Restriction map for
pF1KA1584
(Linear) (Six-base) MAPSORT of: KIAA1584.seq Check: 9912 from: 1 to: 2898
KIAA1584.orf
With 182 enzymes: SgfI *
October 24, 2006 15:08 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1045 2898
Size: 1045 1853
AccI GT'mk_AC
Cuts at: 0 2870 2898
Size: 2870 28
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 287 524 566 581 915 1505 1570 2749
Size: 287 237 42 15 334 590 65 1179
Cuts at: 2749 2771 2898
Size: 22 127
Fragments arranged by size:
1179 590 334 287 237 127 65 42
22 15
AflII C'TTAA_G
Cuts at: 0 2091 2898
Size: 2091 807
AflIII A'CryG_T
Cuts at: 0 1161 2490 2898
Size: 1161 1329 408
Fragments arranged by size:
1329 1161 408
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 657 689 2898
Size: 657 32 2209
Fragments arranged by size:
2209 657 32
AlwNI CAG_nnn'CTG
Cuts at: 0 265 2898
Size: 265 2633
ApaLI G'TGCA_C
Cuts at: 0 188 2898
Size: 188 2710
ApoI r'AATT_y
Cuts at: 0 279 561 790 944 1114 1806 2265 2325
Size: 279 282 229 154 170 692 459 60
Cuts at: 2325 2898
Size: 573
Fragments arranged by size:
692 573 459 282 279 229 170 154
60
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 2728 2761 2898
Size: 2728 33 137
Fragments arranged by size:
2728 137 33
BamHI G'GATC_C
Cuts at: 0 717 2898
Size: 717 2181
BbsI GAAGACnn'nnnn_
Cuts at: 0 913 2898
Size: 913 1985
BbvCI CC'TCA_GC
Cuts at: 0 567 2898
Size: 567 2331
BciVI GTATCCnnnnn_n'
Cuts at: 0 405 2158 2898
Size: 405 1753 740
Fragments arranged by size:
1753 740 405
BclI T'GATC_A
Cuts at: 0 845 2550 2640 2814 2898
Size: 845 1705 90 174 84
Fragments arranged by size:
1705 845 174 90 84
BfrBI ATG'CAT
Cuts at: 0 1350 1838 2898
Size: 1350 488 1060
Fragments arranged by size:
1350 1060 488
BglII A'GATC_T
Cuts at: 0 298 2898
Size: 298 2600
Bme1580I G_kGCm'C
Cuts at: 0 192 2898
Size: 192 2706
BmtI G_CTAG'C
Cuts at: 0 625 2898
Size: 625 2273
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 575 2556 2855 2898
Size: 575 1981 299 43
Fragments arranged by size:
1981 575 299 43
Bpu10I CC'TnA_GC
Cuts at: 0 567 2898
Size: 567 2331
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1018 2761 2898
Size: 1018 1743 137
Fragments arranged by size:
1743 1018 137
BsaAI yAC'GTr
Cuts at: 0 1886 2898
Size: 1886 1012
BsaBI GATnn'nnATC
Cuts at: 0 1242 2619 2898
Size: 1242 1377 279
Fragments arranged by size:
1377 1242 279
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 46 2898
Size: 46 2852
BseYI C'CCAG_C
Cuts at: 0 157 538 1019 2898
Size: 157 381 481 1879
Fragments arranged by size:
1879 481 381 157
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2477 2898
Size: 2477 421
BsiHKAI G_wGCw'C
Cuts at: 0 192 2165 2898
Size: 192 1973 733
Fragments arranged by size:
1973 733 192
BsiWI C'GTAC_G
Cuts at: 0 1886 2898
Size: 1886 1012
BsmI GAATG_Cn'
Cuts at: 0 214 1826 2001 2898
Size: 214 1612 175 897
Fragments arranged by size:
1612 897 214 175
Bsp1286I G_dGCh'C
Cuts at: 0 192 2165 2898
Size: 192 1973 733
Fragments arranged by size:
1973 733 192
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 448 1045 2898
Size: 448 597 1853
Fragments arranged by size:
1853 597 448
BsrGI T'GTAC_A
Cuts at: 0 1390 2898
Size: 1390 1508
BssSI C'ACGA_G
Cuts at: 0 362 2898
Size: 362 2536
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2416 2898
Size: 2416 482
BstYI r'GATC_y
Cuts at: 0 298 717 2898
Size: 298 419 2181
Fragments arranged by size:
2181 419 298
BtgI C'CryG_G
Cuts at: 0 43 2898
Size: 43 2855
BtsI GCAGTG_nn'
Cuts at: 0 1017 1044 1087 2898
Size: 1017 27 43 1811
Fragments arranged by size:
1811 1017 43 27
DraI TTT'AAA
Cuts at: 0 924 2898
Size: 924 1974
EarI CTCTTCn'nnn_
Cuts at: 0 23 2898
Size: 23 2875
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 287 524 566 575 581 915 1505 1570
Size: 287 237 42 9 6 334 590 65
Cuts at: 1570 2556 2749 2771 2855 2898
Size: 986 193 22 84 43
Fragments arranged by size:
986 590 334 287 237 193 84 65
43 42 22 9 6
EcoRV GAT'ATC
Cuts at: 0 110 2898
Size: 110 2788
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 283 315 2547 2554 2579 2586 2898
Size: 283 32 2232 7 25 7 312
Fragments arranged by size:
2232 312 283 32 25 7 7
HincII GTy'rAC
Cuts at: 0 153 232 2098 2898
Size: 153 79 1866 800
Fragments arranged by size:
1866 800 153 79
HpaI GTT'AAC
Cuts at: 0 153 2898
Size: 153 2745
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 271 1506 1694 2898
Size: 271 1235 188 1204
Fragments arranged by size:
1235 1204 271 188
MspA1I CmG'CkG
Cuts at: 0 571 2898
Size: 571 2327
NcoI C'CATG_G
Cuts at: 0 43 2898
Size: 43 2855
NdeI CA'TA_TG
Cuts at: 0 1178 1212 1946 2898
Size: 1178 34 734 952
Fragments arranged by size:
1178 952 734 34
NheI G'CTAG_C
Cuts at: 0 621 2898
Size: 621 2277
NsiI A_TGCA'T
Cuts at: 0 1352 1840 2898
Size: 1352 488 1058
Fragments arranged by size:
1352 1058 488
NspI r_CATG'y
Cuts at: 0 1165 1421 2898
Size: 1165 256 1477
Fragments arranged by size:
1477 1165 256
PciI A'CATG_T
Cuts at: 0 1161 2898
Size: 1161 1737
PfoI T'CCnGG_A
Cuts at: 0 893 1494 2832 2898
Size: 893 601 1338 66
Fragments arranged by size:
1338 893 601 66
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 456 488 2898
Size: 456 32 2410
Fragments arranged by size:
2410 456 32
PsiI TTA'TAA
Cuts at: 0 1465 2898
Size: 1465 1433
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1491 1523 2898
Size: 1491 32 1375
Fragments arranged by size:
1491 1375 32
PstI C_TGCA'G
Cuts at: 0 1409 2898
Size: 1409 1489
PvuII CAG'CTG
Cuts at: 0 571 2898
Size: 571 2327
SexAI A'CCwGG_T
Cuts at: 0 339 1197 2898
Size: 339 858 1701
Fragments arranged by size:
1701 858 339
SfcI C'TryA_G
Cuts at: 0 1405 1896 1923 2898
Size: 1405 491 27 975
Fragments arranged by size:
1405 975 491 27
SmlI C'TyrA_G
Cuts at: 0 997 2091 2740 2898
Size: 997 1094 649 158
Fragments arranged by size:
1094 997 649 158
SnaBI TAC'GTA
Cuts at: 0 1886 2898
Size: 1886 1012
SspI AAT'ATT
Cuts at: 0 138 2451 2601 2898
Size: 138 2313 150 297
Fragments arranged by size:
2313 297 150 138
StyI C'CwwG_G
Cuts at: 0 43 432 1600 2898
Size: 43 389 1168 1298
Fragments arranged by size:
1298 1168 389 43
TatI w'GTAC_w
Cuts at: 0 799 1096 1390 2898
Size: 799 297 294 1508
Fragments arranged by size:
1508 799 297 294
XbaI T'CTAG_A
Cuts at: 0 295 2898
Size: 295 2603
XmnI GAAnn'nnTTC
Cuts at: 0 1163 2898
Size: 1163 1735
Enzymes that do cut:
AarI AccI AcuI AflII AflIII AloI AlwNI ApaLI
ApoI BaeI BamHI BbsI BbvCI BciVI BclI BfrBI
BglII Bme1580I BmtI BpmI Bpu10I BpuEI BsaAI BsaBI
BseRI BseYI BsgI BsiHKAI BsiWI BsmI Bsp1286I BspMI
BsrGI BssSI BstXI BstYI BtgI BtsI DraI EarI
Eco57MI EcoRV Hin4I HincII HpaI MmeI MspA1I NcoI
NdeI NheI NsiI NspI PciI PfoI PpiI PsiI
PsrI PstI PvuII SexAI SfcI SmlI SnaBI SspI
StyI TatI XbaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AgeI AhdI AleI AlfI
ApaI AscI AseI AsiSI AvaI AvrII BanI BanII
BbeI BcgI BglI BlpI BmgBI BmrI BplI BsaI
BsaHI BsaWI BsaXI BsiEI BsmBI BspEI BspHI BsrBI
BsrDI BsrFI BssHII BstAPI BstBI BstEII BstZ17I Bsu36I
BtgZI ClaI CspCI DraIII DrdI EaeI EagI EciI
EcoICRI EcoNI EcoO109I EcoRI FalI FseI FspI FspAI
HaeII HindIII KasI KpnI MfeI MluI MscI MslI
NaeI NarI NgoMIV NotI NruI PacI PasI PflMI
PmeI PmlI PpuMI PshAI PspOMI PspXI PvuI RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SfiI
SfoI SgfI SgrAI SmaI SpeI SphI SrfI StuI
SwaI TaqII TstI Tth111I XcmI XhoI XmaI ZraI