Restriction map for
pF1KSDA1581
(Linear) (Six-base) MAPSORT of: KIAA1581.seq Check: 7069 from: 1 to: 1476
KIAA1581.orf
With 182 enzymes: SgfI *
October 24, 2006 15:17 ..
AccI GT'mk_AC
Cuts at: 0 1352 1476
Size: 1352 124
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1201 1476
Size: 1201 275
AflIII A'CryG_T
Cuts at: 0 1040 1476
Size: 1040 436
ApaLI G'TGCA_C
Cuts at: 0 1267 1476
Size: 1267 209
ApoI r'AATT_y
Cuts at: 0 7 1476
Size: 7 1469
AvaI C'yCGr_G
Cuts at: 0 788 1476
Size: 788 688
BanI G'GyrC_C
Cuts at: 0 51 1476
Size: 51 1425
BanII G_rGCy'C
Cuts at: 0 566 1294 1476
Size: 566 728 182
Fragments arranged by size:
728 566 182
BbsI GAAGACnn'nnnn_
Cuts at: 0 1409 1476
Size: 1409 67
BciVI GTATCCnnnnn_n'
Cuts at: 0 322 908 1476
Size: 322 586 568
Fragments arranged by size:
586 568 322
BglI GCCn_nnn'nGGC
Cuts at: 0 820 1476
Size: 820 656
BlpI GC'TnA_GC
Cuts at: 0 712 1476
Size: 712 764
Bme1580I G_kGCm'C
Cuts at: 0 56 1271 1476
Size: 56 1215 205
Fragments arranged by size:
1215 205 56
BmrI ACTGGGnnnn_n'
Cuts at: 0 1126 1476
Size: 1126 350
Bpu10I CC'TnA_GC
Cuts at: 0 512 1476
Size: 512 964
BsaBI GATnn'nnATC
Cuts at: 0 1063 1476
Size: 1063 413
BsaWI w'CCGG_w
Cuts at: 0 41 1355 1476
Size: 41 1314 121
Fragments arranged by size:
1314 121 41
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1369 1399 1476
Size: 1369 30 77
Fragments arranged by size:
1369 77 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1450 1476
Size: 1450 26
BseYI C'CCAG_C
Cuts at: 0 681 922 1204 1476
Size: 681 241 282 272
Fragments arranged by size:
681 282 272 241
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 334 597 1476
Size: 334 263 879
Fragments arranged by size:
879 334 263
BsiHKAI G_wGCw'C
Cuts at: 0 943 1271 1476
Size: 943 328 205
Fragments arranged by size:
943 328 205
BsmI GAATG_Cn'
Cuts at: 0 449 559 1476
Size: 449 110 917
Fragments arranged by size:
917 449 110
Bsp1286I G_dGCh'C
Cuts at: 0 56 566 943 1271 1294 1476
Size: 56 510 377 328 23 182
Fragments arranged by size:
510 377 328 182 56 23
BspHI T'CATG_A
Cuts at: 0 173 1476
Size: 173 1303
BsrDI GCAATG_nn'
Cuts at: 0 110 434 1476
Size: 110 324 1042
Fragments arranged by size:
1042 324 110
BsrGI T'GTAC_A
Cuts at: 0 590 1476
Size: 590 886
BssSI C'ACGA_G
Cuts at: 0 942 1243 1476
Size: 942 301 233
Fragments arranged by size:
942 301 233
BstXI CCAn_nnnn'nTGG
Cuts at: 0 872 1245 1476
Size: 872 373 231
Fragments arranged by size:
872 373 231
BstYI r'GATC_y
Cuts at: 0 1115 1476
Size: 1115 361
BtgI C'CryG_G
Cuts at: 0 1325 1391 1476
Size: 1325 66 85
Fragments arranged by size:
1325 85 66
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 270 1218 1320 1476
Size: 270 948 102 156
Fragments arranged by size:
948 270 156 102
BtsI GCAGTG_nn'
Cuts at: 0 830 1476
Size: 830 646
DraIII CAC_nnn'GTG
Cuts at: 0 357 623 1166 1476
Size: 357 266 543 310
Fragments arranged by size:
543 357 310 266
EaeI y'GGCC_r
Cuts at: 0 146 743 1388 1476
Size: 146 597 645 88
Fragments arranged by size:
645 597 146 88
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1201 1476
Size: 1201 275
EcoO109I rG'GnC_Cy
Cuts at: 0 190 389 792 1476
Size: 190 199 403 684
Fragments arranged by size:
684 403 199 190
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 999 1206 1476
Size: 999 207 270
Fragments arranged by size:
999 270 207
HaeII r_GCGC'y
Cuts at: 0 19 1476
Size: 19 1457
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 744 776 1140 1172 1369 1401 1476
Size: 744 32 364 32 197 32 75
Fragments arranged by size:
744 364 197 75 32 32 32
HincII GTy'rAC
Cuts at: 0 774 878 1476
Size: 774 104 598
Fragments arranged by size:
774 598 104
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1453 1476
Size: 1453 23
MscI TGG'CCA
Cuts at: 0 148 745 1476
Size: 148 597 731
Fragments arranged by size:
731 597 148
MslI CAynn'nnrTG
Cuts at: 0 1072 1476
Size: 1072 404
NcoI C'CATG_G
Cuts at: 0 1325 1476
Size: 1325 151
NspI r_CATG'y
Cuts at: 0 864 1425 1476
Size: 864 561 51
Fragments arranged by size:
864 561 51
PasI CC'CwG_GG
Cuts at: 0 57 1281 1476
Size: 57 1224 195
Fragments arranged by size:
1224 195 57
PpuMI rG'GwC_Cy
Cuts at: 0 190 792 1476
Size: 190 602 684
Fragments arranged by size:
684 602 190
PstI C_TGCA'G
Cuts at: 0 813 1476
Size: 813 663
SanDI GG'GwC_CC
Cuts at: 0 190 1476
Size: 190 1286
SfcI C'TryA_G
Cuts at: 0 809 1476
Size: 809 667
SmaI CCC'GGG
Cuts at: 0 790 1476
Size: 790 686
StuI AGG'CCT
Cuts at: 0 97 1476
Size: 97 1379
StyI C'CwwG_G
Cuts at: 0 795 1325 1476
Size: 795 530 151
Fragments arranged by size:
795 530 151
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1050 1476
Size: 1050 426
TatI w'GTAC_w
Cuts at: 0 590 1476
Size: 590 886
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 156 624 822 849 930 1476
Size: 156 468 198 27 81 546
Fragments arranged by size:
546 468 198 156 81 27
XmaI C'CCGG_G
Cuts at: 0 788 1476
Size: 788 688
XmnI GAAnn'nnTTC
Cuts at: 0 181 1189 1476
Size: 181 1008 287
Fragments arranged by size:
1008 287 181
Enzymes that do cut:
AccI AcuI AflIII ApaLI ApoI AvaI BanI BanII
BbsI BciVI BglI BlpI Bme1580I BmrI Bpu10I BsaBI
BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I
BspHI BsrDI BsrGI BssSI BstXI BstYI BtgI BtgZI
BtsI DraIII EaeI Eco57MI EcoO109I FalI HaeII Hin4I
HincII MmeI MscI MslI NcoI NspI PasI PpuMI
PstI SanDI SfcI SmaI StuI StyI TaqII TatI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AleI AlfI AloI AlwNI ApaI AscI AseI AsiSI
AvrII BaeI BamHI BbeI BbvCI BcgI BclI BfrBI
BglII BmgBI BmtI BplI BpmI BpuEI BsaI BsaAI
BsaHI BsiEI BsiWI BsmBI BspEI BspMI BsrBI BsrFI
BssHII BstAPI BstBI BstEII BstZ17I Bsu36I ClaI CspCI
DraI DrdI EagI EarI EciI EcoICRI EcoNI EcoRI
EcoRV FseI FspI FspAI HindIII HpaI KasI KpnI
MfeI MluI MspA1I NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI PacI PciI PflMI PfoI PmeI
PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI
PvuII RsrII SacI SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI
SphI SrfI SspI SwaI TstI Tth111I XbaI XhoI
ZraI