Restriction map for
pF1KA1611
(Linear) (Six-base) MAPSORT of: KIAA1611.seq Check: 3940 from: 1 to: 2454
KIAA1611.orf
With 182 enzymes: SgfI *
March 3, 2008 10:55 ..
AccI GT'mk_AC
Cuts at: 0 628 2454
Size: 628 1826
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 948 1368 1536 2124 2376 2454
Size: 948 420 168 588 252 78
Fragments arranged by size:
948 588 420 252 168 78
AleI CACnn'nnGTG
Cuts at: 0 346 2454
Size: 346 2108
ApoI r'AATT_y
Cuts at: 0 46 665 804 830 998 1140 1166 1476
Size: 46 619 139 26 168 142 26 310
Cuts at: 1476 1502 1670 1922 2258 2454
Size: 26 168 252 336 196
Fragments arranged by size:
619 336 310 252 196 168 168 142
139 46 26 26 26
BanII G_rGCy'C
Cuts at: 0 197 2454
Size: 197 2257
BbsI GAAGACnn'nnnn_
Cuts at: 0 386 954 1710 2046 2382 2454
Size: 386 568 756 336 336 72
Fragments arranged by size:
756 568 386 336 336 72
BfrBI ATG'CAT
Cuts at: 0 1392 1980 2430 2454
Size: 1392 588 450 24
Fragments arranged by size:
1392 588 450 24
BmgBI CAC'GTC
Cuts at: 0 105 2454
Size: 105 2349
BmrI ACTGGGnnnn_n'
Cuts at: 0 2192 2276 2454
Size: 2192 84 178
Fragments arranged by size:
2192 178 84
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1655 2454
Size: 1655 799
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 944 1028 1112 1196 1280 1532 1616 1700
Size: 944 84 84 84 84 252 84 84
Cuts at: 1700 1784 1868 1952 2036 2372 2454
Size: 84 84 84 84 336 82
Fragments arranged by size:
944 336 252 84 84 84 84 84
84 84 84 84 84 82
BsaWI w'CCGG_w
Cuts at: 0 2098 2434 2454
Size: 2098 336 20
Fragments arranged by size:
2098 336 20
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 73 2454
Size: 73 2381
BsmI GAATG_Cn'
Cuts at: 0 1392 1459 1879 1980 2432 2454
Size: 1392 67 420 101 452 22
Fragments arranged by size:
1392 452 420 101 67 22
Bsp1286I G_dGCh'C
Cuts at: 0 197 2454
Size: 197 2257
BspHI T'CATG_A
Cuts at: 0 705 945 1869 2454
Size: 705 240 924 585
Fragments arranged by size:
924 705 585 240
BsrDI GCAATG_nn'
Cuts at: 0 1203 1455 2127 2454
Size: 1203 252 672 327
Fragments arranged by size:
1203 672 327 252
BsrGI T'GTAC_A
Cuts at: 0 286 2287 2454
Size: 286 2001 167
Fragments arranged by size:
2001 286 167
BstAPI GCAn_nnn'nTGC
Cuts at: 0 866 1202 1454 2454
Size: 866 336 252 1000
Fragments arranged by size:
1000 866 336 252
BstBI TT'CG_AA
Cuts at: 0 808 2454
Size: 808 1646
BstYI r'GATC_y
Cuts at: 0 89 2454
Size: 89 2365
BtsI GCAGTG_nn'
Cuts at: 0 867 2454
Size: 867 1587
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2109 2144 2454
Size: 2109 35 310
Fragments arranged by size:
2109 310 35
DraIII CAC_nnn'GTG
Cuts at: 0 345 2454
Size: 345 2109
EaeI y'GGCC_r
Cuts at: 0 38 2454
Size: 38 2416
EarI CTCTTCn'nnn_
Cuts at: 0 202 310 501 1135 2454
Size: 202 108 191 634 1319
Fragments arranged by size:
1319 634 202 191 108
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 944 948 1028 1112 1196 1280 1368 1532
Size: 944 4 80 84 84 84 88 164
Cuts at: 1532 1536 1616 1700 1784 1868 1952 2036 2124
Size: 4 80 84 84 84 84 84 88
Cuts at: 2124 2372 2376 2454
Size: 248 4 78
Fragments arranged by size:
944 248 164 88 88 84 84 84
84 84 84 84 84 80 80 78
4 4 4
EcoNI CCTnn'n_nnAGG
Cuts at: 0 10 2454
Size: 10 2444
EcoRI G'AATT_C
Cuts at: 0 46 804 830 998 1166 1502 1670 1922
Size: 46 758 26 168 168 336 168 252
Cuts at: 1922 2258 2454
Size: 336 196
Fragments arranged by size:
758 336 336 252 196 168 168 168
46 26
EcoRV GAT'ATC
Cuts at: 0 273 2454
Size: 273 2181
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1841 2454
Size: 1841 613
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1563 1595 2391 2423 2454
Size: 1563 32 796 32 31
Fragments arranged by size:
1563 796 32 32 31
HincII GTy'rAC
Cuts at: 0 23 2454
Size: 23 2431
HindIII A'AGCT_T
Cuts at: 0 523 549 2454
Size: 523 26 1905
Fragments arranged by size:
1905 523 26
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 92 158 332 2454
Size: 92 66 174 2122
Fragments arranged by size:
2122 174 92 66
MscI TGG'CCA
Cuts at: 0 40 2454
Size: 40 2414
MslI CAynn'nnrTG
Cuts at: 0 346 950 1343 2454
Size: 346 604 393 1111
Fragments arranged by size:
1111 604 393 346
NdeI CA'TA_TG
Cuts at: 0 2336 2454
Size: 2336 118
NsiI A_TGCA'T
Cuts at: 0 1394 1982 2432 2454
Size: 1394 588 450 22
Fragments arranged by size:
1394 588 450 22
PflMI CCAn_nnn'nTGG
Cuts at: 0 345 2454
Size: 345 2109
PsiI TTA'TAA
Cuts at: 0 770 1153 2454
Size: 770 383 1301
Fragments arranged by size:
1301 770 383
PstI C_TGCA'G
Cuts at: 0 578 2454
Size: 578 1876
SapI GCTCTTCn'nnn_
Cuts at: 0 202 310 1135 2454
Size: 202 108 825 1319
Fragments arranged by size:
1319 825 202 108
SfcI C'TryA_G
Cuts at: 0 574 2454
Size: 574 1880
SspI AAT'ATT
Cuts at: 0 165 2454
Size: 165 2289
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2179 2454
Size: 2179 275
TatI w'GTAC_w
Cuts at: 0 286 322 2287 2454
Size: 286 36 1965 167
Fragments arranged by size:
1965 286 167 36
Tth111I GACn'n_nGTC
Cuts at: 0 146 2454
Size: 146 2308
XmnI GAAnn'nnTTC
Cuts at: 0 1549 1674 2137 2454
Size: 1549 125 463 317
Fragments arranged by size:
1549 463 317 125
Enzymes that do cut:
AccI AcuI AleI ApoI BanII BbsI BfrBI BmgBI
BmrI BplI BpmI BsaWI BseRI BsmI Bsp1286I BspHI
BsrDI BsrGI BstAPI BstBI BstYI BtsI CspCI DraIII
EaeI EarI Eco57MI EcoNI EcoRI EcoRV FalI Hin4I
HincII HindIII MmeI MscI MslI NdeI NsiI PflMI
PsiI PstI SapI SfcI SspI TaqII TatI Tth111I
XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AlfI AloI AlwNI ApaI ApaLI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BanI BbeI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmtI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaXI BseYI
BsgI BsiEI BsiHKAI BsiWI BsmBI BspEI BspMI BsrBI
BsrFI BssHII BssSI BstEII BstXI BstZ17I Bsu36I BtgI
BtgZI ClaI DraI DrdI EagI EciI EcoICRI EcoO109I
FseI FspI FspAI HaeII HpaI KasI KpnI MfeI
MluI MspA1I NaeI NarI NcoI NgoMIV NheI NotI
NruI NspI PacI PasI PciI PfoI PmeI PmlI
PpiI PpuMI PshAI PspOMI PspXI PsrI PvuI PvuII
RsrII SacI SacII SalI SanDI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI StuI StyI SwaI TstI XbaI XcmI
XhoI XmaI ZraI