Restriction map for
pF1KA1622
(Linear) (Six-base) MAPSORT of: KIAA1622.seq Check: 5471 from: 1 to: 2619
KIAA1622.orf
With 182 enzymes: SgfI *
October 24, 2006 15:08 ..
AccI GT'mk_AC
Cuts at: 0 1393 2619
Size: 1393 1226
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1244 1504 1918 1927 2619
Size: 1244 260 414 9 692
Fragments arranged by size:
1244 692 414 260 9
AflIII A'CryG_T
Cuts at: 0 1542 2619
Size: 1542 1077
AhdI GACnn_n'nnGTC
Cuts at: 0 1693 2619
Size: 1693 926
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1675 2619
Size: 1675 944
AlwNI CAG_nnn'CTG
Cuts at: 0 1468 2619
Size: 1468 1151
ApoI r'AATT_y
Cuts at: 0 584 607 977 1012 1281 1771 2619
Size: 584 23 370 35 269 490 848
Fragments arranged by size:
848 584 490 370 269 35 23
AseI AT'TA_AT
Cuts at: 0 766 1708 2619
Size: 766 942 911
Fragments arranged by size:
942 911 766
BanII G_rGCy'C
Cuts at: 0 80 2619
Size: 80 2539
BbsI GAAGACnn'nnnn_
Cuts at: 0 158 2619
Size: 158 2461
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1631 1665 2619
Size: 1631 34 954
Fragments arranged by size:
1631 954 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1755 2619
Size: 1755 864
BfrBI ATG'CAT
Cuts at: 0 3 385 454 1652 2619
Size: 3 382 69 1198 967
Fragments arranged by size:
1198 967 382 69 3
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 445 971 1307 1360 2619
Size: 445 526 336 53 1259
Fragments arranged by size:
1259 526 445 336 53
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 97 1020 2619
Size: 97 923 1599
Fragments arranged by size:
1599 923 97
BsaBI GATnn'nnATC
Cuts at: 0 1925 2619
Size: 1925 694
BsaWI w'CCGG_w
Cuts at: 0 120 2459 2619
Size: 120 2339 160
Fragments arranged by size:
2339 160 120
BsiHKAI G_wGCw'C
Cuts at: 0 80 431 758 2619
Size: 80 351 327 1861
Fragments arranged by size:
1861 351 327 80
BsmBI CGTCTCn'nnnn_
Cuts at: 0 256 2619
Size: 256 2363
Bsp1286I G_dGCh'C
Cuts at: 0 80 431 758 2619
Size: 80 351 327 1861
Fragments arranged by size:
1861 351 327 80
BspEI T'CCGG_A
Cuts at: 0 2459 2619
Size: 2459 160
BspHI T'CATG_A
Cuts at: 0 1506 1598 2619
Size: 1506 92 1021
Fragments arranged by size:
1506 1021 92
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 77 1833 2619
Size: 77 1756 786
Fragments arranged by size:
1756 786 77
BsrDI GCAATG_nn'
Cuts at: 0 456 2619
Size: 456 2163
BsrFI r'CCGG_y
Cuts at: 0 96 2619
Size: 96 2523
BsrGI T'GTAC_A
Cuts at: 0 1030 2619
Size: 1030 1589
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2226 2619
Size: 2226 393
BstBI TT'CG_AA
Cuts at: 0 2313 2619
Size: 2313 306
BstXI CCAn_nnnn'nTGG
Cuts at: 0 727 2619
Size: 727 1892
BtgI C'CryG_G
Cuts at: 0 2526 2581 2619
Size: 2526 55 38
Fragments arranged by size:
2526 55 38
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 40 352 2619
Size: 40 312 2267
Fragments arranged by size:
2267 312 40
BtsI GCAGTG_nn'
Cuts at: 0 801 1416 2565 2619
Size: 801 615 1149 54
Fragments arranged by size:
1149 801 615 54
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 731 766 2541 2576 2619
Size: 731 35 1775 35 43
Fragments arranged by size:
1775 731 43 35 35
DraI TTT'AAA
Cuts at: 0 1493 2619
Size: 1493 1126
DrdI GACnn_nn'nnGTC
Cuts at: 0 442 2619
Size: 442 2177
EaeI y'GGCC_r
Cuts at: 0 1145 2619
Size: 1145 1474
EarI CTCTTCn'nnn_
Cuts at: 0 473 2268 2619
Size: 473 1795 351
Fragments arranged by size:
1795 473 351
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 91 2619
Size: 91 2528
EcoICRI GAG'CTC
Cuts at: 0 78 2619
Size: 78 2541
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 445 971 1244 1307 1360 1504 1918 1927
Size: 445 526 273 63 53 144 414 9
Cuts at: 1927 2619
Size: 692
Fragments arranged by size:
692 526 445 414 273 144 63 53
9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1093 2619
Size: 1093 1526
EcoO109I rG'GnC_Cy
Cuts at: 0 66 2619
Size: 66 2553
EcoRI G'AATT_C
Cuts at: 0 1281 2619
Size: 1281 1338
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2128 2339 2610 2619
Size: 2128 211 271 9
Fragments arranged by size:
2128 271 211 9
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 128 160 213 245 507 539 1679 1711
Size: 128 32 53 32 262 32 1140 32
Cuts at: 1711 1830 1862 2619
Size: 119 32 757
Fragments arranged by size:
1140 757 262 128 119 53 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 329 2547 2619
Size: 329 2218 72
Fragments arranged by size:
2218 329 72
HindIII A'AGCT_T
Cuts at: 0 1273 2336 2619
Size: 1273 1063 283
Fragments arranged by size:
1273 1063 283
HpaI GTT'AAC
Cuts at: 0 329 2619
Size: 329 2290
MfeI C'AATT_G
Cuts at: 0 1717 2619
Size: 1717 902
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2003 2063 2619
Size: 2003 60 556
Fragments arranged by size:
2003 556 60
MslI CAynn'nnrTG
Cuts at: 0 1597 2619
Size: 1597 1022
MspA1I CmG'CkG
Cuts at: 0 1465 2619
Size: 1465 1154
NsiI A_TGCA'T
Cuts at: 0 5 387 456 1654 2619
Size: 5 382 69 1198 965
Fragments arranged by size:
1198 965 382 69 5
NspI r_CATG'y
Cuts at: 0 307 1546 2619
Size: 307 1239 1073
Fragments arranged by size:
1239 1073 307
PasI CC'CwG_GG
Cuts at: 0 720 2619
Size: 720 1899
PciI A'CATG_T
Cuts at: 0 1542 2619
Size: 1542 1077
PfoI T'CCnGG_A
Cuts at: 0 2253 2619
Size: 2253 366
PpuMI rG'GwC_Cy
Cuts at: 0 66 2619
Size: 66 2553
PshAI GACnn'nnGTC
Cuts at: 0 860 2619
Size: 860 1759
PsiI TTA'TAA
Cuts at: 0 1019 1136 1667 2619
Size: 1019 117 531 952
Fragments arranged by size:
1019 952 531 117
PstI C_TGCA'G
Cuts at: 0 74 362 1485 2619
Size: 74 288 1123 1134
Fragments arranged by size:
1134 1123 288 74
PvuII CAG'CTG
Cuts at: 0 1465 2619
Size: 1465 1154
RsrII CG'GwC_CG
Cuts at: 0 99 2619
Size: 99 2520
SacI G_AGCT'C
Cuts at: 0 80 2619
Size: 80 2539
SbfI CC_TGCA'GG
Cuts at: 0 74 2619
Size: 74 2545
ScaI AGT'ACT
Cuts at: 0 2520 2619
Size: 2520 99
SfcI C'TryA_G
Cuts at: 0 70 358 1481 2619
Size: 70 288 1123 1138
Fragments arranged by size:
1138 1123 288 70
SmlI C'TyrA_G
Cuts at: 0 112 999 2619
Size: 112 887 1620
Fragments arranged by size:
1620 887 112
SspI AAT'ATT
Cuts at: 0 1813 2619
Size: 1813 806
StyI C'CwwG_G
Cuts at: 0 207 536 1725 2619
Size: 207 329 1189 894
Fragments arranged by size:
1189 894 329 207
TatI w'GTAC_w
Cuts at: 0 1030 2518 2619
Size: 1030 1488 101
Fragments arranged by size:
1488 1030 101
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 203 235 1070 1102 1113 1145 2619
Size: 203 32 835 32 11 32 1474
Fragments arranged by size:
1474 835 203 32 32 32 11
XmnI GAAnn'nnTTC
Cuts at: 0 467 638 923 2111 2427 2619
Size: 467 171 285 1188 316 192
Fragments arranged by size:
1188 467 316 285 192 171
Enzymes that do cut:
AccI AcuI AflIII AhdI AlfI AlwNI ApoI AseI
BanII BbsI BcgI BciVI BfrBI BpmI BpuEI BsaBI
BsaWI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrDI
BsrFI BsrGI BstAPI BstBI BstXI BtgI BtgZI BtsI
CspCI DraI DrdI EaeI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI Hin4I HincII HindIII HpaI
MfeI MmeI MslI MspA1I NsiI NspI PasI PciI
PfoI PpuMI PshAI PsiI PstI PvuII RsrII SacI
SbfI ScaI SfcI SmlI SspI StyI TatI TstI
XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AleI
AloI ApaI ApaLI AscI AsiSI AvaI AvrII BaeI
BamHI BanI BbeI BbvCI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI Bpu10I BsaI BsaAI
BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiWI BsmI
BsrBI BssHII BssSI BstEII BstYI BstZ17I Bsu36I ClaI
DraIII EagI EcoRV FseI FspI FspAI HaeII KasI
KpnI MluI MscI NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI PacI PflMI PmeI PmlI PpiI
PspOMI PspXI PsrI PvuI SacII SalI SanDI SapI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI StuI SwaI TaqII Tth111I XbaI XcmI
XhoI XmaI ZraI