337 312 177 126
EcoNI CCTnn'n_nnAGG
Cuts at: 0 202 1920
Size: 202 1718
EcoO109I rG'GnC_Cy
Cuts at: 0 43 59 60 1429 1920
Size: 43 16 1 1369 491
Fragments arranged by size:
1369 491 43 16 1
EcoRI G'AATT_C
Cuts at: 0 509 1920
Size: 509 1411
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 33 506 1920
Size: 33 473 1414
Fragments arranged by size:
1414 473 33
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 6 38 205 237 1458 1490 1920
Size: 6 32 167 32 1221 32 430
Fragments arranged by size:
1221 430 167 32 32 32 6
HincII GTy'rAC
Cuts at: 0 1446 1499 1833 1920
Size: 1446 53 334 87
Fragments arranged by size:
1446 334 87 53
HpaI GTT'AAC
Cuts at: 0 1833 1920
Size: 1833 87
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 614 1920
Size: 614 1306
MscI TGG'CCA
Cuts at: 0 1603 1920
Size: 1603 317
MslI CAynn'nnrTG
Cuts at: 0 274 1276 1438 1462 1522 1612 1738 1920
Size: 274 1002 162 24 60 90 126 182
Fragments arranged by size:
1002 274 182 162 126 90 60 24
MspA1I CmG'CkG
Cuts at: 0 938 1111 1920
Size: 938 173 809
Fragments arranged by size:
938 809 173
NcoI C'CATG_G
Cuts at: 0 1739 1920
Size: 1739 181
NspI r_CATG'y
Cuts at: 0 1052 1269 1277 1920
Size: 1052 217 8 643
Fragments arranged by size:
1052 643 217 8
PciI A'CATG_T
Cuts at: 0 1273 1920
Size: 1273 647
PpuMI rG'GwC_Cy
Cuts at: 0 43 1429 1920
Size: 43 1386 491
Fragments arranged by size:
1386 491 43
PshAI GACnn'nnGTC
Cuts at: 0 401 1920
Size: 401 1519
PspOMI G'GGCC_C
Cuts at: 0 59 1920
Size: 59 1861
PstI C_TGCA'G
Cuts at: 0 160 1083 1335 1623 1920
Size: 160 923 252 288 297
Fragments arranged by size:
923 297 288 252 160
PvuII CAG'CTG
Cuts at: 0 938 1111 1920
Size: 938 173 809
Fragments arranged by size:
938 809 173
SacI G_AGCT'C
Cuts at: 0 458 1031 1920
Size: 458 573 889
Fragments arranged by size:
889 573 458
SalI G'TCGA_C
Cuts at: 0 1444 1497 1920
Size: 1444 53 423
Fragments arranged by size:
1444 423 53
SanDI GG'GwC_CC
Cuts at: 0 1429 1920
Size: 1429 491
SapI GCTCTTCn'nnn_
Cuts at: 0 101 1920
Size: 101 1819
SfcI C'TryA_G
Cuts at: 0 156 313 1079 1331 1619 1920
Size: 156 157 766 252 288 301
Fragments arranged by size:
766 301 288 252 157 156
SmlI C'TyrA_G
Cuts at: 0 294 594 1920
Size: 294 300 1326
Fragments arranged by size:
1326 300 294
SpeI A'CTAG_T
Cuts at: 0 804 1920
Size: 804 1116
StuI AGG'CCT
Cuts at: 0 705 1920
Size: 705 1215
StyI C'CwwG_G
Cuts at: 0 46 904 1739 1920
Size: 46 858 835 181
Fragments arranged by size:
858 835 181 46
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1580 1752 1920
Size: 1580 172 168
Fragments arranged by size:
1580 172 168
TatI w'GTAC_w
Cuts at: 0 1268 1902 1920
Size: 1268 634 18
Fragments arranged by size:
1268 634 18
XbaI T'CTAG_A
Cuts at: 0 876 1920
Size: 876 1044
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1445 1611 1920
Size: 1445 166 309
Fragments arranged by size:
1445 309 166
XmnI GAAnn'nnTTC
Cuts at: 0 283 513 1042 1510 1920
Size: 283 230 529 468 410
Fragments arranged by size:
529 468 410 283 230
Enzymes that do cut:
AarI AccI AcuI AflIII AleI AlwNI ApaI ApoI
AseI AvrII BaeI BamHI BanI BanII BbsI BcgI
BciVI BlpI Bme1580I BmrI BpmI BpuEI BsaI BsaWI
BsaXI BseRI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrGI BstXI BstYI BstZ17I BtgI BtsI
CspCI DrdI EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI Hin4I HincII HpaI MmeI MscI MslI
MspA1I NcoI NspI PciI PpuMI PshAI PspOMI PstI
PvuII SacI SalI SanDI SapI SfcI SmlI SpeI
StuI StyI TaqII TatI XbaI XcmI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AhdI AlfI
AloI ApaLI AscI AsiSI AvaI BbeI BbvCI BclI
BfrBI BglI BglII BmgBI BmtI BplI Bpu10I BsaAI
BsaBI BsaHI BseYI BsgI BsiEI BsiWI BsrDI BsrFI
BssHII BssSI BstAPI BstBI BstEII Bsu36I BtgZI ClaI
DraI DraIII EagI EciI EcoRV FseI FspI FspAI
HaeII HindIII KasI KpnI MfeI MluI NaeI NarI
NdeI NgoMIV NheI NotI NruI NsiI PacI PasI
PflMI PfoI PmeI PmlI PpiI PsiI PspXI PsrI
PvuI RsrII SacII SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SphI SrfI SspI SwaI
TstI Tth111I XhoI XmaI ZraI