Restriction map for pF1KSDA1585
 (Linear) (Six-base) MAPSORT of: KIAA1585.seq  Check: 9265  from: 1  to: 1731



KIAA1585.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:17  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     190    1731 
   Size:        190    1541
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1621    1731 
   Size:       1621     110
 
ApoI r'AATT_y

Cuts at:      0    1136    1731 
   Size:       1136     595
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    1602    1635    1731 
   Size:       1602      33      96

  Fragments arranged by size: 

                1602      96      33
 
BamHI G'GATC_C

Cuts at:      0    1243    1731 
   Size:       1243     488
 
BbvCI CC'TCA_GC

Cuts at:      0     544    1731 
   Size:        544    1187
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     903    1731 
   Size:        903     828
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     918    1105    1648    1731 
   Size:        918     187     543      83

  Fragments arranged by size: 

                 918     543     187      83
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     806    1549    1731 
   Size:        806     743     182

  Fragments arranged by size: 

                 806     743     182
 
Bpu10I CC'TnA_GC

Cuts at:      0     544    1731 
   Size:        544    1187
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     494    1731 
   Size:        494    1237
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      62    1731 
   Size:         62    1669
 
BsaBI GATnn'nnATC

Cuts at:      0    1029    1731 
   Size:       1029     702
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     436    1731 
   Size:        436    1295
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     947    1731 
   Size:        947     784
 
BsiHKAI G_wGCw'C

Cuts at:      0      53    1731 
   Size:         53    1678
 
BsmI GAATG_Cn'

Cuts at:      0      18    1731 
   Size:         18    1713
 
Bsp1286I G_dGCh'C

Cuts at:      0      53    1731 
   Size:         53    1678
 
BspHI T'CATG_A

Cuts at:      0    1233    1731 
   Size:       1233     498
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     190    1731 
   Size:        190    1541
 
BsrDI GCAATG_nn'

Cuts at:      0     548    1598    1731 
   Size:        548    1050     133

  Fragments arranged by size: 

                1050     548     133
 
BsrGI T'GTAC_A

Cuts at:      0     982    1355    1731 
   Size:        982     373     376

  Fragments arranged by size: 

                 982     376     373
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1466    1731 
   Size:       1466     265
 
BstYI r'GATC_y

Cuts at:      0     156     687    1243    1731 
   Size:        156     531     556     488

  Fragments arranged by size: 

                 556     531     488     156
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     732     767    1731 
   Size:        732      35     964

  Fragments arranged by size: 

                 964     732      35
 
EaeI y'GGCC_r

Cuts at:      0    1508    1731 
   Size:       1508     223
 
EarI CTCTTCn'nnn_

Cuts at:      0    1148    1731 
   Size:       1148     583
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     806    1549    1731 
   Size:        806     743     182

  Fragments arranged by size: 

                 806     743     182
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1649    1731 
   Size:       1649      82
 
EcoRV GAT'ATC

Cuts at:      0     756    1731 
   Size:        756     975
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     676     708    1302    1334    1731 
   Size:        676      32     594      32     397

  Fragments arranged by size: 

                 676     594     397      32      32
 
HincII GTy'rAC

Cuts at:      0     369     672    1731 
   Size:        369     303    1059

  Fragments arranged by size: 

                1059     369     303
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     138    1731 
   Size:        138    1593
 
MscI TGG'CCA

Cuts at:      0    1510    1731 
   Size:       1510     221
 
MslI CAynn'nnrTG

Cuts at:      0     295    1238    1588    1603    1731 
   Size:        295     943     350      15     128

  Fragments arranged by size: 

                 943     350     295     128      15
 
NspI r_CATG'y

Cuts at:      0    1427    1731 
   Size:       1427     304
 
PpuMI rG'GwC_Cy

Cuts at:      0    1649    1731 
   Size:       1649      82
 
PsiI TTA'TAA

Cuts at:      0     590    1731 
   Size:        590    1141
 
PstI C_TGCA'G

Cuts at:      0     763     914    1731 
   Size:        763     151     817

  Fragments arranged by size: 

                 817     763     151
 
SanDI GG'GwC_CC

Cuts at:      0    1649    1731 
   Size:       1649      82
 
ScaI AGT'ACT

Cuts at:      0     818    1731 
   Size:        818     913
 
SfcI C'TryA_G

Cuts at:      0     240     759     910    1151    1709    1731 
   Size:        240     519     151     241     558      22

  Fragments arranged by size: 

                 558     519     241     240     151      22
 
SmlI C'TyrA_G

Cuts at:      0     509    1731 
   Size:        509    1222
 
SspI AAT'ATT

Cuts at:      0     624    1731 
   Size:        624    1107
 
StuI AGG'CCT

Cuts at:      0    1555    1731 
   Size:       1555     176
 
StyI C'CwwG_G

Cuts at:      0      74    1556    1731 
   Size:         74    1482     175

  Fragments arranged by size: 

                1482     175      74
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     183    1731 
   Size:        183    1548
 
TatI w'GTAC_w

Cuts at:      0     816     982    1200    1355    1678    1731 
   Size:        816     166     218     155     323      53

  Fragments arranged by size: 

                 816     323     218     166     155      53
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     489     521    1731 
   Size:        489      32    1210

  Fragments arranged by size: 

                1210     489      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1575    1731 
   Size:       1575     156
 
XmnI GAAnn'nnTTC

Cuts at:      0    1310    1731 
   Size:       1310     421
 

 Enzymes that do cut:

     AarI     AlfI     ApoI     BaeI    BamHI    BbvCI    BciVI     BmrI
     BpmI   Bpu10I    BpuEI     BsaI    BsaBI    BseRI     BsgI  BsiHKAI
     BsmI Bsp1286I    BspHI    BspMI    BsrDI    BsrGI   BstAPI    BstYI
    CspCI     EaeI     EarI  Eco57MI EcoO109I    EcoRV    Hin4I   HincII
     MmeI     MscI     MslI     NspI    PpuMI     PsiI     PstI    SanDI
     ScaI     SfcI     SmlI     SspI     StuI     StyI    TaqII     TatI
     TstI     XcmI     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AloI    AlwNI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BanI    BanII     BbeI     BbsI
     BcgI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI
     BmtI     BplI    BsaAI    BsaHI    BsaWI    BsaXI    BseYI    BsiEI
    BsiWI    BsmBI    BspEI    BsrBI    BsrFI   BssHII    BssSI    BstBI
   BstEII    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI
     DraI   DraIII     DrdI     EagI     EciI  EcoICRI    EcoNI    EcoRI
     FalI     FseI     FspI    FspAI    HaeII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI   MspA1I     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI     PciI
    PflMI     PfoI     PmeI     PmlI     PpiI    PshAI   PspOMI    PspXI
     PsrI     PvuI    PvuII    RsrII     SacI    SacII     SalI     SapI
     SbfI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI
     SpeI     SphI     SrfI     SwaI  Tth111I     XbaI     XhoI     XmaI
     ZraI
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