Restriction map for
pF1KA1582
(Linear) (Six-base) MAPSORT of: KIAA1582.seq Check: 3300 from: 1 to: 5070
KIAA1582.orf
With 182 enzymes: SgfI *
October 24, 2006 15:08 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 671 1548 5070
Size: 671 877 3522
Fragments arranged by size:
3522 877 671
AatII G_ACGT'C
Cuts at: 0 2791 4226 5070
Size: 2791 1435 844
Fragments arranged by size:
2791 1435 844
Acc65I G'GTAC_C
Cuts at: 0 2088 2179 5070
Size: 2088 91 2891
Fragments arranged by size:
2891 2088 91
AclI AA'CG_TT
Cuts at: 0 3486 5070
Size: 3486 1584
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 743 1496 3136 5070
Size: 743 753 1640 1934
Fragments arranged by size:
1934 1640 753 743
AfeI AGC'GCT
Cuts at: 0 2919 5070
Size: 2919 2151
AflIII A'CryG_T
Cuts at: 0 2228 2696 4709 5070
Size: 2228 468 2013 361
Fragments arranged by size:
2228 2013 468 361
AgeI A'CCGG_T
Cuts at: 0 2091 3844 5070
Size: 2091 1753 1226
Fragments arranged by size:
2091 1753 1226
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 877 1388 3578 3701 4951 5070
Size: 877 511 2190 123 1250 119
Fragments arranged by size:
2190 1250 877 511 123 119
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1468 1500 5070
Size: 1468 32 3570
Fragments arranged by size:
3570 1468 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1519 1882 1919 2744 3156 3627 4541 4794
Size: 1519 363 37 825 412 471 914 253
Cuts at: 4794 5070
Size: 276
Fragments arranged by size:
1519 914 825 471 412 363 276 253
37
ApaI G_GGCC'C
Cuts at: 0 4981 5070
Size: 4981 89
ApaLI G'TGCA_C
Cuts at: 0 3137 5070
Size: 3137 1933
ApoI r'AATT_y
Cuts at: 0 1381 3195 4162 5070
Size: 1381 1814 967 908
Fragments arranged by size:
1814 1381 967 908
AseI AT'TA_AT
Cuts at: 0 287 3428 5070
Size: 287 3141 1642
Fragments arranged by size:
3141 1642 287
AvaI C'yCGr_G
Cuts at: 0 1599 2856 4497 5070
Size: 1599 1257 1641 573
Fragments arranged by size:
1641 1599 1257 573
AvrII C'CTAG_G
Cuts at: 0 1396 5070
Size: 1396 3674
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 2649 2682 5070
Size: 2649 33 2388
Fragments arranged by size:
2649 2388 33
BamHI G'GATC_C
Cuts at: 0 847 3535 5070
Size: 847 2688 1535
Fragments arranged by size:
2688 1535 847
BanI G'GyrC_C
Cuts at: 0 64 2088 2179 4389 4462 4950 5070
Size: 64 2024 91 2210 73 488 120
Fragments arranged by size:
2210 2024 488 120 91 73 64
BanII G_rGCy'C
Cuts at: 0 3083 4981 5070
Size: 3083 1898 89
Fragments arranged by size:
3083 1898 89
BbsI GAAGACnn'nnnn_
Cuts at: 0 52 2791 3020 3761 5070
Size: 52 2739 229 741 1309
Fragments arranged by size:
2739 1309 741 229 52
BbvCI CC'TCA_GC
Cuts at: 0 2184 2592 2870 5070
Size: 2184 408 278 2200
Fragments arranged by size:
2200 2184 408 278
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1787 1821 3677 3711 4527 4561 5070
Size: 1787 34 1856 34 816 34 509
Fragments arranged by size:
1856 1787 816 509 34 34 34
BclI T'GATC_A
Cuts at: 0 2828 2904 5070
Size: 2828 76 2166
Fragments arranged by size:
2828 2166 76
BglI GCCn_nnn'nGGC
Cuts at: 0 1162 4475 4916 4976 5070
Size: 1162 3313 441 60 94
Fragments arranged by size:
3313 1162 441 94 60
BglII A'GATC_T
Cuts at: 0 1903 5070
Size: 1903 3167
BlpI GC'TnA_GC
Cuts at: 0 4846 5053 5070
Size: 4846 207 17
Fragments arranged by size:
4846 207 17
Bme1580I G_kGCm'C
Cuts at: 0 18 108 1292 3141 4394 4955 4981 5070
Size: 18 90 1184 1849 1253 561 26 89
Fragments arranged by size:
1849 1253 1184 561 90 89 26 18
BmgBI CAC'GTC
Cuts at: 0 2697 2943 4331 5070
Size: 2697 246 1388 739
Fragments arranged by size:
2697 1388 739 246
BmrI ACTGGGnnnn_n'
Cuts at: 0 630 1241 1752 1971 2007 2043 3140 3311
Size: 630 611 511 219 36 36 1097 171
Cuts at: 3311 3867 3873 3888 3923 4256 4950 5070
Size: 556 6 15 35 333 694 120
Fragments arranged by size:
1097 694 630 611 556 511 333 219
171 120 36 36 35 15 6
BmtI G_CTAG'C
Cuts at: 0 2006 5070
Size: 2006 3064
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 4439 5070
Size: 4439 631
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 156 1794 3967 3986 4001 4409 4530 4947
Size: 156 1638 2173 19 15 408 121 417
Cuts at: 4947 5070
Size: 123
Fragments arranged by size:
2173 1638 417 408 156 123 121 19
15
Bpu10I CC'TnA_GC
Cuts at: 0 2184 2592 2870 5070
Size: 2184 408 278 2200
Fragments arranged by size:
2200 2184 408 278
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 686 3314 4279 4628 5070
Size: 686 2628 965 349 442
Fragments arranged by size:
2628 965 686 442 349
BsaI GGTCTCn'nnnn_
Cuts at: 0 3403 5070
Size: 3403 1667
BsaAI yAC'GTr
Cuts at: 0 4105 5070
Size: 4105 965
BsaHI Gr'CG_yC
Cuts at: 0 2329 2788 4223 5070
Size: 2329 459 1435 847
Fragments arranged by size:
2329 1435 847 459
BsaWI w'CCGG_w
Cuts at: 0 2091 3532 3844 4661 5070
Size: 2091 1441 312 817 409
Fragments arranged by size:
2091 1441 817 409 312
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 800 830 1132 1162 1684 1714 3771 3801
Size: 800 30 302 30 522 30 2057 30
Cuts at: 3801 3895 3925 5070
Size: 94 30 1145
Fragments arranged by size:
2057 1145 800 522 302 94 30 30
30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 2054 2721 2887 4282 4440 4485 4693 5070
Size: 2054 667 166 1395 158 45 208 377
Fragments arranged by size:
2054 1395 667 377 208 166 158 45
BseYI C'CCAG_C
Cuts at: 0 257 315 991 1919 2126 2405 2512 2633
Size: 257 58 676 928 207 279 107 121
Cuts at: 2633 2958 3177 3311 3518 4984 5070
Size: 325 219 134 207 1466 86
Fragments arranged by size:
1466 928 676 325 279 257 219 207
207 134 121 107 86 58
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1463 1799 3313 3392 3661 4391 4690 5070
Size: 1463 336 1514 79 269 730 299 380
Fragments arranged by size:
1514 1463 730 380 336 299 269 79
BsiHKAI G_wGCw'C
Cuts at: 0 478 3141 3330 4516 5070
Size: 478 2663 189 1186 554
Fragments arranged by size:
2663 1186 554 478 189
BsmI GAATG_Cn'
Cuts at: 0 1047 5070
Size: 1047 4023
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2320 5070
Size: 2320 2750
Bsp1286I G_dGCh'C
Cuts at: 0 18 108 478 1292 3083 3141 3330 4394
Size: 18 90 370 814 1791 58 189 1064
Cuts at: 4394 4516 4955 4981 5070
Size: 122 439 26 89
Fragments arranged by size:
1791 1064 814 439 370 189 122 90
89 58 26 18
BspEI T'CCGG_A
Cuts at: 0 3532 5070
Size: 3532 1538
BspHI T'CATG_A
Cuts at: 0 1490 2816 4374 5070
Size: 1490 1326 1558 696
Fragments arranged by size:
1558 1490 1326 696
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 671 1548 3755 5057 5070
Size: 671 877 2207 1302 13
Fragments arranged by size:
2207 1302 877 671 13
BsrDI GCAATG_nn'
Cuts at: 0 602 4656 5070
Size: 602 4054 414
Fragments arranged by size:
4054 602 414
BsrFI r'CCGG_y
Cuts at: 0 2085 2091 2553 2822 3844 4034 5070
Size: 2085 6 462 269 1022 190 1036
Fragments arranged by size:
2085 1036 1022 462 269 190 6
BssHII G'CGCG_C
Cuts at: 0 3565 5070
Size: 3565 1505
BssSI C'ACGA_G
Cuts at: 0 1899 2301 3070 3631 4413 5070
Size: 1899 402 769 561 782 657
Fragments arranged by size:
1899 782 769 657 561 402
BstAPI GCAn_nnn'nTGC
Cuts at: 0 832 2584 5070
Size: 832 1752 2486
Fragments arranged by size:
2486 1752 832
BstBI TT'CG_AA
Cuts at: 0 4554 5070
Size: 4554 516
BstEII G'GTnAC_C
Cuts at: 0 760 5070
Size: 760 4310
BstXI CCAn_nnnn'nTGG
Cuts at: 0 323 3915 4576 5070
Size: 323 3592 661 494
Fragments arranged by size:
3592 661 494 323
BstYI r'GATC_y
Cuts at: 0 847 1846 1903 3535 3593 5070
Size: 847 999 57 1632 58 1477
Fragments arranged by size:
1632 1477 999 847 58 57
Bsu36I CC'TnA_GG
Cuts at: 0 3353 4524 5070
Size: 3353 1171 546
Fragments arranged by size:
3353 1171 546
BtgI C'CryG_G
Cuts at: 0 350 3032 3701 3914 4180 4840 4866 5070
Size: 350 2682 669 213 266 660 26 204
Fragments arranged by size:
2682 669 660 350 266 213 204 26
BtsI GCAGTG_nn'
Cuts at: 0 189 723 861 948 1370 2118 2523 2646
Size: 189 534 138 87 422 748 405 123
Cuts at: 2646 4242 4585 4935 5070
Size: 1596 343 350 135
Fragments arranged by size:
1596 748 534 422 405 350 343 189
138 135 123 87
ClaI AT'CG_AT
Cuts at: 0 2364 5070
Size: 2364 2706
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 3033 3068 5070
Size: 3033 35 2002
Fragments arranged by size:
3033 2002 35
DraI TTT'AAA
Cuts at: 0 1976 5070
Size: 1976 3094
DraIII CAC_nnn'GTG
Cuts at: 0 3136 5070
Size: 3136 1934
DrdI GACnn_nn'nnGTC
Cuts at: 0 4222 5070
Size: 4222 848
EaeI y'GGCC_r
Cuts at: 0 143 1662 2984 3698 4032 4739 4890 5070
Size: 143 1519 1322 714 334 707 151 180
Fragments arranged by size:
1519 1322 714 707 334 180 151 143
EarI CTCTTCn'nnn_
Cuts at: 0 579 1515 2562 2878 3071 3312 5070
Size: 579 936 1047 316 193 241 1758
Fragments arranged by size:
1758 1047 936 579 316 241 193
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 156 743 1496 1794 3136 3967 3986 4001
Size: 156 587 753 298 1342 831 19 15
Cuts at: 4001 4409 4530 4947 5070
Size: 408 121 417 123
Fragments arranged by size:
1342 831 753 587 417 408 298 156
123 121 19 15
EcoNI CCTnn'n_nnAGG
Cuts at: 0 770 933 5070
Size: 770 163 4137
Fragments arranged by size:
4137 770 163
EcoO109I rG'GnC_Cy
Cuts at: 0 901 2196 3153 3624 3864 4977 5046 5070
Size: 901 1295 957 471 240 1113 69 24
Fragments arranged by size:
1295 1113 957 901 471 240 69 24
EcoRI G'AATT_C
Cuts at: 0 3195 4162 5070
Size: 3195 967 908
Fragments arranged by size:
3195 967 908
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 3385 5070
Size: 3385 1685
HaeII r_GCGC'y
Cuts at: 0 1591 2921 3612 4795 4991 5070
Size: 1591 1330 691 1183 196 79
Fragments arranged by size:
1591 1330 1183 691 196 79
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1684 1716 1792 1824 2167 2199 3207 3239
Size: 1684 32 76 32 343 32 1008 32
Cuts at: 3239 3751 3783 4114 4146 4309 4341 5070
Size: 512 32 331 32 163 32 729
Fragments arranged by size:
1684 1008 729 512 343 331 163 76
32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 1530 3828 4281 4433 5070
Size: 1530 2298 453 152 637
Fragments arranged by size:
2298 1530 637 453 152
HpaI GTT'AAC
Cuts at: 0 4433 5070
Size: 4433 637
KpnI G_GTAC'C
Cuts at: 0 2092 2183 5070
Size: 2092 91 2887
Fragments arranged by size:
2887 2092 91
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 293 1076 1867 5070
Size: 293 783 791 3203
Fragments arranged by size:
3203 791 783 293
MscI TGG'CCA
Cuts at: 0 1664 4892 5070
Size: 1664 3228 178
Fragments arranged by size:
3228 1664 178
MslI CAynn'nnrTG
Cuts at: 0 2845 3838 5070
Size: 2845 993 1232
Fragments arranged by size:
2845 1232 993
MspA1I CmG'CkG
Cuts at: 0 319 1546 2358 2525 2869 3468 3474 3615
Size: 319 1227 812 167 344 599 6 141
Cuts at: 3615 4541 4811 4842 4855 5057 5070
Size: 926 270 31 13 202 13
Fragments arranged by size:
1227 926 812 599 344 319 270 202
167 141 31 13 13 6
NaeI GCC'GGC
Cuts at: 0 2824 5070
Size: 2824 2246
NcoI C'CATG_G
Cuts at: 0 3701 3914 4180 4866 5070
Size: 3701 213 266 686 204
Fragments arranged by size:
3701 686 266 213 204
NdeI CA'TA_TG
Cuts at: 0 3440 5070
Size: 3440 1630
NgoMIV G'CCGG_C
Cuts at: 0 2822 5070
Size: 2822 2248
NheI G'CTAG_C
Cuts at: 0 2002 5070
Size: 2002 3068
NspI r_CATG'y
Cuts at: 0 2232 3186 4713 5070
Size: 2232 954 1527 357
Fragments arranged by size:
2232 1527 954 357
PasI CC'CwG_GG
Cuts at: 0 18 3156 5070
Size: 18 3138 1914
Fragments arranged by size:
3138 1914 18
PciI A'CATG_T
Cuts at: 0 2228 4709 5070
Size: 2228 2481 361
Fragments arranged by size:
2481 2228 361
PflMI CCAn_nnn'nTGG
Cuts at: 0 152 2675 4541 4872 5070
Size: 152 2523 1866 331 198
Fragments arranged by size:
2523 1866 331 198 152
PfoI T'CCnGG_A
Cuts at: 0 915 4170 4271 5070
Size: 915 3255 101 799
Fragments arranged by size:
3255 915 799 101
PmlI CAC'GTG
Cuts at: 0 4105 5070
Size: 4105 965
PpuMI rG'GwC_Cy
Cuts at: 0 901 2196 3864 5046 5070
Size: 901 1295 1668 1182 24
Fragments arranged by size:
1668 1295 1182 901 24
PspOMI G'GGCC_C
Cuts at: 0 4977 5070
Size: 4977 93
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1188 1220 5070
Size: 1188 32 3850
Fragments arranged by size:
3850 1188 32
PstI C_TGCA'G
Cuts at: 0 2740 3473 3587 3690 3752 4196 5070
Size: 2740 733 114 103 62 444 874
Fragments arranged by size:
2740 874 733 444 114 103 62
PvuII CAG'CTG
Cuts at: 0 319 2358 2869 3468 3474 3615 4541 4811
Size: 319 2039 511 599 6 141 926 270
Cuts at: 4811 5070
Size: 259
Fragments arranged by size:
2039 926 599 511 319 270 259 141
6
SacII CC_GC'GG
Cuts at: 0 4843 5070
Size: 4843 227
SanDI GG'GwC_CC
Cuts at: 0 2196 5070
Size: 2196 2874
SapI GCTCTTCn'nnn_
Cuts at: 0 2562 3071 5070
Size: 2562 509 1999
Fragments arranged by size:
2562 1999 509
ScaI AGT'ACT
Cuts at: 0 1641 4404 4960 5070
Size: 1641 2763 556 110
Fragments arranged by size:
2763 1641 556 110
SfcI C'TryA_G
Cuts at: 0 5 1178 2736 3469 3583 3686 3748 4192
Size: 5 1173 1558 733 114 103 62 444
Cuts at: 4192 5070
Size: 878
Fragments arranged by size:
1558 1173 878 733 444 114 103 62
5
SgrAI Cr'CCGG_yG
Cuts at: 0 2553 5070
Size: 2553 2517
SmaI CCC'GGG
Cuts at: 0 1601 5070
Size: 1601 3469
SmlI C'TyrA_G
Cuts at: 0 701 3329 4294 4643 5070
Size: 701 2628 965 349 427
Fragments arranged by size:
2628 965 701 427 349
SphI G_CATG'C
Cuts at: 0 3186 5070
Size: 3186 1884
SspI AAT'ATT
Cuts at: 0 4200 5070
Size: 4200 870
StuI AGG'CCT
Cuts at: 0 671 1432 2809 2878 5070
Size: 671 761 1377 69 2192
Fragments arranged by size:
2192 1377 761 671 69
StyI C'CwwG_G
Cuts at: 0 108 1284 1303 1396 1815 3701 3914 4180
Size: 108 1176 19 93 419 1886 213 266
Cuts at: 4180 4673 4688 4782 4866 5070
Size: 493 15 94 84 204
Fragments arranged by size:
1886 1176 493 419 266 213 204 108
94 93 84 19 15
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 594 1511 1719 2526 2983 3148 3737 3789
Size: 594 917 208 807 457 165 589 52
Cuts at: 3789 4425 4815 4903 5070
Size: 636 390 88 167
Fragments arranged by size:
917 807 636 594 589 457 390 208
167 165 88 52
TatI w'GTAC_w
Cuts at: 0 330 1498 1639 4402 4958 5070
Size: 330 1168 141 2763 556 112
Fragments arranged by size:
2763 1168 556 330 141 112
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 800 832 1132 1164 3771 3803 3895 3927
Size: 800 32 300 32 2607 32 92 32
Cuts at: 3927 5070
Size: 1143
Fragments arranged by size:
2607 1143 800 300 92 32 32 32
32
Tth111I GACn'n_nGTC
Cuts at: 0 4332 5070
Size: 4332 738
XbaI T'CTAG_A
Cuts at: 0 3202 5070
Size: 3202 1868
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 180 1672 1958 3153 5070
Size: 180 1492 286 1195 1917
Fragments arranged by size:
1917 1492 1195 286 180
XmaI C'CCGG_G
Cuts at: 0 1599 5070
Size: 1599 3471
XmnI GAAnn'nnTTC
Cuts at: 0 4771 5070
Size: 4771 299
ZraI GAC'GTC
Cuts at: 0 2789 4224 5070
Size: 2789 1435 846
Fragments arranged by size:
2789 1435 846
Enzymes that do cut:
AarI AatII Acc65I AclI AcuI AfeI AflIII AgeI
AlfI AloI AlwNI ApaI ApaLI ApoI AseI AvaI
AvrII BaeI BamHI BanI BanII BbsI BbvCI BcgI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrDI BsrFI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EarI Eco57MI EcoNI
EcoO109I EcoRI FalI HaeII Hin4I HincII HpaI KpnI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NspI PasI PciI PflMI PfoI PmlI PpuMI
PspOMI PsrI PstI PvuII SacII SanDI SapI ScaI
SfcI SgrAI SmaI SmlI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
ZraI
Enzymes that do not cut:
AccI AflII AhdI AleI AscI AsiSI BbeI BciVI
BfrBI BsaBI BsiEI BsiWI BsrBI BsrGI BstZ17I BtgZI
EagI EciI EcoICRI EcoRV FseI FspI FspAI HindIII
KasI MfeI MluI NarI NotI NruI NsiI PacI
PmeI PpiI PshAI PsiI PspXI PvuI RsrII SacI
SalI SbfI SexAI SfiI SfoI SgfI SnaBI SpeI
SrfI SwaI XhoI