Restriction map for
pF1KSDA1614
(Linear) (Six-base) MAPSORT of: KIAA1614.seq Check: 9418 from: 1 to: 3570
KIAA1614.orf
With 182 enzymes: SgfI *
October 24, 2006 15:17 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2036 3570
Size: 2036 1534
AatII G_ACGT'C
Cuts at: 0 3121 3570
Size: 3121 449
AclI AA'CG_TT
Cuts at: 0 926 3570
Size: 926 2644
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1996 2111 2429 3570
Size: 1996 115 318 1141
Fragments arranged by size:
1996 1141 318 115
AfeI AGC'GCT
Cuts at: 0 823 3412 3570
Size: 823 2589 158
Fragments arranged by size:
2589 823 158
AflIII A'CryG_T
Cuts at: 0 2904 3570
Size: 2904 666
AgeI A'CCGG_T
Cuts at: 0 3233 3570
Size: 3233 337
AhdI GACnn_n'nnGTC
Cuts at: 0 1067 3570
Size: 1067 2503
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1054 1453 3570
Size: 1054 399 2117
Fragments arranged by size:
2117 1054 399
AlwNI CAG_nnn'CTG
Cuts at: 0 412 1425 2515 2561 2896 2932 3570
Size: 412 1013 1090 46 335 36 638
Fragments arranged by size:
1090 1013 638 412 335 46 36
ApaI G_GGCC'C
Cuts at: 0 56 238 614 1333 1459 2716 3135 3570
Size: 56 182 376 719 126 1257 419 435
Fragments arranged by size:
1257 719 435 419 376 182 126 56
AvaI C'yCGr_G
Cuts at: 0 1026 1226 1961 2657 3570
Size: 1026 200 735 696 913
Fragments arranged by size:
1026 913 735 696 200
AvrII C'CTAG_G
Cuts at: 0 2032 3570
Size: 2032 1538
BamHI G'GATC_C
Cuts at: 0 1778 3570
Size: 1778 1792
BanI G'GyrC_C
Cuts at: 0 1030 1575 1584 1604 1656 2418 2609 3388
Size: 1030 545 9 20 52 762 191 779
Cuts at: 3388 3458 3570
Size: 70 112
Fragments arranged by size:
1030 779 762 545 191 112 70 52
20 9
BanII G_rGCy'C
Cuts at: 0 56 238 553 614 634 943 1096 1333
Size: 56 182 315 61 20 309 153 237
Cuts at: 1333 1459 1503 1688 1732 1760 1954 2000 2675
Size: 126 44 185 44 28 194 46 675
Cuts at: 2675 2716 3135 3570
Size: 41 419 435
Fragments arranged by size:
675 435 419 315 309 237 194 185
182 153 126 61 56 46 44 44
41 28 20
BbeI G_GCGC'C
Cuts at: 0 1660 2422 3462 3570
Size: 1660 762 1040 108
Fragments arranged by size:
1660 1040 762 108
BbvCI CC'TCA_GC
Cuts at: 0 96 1988 3491 3570
Size: 96 1892 1503 79
Fragments arranged by size:
1892 1503 96 79
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1608 1642 3570
Size: 1608 34 1928
Fragments arranged by size:
1928 1608 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 2533 3570
Size: 2533 1037
BglI GCCn_nnn'nGGC
Cuts at: 0 447 1208 3570
Size: 447 761 2362
Fragments arranged by size:
2362 761 447
BglII A'GATC_T
Cuts at: 0 756 3570
Size: 756 2814
BlpI GC'TnA_GC
Cuts at: 0 1090 1426 2784 3217 3570
Size: 1090 336 1358 433 353
Fragments arranged by size:
1358 1090 433 353 336
Bme1580I G_kGCm'C
Cuts at: 0 56 238 614 1033 1134 1184 1277 1316
Size: 56 182 376 419 101 50 93 39
Cuts at: 1316 1333 1459 1813 2716 3135 3315 3427 3570
Size: 17 126 354 903 419 180 112 143
Fragments arranged by size:
903 419 419 376 354 182 180 143
126 112 101 93 56 50 39 17
BmgBI CAC'GTC
Cuts at: 0 529 3330 3570
Size: 529 2801 240
Fragments arranged by size:
2801 529 240
BmrI ACTGGGnnnn_n'
Cuts at: 0 1029 2157 3570
Size: 1029 1128 1413
Fragments arranged by size:
1413 1128 1029
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 111 244 1279 1842 3570
Size: 111 133 1035 563 1728
Fragments arranged by size:
1728 1035 563 133 111
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 214 545 846 854 862 1249 1275 1414
Size: 214 331 301 8 8 387 26 139
Cuts at: 1414 1663 1672 1938 2155 2764 2918 3570
Size: 249 9 266 217 609 154 652
Fragments arranged by size:
652 609 387 331 301 266 249 217
214 154 139 26 9 8 8
Bpu10I CC'TnA_GC
Cuts at: 0 96 119 1726 1988 2491 3491 3514 3570
Size: 96 23 1607 262 503 1000 23 56
Fragments arranged by size:
1607 1000 503 262 96 56 23 23
BsaI GGTCTCn'nnnn_
Cuts at: 0 830 3570
Size: 830 2740
BsaAI yAC'GTr
Cuts at: 0 1347 3570
Size: 1347 2223
BsaHI Gr'CG_yC
Cuts at: 0 1657 2260 2419 3118 3459 3570
Size: 1657 603 159 699 341 111
Fragments arranged by size:
1657 699 603 341 159 111
BsaWI w'CCGG_w
Cuts at: 0 2730 3233 3570
Size: 2730 503 337
Fragments arranged by size:
2730 503 337
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1727 1757 1790 1820 2833 2863 2891 2921
Size: 1727 30 33 30 1013 30 28 30
Cuts at: 2921 3570
Size: 649
Fragments arranged by size:
1727 1013 649 33 30 30 30 30
28
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 413 487 799 1614 1891 2854 3473 3570
Size: 413 74 312 815 277 963 619 97
Fragments arranged by size:
963 815 619 413 312 277 97 74
BseYI C'CCAG_C
Cuts at: 0 200 606 613 700 813 856 1221 1716
Size: 200 406 7 87 113 43 365 495
Cuts at: 1716 1901 2018 2184 2414 2496 2585 2590 3060
Size: 185 117 166 230 82 89 5 470
Cuts at: 3060 3340 3503 3570
Size: 280 163 67
Fragments arranged by size:
495 470 406 365 280 230 200 185
166 163 117 113 89 87 82 67
43 7 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 974 3136 3296 3570
Size: 974 2162 160 274
Fragments arranged by size:
2162 974 274 160
BsiHKAI G_wGCw'C
Cuts at: 0 1432 1688 1732 2000 3339 3570
Size: 1432 256 44 268 1339 231
Fragments arranged by size:
1432 1339 268 256 231 44
Bsp1286I G_dGCh'C
Cuts at: 0 56 238 553 614 634 943 1033 1096
Size: 56 182 315 61 20 309 90 63
Cuts at: 1096 1134 1184 1277 1316 1333 1432 1459 1503
Size: 38 50 93 39 17 99 27 44
Cuts at: 1503 1688 1732 1760 1813 1954 2000 2675 2716
Size: 185 44 28 53 141 46 675 41
Cuts at: 2716 3135 3315 3339 3427 3570
Size: 419 180 24 88 143
Fragments arranged by size:
675 419 315 309 185 182 180 143
141 99 93 90 88 63 61 56
53 50 46 44 44 41 39 38
28 27 24 20 17
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 446 2036 3570
Size: 446 1590 1534
Fragments arranged by size:
1590 1534 446
BsrBI CCG'CTC
Cuts at: 0 1398 3570
Size: 1398 2172
BsrFI r'CCGG_y
Cuts at: 0 1581 3233 3288 3570
Size: 1581 1652 55 282
Fragments arranged by size:
1652 1581 282 55
BssSI C'ACGA_G
Cuts at: 0 1278 2308 2692 3570
Size: 1278 1030 384 878
Fragments arranged by size:
1278 1030 878 384
BstAPI GCAn_nnn'nTGC
Cuts at: 0 918 1122 2028 2616 2794 3570
Size: 918 204 906 588 178 776
Fragments arranged by size:
918 906 776 588 204 178
BstEII G'GTnAC_C
Cuts at: 0 2500 3570
Size: 2500 1070
BstXI CCAn_nnnn'nTGG
Cuts at: 0 84 155 192 3570
Size: 84 71 37 3378
Fragments arranged by size:
3378 84 71 37
BstYI r'GATC_y
Cuts at: 0 756 1778 2893 3570
Size: 756 1022 1115 677
Fragments arranged by size:
1115 1022 756 677
Bsu36I CC'TnA_GG
Cuts at: 0 167 2600 3570
Size: 167 2433 970
Fragments arranged by size:
2433 970 167
BtgI C'CryG_G
Cuts at: 0 32 83 553 639 1445 2810 3304 3356
Size: 32 51 470 86 806 1365 494 52
Cuts at: 3356 3498 3570
Size: 142 72
Fragments arranged by size:
1365 806 494 470 142 86 72 52
51 32
BtsI GCAGTG_nn'
Cuts at: 0 870 2614 3570
Size: 870 1744 956
Fragments arranged by size:
1744 956 870
DraIII CAC_nnn'GTG
Cuts at: 0 1808 3159 3310 3570
Size: 1808 1351 151 260
Fragments arranged by size:
1808 1351 260 151
DrdI GACnn_nn'nnGTC
Cuts at: 0 717 3123 3570
Size: 717 2406 447
Fragments arranged by size:
2406 717 447
EaeI y'GGCC_r
Cuts at: 0 23 287 1050 1209 3290 3570
Size: 23 264 763 159 2081 280
Fragments arranged by size:
2081 763 280 264 159 23
EarI CTCTTCn'nnn_
Cuts at: 0 3031 3491 3570
Size: 3031 460 79
Fragments arranged by size:
3031 460 79
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1356 1929 2008 3570
Size: 1356 573 79 1562
Fragments arranged by size:
1562 1356 573 79
EcoICRI GAG'CTC
Cuts at: 0 1686 1730 1998 3570
Size: 1686 44 268 1572
Fragments arranged by size:
1686 1572 268 44
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 214 545 846 854 862 1249 1275 1414
Size: 214 331 301 8 8 387 26 139
Cuts at: 1414 1663 1672 1938 1996 2111 2155 2429 2764
Size: 249 9 266 58 115 44 274 335
Cuts at: 2764 2918 3570
Size: 154 652
Fragments arranged by size:
652 387 335 331 301 274 266 249
214 154 139 115 58 44 26 9
8 8
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2177 3370 3570
Size: 2177 1193 200
Fragments arranged by size:
2177 1193 200
EcoO109I rG'GnC_Cy
Cuts at: 0 52 92 234 235 496 610 667 719
Size: 52 40 142 1 261 114 57 52
Cuts at: 719 1148 1230 1329 1330 1455 1456 1673 1886
Size: 429 82 99 1 125 1 217 213
Cuts at: 1886 2007 2158 2558 2712 2713 2764 2941 3131
Size: 121 151 400 154 1 51 177 190
Cuts at: 3131 3132 3530 3570
Size: 1 398 40
Fragments arranged by size:
429 400 398 261 217 213 190 177
154 151 142 125 121 114 99 82
57 52 52 51 40 40 1 1
1 1 1
FseI GG_CCGG'CC
Cuts at: 0 3292 3570
Size: 3292 278
HaeII r_GCGC'y
Cuts at: 0 825 857 1413 1419 1660 2422 3414 3462
Size: 825 32 556 6 241 762 992 48
Cuts at: 3462 3570
Size: 108
Fragments arranged by size:
992 825 762 556 241 108 48 32
6
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 316 348 754 786 1009 1041 1053 1085
Size: 316 32 406 32 223 32 12 32
Cuts at: 1085 1348 1380 2833 2865 3570
Size: 263 32 1453 32 705
Fragments arranged by size:
1453 705 406 316 263 223 32 32
32 32 32 32 12
KasI G'GCGC_C
Cuts at: 0 1656 2418 3458 3570
Size: 1656 762 1040 112
Fragments arranged by size:
1656 1040 762 112
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 3160 3570
Size: 3160 410
MscI TGG'CCA
Cuts at: 0 289 1211 3570
Size: 289 922 2359
Fragments arranged by size:
2359 922 289
MspA1I CmG'CkG
Cuts at: 0 34 100 129 453 603 988 1295 1307
Size: 34 66 29 324 150 385 307 12
Cuts at: 1307 1447 1622 3280 3399 3405 3570
Size: 140 175 1658 119 6 165
Fragments arranged by size:
1658 385 324 307 175 165 150 140
119 66 34 29 12 6
NaeI GCC'GGC
Cuts at: 0 1583 3290 3570
Size: 1583 1707 280
Fragments arranged by size:
1707 1583 280
NarI GG'CG_CC
Cuts at: 0 1657 2419 3459 3570
Size: 1657 762 1040 111
Fragments arranged by size:
1657 1040 762 111
NcoI C'CATG_G
Cuts at: 0 639 3498 3570
Size: 639 2859 72
Fragments arranged by size:
2859 639 72
NgoMIV G'CCGG_C
Cuts at: 0 1581 3288 3570
Size: 1581 1707 282
Fragments arranged by size:
1707 1581 282
NspI r_CATG'y
Cuts at: 0 2908 3570
Size: 2908 662
PasI CC'CwG_GG
Cuts at: 0 209 1194 1647 2154 2769 3009 3036 3418
Size: 209 985 453 507 615 240 27 382
Cuts at: 3418 3570
Size: 152
Fragments arranged by size:
985 615 507 453 382 240 209 152
27
PciI A'CATG_T
Cuts at: 0 2904 3570
Size: 2904 666
PflMI CCAn_nnn'nTGG
Cuts at: 0 215 3570
Size: 215 3355
PfoI T'CCnGG_A
Cuts at: 0 651 1511 1679 1781 2215 3570
Size: 651 860 168 102 434 1355
Fragments arranged by size:
1355 860 651 434 168 102
PmlI CAC'GTG
Cuts at: 0 1347 3570
Size: 1347 2223
PpuMI rG'GwC_Cy
Cuts at: 0 92 667 719 1148 1230 1673 1886 2158
Size: 92 575 52 429 82 443 213 272
Cuts at: 2158 2558 2764 2941 3570
Size: 400 206 177 629
Fragments arranged by size:
629 575 443 429 400 272 213 206
177 92 82 52
PshAI GACnn'nnGTC
Cuts at: 0 596 3570
Size: 596 2974
PspOMI G'GGCC_C
Cuts at: 0 52 234 610 1329 1455 2712 3131 3570
Size: 52 182 376 719 126 1257 419 439
Fragments arranged by size:
1257 719 439 419 376 182 126 52
PspXI vC'TCGA_Gb
Cuts at: 0 1961 3570
Size: 1961 1609
PstI C_TGCA'G
Cuts at: 0 914 1127 1255 1406 1755 1864 2669 2722
Size: 914 213 128 151 349 109 805 53
Cuts at: 2722 2879 3404 3410 3570
Size: 157 525 6 160
Fragments arranged by size:
914 805 525 349 213 160 157 151
128 109 53 6
PvuII CAG'CTG
Cuts at: 0 100 129 453 988 1622 3280 3399 3405
Size: 100 29 324 535 634 1658 119 6
Cuts at: 3405 3570
Size: 165
Fragments arranged by size:
1658 634 535 324 165 119 100 29
6
SacI G_AGCT'C
Cuts at: 0 1688 1732 2000 3570
Size: 1688 44 268 1570
Fragments arranged by size:
1688 1570 268 44
SacII CC_GC'GG
Cuts at: 0 35 1448 3570
Size: 35 1413 2122
Fragments arranged by size:
2122 1413 35
SanDI GG'GwC_CC
Cuts at: 0 1230 2158 3570
Size: 1230 928 1412
Fragments arranged by size:
1412 1230 928
SapI GCTCTTCn'nnn_
Cuts at: 0 3031 3570
Size: 3031 539
ScaI AGT'ACT
Cuts at: 0 535 3570
Size: 535 3035
SexAI A'CCwGG_T
Cuts at: 0 3248 3570
Size: 3248 322
SfcI C'TryA_G
Cuts at: 0 910 1123 1251 1402 1751 1860 2665 2718
Size: 910 213 128 151 349 109 805 53
Cuts at: 2718 2875 3078 3400 3406 3570
Size: 157 203 322 6 164
Fragments arranged by size:
910 805 349 322 213 203 164 157
151 128 109 53 6
SfoI GGC'GCC
Cuts at: 0 1658 2420 3460 3570
Size: 1658 762 1040 110
Fragments arranged by size:
1658 1040 762 110
SmaI CCC'GGG
Cuts at: 0 1228 3570
Size: 1228 2342
SmlI C'TyrA_G
Cuts at: 0 1961 3570
Size: 1961 1609
StuI AGG'CCT
Cuts at: 0 1612 1959 3570
Size: 1612 347 1611
Fragments arranged by size:
1612 1611 347
StyI C'CwwG_G
Cuts at: 0 48 254 639 706 1920 2002 2032 3498
Size: 48 206 385 67 1214 82 30 1466
Cuts at: 3498 3570
Size: 72
Fragments arranged by size:
1466 1214 385 206 82 72 67 48
30
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 805 1708 1882 2488 2627 3322 3570
Size: 805 903 174 606 139 695 248
Fragments arranged by size:
903 805 695 606 248 174 139
TatI w'GTAC_w
Cuts at: 0 222 533 3570
Size: 222 311 3037
Fragments arranged by size:
3037 311 222
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1727 1759 1790 1822 1857 1889 1960 1992
Size: 1727 32 31 32 35 32 71 32
Cuts at: 1992 2891 2923 3570
Size: 899 32 647
Fragments arranged by size:
1727 899 647 71 35 32 32 32
32 32 31
Tth111I GACn'n_nGTC
Cuts at: 0 2886 3570
Size: 2886 684
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 569 1696 2170 2378 2776 3246 3277 3570
Size: 569 1127 474 208 398 470 31 293
Fragments arranged by size:
1127 569 474 470 398 293 208 31
XhoI C'TCGA_G
Cuts at: 0 1961 3570
Size: 1961 1609
XmaI C'CCGG_G
Cuts at: 0 1226 3570
Size: 1226 2344
ZraI GAC'GTC
Cuts at: 0 3119 3570
Size: 3119 451
Enzymes that do cut:
AarI AatII AclI AcuI AfeI AflIII AgeI AhdI
AlfI AlwNI ApaI AvaI AvrII BamHI BanI BanII
BbeI BbvCI BcgI BciVI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BsaI BsaAI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspMI
BsrBI BsrFI BssSI BstAPI BstEII BstXI BstYI Bsu36I
BtgI BtsI DraIII DrdI EaeI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I FseI HaeII Hin4I KasI MmeI
MscI MspA1I NaeI NarI NcoI NgoMIV NspI PasI
PciI PflMI PfoI PmlI PpuMI PshAI PspOMI PspXI
PstI PvuII SacI SacII SanDI SapI ScaI SexAI
SfcI SfoI SmaI SmlI StuI StyI TaqII TatI
TstI Tth111I XcmI XhoI XmaI ZraI
Enzymes that do not cut:
AccI Acc65I AflII AleI AloI ApaLI ApoI AscI
AseI AsiSI BaeI BbsI BclI BfrBI BmtI BpuEI
BsaBI BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrDI
BsrGI BssHII BstBI BstZ17I BtgZI ClaI CspCI DraI
EagI EcoRI EcoRV FalI FspI FspAI HincII HindIII
HpaI KpnI MfeI MluI MslI NdeI NheI NotI
NruI NsiI PacI PmeI PpiI PsiI PsrI PvuI
RsrII SalI SbfI SfiI SgfI SgrAI SnaBI SpeI
SphI SrfI SspI SwaI XbaI XmnI