Restriction map for
pF1KA1625
(Linear) (Six-base) MAPSORT of: KIAA1625.seq Check: 2071 from: 1 to: 2271
KIAA1625.orf
With 182 enzymes: SgfI *
October 24, 2006 15:09 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 75 604 1156 1879 1966 2271
Size: 75 529 552 723 87 305
Fragments arranged by size:
723 552 529 305 87 75
AflIII A'CryG_T
Cuts at: 0 1412 2271
Size: 1412 859
AgeI A'CCGG_T
Cuts at: 0 556 2271
Size: 556 1715
AhdI GACnn_n'nnGTC
Cuts at: 0 1455 1929 2271
Size: 1455 474 342
Fragments arranged by size:
1455 474 342
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1744 1990 2271
Size: 1744 246 281
Fragments arranged by size:
1744 281 246
AlwNI CAG_nnn'CTG
Cuts at: 0 376 955 1065 1143 1554 2271
Size: 376 579 110 78 411 717
Fragments arranged by size:
717 579 411 376 110 78
ApaI G_GGCC'C
Cuts at: 0 2111 2271
Size: 2111 160
ApoI r'AATT_y
Cuts at: 0 254 850 1291 2271
Size: 254 596 441 980
Fragments arranged by size:
980 596 441 254
AvaI C'yCGr_G
Cuts at: 0 10 810 2271
Size: 10 800 1461
Fragments arranged by size:
1461 800 10
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 327 360 2271
Size: 327 33 1911
Fragments arranged by size:
1911 327 33
BamHI G'GATC_C
Cuts at: 0 1622 1691 2271
Size: 1622 69 580
Fragments arranged by size:
1622 580 69
BanII G_rGCy'C
Cuts at: 0 881 1055 1087 1385 1841 2111 2271
Size: 881 174 32 298 456 270 160
Fragments arranged by size:
881 456 298 270 174 160 32
BbsI GAAGACnn'nnnn_
Cuts at: 0 497 2271
Size: 497 1774
BciVI GTATCCnnnnn_n'
Cuts at: 0 328 791 2271
Size: 328 463 1480
Fragments arranged by size:
1480 463 328
BclI T'GATC_A
Cuts at: 0 1889 2271
Size: 1889 382
BglI GCCn_nnn'nGGC
Cuts at: 0 507 2271
Size: 507 1764
BglII A'GATC_T
Cuts at: 0 1128 1578 2271
Size: 1128 450 693
Fragments arranged by size:
1128 693 450
BlpI GC'TnA_GC
Cuts at: 0 514 2271
Size: 514 1757
Bme1580I G_kGCm'C
Cuts at: 0 1538 2111 2271
Size: 1538 573 160
Fragments arranged by size:
1538 573 160
BmrI ACTGGGnnnn_n'
Cuts at: 0 990 1070 1800 2054 2271
Size: 990 80 730 254 217
Fragments arranged by size:
990 730 254 217 80
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2080 2271
Size: 2080 191
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 15 590 791 1111 1800 1903 2271
Size: 15 575 201 320 689 103 368
Fragments arranged by size:
689 575 368 320 201 103 15
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1030 2271
Size: 1030 1241
BsaI GGTCTCn'nnnn_
Cuts at: 0 878 2242 2271
Size: 878 1364 29
Fragments arranged by size:
1364 878 29
BsaAI yAC'GTr
Cuts at: 0 782 2271
Size: 782 1489
BsaHI Gr'CG_yC
Cuts at: 0 667 2271
Size: 667 1604
BsaWI w'CCGG_w
Cuts at: 0 331 556 1428 1763 2180 2271
Size: 331 225 872 335 417 91
Fragments arranged by size:
872 417 335 331 225 91
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 587 1114 1243 1756 2260 2271
Size: 587 527 129 513 504 11
Fragments arranged by size:
587 527 513 504 129 11
BseYI C'CCAG_C
Cuts at: 0 932 1054 1554 2271
Size: 932 122 500 717
Fragments arranged by size:
932 717 500 122
BsiEI CG_ry'CG
Cuts at: 0 1261 2271
Size: 1261 1010
BsiHKAI G_wGCw'C
Cuts at: 0 1841 2271
Size: 1841 430
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1993 2271
Size: 1993 278
Bsp1286I G_dGCh'C
Cuts at: 0 881 1055 1087 1385 1538 1841 2111 2271
Size: 881 174 32 298 153 303 270 160
Fragments arranged by size:
881 303 298 270 174 160 153 32
BspEI T'CCGG_A
Cuts at: 0 1428 2271
Size: 1428 843
BspHI T'CATG_A
Cuts at: 0 1025 2271
Size: 1025 1246
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 575 2179 2260 2271
Size: 575 1604 81 11
Fragments arranged by size:
1604 575 81 11
BsrBI CCG'CTC
Cuts at: 0 513 2064 2271
Size: 513 1551 207
Fragments arranged by size:
1551 513 207
BsrDI GCAATG_nn'
Cuts at: 0 1191 2271
Size: 1191 1080
BsrFI r'CCGG_y
Cuts at: 0 433 556 2271
Size: 433 123 1715
Fragments arranged by size:
1715 433 123
BssSI C'ACGA_G
Cuts at: 0 416 2271
Size: 416 1855
BstBI TT'CG_AA
Cuts at: 0 636 2271
Size: 636 1635
BstYI r'GATC_y
Cuts at: 0 41 348 1128 1578 1622 1691 2271
Size: 41 307 780 450 44 69 580
Fragments arranged by size:
780 580 450 307 69 44 41
BtgI C'CryG_G
Cuts at: 0 1296 1503 2240 2271
Size: 1296 207 737 31
Fragments arranged by size:
1296 737 207 31
BtsI GCAGTG_nn'
Cuts at: 0 145 919 1069 2271
Size: 145 774 150 1202
Fragments arranged by size:
1202 774 150 145
DraIII CAC_nnn'GTG
Cuts at: 0 1473 1956 2271
Size: 1473 483 315
Fragments arranged by size:
1473 483 315
EaeI y'GGCC_r
Cuts at: 0 390 687 1328 1958 2271
Size: 390 297 641 630 313
Fragments arranged by size:
641 630 390 313 297
EarI CTCTTCn'nnn_
Cuts at: 0 1390 2271
Size: 1390 881
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1494 1704 2271
Size: 1494 210 567
Fragments arranged by size:
1494 567 210
EcoICRI GAG'CTC
Cuts at: 0 1839 2271
Size: 1839 432
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 15 75 590 604 791 1111 1156 1800
Size: 15 60 515 14 187 320 45 644
Cuts at: 1800 1879 1903 1966 2271
Size: 79 24 63 305
Fragments arranged by size:
644 515 320 305 187 79 63 60
45 24 15 14
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2158 2271
Size: 2158 113
EcoO109I rG'GnC_Cy
Cuts at: 0 2107 2108 2271
Size: 2107 1 163
Fragments arranged by size:
2107 163 1
EcoRI G'AATT_C
Cuts at: 0 850 2271
Size: 850 1421
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 102 2271
Size: 102 2169
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 789 821 1118 1150 1205 1237 1441 1473
Size: 789 32 297 32 55 32 204 32
Cuts at: 1473 1499 1531 1570 1602 1915 1947 2271
Size: 26 32 39 32 313 32 324
Fragments arranged by size:
789 324 313 297 204 55 39 32
32 32 32 32 32 32 26
MscI TGG'CCA
Cuts at: 0 392 1330 2271
Size: 392 938 941
Fragments arranged by size:
941 938 392
MslI CAynn'nnrTG
Cuts at: 0 235 1204 2271
Size: 235 969 1067
Fragments arranged by size:
1067 969 235
MspA1I CmG'CkG
Cuts at: 0 518 630 1065 1551 2271
Size: 518 112 435 486 720
Fragments arranged by size:
720 518 486 435 112
NcoI C'CATG_G
Cuts at: 0 1503 2271
Size: 1503 768
NdeI CA'TA_TG
Cuts at: 0 2199 2271
Size: 2199 72
NspI r_CATG'y
Cuts at: 0 1416 2271
Size: 1416 855
PciI A'CATG_T
Cuts at: 0 1412 2271
Size: 1412 859
PflMI CCAn_nnn'nTGG
Cuts at: 0 345 399 1337 1473 2271
Size: 345 54 938 136 798
Fragments arranged by size:
938 798 345 136 54
PmlI CAC'GTG
Cuts at: 0 782 2271
Size: 782 1489
PspOMI G'GGCC_C
Cuts at: 0 2107 2271
Size: 2107 164
PstI C_TGCA'G
Cuts at: 0 457 1700 1823 2036 2271
Size: 457 1243 123 213 235
Fragments arranged by size:
1243 457 235 213 123
PvuII CAG'CTG
Cuts at: 0 518 1551 2271
Size: 518 1033 720
Fragments arranged by size:
1033 720 518
SacI G_AGCT'C
Cuts at: 0 1841 2271
Size: 1841 430
SapI GCTCTTCn'nnn_
Cuts at: 0 1390 2271
Size: 1390 881
SfcI C'TryA_G
Cuts at: 0 453 661 1696 1819 2032 2093 2271
Size: 453 208 1035 123 213 61 178
Fragments arranged by size:
1035 453 213 208 178 123 61
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 507 2271
Size: 507 1764
SgrAI Cr'CCGG_yG
Cuts at: 0 556 2271
Size: 556 1715
SmaI CCC'GGG
Cuts at: 0 12 2271
Size: 12 2259
SmlI C'TyrA_G
Cuts at: 0 1045 2271
Size: 1045 1226
SspI AAT'ATT
Cuts at: 0 1407 2271
Size: 1407 864
StyI C'CwwG_G
Cuts at: 0 1009 1503 2067 2271
Size: 1009 494 564 204
Fragments arranged by size:
1009 564 494 204
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1275 2271
Size: 1275 996
TatI w'GTAC_w
Cuts at: 0 946 1647 2271
Size: 946 701 624
Fragments arranged by size:
946 701 624
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 166 198 2271
Size: 166 32 2073
Fragments arranged by size:
2073 166 32
Tth111I GACn'n_nGTC
Cuts at: 0 2046 2271
Size: 2046 225
XmaI C'CCGG_G
Cuts at: 0 10 2271
Size: 10 2261
XmnI GAAnn'nnTTC
Cuts at: 0 85 854 1828 2271
Size: 85 769 974 443
Fragments arranged by size:
974 769 443 85
Enzymes that do cut:
AcuI AflIII AgeI AhdI AlfI AlwNI ApaI ApoI
AvaI BaeI BamHI BanII BbsI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI
BsaAI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstBI BstYI BtgI BtsI DraIII EaeI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI Hin4I MscI
MslI MspA1I NcoI NdeI NspI PciI PflMI PmlI
PspOMI PstI PvuII SacI SapI SfcI SfiI SgrAI
SmaI SmlI SspI StyI TaqII TatI TstI Tth111I
XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AleI
AloI ApaLI AscI AseI AsiSI AvrII BanI BbeI
BbvCI BcgI BfrBI BmgBI BmtI Bpu10I BsaBI BsaXI
BsgI BsiWI BsmI BsrGI BssHII BstAPI BstEII BstXI
BstZ17I Bsu36I BtgZI ClaI CspCI DraI DrdI EagI
EcoRV FseI FspI FspAI HaeII HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI NaeI NarI NgoMIV
NheI NotI NruI NsiI PacI PasI PfoI PmeI
PpiI PpuMI PshAI PsiI PspXI PsrI PvuI RsrII
SacII SalI SanDI SbfI ScaI SexAI SfoI SgfI
SnaBI SpeI SphI SrfI StuI SwaI XbaI XcmI
XhoI ZraI