Restriction map for
pF1KA1628
(Linear) (Six-base) MAPSORT of: KIAA1628.seq Check: 4191 from: 1 to: 3750
KIAA1628.orf
With 182 enzymes: SgfI *
April 24, 2009 13:00 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 266 1662 3750
Size: 266 1396 2088
Fragments arranged by size:
2088 1396 266
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 574 648 1756 2343 2700 3140 3173 3413
Size: 574 74 1108 587 357 440 33 240
Cuts at: 3413 3750
Size: 337
Fragments arranged by size:
1108 587 574 440 357 337 240 74
33
AfeI AGC'GCT
Cuts at: 0 1094 2518 3750
Size: 1094 1424 1232
Fragments arranged by size:
1424 1232 1094
AleI CACnn'nnGTG
Cuts at: 0 205 760 763 2782 3750
Size: 205 555 3 2019 968
Fragments arranged by size:
2019 968 555 205 3
AlwNI CAG_nnn'CTG
Cuts at: 0 325 597 979 2366 3306 3321 3750
Size: 325 272 382 1387 940 15 429
Fragments arranged by size:
1387 940 429 382 325 272 15
ApaI G_GGCC'C
Cuts at: 0 145 1833 3276 3750
Size: 145 1688 1443 474
Fragments arranged by size:
1688 1443 474 145
ApaLI G'TGCA_C
Cuts at: 0 1558 3064 3124 3750
Size: 1558 1506 60 626
Fragments arranged by size:
1558 1506 626 60
AvaI C'yCGr_G
Cuts at: 0 1021 1354 2986 3269 3501 3574 3750
Size: 1021 333 1632 283 232 73 176
Fragments arranged by size:
1632 1021 333 283 232 176 73
BamHI G'GATC_C
Cuts at: 0 3513 3750
Size: 3513 237
BanI G'GyrC_C
Cuts at: 0 1017 1116 2211 2217 2603 3750
Size: 1017 99 1095 6 386 1147
Fragments arranged by size:
1147 1095 1017 386 99 6
BanII G_rGCy'C
Cuts at: 0 35 145 986 1833 2375 2572 2621 3015
Size: 35 110 841 847 542 197 49 394
Cuts at: 3015 3192 3276 3750
Size: 177 84 474
Fragments arranged by size:
847 841 542 474 394 197 177 110
84 49 35
BbeI G_GCGC'C
Cuts at: 0 1021 1120 2215 3750
Size: 1021 99 1095 1535
Fragments arranged by size:
1535 1095 1021 99
BbsI GAAGACnn'nnnn_
Cuts at: 0 2423 3750
Size: 2423 1327
BbvCI CC'TCA_GC
Cuts at: 0 801 2009 2723 3750
Size: 801 1208 714 1027
Fragments arranged by size:
1208 1027 801 714
BclI T'GATC_A
Cuts at: 0 2819 3750
Size: 2819 931
BfrBI ATG'CAT
Cuts at: 0 1848 3750
Size: 1848 1902
BglI GCCn_nnn'nGGC
Cuts at: 0 1125 1134 2079 2535 3750
Size: 1125 9 945 456 1215
Fragments arranged by size:
1215 1125 945 456 9
BglII A'GATC_T
Cuts at: 0 1970 3179 3750
Size: 1970 1209 571
Fragments arranged by size:
1970 1209 571
BlpI GC'TnA_GC
Cuts at: 0 106 980 3205 3750
Size: 106 874 2225 545
Fragments arranged by size:
2225 874 545 106
Bme1580I G_kGCm'C
Cuts at: 0 145 1562 1833 2608 3068 3128 3276 3750
Size: 145 1417 271 775 460 60 148 474
Fragments arranged by size:
1417 775 474 460 271 148 145 60
BmgBI CAC'GTC
Cuts at: 0 729 2265 3105 3750
Size: 729 1536 840 645
Fragments arranged by size:
1536 840 729 645
BmrI ACTGGGnnnn_n'
Cuts at: 0 1574 1833 1856 3305 3750
Size: 1574 259 23 1449 445
Fragments arranged by size:
1574 1449 445 259 23
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2765 3750
Size: 2765 985
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 234 259 2240 2432 2758 2812 2991 3464
Size: 234 25 1981 192 326 54 179 473
Cuts at: 3464 3478 3622 3689 3750
Size: 14 144 67 61
Fragments arranged by size:
1981 473 326 234 192 179 144 67
61 54 25 14
Bpu10I CC'TnA_GC
Cuts at: 0 801 1660 2009 2374 2675 2723 3750
Size: 801 859 349 365 301 48 1027
Fragments arranged by size:
1027 859 801 365 349 301 48
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2068 3750
Size: 2068 1682
BsaI GGTCTCn'nnnn_
Cuts at: 0 2045 3655 3741 3750
Size: 2045 1610 86 9
Fragments arranged by size:
2045 1610 86 9
BsaAI yAC'GTr
Cuts at: 0 1506 3750
Size: 1506 2244
BsaHI Gr'CG_yC
Cuts at: 0 14 584 709 923 1018 1117 1196 1858
Size: 14 570 125 214 95 99 79 662
Cuts at: 1858 2212 3332 3750
Size: 354 1120 418
Fragments arranged by size:
1120 662 570 418 354 214 125 99
95 79 14
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 64 94 2524 2554 2824 2854 3750
Size: 64 30 2430 30 270 30 896
Fragments arranged by size:
2430 896 270 64 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 25 478 568 2026 2029 2890 3673 3729
Size: 25 453 90 1458 3 861 783 56
Cuts at: 3729 3732 3750
Size: 3 18
Fragments arranged by size:
1458 861 783 453 90 56 25 18
3 3
BseYI C'CCAG_C
Cuts at: 0 287 1285 1520 1524 1548 1592 1654 2114
Size: 287 998 235 4 24 44 62 460
Cuts at: 2114 3028 3036 3209 3366 3750
Size: 914 8 173 157 384
Fragments arranged by size:
998 914 460 384 287 235 173 157
62 44 24 8 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 424 1090 1604 1933 2488 2581 3750
Size: 424 666 514 329 555 93 1169
Fragments arranged by size:
1169 666 555 514 424 329 93
BsiEI CG_ry'CG
Cuts at: 0 21 27 2031 3259 3750
Size: 21 6 2004 1228 491
Fragments arranged by size:
2004 1228 491 21 6
BsiHKAI G_wGCw'C
Cuts at: 0 245 986 1051 1562 2681 3015 3068 3128
Size: 245 741 65 511 1119 334 53 60
Cuts at: 3128 3750
Size: 622
Fragments arranged by size:
1119 741 622 511 334 245 65 60
53
BsmI GAATG_Cn'
Cuts at: 0 2385 3750
Size: 2385 1365
BsmBI CGTCTCn'nnnn_
Cuts at: 0 85 3750
Size: 85 3665
Bsp1286I G_dGCh'C
Cuts at: 0 35 145 245 986 1051 1562 1833 2375
Size: 35 110 100 741 65 511 271 542
Cuts at: 2375 2572 2608 2621 2681 3015 3068 3128 3192
Size: 197 36 13 60 334 53 60 64
Cuts at: 3192 3276 3750
Size: 84 474
Fragments arranged by size:
741 542 511 474 334 271 197 110
100 84 65 64 60 60 53 36
35 13
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 266 1662 2567 3601 3750
Size: 266 1396 905 1034 149
Fragments arranged by size:
1396 1034 905 266 149
BsrBI CCG'CTC
Cuts at: 0 1350 3750
Size: 1350 2400
BsrDI GCAATG_nn'
Cuts at: 0 544 1671 3351 3750
Size: 544 1127 1680 399
Fragments arranged by size:
1680 1127 544 399
BsrFI r'CCGG_y
Cuts at: 0 16 1198 1819 2129 2214 2534 3258 3750
Size: 16 1182 621 310 85 320 724 492
Fragments arranged by size:
1182 724 621 492 320 310 85 16
BsrGI T'GTAC_A
Cuts at: 0 2651 3750
Size: 2651 1099
BssHII G'CGCG_C
Cuts at: 0 64 1054 2773 3750
Size: 64 990 1719 977
Fragments arranged by size:
1719 990 977 64
BssSI C'ACGA_G
Cuts at: 0 2149 3750
Size: 2149 1601
BstAPI GCAn_nnn'nTGC
Cuts at: 0 3613 3750
Size: 3613 137
BstEII G'GTnAC_C
Cuts at: 0 207 2391 3750
Size: 207 2184 1359
Fragments arranged by size:
2184 1359 207
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1975 3750
Size: 1975 1775
BstYI r'GATC_y
Cuts at: 0 593 1970 3179 3513 3727 3750
Size: 593 1377 1209 334 214 23
Fragments arranged by size:
1377 1209 593 334 214 23
Bsu36I CC'TnA_GG
Cuts at: 0 326 551 3464 3750
Size: 326 225 2913 286
Fragments arranged by size:
2913 326 286 225
BtgI C'CryG_G
Cuts at: 0 21 27 369 1084 1262 2046 2585 2730
Size: 21 6 342 715 178 784 539 145
Cuts at: 2730 3750
Size: 1020
Fragments arranged by size:
1020 784 715 539 342 178 145 21
6
BtsI GCAGTG_nn'
Cuts at: 0 124 164 315 968 1304 1445 2192 2471
Size: 124 40 151 653 336 141 747 279
Cuts at: 2471 3750
Size: 1279
Fragments arranged by size:
1279 747 653 336 279 151 141 124
40
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 801 836 3750
Size: 801 35 2914
Fragments arranged by size:
2914 801 35
DraIII CAC_nnn'GTG
Cuts at: 0 2481 3750
Size: 2481 1269
DrdI GACnn_nn'nnGTC
Cuts at: 0 319 3750
Size: 319 3431
EaeI y'GGCC_r
Cuts at: 0 18 24 59 389 638 771 1087 1259
Size: 18 6 35 330 249 133 316 172
Cuts at: 1259 2127 3256 3750
Size: 868 1129 494
Fragments arranged by size:
1129 868 494 330 316 249 172 133
35 18 6
EagI C'GGCC_G
Cuts at: 0 18 24 3256 3750
Size: 18 6 3232 494
Fragments arranged by size:
3232 494 18 6
EcoICRI GAG'CTC
Cuts at: 0 984 3013 3750
Size: 984 2029 737
Fragments arranged by size:
2029 984 737
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 234 259 574 648 1756 2240 2343 2432
Size: 234 25 315 74 1108 484 103 89
Cuts at: 2432 2700 2758 2812 2991 3140 3173 3413 3464
Size: 268 58 54 179 149 33 240 51
Cuts at: 3464 3478 3622 3689 3750
Size: 14 144 67 61
Fragments arranged by size:
1108 484 315 268 240 234 179 149
144 103 89 74 67 61 58 54
51 33 25 14
EcoO109I rG'GnC_Cy
Cuts at: 0 622 675 701 1473 1829 1830 2071 2788
Size: 622 53 26 772 356 1 241 717
Cuts at: 2788 3250 3273 3468 3750
Size: 462 23 195 282
Fragments arranged by size:
772 717 622 462 356 282 241 195
53 26 23 1
HaeII r_GCGC'y
Cuts at: 0 1021 1030 1096 1120 1292 2215 2520 3750
Size: 1021 9 66 24 172 923 305 1230
Fragments arranged by size:
1230 1021 923 305 172 66 24 9
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1015 1047 2405 2437 2524 2556 2824 2845
Size: 1015 32 1358 32 87 32 268 21
Cuts at: 2845 2856 2877 3484 3516 3719 3750
Size: 11 21 607 32 203 31
Fragments arranged by size:
1358 1015 607 268 203 87 32 32
32 32 31 21 21 11
HincII GTy'rAC
Cuts at: 0 44 2343 3750
Size: 44 2299 1407
Fragments arranged by size:
2299 1407 44
HindIII A'AGCT_T
Cuts at: 0 412 3750
Size: 412 3338
KasI G'GCGC_C
Cuts at: 0 1017 1116 2211 3750
Size: 1017 99 1095 1539
Fragments arranged by size:
1539 1095 1017 99
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 858 2579 3750
Size: 858 1721 1171
Fragments arranged by size:
1721 1171 858
MscI TGG'CCA
Cuts at: 0 391 640 773 3750
Size: 391 249 133 2977
Fragments arranged by size:
2977 391 249 133
MslI CAynn'nnrTG
Cuts at: 0 205 760 763 1630 2171 2267 2782 2878
Size: 205 555 3 867 541 96 515 96
Cuts at: 2878 3750
Size: 872
Fragments arranged by size:
872 867 555 541 515 205 96 96
3
MspA1I CmG'CkG
Cuts at: 0 23 190 632 805 979 1086 1122 1330
Size: 23 167 442 173 174 107 36 208
Cuts at: 1330 1524 3040 3750
Size: 194 1516 710
Fragments arranged by size:
1516 710 442 208 194 174 173 167
107 36 23
NaeI GCC'GGC
Cuts at: 0 18 1200 1821 2131 2216 3260 3750
Size: 18 1182 621 310 85 1044 490
Fragments arranged by size:
1182 1044 621 490 310 85 18
NarI GG'CG_CC
Cuts at: 0 1018 1117 2212 3750
Size: 1018 99 1095 1538
Fragments arranged by size:
1538 1095 1018 99
NcoI C'CATG_G
Cuts at: 0 2730 3750
Size: 2730 1020
NgoMIV G'CCGG_C
Cuts at: 0 16 1198 1819 2129 2214 3258 3750
Size: 16 1182 621 310 85 1044 492
Fragments arranged by size:
1182 1044 621 492 310 85 16
NsiI A_TGCA'T
Cuts at: 0 1850 3750
Size: 1850 1900
NspI r_CATG'y
Cuts at: 0 2229 2859 2922 3750
Size: 2229 630 63 828
Fragments arranged by size:
2229 828 630 63
PasI CC'CwG_GG
Cuts at: 0 2366 3750
Size: 2366 1384
PflMI CCAn_nnn'nTGG
Cuts at: 0 780 1215 2736 3051 3456 3750
Size: 780 435 1521 315 405 294
Fragments arranged by size:
1521 780 435 405 315 294
PfoI T'CCnGG_A
Cuts at: 0 596 2828 3750
Size: 596 2232 922
Fragments arranged by size:
2232 922 596
PpuMI rG'GwC_Cy
Cuts at: 0 701 1473 2788 3468 3750
Size: 701 772 1315 680 282
Fragments arranged by size:
1315 772 701 680 282
PshAI GACnn'nnGTC
Cuts at: 0 403 3750
Size: 403 3347
PspOMI G'GGCC_C
Cuts at: 0 141 1829 3272 3750
Size: 141 1688 1443 478
Fragments arranged by size:
1688 1443 478 141
PstI C_TGCA'G
Cuts at: 0 159 975 1193 1902 2813 3317 3376 3750
Size: 159 816 218 709 911 504 59 374
Fragments arranged by size:
911 816 709 504 374 218 159 59
PvuI CG_AT'CG
Cuts at: 0 2031 3750
Size: 2031 1719
PvuII CAG'CTG
Cuts at: 0 805 1524 3040 3750
Size: 805 719 1516 710
Fragments arranged by size:
1516 805 719 710
SacI G_AGCT'C
Cuts at: 0 986 3015 3750
Size: 986 2029 735
Fragments arranged by size:
2029 986 735
SacII CC_GC'GG
Cuts at: 0 24 1087 3750
Size: 24 1063 2663
Fragments arranged by size:
2663 1063 24
SanDI GG'GwC_CC
Cuts at: 0 701 3750
Size: 701 3049
SbfI CC_TGCA'GG
Cuts at: 0 1193 2813 3376 3750
Size: 1193 1620 563 374
Fragments arranged by size:
1620 1193 563 374
SexAI A'CCwGG_T
Cuts at: 0 536 3380 3750
Size: 536 2844 370
Fragments arranged by size:
2844 536 370
SfcI C'TryA_G
Cuts at: 0 155 168 971 1189 1462 1898 2809 3313
Size: 155 13 803 218 273 436 911 504
Cuts at: 3313 3372 3750
Size: 59 378
Fragments arranged by size:
911 803 504 436 378 273 218 155
59 13
SfoI GGC'GCC
Cuts at: 0 1019 1118 2213 3750
Size: 1019 99 1095 1537
Fragments arranged by size:
1537 1095 1019 99
SmaI CCC'GGG
Cuts at: 0 2988 3271 3576 3750
Size: 2988 283 305 174
Fragments arranged by size:
2988 305 283 174
SmlI C'TyrA_G
Cuts at: 0 2083 3750
Size: 2083 1667
StuI AGG'CCT
Cuts at: 0 2966 3719 3750
Size: 2966 753 31
Fragments arranged by size:
2966 753 31
StyI C'CwwG_G
Cuts at: 0 2730 3750
Size: 2730 1020
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 315 1981 2872 3127 3750
Size: 315 1666 891 255 623
Fragments arranged by size:
1666 891 623 315 255
TatI w'GTAC_w
Cuts at: 0 1682 2651 3002 3750
Size: 1682 969 351 748
Fragments arranged by size:
1682 969 748 351
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 685 717 2938 2970 3750
Size: 685 32 2221 32 780
Fragments arranged by size:
2221 780 685 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 730 2580 3750
Size: 730 1850 1170
Fragments arranged by size:
1850 1170 730
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1097 1156 1551 2122 3750
Size: 1097 59 395 571 1628
Fragments arranged by size:
1628 1097 571 395 59
XmaI C'CCGG_G
Cuts at: 0 2986 3269 3574 3750
Size: 2986 283 305 176
Fragments arranged by size:
2986 305 283 176
XmnI GAAnn'nnTTC
Cuts at: 0 2443 3750
Size: 2443 1307
Enzymes that do cut:
AarI AcuI AfeI AleI AlwNI ApaI ApaLI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BclI BfrBI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaHI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstEII BstXI
BstYI Bsu36I BtgI BtsI CspCI DraIII DrdI EaeI
EagI EcoICRI Eco57MI EcoO109I HaeII Hin4I HincII HindIII
KasI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NsiI NspI PasI PflMI PfoI PpuMI PshAI
PspOMI PstI PvuI PvuII SacI SacII SanDI SbfI
SexAI SfcI SfoI SmaI SmlI StuI StyI TaqII
TatI TstI Tth111I XcmI XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AflIII AgeI AhdI
AlfI AloI ApoI AscI AseI AsiSI AvrII BaeI
BcgI BciVI BmtI BsaBI BsaWI BsiWI BspEI BspHI
BstBI BstZ17I BtgZI ClaI DraI EarI EciI EcoNI
EcoRI EcoRV FalI FseI FspI FspAI HpaI KpnI
MfeI MluI NdeI NheI NotI NruI PacI PciI
PmeI PmlI PpiI PsiI PspXI PsrI RsrII SalI
SapI ScaI SfiI SgfI SgrAI SnaBI SpeI SphI
SrfI SspI SwaI XbaI XhoI ZraI