Restriction map for
pF1KSDA1609
(Linear) (Six-base) MAPSORT of: KIAA1609.seq Check: 751 from: 1 to: 1368
KIAA1609.orf
With 182 enzymes: SgfI *
October 24, 2006 15:17 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 477 1368
Size: 477 891
AccI GT'mk_AC
Cuts at: 0 218 1368
Size: 218 1150
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 160 1368
Size: 160 1208
AflIII A'CryG_T
Cuts at: 0 254 1034 1368
Size: 254 780 334
Fragments arranged by size:
780 334 254
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 21 53 583 615 1368
Size: 21 32 530 32 753
Fragments arranged by size:
753 530 32 32 21
AlwNI CAG_nnn'CTG
Cuts at: 0 438 492 558 1305 1368
Size: 438 54 66 747 63
Fragments arranged by size:
747 438 66 63 54
ApaI G_GGCC'C
Cuts at: 0 473 551 872 1368
Size: 473 78 321 496
Fragments arranged by size:
496 473 321 78
ApaLI G'TGCA_C
Cuts at: 0 412 1158 1368
Size: 412 746 210
Fragments arranged by size:
746 412 210
AvaI C'yCGr_G
Cuts at: 0 479 776 886 1368
Size: 479 297 110 482
Fragments arranged by size:
482 479 297 110
BanI G'GyrC_C
Cuts at: 0 788 797 1368
Size: 788 9 571
Fragments arranged by size:
788 571 9
BanII G_rGCy'C
Cuts at: 0 473 551 821 872 1346 1368
Size: 473 78 270 51 474 22
Fragments arranged by size:
474 473 270 78 51 22
BbeI G_GCGC'C
Cuts at: 0 801 1368
Size: 801 567
BclI T'GATC_A
Cuts at: 0 72 1368
Size: 72 1296
BglI GCCn_nnn'nGGC
Cuts at: 0 555 1368
Size: 555 813
BlpI GC'TnA_GC
Cuts at: 0 421 500 1368
Size: 421 79 868
Fragments arranged by size:
868 421 79
Bme1580I G_kGCm'C
Cuts at: 0 416 473 551 872 1162 1368
Size: 416 57 78 321 290 206
Fragments arranged by size:
416 321 290 206 78 57
BmgBI CAC'GTC
Cuts at: 0 159 1368
Size: 159 1209
BmrI ACTGGGnnnn_n'
Cuts at: 0 239 461 546 603 1084 1368
Size: 239 222 85 57 481 284
Fragments arranged by size:
481 284 239 222 85 57
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1330 1368
Size: 1330 38
Bpu10I CC'TnA_GC
Cuts at: 0 695 1190 1368
Size: 695 495 178
Fragments arranged by size:
695 495 178
BsaI GGTCTCn'nnnn_
Cuts at: 0 1229 1368
Size: 1229 139
BsaAI yAC'GTr
Cuts at: 0 417 1157 1166 1368
Size: 417 740 9 202
Fragments arranged by size:
740 417 202 9
BsaHI Gr'CG_yC
Cuts at: 0 798 1368
Size: 798 570
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 21 51 820 850 1368
Size: 21 30 769 30 518
Fragments arranged by size:
769 518 30 30 21
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 325 1261 1368
Size: 325 936 107
Fragments arranged by size:
936 325 107
BseYI C'CCAG_C
Cuts at: 0 767 839 991 1368
Size: 767 72 152 377
Fragments arranged by size:
767 377 152 72
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 505 1368
Size: 505 863
BsiHKAI G_wGCw'C
Cuts at: 0 416 667 821 1162 1368
Size: 416 251 154 341 206
Fragments arranged by size:
416 341 251 206 154
BsmI GAATG_Cn'
Cuts at: 0 1323 1368
Size: 1323 45
BsmBI CGTCTCn'nnnn_
Cuts at: 0 674 1368
Size: 674 694
Bsp1286I G_dGCh'C
Cuts at: 0 416 473 551 667 821 872 1162 1346
Size: 416 57 78 116 154 51 290 184
Cuts at: 1346 1368
Size: 22
Fragments arranged by size:
416 290 184 154 116 78 57 51
22
BspHI T'CATG_A
Cuts at: 0 323 1368
Size: 323 1045
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 477 1368
Size: 477 891
BsrGI T'GTAC_A
Cuts at: 0 755 1004 1368
Size: 755 249 364
Fragments arranged by size:
755 364 249
BstEII G'GTnAC_C
Cuts at: 0 186 1368
Size: 186 1182
BstXI CCAn_nnnn'nTGG
Cuts at: 0 847 1368
Size: 847 521
BstYI r'GATC_y
Cuts at: 0 393 1368
Size: 393 975
Bsu36I CC'TnA_GG
Cuts at: 0 53 1292 1368
Size: 53 1239 76
Fragments arranged by size:
1239 76 53
BtgI C'CryG_G
Cuts at: 0 822 1047 1368
Size: 822 225 321
Fragments arranged by size:
822 321 225
EaeI y'GGCC_r
Cuts at: 0 617 849 1256 1368
Size: 617 232 407 112
Fragments arranged by size:
617 407 232 112
EarI CTCTTCn'nnn_
Cuts at: 0 176 310 1368
Size: 176 134 1058
Fragments arranged by size:
1058 176 134
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 42 1368
Size: 42 1326
EcoICRI GAG'CTC
Cuts at: 0 819 1368
Size: 819 549
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 160 1330 1368
Size: 160 1170 38
Fragments arranged by size:
1170 160 38
EcoO109I rG'GnC_Cy
Cuts at: 0 241 355 469 547 548 606 868 869
Size: 241 114 114 78 1 58 262 1
Cuts at: 869 1302 1368
Size: 433 66
Fragments arranged by size:
433 262 241 114 114 78 66 58
1 1
HaeII r_GCGC'y
Cuts at: 0 801 1368
Size: 801 567
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 76 108 241 273 569 601 730 762
Size: 76 32 133 32 296 32 129 32
Cuts at: 762 1368
Size: 606
Fragments arranged by size:
606 296 133 129 76 32 32 32
32
HincII GTy'rAC
Cuts at: 0 219 1368
Size: 219 1149
KasI G'GCGC_C
Cuts at: 0 797 1368
Size: 797 571
MfeI C'AATT_G
Cuts at: 0 76 1368
Size: 76 1292
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1212 1368
Size: 1212 156
MscI TGG'CCA
Cuts at: 0 619 851 1258 1368
Size: 619 232 407 110
Fragments arranged by size:
619 407 232 110
MslI CAynn'nnrTG
Cuts at: 0 328 1000 1368
Size: 328 672 368
Fragments arranged by size:
672 368 328
MspA1I CmG'CkG
Cuts at: 0 504 771 786 794 1182 1368
Size: 504 267 15 8 388 186
Fragments arranged by size:
504 388 267 186 15 8
NarI GG'CG_CC
Cuts at: 0 798 1368
Size: 798 570
NcoI C'CATG_G
Cuts at: 0 822 1047 1368
Size: 822 225 321
Fragments arranged by size:
822 321 225
NspI r_CATG'y
Cuts at: 0 210 907 1038 1368
Size: 210 697 131 330
Fragments arranged by size:
697 330 210 131
PasI CC'CwG_GG
Cuts at: 0 466 1368
Size: 466 902
PciI A'CATG_T
Cuts at: 0 1034 1368
Size: 1034 334
PflMI CCAn_nnn'nTGG
Cuts at: 0 558 1368
Size: 558 810
PfoI T'CCnGG_A
Cuts at: 0 734 1277 1346 1355 1368
Size: 734 543 69 9 13
Fragments arranged by size:
734 543 69 13 9
PmlI CAC'GTG
Cuts at: 0 417 1157 1368
Size: 417 740 211
Fragments arranged by size:
740 417 211
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 21 53 1368
Size: 21 32 1315
Fragments arranged by size:
1315 32 21
PpuMI rG'GwC_Cy
Cuts at: 0 241 355 606 1368
Size: 241 114 251 762
Fragments arranged by size:
762 251 241 114
PspOMI G'GGCC_C
Cuts at: 0 469 547 868 1368
Size: 469 78 321 500
Fragments arranged by size:
500 469 321 78
PspXI vC'TCGA_Gb
Cuts at: 0 886 1368
Size: 886 482
PvuII CAG'CTG
Cuts at: 0 504 771 1182 1368
Size: 504 267 411 186
Fragments arranged by size:
504 411 267 186
SacI G_AGCT'C
Cuts at: 0 821 1368
Size: 821 547
SalI G'TCGA_C
Cuts at: 0 217 1368
Size: 217 1151
SanDI GG'GwC_CC
Cuts at: 0 241 606 1368
Size: 241 365 762
Fragments arranged by size:
762 365 241
SapI GCTCTTCn'nnn_
Cuts at: 0 176 1368
Size: 176 1192
SfcI C'TryA_G
Cuts at: 0 148 1368
Size: 148 1220
SfoI GGC'GCC
Cuts at: 0 799 1368
Size: 799 569
SmaI CCC'GGG
Cuts at: 0 481 1368
Size: 481 887
SmlI C'TyrA_G
Cuts at: 0 886 1368
Size: 886 482
SphI G_CATG'C
Cuts at: 0 210 1368
Size: 210 1158
StyI C'CwwG_G
Cuts at: 0 822 1047 1259 1368
Size: 822 225 212 109
Fragments arranged by size:
822 225 212 109
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 585 590 1101 1368
Size: 585 5 511 267
Fragments arranged by size:
585 511 267 5
TatI w'GTAC_w
Cuts at: 0 755 1004 1037 1368
Size: 755 249 33 331
Fragments arranged by size:
755 331 249 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 339 371 820 852 1368
Size: 339 32 449 32 516
Fragments arranged by size:
516 449 339 32 32
XhoI C'TCGA_G
Cuts at: 0 886 1368
Size: 886 482
XmaI C'CCGG_G
Cuts at: 0 479 1368
Size: 479 889
XmnI GAAnn'nnTTC
Cuts at: 0 170 1368
Size: 170 1198
Enzymes that do cut:
AarI AccI AcuI AflIII AloI AlwNI ApaI ApaLI
AvaI BanI BanII BbeI BclI BglI BlpI Bme1580I
BmgBI BmrI BpmI Bpu10I BsaI BsaAI BsaHI BsaXI
BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspHI
BspMI BsrGI BstEII BstXI BstYI Bsu36I BtgI EaeI
EarI EciI EcoICRI Eco57MI EcoO109I HaeII Hin4I HincII
KasI MfeI MmeI MscI MslI MspA1I NarI NcoI
NspI PasI PciI PflMI PfoI PmlI PpiI PpuMI
PspOMI PspXI PvuII SacI SalI SanDI SapI SfcI
SfoI SmaI SmlI SphI StyI TaqII TatI TstI
XhoI XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AhdI AleI
AlfI ApoI AscI AseI AsiSI AvrII BaeI BamHI
BbsI BbvCI BcgI BciVI BfrBI BglII BmtI BplI
BpuEI BsaBI BsaWI BsiEI BsiWI BspEI BsrBI BsrDI
BsrFI BssHII BssSI BstAPI BstBI BstZ17I BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EagI EcoNI EcoRI
EcoRV FalI FseI FspI FspAI HindIII HpaI KpnI
MluI NaeI NdeI NgoMIV NheI NotI NruI NsiI
PacI PmeI PshAI PsiI PsrI PstI PvuI RsrII
SacII SbfI ScaI SexAI SfiI SgfI SgrAI SnaBI
SpeI SrfI SspI StuI SwaI Tth111I XbaI XcmI
ZraI