Restriction map for
pF1KSDA1617
(Linear) (Six-base) MAPSORT of: KIAA1617.seq Check: 3031 from: 1 to: 2682
KIAA1617.orf
With 182 enzymes: SgfI *
October 24, 2006 15:17 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 119 565 752 1819 2626 2682
Size: 119 446 187 1067 807 56
Fragments arranged by size:
1067 807 446 187 119 56
AflIII A'CryG_T
Cuts at: 0 192 266 2682
Size: 192 74 2416
Fragments arranged by size:
2416 192 74
AhdI GACnn_n'nnGTC
Cuts at: 0 2241 2682
Size: 2241 441
AleI CACnn'nnGTG
Cuts at: 0 2482 2682
Size: 2482 200
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1696 1890 2682
Size: 1696 194 792
Fragments arranged by size:
1696 792 194
AlwNI CAG_nnn'CTG
Cuts at: 0 309 1504 2682
Size: 309 1195 1178
Fragments arranged by size:
1195 1178 309
ApaLI G'TGCA_C
Cuts at: 0 399 2682
Size: 399 2283
ApoI r'AATT_y
Cuts at: 0 466 841 1779 1869 1911 2682
Size: 466 375 938 90 42 771
Fragments arranged by size:
938 771 466 375 90 42
AvaI C'yCGr_G
Cuts at: 0 2142 2401 2682
Size: 2142 259 281
Fragments arranged by size:
2142 281 259
BanI G'GyrC_C
Cuts at: 0 1082 2253 2349 2682
Size: 1082 1171 96 333
Fragments arranged by size:
1171 1082 333 96
BanII G_rGCy'C
Cuts at: 0 941 2143 2219 2306 2682
Size: 941 1202 76 87 376
Fragments arranged by size:
1202 941 376 87 76
BbeI G_GCGC'C
Cuts at: 0 2353 2682
Size: 2353 329
BbsI GAAGACnn'nnnn_
Cuts at: 0 2108 2682
Size: 2108 574
BbvCI CC'TCA_GC
Cuts at: 0 1586 2364 2682
Size: 1586 778 318
Fragments arranged by size:
1586 778 318
BglI GCCn_nnn'nGGC
Cuts at: 0 2521 2682
Size: 2521 161
BglII A'GATC_T
Cuts at: 0 2009 2682
Size: 2009 673
BlpI GC'TnA_GC
Cuts at: 0 69 2682
Size: 69 2613
Bme1580I G_kGCm'C
Cuts at: 0 403 2258 2389 2517 2682
Size: 403 1855 131 128 165
Fragments arranged by size:
1855 403 165 131 128
BmgBI CAC'GTC
Cuts at: 0 2484 2682
Size: 2484 198
BmrI ACTGGGnnnn_n'
Cuts at: 0 144 282 1152 1381 2664 2682
Size: 144 138 870 229 1283 18
Fragments arranged by size:
1283 870 229 144 138 18
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1120 1974 2226 2274 2682
Size: 1120 854 252 48 408
Fragments arranged by size:
1120 854 408 252 48
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2470 2682
Size: 2470 212
Bpu10I CC'TnA_GC
Cuts at: 0 1586 2364 2682
Size: 1586 778 318
Fragments arranged by size:
1586 778 318
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 93 937 2682
Size: 93 844 1745
Fragments arranged by size:
1745 844 93
BsaI GGTCTCn'nnnn_
Cuts at: 0 80 2197 2682
Size: 80 2117 485
Fragments arranged by size:
2117 485 80
BsaAI yAC'GTr
Cuts at: 0 269 296 478 2682
Size: 269 27 182 2204
Fragments arranged by size:
2204 269 182 27
BsaHI Gr'CG_yC
Cuts at: 0 2174 2350 2682
Size: 2174 176 332
Fragments arranged by size:
2174 332 176
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1100 2176 2215 2352 2443 2446 2449 2682
Size: 1100 1076 39 137 91 3 3 233
Fragments arranged by size:
1100 1076 233 137 91 39 3 3
BseYI C'CCAG_C
Cuts at: 0 2610 2682
Size: 2610 72
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1409 2119 2608 2682
Size: 1409 710 489 74
Fragments arranged by size:
1409 710 489 74
BsiHKAI G_wGCw'C
Cuts at: 0 10 403 2219 2682
Size: 10 393 1816 463
Fragments arranged by size:
1816 463 393 10
BsiWI C'GTAC_G
Cuts at: 0 1845 2682
Size: 1845 837
BsmI GAATG_Cn'
Cuts at: 0 206 2682
Size: 206 2476
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1786 2682
Size: 1786 896
Bsp1286I G_dGCh'C
Cuts at: 0 10 403 941 2143 2219 2258 2306 2389
Size: 10 393 538 1202 76 39 48 83
Cuts at: 2389 2517 2682
Size: 128 165
Fragments arranged by size:
1202 538 393 165 128 83 76 48
39 10
BspHI T'CATG_A
Cuts at: 0 1521 2682
Size: 1521 1161
BsrBI CCG'CTC
Cuts at: 0 2294 2682
Size: 2294 388
BsrFI r'CCGG_y
Cuts at: 0 2206 2312 2352 2682
Size: 2206 106 40 330
Fragments arranged by size:
2206 330 106 40
BstAPI GCAn_nnn'nTGC
Cuts at: 0 169 309 2682
Size: 169 140 2373
Fragments arranged by size:
2373 169 140
BstEII G'GTnAC_C
Cuts at: 0 2475 2682
Size: 2475 207
BstXI CCAn_nnnn'nTGG
Cuts at: 0 516 731 1079 1394 2682
Size: 516 215 348 315 1288
Fragments arranged by size:
1288 516 348 315 215
BstYI r'GATC_y
Cuts at: 0 1767 2009 2682
Size: 1767 242 673
Fragments arranged by size:
1767 673 242
BtgI C'CryG_G
Cuts at: 0 389 1373 2123 2135 2177 2343 2682
Size: 389 984 750 12 42 166 339
Fragments arranged by size:
984 750 389 339 166 42 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 357 2682
Size: 357 2325
DraIII CAC_nnn'GTG
Cuts at: 0 390 2682
Size: 390 2292
EaeI y'GGCC_r
Cuts at: 0 278 1128 2314 2522 2553 2682
Size: 278 850 1186 208 31 129
Fragments arranged by size:
1186 850 278 208 129 31
EarI CTCTTCn'nnn_
Cuts at: 0 146 854 1447 1597 1784 2153 2682
Size: 146 708 593 150 187 369 529
Fragments arranged by size:
708 593 529 369 187 150 146
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2085 2263 2682
Size: 2085 178 419
Fragments arranged by size:
2085 419 178
EcoICRI GAG'CTC
Cuts at: 0 2217 2682
Size: 2217 465
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 119 565 752 1819 2470 2626 2682
Size: 119 446 187 1067 651 156 56
Fragments arranged by size:
1067 651 446 187 156 119 56
EcoNI CCTnn'n_nnAGG
Cuts at: 0 530 1115 2682
Size: 530 585 1567
Fragments arranged by size:
1567 585 530
EcoO109I rG'GnC_Cy
Cuts at: 0 523 541 1618 2614 2682
Size: 523 18 1077 996 68
Fragments arranged by size:
1077 996 523 68 18
EcoRV GAT'ATC
Cuts at: 0 1934 2682
Size: 1934 748
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1717 2682
Size: 1717 965
HaeII r_GCGC'y
Cuts at: 0 2353 2682
Size: 2353 329
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 25 57 664 696 1354 1386 2227 2259
Size: 25 32 607 32 658 32 841 32
Cuts at: 2259 2682
Size: 423
Fragments arranged by size:
841 658 607 423 32 32 32 32
25
HincII GTy'rAC
Cuts at: 0 1566 2682
Size: 1566 1116
HindIII A'AGCT_T
Cuts at: 0 111 913 2682
Size: 111 802 1769
Fragments arranged by size:
1769 802 111
KasI G'GCGC_C
Cuts at: 0 2349 2682
Size: 2349 333
MfeI C'AATT_G
Cuts at: 0 1486 2682
Size: 1486 1196
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 615 2444 2605 2682
Size: 615 1829 161 77
Fragments arranged by size:
1829 615 161 77
MscI TGG'CCA
Cuts at: 0 280 1130 2524 2682
Size: 280 850 1394 158
Fragments arranged by size:
1394 850 280 158
MslI CAynn'nnrTG
Cuts at: 0 2095 2482 2682
Size: 2095 387 200
Fragments arranged by size:
2095 387 200
MspA1I CmG'CkG
Cuts at: 0 306 1758 2048 2137 2345 2682
Size: 306 1452 290 89 208 337
Fragments arranged by size:
1452 337 306 290 208 89
NaeI GCC'GGC
Cuts at: 0 2314 2354 2682
Size: 2314 40 328
Fragments arranged by size:
2314 328 40
NarI GG'CG_CC
Cuts at: 0 2350 2682
Size: 2350 332
NcoI C'CATG_G
Cuts at: 0 1373 2177 2682
Size: 1373 804 505
Fragments arranged by size:
1373 804 505
NgoMIV G'CCGG_C
Cuts at: 0 2312 2352 2682
Size: 2312 40 330
Fragments arranged by size:
2312 330 40
PfoI T'CCnGG_A
Cuts at: 0 515 1263 1784 2219 2682
Size: 515 748 521 435 463
Fragments arranged by size:
748 521 515 463 435
PmlI CAC'GTG
Cuts at: 0 296 2682
Size: 296 2386
PpuMI rG'GwC_Cy
Cuts at: 0 523 2682
Size: 523 2159
PstI C_TGCA'G
Cuts at: 0 1337 2682
Size: 1337 1345
PvuII CAG'CTG
Cuts at: 0 306 1758 2682
Size: 306 1452 924
Fragments arranged by size:
1452 924 306
SacI G_AGCT'C
Cuts at: 0 2219 2682
Size: 2219 463
SacII CC_GC'GG
Cuts at: 0 2138 2346 2682
Size: 2138 208 336
Fragments arranged by size:
2138 336 208
SapI GCTCTTCn'nnn_
Cuts at: 0 1597 2682
Size: 1597 1085
ScaI AGT'ACT
Cuts at: 0 607 2682
Size: 607 2075
SfcI C'TryA_G
Cuts at: 0 545 1333 2682
Size: 545 788 1349
Fragments arranged by size:
1349 788 545
SfoI GGC'GCC
Cuts at: 0 2351 2682
Size: 2351 331
SmlI C'TyrA_G
Cuts at: 0 108 916 2682
Size: 108 808 1766
Fragments arranged by size:
1766 808 108
SspI AAT'ATT
Cuts at: 0 145 2682
Size: 145 2537
StyI C'CwwG_G
Cuts at: 0 50 1373 2177 2519 2682
Size: 50 1323 804 342 163
Fragments arranged by size:
1323 804 342 163 50
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 264 2682
Size: 264 2418
TatI w'GTAC_w
Cuts at: 0 449 605 808 1074 2682
Size: 449 156 203 266 1608
Fragments arranged by size:
1608 449 266 203 156
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 603 635 2682
Size: 603 32 2047
Fragments arranged by size:
2047 603 32
Tth111I GACn'n_nGTC
Cuts at: 0 367 1861 2682
Size: 367 1494 821
Fragments arranged by size:
1494 821 367
Enzymes that do cut:
AcuI AflIII AhdI AleI AlfI AlwNI ApaLI ApoI
AvaI BanI BanII BbeI BbsI BbvCI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaHI BseRI BseYI BsgI BsiHKAI BsiWI
BsmI BsmBI Bsp1286I BspHI BsrBI BsrFI BstAPI BstEII
BstXI BstYI BtgI BtgZI DraIII EaeI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRV FalI HaeII Hin4I
HincII HindIII KasI MfeI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV PfoI PmlI PpuMI PstI
PvuII SacI SacII SapI ScaI SfcI SfoI SmlI
SspI StyI TaqII TatI TstI Tth111I
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AloI ApaI AscI AseI AsiSI AvrII BaeI BamHI
BcgI BciVI BclI BfrBI BmtI BsaBI BsaWI BsaXI
BsiEI BspEI BspMI BsrDI BsrGI BssHII BssSI BstBI
BstZ17I Bsu36I BtsI ClaI CspCI DraI DrdI EagI
EcoRI FseI FspI FspAI HpaI KpnI MluI NdeI
NheI NotI NruI NsiI NspI PacI PasI PciI
PflMI PmeI PpiI PshAI PsiI PspOMI PspXI PsrI
PvuI RsrII SalI SanDI SbfI SexAI SfiI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI StuI SwaI
XbaI XcmI XhoI XmaI XmnI ZraI