Restriction map for
pF1KA1619
(Linear) (Six-base) MAPSORT of: KIAA1619.seq Check: 2556 from: 1 to: 2529
KIAA1619.orf
With 182 enzymes: SgfI *
March 3, 2008 10:55 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1741 2529
Size: 1741 788
AflIII A'CryG_T
Cuts at: 0 1240 2529
Size: 1240 1289
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2460 2529
Size: 2460 69
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 640 672 2529
Size: 640 32 1857
Fragments arranged by size:
1857 640 32
AlwNI CAG_nnn'CTG
Cuts at: 0 46 2529
Size: 46 2483
ApaI G_GGCC'C
Cuts at: 0 2444 2529
Size: 2444 85
ApoI r'AATT_y
Cuts at: 0 559 2529
Size: 559 1970
AvaI C'yCGr_G
Cuts at: 0 215 1559 1787 2529
Size: 215 1344 228 742
Fragments arranged by size:
1344 742 228 215
BamHI G'GATC_C
Cuts at: 0 1361 2529
Size: 1361 1168
BanI G'GyrC_C
Cuts at: 0 220 1104 1588 2022 2135 2529
Size: 220 884 484 434 113 394
Fragments arranged by size:
884 484 434 394 220 113
BanII G_rGCy'C
Cuts at: 0 128 216 264 751 1115 1387 1488 2301
Size: 128 88 48 487 364 272 101 813
Cuts at: 2301 2444 2524 2529
Size: 143 80 5
Fragments arranged by size:
813 487 364 272 143 128 101 88
80 48 5
BbeI G_GCGC'C
Cuts at: 0 2139 2529
Size: 2139 390
BbsI GAAGACnn'nnnn_
Cuts at: 0 991 1729 2529
Size: 991 738 800
Fragments arranged by size:
991 800 738
BbvCI CC'TCA_GC
Cuts at: 0 28 192 440 999 2529
Size: 28 164 248 559 1530
Fragments arranged by size:
1530 559 248 164 28
BciVI GTATCCnnnnn_n'
Cuts at: 0 1692 2529
Size: 1692 837
BfrBI ATG'CAT
Cuts at: 0 394 630 2529
Size: 394 236 1899
Fragments arranged by size:
1899 394 236
BglI GCCn_nnn'nGGC
Cuts at: 0 796 2170 2264 2373 2529
Size: 796 1374 94 109 156
Fragments arranged by size:
1374 796 156 109 94
BlpI GC'TnA_GC
Cuts at: 0 970 2339 2529
Size: 970 1369 190
Fragments arranged by size:
1369 970 190
Bme1580I G_kGCm'C
Cuts at: 0 225 1238 1348 2016 2444 2529
Size: 225 1013 110 668 428 85
Fragments arranged by size:
1013 668 428 225 110 85
BmrI ACTGGGnnnn_n'
Cuts at: 0 288 1425 2164 2529
Size: 288 1137 739 365
Fragments arranged by size:
1137 739 365 288
BmtI G_CTAG'C
Cuts at: 0 2053 2529
Size: 2053 476
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 207 765 2529
Size: 207 558 1764
Fragments arranged by size:
1764 558 207
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 202 1012 1500 2240 2260 2281 2529
Size: 202 810 488 740 20 21 248
Fragments arranged by size:
810 740 488 248 202 21 20
Bpu10I CC'TnA_GC
Cuts at: 0 28 192 440 999 1109 1822 1886 2529
Size: 28 164 248 559 110 713 64 643
Fragments arranged by size:
713 643 559 248 164 110 64 28
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 921 1246 2529
Size: 921 325 1283
Fragments arranged by size:
1283 921 325
BsaI GGTCTCn'nnnn_
Cuts at: 0 66 809 1556 2529
Size: 66 743 747 973
Fragments arranged by size:
973 747 743 66
BsaAI yAC'GTr
Cuts at: 0 1243 2529
Size: 1243 1286
BsaHI Gr'CG_yC
Cuts at: 0 2136 2529
Size: 2136 393
BsaWI w'CCGG_w
Cuts at: 0 563 2529
Size: 563 1966
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 586 616 640 670 1417 1447 2529
Size: 586 30 24 30 747 30 1082
Fragments arranged by size:
1082 747 586 30 30 30 24
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 13 16 199 484 631 757 2082 2089
Size: 13 3 183 285 147 126 1325 7
Cuts at: 2089 2529
Size: 440
Fragments arranged by size:
1325 440 285 183 147 126 13 7
3
BseYI C'CCAG_C
Cuts at: 0 498 1082 1577 1983 2251 2301 2368 2529
Size: 498 584 495 406 268 50 67 161
Fragments arranged by size:
584 498 495 406 268 161 67 50
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 446 710 2529
Size: 446 264 1819
Fragments arranged by size:
1819 446 264
BsiHKAI G_wGCw'C
Cuts at: 0 128 976 1739 1892 2524 2529
Size: 128 848 763 153 632 5
Fragments arranged by size:
848 763 632 153 128 5
BsmI GAATG_Cn'
Cuts at: 0 569 2529
Size: 569 1960
BsmBI CGTCTCn'nnnn_
Cuts at: 0 877 2529
Size: 877 1652
Bsp1286I G_dGCh'C
Cuts at: 0 128 216 225 264 751 976 1115 1238
Size: 128 88 9 39 487 225 139 123
Cuts at: 1238 1348 1387 1488 1739 1892 2016 2301 2444
Size: 110 39 101 251 153 124 285 143
Cuts at: 2444 2524 2529
Size: 80 5
Fragments arranged by size:
487 285 251 225 153 143 139 128
124 123 110 101 88 80 39 39
9 5
BspEI T'CCGG_A
Cuts at: 0 563 2529
Size: 563 1966
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1340 2529
Size: 1340 1189
BsrBI CCG'CTC
Cuts at: 0 1730 2529
Size: 1730 799
BsrDI GCAATG_nn'
Cuts at: 0 1141 2054 2529
Size: 1141 913 475
Fragments arranged by size:
1141 913 475
BsrFI r'CCGG_y
Cuts at: 0 2334 2372 2529
Size: 2334 38 157
Fragments arranged by size:
2334 157 38
BssSI C'ACGA_G
Cuts at: 0 667 800 1125 2500 2529
Size: 667 133 325 1375 29
Fragments arranged by size:
1375 667 325 133 29
BstXI CCAn_nnnn'nTGG
Cuts at: 0 632 693 2218 2355 2529
Size: 632 61 1525 137 174
Fragments arranged by size:
1525 632 174 137 61
BstYI r'GATC_y
Cuts at: 0 272 833 1028 1361 2179 2529
Size: 272 561 195 333 818 350
Fragments arranged by size:
818 561 350 333 272 195
BtgI C'CryG_G
Cuts at: 0 504 2529
Size: 504 2025
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1841 2529
Size: 1841 688
BtsI GCAGTG_nn'
Cuts at: 0 64 172 698 901 2362 2529
Size: 64 108 526 203 1461 167
Fragments arranged by size:
1461 526 203 167 108 64
DraIII CAC_nnn'GTG
Cuts at: 0 786 907 980 2529
Size: 786 121 73 1549
Fragments arranged by size:
1549 786 121 73
EaeI y'GGCC_r
Cuts at: 0 1966 2529
Size: 1966 563
EarI CTCTTCn'nnn_
Cuts at: 0 116 1400 2529
Size: 116 1284 1129
Fragments arranged by size:
1284 1129 116
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 571 2529
Size: 571 1958
EcoICRI GAG'CTC
Cuts at: 0 126 2522 2529
Size: 126 2396 7
Fragments arranged by size:
2396 126 7
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 202 1012 1500 1741 2240 2260 2281 2529
Size: 202 810 488 241 499 20 21 248
Fragments arranged by size:
810 499 488 248 241 202 21 20
EcoNI CCTnn'n_nnAGG
Cuts at: 0 195 2529
Size: 195 2334
EcoO109I rG'GnC_Cy
Cuts at: 0 58 134 1048 1581 1628 2256 2440 2441
Size: 58 76 914 533 47 628 184 1
Cuts at: 2441 2529
Size: 88
Fragments arranged by size:
914 628 533 184 88 76 58 47
1
EcoRI G'AATT_C
Cuts at: 0 559 2529
Size: 559 1970
FspI TGC'GCA
Cuts at: 0 1147 1602 2529
Size: 1147 455 927
Fragments arranged by size:
1147 927 455
HaeII r_GCGC'y
Cuts at: 0 1083 2139 2529
Size: 1083 1056 390
Fragments arranged by size:
1083 1056 390
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1165 1197 1417 1449 1659 1691 1759 1791
Size: 1165 32 220 32 210 32 68 32
Cuts at: 1791 2506 2529
Size: 715 23
Fragments arranged by size:
1165 715 220 210 68 32 32 32
32 23
KasI G'GCGC_C
Cuts at: 0 2135 2529
Size: 2135 394
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1802 2529
Size: 1802 727
MslI CAynn'nnrTG
Cuts at: 0 389 953 2529
Size: 389 564 1576
Fragments arranged by size:
1576 564 389
MspA1I CmG'CkG
Cuts at: 0 369 1351 1520 2019 2270 2529
Size: 369 982 169 499 251 259
Fragments arranged by size:
982 499 369 259 251 169
NaeI GCC'GGC
Cuts at: 0 2374 2529
Size: 2374 155
NarI GG'CG_CC
Cuts at: 0 2136 2529
Size: 2136 393
NgoMIV G'CCGG_C
Cuts at: 0 2372 2529
Size: 2372 157
NheI G'CTAG_C
Cuts at: 0 2049 2529
Size: 2049 480
NsiI A_TGCA'T
Cuts at: 0 396 632 2529
Size: 396 236 1897
Fragments arranged by size:
1897 396 236
NspI r_CATG'y
Cuts at: 0 398 2529
Size: 398 2131
PasI CC'CwG_GG
Cuts at: 0 1632 2287 2529
Size: 1632 655 242
Fragments arranged by size:
1632 655 242
PflMI CCAn_nnn'nTGG
Cuts at: 0 505 1437 2529
Size: 505 932 1092
Fragments arranged by size:
1092 932 505
PfoI T'CCnGG_A
Cuts at: 0 1190 2217 2474 2529
Size: 1190 1027 257 55
Fragments arranged by size:
1190 1027 257 55
PmlI CAC'GTG
Cuts at: 0 1243 2529
Size: 1243 1286
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 640 672 2529
Size: 640 32 1857
Fragments arranged by size:
1857 640 32
PpuMI rG'GwC_Cy
Cuts at: 0 58 134 1048 1581 1628 2529
Size: 58 76 914 533 47 901
Fragments arranged by size:
914 901 533 76 58 47
PshAI GACnn'nnGTC
Cuts at: 0 1300 2529
Size: 1300 1229
PspOMI G'GGCC_C
Cuts at: 0 2440 2529
Size: 2440 89
PstI C_TGCA'G
Cuts at: 0 51 368 842 919 2198 2529
Size: 51 317 474 77 1279 331
Fragments arranged by size:
1279 474 331 317 77 51
PvuII CAG'CTG
Cuts at: 0 1351 1520 2019 2270 2529
Size: 1351 169 499 251 259
Fragments arranged by size:
1351 499 259 251 169
SacI G_AGCT'C
Cuts at: 0 128 2524 2529
Size: 128 2396 5
Fragments arranged by size:
2396 128 5
SanDI GG'GwC_CC
Cuts at: 0 134 1581 2529
Size: 134 1447 948
Fragments arranged by size:
1447 948 134
SapI GCTCTTCn'nnn_
Cuts at: 0 116 2529
Size: 116 2413
SfcI C'TryA_G
Cuts at: 0 47 364 838 915 1950 2194 2529
Size: 47 317 474 77 1035 244 335
Fragments arranged by size:
1035 474 335 317 244 77 47
SfoI GGC'GCC
Cuts at: 0 2137 2529
Size: 2137 392
SmaI CCC'GGG
Cuts at: 0 1789 2529
Size: 1789 740
SmlI C'TyrA_G
Cuts at: 0 936 1261 2529
Size: 936 325 1268
Fragments arranged by size:
1268 936 325
SrfI GCCC'GGGC
Cuts at: 0 1789 2529
Size: 1789 740
StuI AGG'CCT
Cuts at: 0 190 445 518 536 997 2529
Size: 190 255 73 18 461 1532
Fragments arranged by size:
1532 461 255 190 73 18
StyI C'CwwG_G
Cuts at: 0 1152 2529
Size: 1152 1377
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 611 685 1226 1606 2529
Size: 611 74 541 380 923
Fragments arranged by size:
923 611 541 380 74
TatI w'GTAC_w
Cuts at: 0 713 2529
Size: 713 1816
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 586 618 2171 2203 2529
Size: 586 32 1553 32 326
Fragments arranged by size:
1553 586 326 32 32
XbaI T'CTAG_A
Cuts at: 0 79 2529
Size: 79 2450
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2284 2355 2529
Size: 2284 71 174
Fragments arranged by size:
2284 174 71
XmaI C'CCGG_G
Cuts at: 0 1787 2529
Size: 1787 742
Enzymes that do cut:
AcuI AflIII AlfI AloI AlwNI ApaI ApoI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BciVI BfrBI
BglI BlpI Bme1580I BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspMI BsrBI
BsrDI BsrFI BssSI BstXI BstYI BtgI BtgZI BtsI
DraIII EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FspI HaeII Hin4I KasI MmeI MslI MspA1I
NaeI NarI NgoMIV NheI NsiI NspI PasI PflMI
PfoI PmlI PpiI PpuMI PshAI PspOMI PstI PvuII
SacI SanDI SapI SfcI SfoI SmaI SmlI SrfI
StuI StyI TaqII TatI TstI XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI ApaLI AscI AseI AsiSI AvrII BaeI
BcgI BclI BglII BmgBI BsaBI BsiEI BsiWI BspHI
BsrGI BssHII BstAPI BstBI BstEII BstZ17I Bsu36I ClaI
CspCI DraI DrdI EagI EcoRV FalI FseI FspAI
HincII HindIII HpaI KpnI MfeI MluI MscI NcoI
NdeI NotI NruI PacI PciI PmeI PsiI PspXI
PsrI PvuI RsrII SacII SalI SbfI ScaI SexAI
SfiI SgfI SgrAI SnaBI SpeI SphI SspI SwaI
Tth111I XhoI XmnI ZraI