Restriction map for
pF1KA1645
(Linear) (Six-base) MAPSORT of: KIAA1645.seq Check: 2980 from: 1 to: 981
KIAA1645.orf
With 182 enzymes: SgfI *
October 24, 2006 15:09 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 720 981
Size: 720 261
AatII G_ACGT'C
Cuts at: 0 448 584 981
Size: 448 136 397
Fragments arranged by size:
448 397 136
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 289 481 981
Size: 289 192 500
Fragments arranged by size:
500 289 192
AfeI AGC'GCT
Cuts at: 0 306 981
Size: 306 675
AgeI A'CCGG_T
Cuts at: 0 63 981
Size: 63 918
AlwNI CAG_nnn'CTG
Cuts at: 0 228 426 726 861 959 981
Size: 228 198 300 135 98 22
Fragments arranged by size:
300 228 198 135 98 22
AvaI C'yCGr_G
Cuts at: 0 760 981
Size: 760 221
BanI G'GyrC_C
Cuts at: 0 52 107 239 981
Size: 52 55 132 742
Fragments arranged by size:
742 132 55 52
BanII G_rGCy'C
Cuts at: 0 96 629 937 981
Size: 96 533 308 44
Fragments arranged by size:
533 308 96 44
BbeI G_GCGC'C
Cuts at: 0 111 243 981
Size: 111 132 738
Fragments arranged by size:
738 132 111
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 591 625 981
Size: 591 34 356
Fragments arranged by size:
591 356 34
BglI GCCn_nnn'nGGC
Cuts at: 0 200 237 871 981
Size: 200 37 634 110
Fragments arranged by size:
634 200 110 37
BmrI ACTGGGnnnn_n'
Cuts at: 0 30 51 981
Size: 30 21 930
Fragments arranged by size:
930 30 21
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 453 981
Size: 453 528
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 11 174 448 723 981
Size: 11 163 274 275 258
Fragments arranged by size:
275 274 258 163 11
Bpu10I CC'TnA_GC
Cuts at: 0 101 981
Size: 101 880
BsaI GGTCTCn'nnnn_
Cuts at: 0 134 981
Size: 134 847
BsaHI Gr'CG_yC
Cuts at: 0 108 240 445 581 981
Size: 108 132 205 136 400
Fragments arranged by size:
400 205 136 132 108
BsaWI w'CCGG_w
Cuts at: 0 63 981
Size: 63 918
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 511 541 634 664 981
Size: 511 30 93 30 317
Fragments arranged by size:
511 317 93 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 106 238 637 981
Size: 106 132 399 344
Fragments arranged by size:
399 344 132 106
BseYI C'CCAG_C
Cuts at: 0 483 815 981
Size: 483 332 166
Fragments arranged by size:
483 332 166
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 61 621 981
Size: 61 560 360
Fragments arranged by size:
560 360 61
BsmBI CGTCTCn'nnnn_
Cuts at: 0 588 981
Size: 588 393
Bsp1286I G_dGCh'C
Cuts at: 0 96 629 937 981
Size: 96 533 308 44
Fragments arranged by size:
533 308 96 44
BspHI T'CATG_A
Cuts at: 0 744 981
Size: 744 237
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 720 981
Size: 720 261
BsrBI CCG'CTC
Cuts at: 0 114 175 981
Size: 114 61 806
Fragments arranged by size:
806 114 61
BsrFI r'CCGG_y
Cuts at: 0 63 547 981
Size: 63 484 434
Fragments arranged by size:
484 434 63
BstAPI GCAn_nnn'nTGC
Cuts at: 0 75 228 614 726 981
Size: 75 153 386 112 255
Fragments arranged by size:
386 255 153 112 75
BstEII G'GTnAC_C
Cuts at: 0 177 981
Size: 177 804
BstYI r'GATC_y
Cuts at: 0 797 981
Size: 797 184
BtgI C'CryG_G
Cuts at: 0 195 680 929 981
Size: 195 485 249 52
Fragments arranged by size:
485 249 195 52
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 594 981
Size: 594 387
EaeI y'GGCC_r
Cuts at: 0 192 201 362 545 784 803 863 926
Size: 192 9 161 183 239 19 60 63
Cuts at: 926 981
Size: 55
Fragments arranged by size:
239 192 183 161 63 60 55 19
9
EarI CTCTTCn'nnn_
Cuts at: 0 124 981
Size: 124 857
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 861 981
Size: 861 120
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 11 174 289 448 481 723 981
Size: 11 163 115 159 33 242 258
Fragments arranged by size:
258 242 163 159 115 33 11
EcoNI CCTnn'n_nnAGG
Cuts at: 0 253 292 981
Size: 253 39 689
Fragments arranged by size:
689 253 39
EcoO109I rG'GnC_Cy
Cuts at: 0 264 285 723 981
Size: 264 21 438 258
Fragments arranged by size:
438 264 258 21
HaeII r_GCGC'y
Cuts at: 0 111 243 308 694 887 981
Size: 111 132 65 386 193 94
Fragments arranged by size:
386 193 132 111 94 65
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 511 543 547 554 579 586 634 666
Size: 511 32 4 7 25 7 48 32
Cuts at: 666 981
Size: 315
Fragments arranged by size:
511 315 48 32 32 25 7 7
4
KasI G'GCGC_C
Cuts at: 0 107 239 981
Size: 107 132 742
Fragments arranged by size:
742 132 107
MscI TGG'CCA
Cuts at: 0 194 805 981
Size: 194 611 176
Fragments arranged by size:
611 194 176
MslI CAynn'nnrTG
Cuts at: 0 493 832 981
Size: 493 339 149
Fragments arranged by size:
493 339 149
MspA1I CmG'CkG
Cuts at: 0 682 889 931 948 981
Size: 682 207 42 17 33
Fragments arranged by size:
682 207 42 33 17
NaeI GCC'GGC
Cuts at: 0 549 981
Size: 549 432
NarI GG'CG_CC
Cuts at: 0 108 240 981
Size: 108 132 741
Fragments arranged by size:
741 132 108
NgoMIV G'CCGG_C
Cuts at: 0 547 981
Size: 547 434
NspI r_CATG'y
Cuts at: 0 73 492 981
Size: 73 419 489
Fragments arranged by size:
489 419 73
PasI CC'CwG_GG
Cuts at: 0 234 981
Size: 234 747
PflMI CCAn_nnn'nTGG
Cuts at: 0 900 981
Size: 900 81
PpuMI rG'GwC_Cy
Cuts at: 0 264 285 981
Size: 264 21 696
Fragments arranged by size:
696 264 21
PstI C_TGCA'G
Cuts at: 0 164 224 526 731 981
Size: 164 60 302 205 250
Fragments arranged by size:
302 250 205 164 60
SacII CC_GC'GG
Cuts at: 0 683 932 981
Size: 683 249 49
Fragments arranged by size:
683 249 49
SbfI CC_TGCA'GG
Cuts at: 0 731 981
Size: 731 250
SfcI C'TryA_G
Cuts at: 0 160 220 522 727 981
Size: 160 60 302 205 254
Fragments arranged by size:
302 254 205 160 60
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 200 871 981
Size: 200 671 110
Fragments arranged by size:
671 200 110
SfoI GGC'GCC
Cuts at: 0 109 241 981
Size: 109 132 740
Fragments arranged by size:
740 132 109
SgrAI Cr'CCGG_yG
Cuts at: 0 63 981
Size: 63 918
SmaI CCC'GGG
Cuts at: 0 762 981
Size: 762 219
SphI G_CATG'C
Cuts at: 0 73 492 981
Size: 73 419 489
Fragments arranged by size:
489 419 73
StyI C'CwwG_G
Cuts at: 0 938 981
Size: 938 43
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 70 981
Size: 70 911
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 307 339 981
Size: 307 32 642
Fragments arranged by size:
642 307 32
XmaI C'CCGG_G
Cuts at: 0 760 981
Size: 760 221
ZraI GAC'GTC
Cuts at: 0 446 582 981
Size: 446 136 399
Fragments arranged by size:
446 399 136
Enzymes that do cut:
AarI AatII AcuI AfeI AgeI AlwNI AvaI BanI
BanII BbeI BcgI BglI BmrI BplI BpmI Bpu10I
BsaI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsmBI
Bsp1286I BspHI BspMI BsrBI BsrFI BstAPI BstEII BstYI
BtgI BtgZI EaeI EarI EciI Eco57MI EcoNI EcoO109I
HaeII Hin4I KasI MscI MslI MspA1I NaeI NarI
NgoMIV NspI PasI PflMI PpuMI PstI SacII SbfI
SfcI SfiI SfoI SgrAI SmaI SphI StyI TaqII
TstI XmaI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AflII AflIII AhdI AleI AlfI
AloI ApaI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BbsI BbvCI BciVI BclI BfrBI BglII
BlpI Bme1580I BmgBI BmtI BpuEI BsaAI BsaBI BsiEI
BsiHKAI BsiWI BsmI BspEI BsrDI BsrGI BssHII BssSI
BstBI BstXI BstZ17I Bsu36I BtsI ClaI CspCI DraI
DraIII DrdI EagI EcoICRI EcoRI EcoRV FalI FseI
FspI FspAI HincII HindIII HpaI KpnI MfeI MluI
MmeI NcoI NdeI NheI NotI NruI NsiI PacI
PciI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI
PspXI PsrI PvuI PvuII RsrII SacI SalI SanDI
SapI ScaI SexAI SgfI SmlI SnaBI SpeI SrfI
SspI StuI SwaI TatI Tth111I XbaI XcmI XhoI
XmnI