Restriction map for
pF1KA1597
(Linear) (Six-base) MAPSORT of: KIAA1597.seq Check: 2982 from: 1 to: 2685
KIAA1597.orf
With 182 enzymes: SgfI *
October 24, 2006 15:08 ..
Acc65I G'GTAC_C
Cuts at: 0 2138 2685
Size: 2138 547
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 49 112 1583 1828 2167 2465 2567 2579
Size: 49 63 1471 245 339 298 102 12
Cuts at: 2579 2685
Size: 106
Fragments arranged by size:
1471 339 298 245 106 102 63 49
12
AleI CACnn'nnGTG
Cuts at: 0 2422 2685
Size: 2422 263
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 35 2685
Size: 35 2650
AlwNI CAG_nnn'CTG
Cuts at: 0 90 2233 2685
Size: 90 2143 452
Fragments arranged by size:
2143 452 90
ApaLI G'TGCA_C
Cuts at: 0 2263 2685
Size: 2263 422
ApoI r'AATT_y
Cuts at: 0 881 1018 1260 1616 2319 2685
Size: 881 137 242 356 703 366
Fragments arranged by size:
881 703 366 356 242 137
AseI AT'TA_AT
Cuts at: 0 1205 2685
Size: 1205 1480
AvaI C'yCGr_G
Cuts at: 0 339 2685
Size: 339 2346
AvrII C'CTAG_G
Cuts at: 0 2070 2685
Size: 2070 615
BamHI G'GATC_C
Cuts at: 0 767 2685
Size: 767 1918
BanI G'GyrC_C
Cuts at: 0 1380 2138 2685
Size: 1380 758 547
Fragments arranged by size:
1380 758 547
BanII G_rGCy'C
Cuts at: 0 759 2685
Size: 759 1926
BbsI GAAGACnn'nnnn_
Cuts at: 0 914 1499 2161 2380 2512 2685
Size: 914 585 662 219 132 173
Fragments arranged by size:
914 662 585 219 173 132
BciVI GTATCCnnnnn_n'
Cuts at: 0 2040 2685
Size: 2040 645
BglII A'GATC_T
Cuts at: 0 2448 2685
Size: 2448 237
BlpI GC'TnA_GC
Cuts at: 0 209 2685
Size: 209 2476
Bme1580I G_kGCm'C
Cuts at: 0 2267 2685
Size: 2267 418
BmrI ACTGGGnnnn_n'
Cuts at: 0 1424 1827 2115 2685
Size: 1424 403 288 570
Fragments arranged by size:
1424 570 403 288
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 773 787 2685
Size: 773 14 1898
Fragments arranged by size:
1898 773 14
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 269 401 768 952 2194 2276 2685
Size: 269 132 367 184 1242 82 409
Fragments arranged by size:
1242 409 367 269 184 132 82
Bpu10I CC'TnA_GC
Cuts at: 0 1097 1367 2302 2685
Size: 1097 270 935 383
Fragments arranged by size:
1097 935 383 270
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 757 1128 2685
Size: 757 371 1557
Fragments arranged by size:
1557 757 371
BsaI GGTCTCn'nnnn_
Cuts at: 0 1394 2685
Size: 1394 1291
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2192 2222 2685
Size: 2192 30 463
Fragments arranged by size:
2192 463 30
BseYI C'CCAG_C
Cuts at: 0 248 2685
Size: 248 2437
BsiHKAI G_wGCw'C
Cuts at: 0 2267 2685
Size: 2267 418
BsmI GAATG_Cn'
Cuts at: 0 897 1215 1609 2287 2663 2685
Size: 897 318 394 678 376 22
Fragments arranged by size:
897 678 394 376 318 22
Bsp1286I G_dGCh'C
Cuts at: 0 759 2267 2685
Size: 759 1508 418
Fragments arranged by size:
1508 759 418
BstAPI GCAn_nnn'nTGC
Cuts at: 0 615 2167 2685
Size: 615 1552 518
Fragments arranged by size:
1552 615 518
BstYI r'GATC_y
Cuts at: 0 767 2448 2685
Size: 767 1681 237
Fragments arranged by size:
1681 767 237
BtgI C'CryG_G
Cuts at: 0 153 999 2685
Size: 153 846 1686
Fragments arranged by size:
1686 846 153
BtsI GCAGTG_nn'
Cuts at: 0 968 1728 2296 2644 2685
Size: 968 760 568 348 41
Fragments arranged by size:
968 760 568 348 41
DraI TTT'AAA
Cuts at: 0 695 2685
Size: 695 1990
EaeI y'GGCC_r
Cuts at: 0 105 2685
Size: 105 2580
EarI CTCTTCn'nnn_
Cuts at: 0 25 38 184 302 311 418 921 1073
Size: 25 13 146 118 9 107 503 152
Cuts at: 1073 2685
Size: 1612
Fragments arranged by size:
1612 503 152 146 118 107 25 13
9
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 49 112 269 401 768 952 1583 1828
Size: 49 63 157 132 367 184 631 245
Cuts at: 1828 2167 2194 2276 2465 2567 2579 2685
Size: 339 27 82 189 102 12 106
Fragments arranged by size:
631 367 339 245 189 184 157 132
106 102 82 63 49 27 12
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2074 2685
Size: 2074 611
EcoO109I rG'GnC_Cy
Cuts at: 0 194 635 2685
Size: 194 441 2050
Fragments arranged by size:
2050 441 194
EcoRI G'AATT_C
Cuts at: 0 1260 2685
Size: 1260 1425
EcoRV GAT'ATC
Cuts at: 0 165 2685
Size: 165 2520
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1971 2685
Size: 1971 714
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 417 449 529 561 697 729 1279 1311
Size: 417 32 80 32 136 32 550 32
Cuts at: 1311 1333 1365 1486 1518 2685
Size: 22 32 121 32 1167
Fragments arranged by size:
1167 550 417 136 121 80 32 32
32 32 32 32 22
HincII GTy'rAC
Cuts at: 0 264 566 2685
Size: 264 302 2119
Fragments arranged by size:
2119 302 264
HindIII A'AGCT_T
Cuts at: 0 2242 2685
Size: 2242 443
KpnI G_GTAC'C
Cuts at: 0 2142 2685
Size: 2142 543
MfeI C'AATT_G
Cuts at: 0 1787 2128 2685
Size: 1787 341 557
Fragments arranged by size:
1787 557 341
MscI TGG'CCA
Cuts at: 0 107 2685
Size: 107 2578
MslI CAynn'nnrTG
Cuts at: 0 2272 2422 2685
Size: 2272 150 263
Fragments arranged by size:
2272 263 150
MspA1I CmG'CkG
Cuts at: 0 15 90 208 1855 2685
Size: 15 75 118 1647 830
Fragments arranged by size:
1647 830 118 75 15
NcoI C'CATG_G
Cuts at: 0 153 999 2685
Size: 153 846 1686
Fragments arranged by size:
1686 846 153
NdeI CA'TA_TG
Cuts at: 0 1152 1884 2685
Size: 1152 732 801
Fragments arranged by size:
1152 801 732
NspI r_CATG'y
Cuts at: 0 949 1822 2685
Size: 949 873 863
Fragments arranged by size:
949 873 863
PflMI CCAn_nnn'nTGG
Cuts at: 0 1207 2233 2421 2511 2630 2685
Size: 1207 1026 188 90 119 55
Fragments arranged by size:
1207 1026 188 119 90 55
PpuMI rG'GwC_Cy
Cuts at: 0 635 2685
Size: 635 2050
PshAI GACnn'nnGTC
Cuts at: 0 1248 2685
Size: 1248 1437
PsiI TTA'TAA
Cuts at: 0 821 2685
Size: 821 1864
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 2419 2451 2685
Size: 2419 32 234
Fragments arranged by size:
2419 234 32
PstI C_TGCA'G
Cuts at: 0 963 2685
Size: 963 1722
PvuII CAG'CTG
Cuts at: 0 90 208 2685
Size: 90 118 2477
Fragments arranged by size:
2477 118 90
SanDI GG'GwC_CC
Cuts at: 0 635 2685
Size: 635 2050
SapI GCTCTTCn'nnn_
Cuts at: 0 302 2685
Size: 302 2383
SfcI C'TryA_G
Cuts at: 0 959 2384 2685
Size: 959 1425 301
Fragments arranged by size:
1425 959 301
SmlI C'TyrA_G
Cuts at: 0 772 1143 2685
Size: 772 371 1542
Fragments arranged by size:
1542 772 371
SspI AAT'ATT
Cuts at: 0 1776 2685
Size: 1776 909
StuI AGG'CCT
Cuts at: 0 623 1894 2442 2685
Size: 623 1271 548 243
Fragments arranged by size:
1271 623 548 243
StyI C'CwwG_G
Cuts at: 0 125 153 999 2070 2685
Size: 125 28 846 1071 615
Fragments arranged by size:
1071 846 615 125 28
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 240 2262 2685
Size: 240 2022 423
Fragments arranged by size:
2022 423 240
XbaI T'CTAG_A
Cuts at: 0 1396 1488 2685
Size: 1396 92 1197
Fragments arranged by size:
1396 1197 92
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2508 2685
Size: 2508 177
XmnI GAAnn'nnTTC
Cuts at: 0 812 1082 1119 2685
Size: 812 270 37 1566
Fragments arranged by size:
1566 812 270 37
Enzymes that do cut:
Acc65I AcuI AleI AlfI AlwNI ApaLI ApoI AseI
AvaI AvrII BamHI BanI BanII BbsI BciVI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaXI BseYI BsiHKAI BsmI Bsp1286I BstAPI BstYI BtgI
BtsI DraI EaeI EarI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI Hin4I HincII HindIII KpnI MfeI MscI
MslI MspA1I NcoI NdeI NspI PflMI PpuMI PshAI
PsiI PsrI PstI PvuII SanDI SapI SfcI SmlI
SspI StuI StyI TaqII XbaI XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AflIII AgeI
AhdI AloI ApaI AscI AsiSI BaeI BbeI BbvCI
BcgI BclI BfrBI BglI BmgBI BmtI BsaAI BsaBI
BsaHI BsaWI BseRI BsgI BsiEI BsiWI BsmBI BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstBI BstEII BstXI BstZ17I Bsu36I BtgZI ClaI CspCI
DraIII DrdI EagI EciI EcoICRI FseI FspI FspAI
HaeII HpaI KasI MluI MmeI NaeI NarI NgoMIV
NheI NotI NruI NsiI PacI PasI PciI PfoI
PmeI PmlI PpiI PspOMI PspXI PvuI RsrII SacI
SacII SalI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SwaI TatI
TstI Tth111I XhoI XmaI ZraI