Restriction map for
pF1KA1662
(Linear) (Six-base) MAPSORT of: KIAA1662.seq Check: 711 from: 1 to: 7095
KIAA1662.orf
With 182 enzymes: SgfI *
July 15, 2010 18:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 180 3189 4050 6360 6597 7043 7095
Size: 180 3009 861 2310 237 446 52
Fragments arranged by size:
3009 2310 861 446 237 180 52
AatII G_ACGT'C
Cuts at: 0 6971 7095
Size: 6971 124
Acc65I G'GTAC_C
Cuts at: 0 3344 7095
Size: 3344 3751
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 113 3301 5651 5767 6613 7045 7095
Size: 113 3188 2350 116 846 432 50
Fragments arranged by size:
3188 2350 846 432 116 113 50
AfeI AGC'GCT
Cuts at: 0 5944 6943 7095
Size: 5944 999 152
Fragments arranged by size:
5944 999 152
AflIII A'CryG_T
Cuts at: 0 814 7095
Size: 814 6281
AgeI A'CCGG_T
Cuts at: 0 707 7095
Size: 707 6388
AhdI GACnn_n'nnGTC
Cuts at: 0 5657 7095
Size: 5657 1438
AleI CACnn'nnGTG
Cuts at: 0 3333 3487 4606 7095
Size: 3333 154 1119 2489
Fragments arranged by size:
3333 2489 1119 154
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3080 3524 3680 7095
Size: 3080 444 156 3415
Fragments arranged by size:
3415 3080 444 156
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1239 1271 1386 1418 1533 1565 1680 1712
Size: 1239 32 115 32 115 32 115 32
Cuts at: 1712 1827 1859 7095
Size: 115 32 5236
Fragments arranged by size:
5236 1239 115 115 115 115 32 32
32 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 136 4269 6096 6417 6717 6738 7095
Size: 136 4133 1827 321 300 21 357
Fragments arranged by size:
4133 1827 357 321 300 136 21
ApaI G_GGCC'C
Cuts at: 0 248 259 3866 4315 4366 4725 5056 5086
Size: 248 11 3607 449 51 359 331 30
Cuts at: 5086 5743 5877 6179 6231 6550 7095
Size: 657 134 302 52 319 545
Fragments arranged by size:
3607 657 545 449 359 331 319 302
248 134 52 51 30 11
ApaLI G'TGCA_C
Cuts at: 0 6272 7095
Size: 6272 823
ApoI r'AATT_y
Cuts at: 0 1200 2679 7095
Size: 1200 1479 4416
Fragments arranged by size:
4416 1479 1200
AvaI C'yCGr_G
Cuts at: 0 522 533 2921 3071 3212 3359 3671 3755
Size: 522 11 2388 150 141 147 312 84
Cuts at: 3755 3772 4663 5257 6306 6951 7095
Size: 17 891 594 1049 645 144
Fragments arranged by size:
2388 1049 891 645 594 522 312 150
147 144 141 84 17 11
AvrII C'CTAG_G
Cuts at: 0 958 7095
Size: 958 6137
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 2968 3001 6865 6898 7095
Size: 2968 33 3864 33 197
Fragments arranged by size:
3864 2968 197 33 33
BamHI G'GATC_C
Cuts at: 0 3567 7095
Size: 3567 3528
BanI G'GyrC_C
Cuts at: 0 9 141 704 3056 3344 3500 3656 3720
Size: 9 132 563 2352 288 156 156 64
Cuts at: 3720 4701 5997 6031 6129 6392 7095
Size: 981 1296 34 98 263 703
Fragments arranged by size:
2352 1296 981 703 563 288 263 156
156 132 98 64 34 9
BanII G_rGCy'C
Cuts at: 0 119 127 248 259 278 509 570 3006
Size: 119 8 121 11 19 231 61 2436
Cuts at: 3006 3026 3131 3314 3754 3763 3866 4315 4366
Size: 20 105 183 440 9 103 449 51
Cuts at: 4366 4394 4505 4543 4725 4763 4951 5056 5086
Size: 28 111 38 182 38 188 105 30
Cuts at: 5086 5221 5743 5773 5843 5877 6179 6231 6550
Size: 135 522 30 70 34 302 52 319
Cuts at: 6550 6920 7095
Size: 370 175
Fragments arranged by size:
2436 522 449 440 370 319 302 231
188 183 182 175 135 121 119 111
105 105 103 70 61 52 51 38
38 34 30 30 28 20 19 11
9 8
BbsI GAAGACnn'nnnn_
Cuts at: 0 4038 7095
Size: 4038 3057
BbvCI CC'TCA_GC
Cuts at: 0 137 267 5480 6446 6468 6668 7095
Size: 137 130 5213 966 22 200 427
Fragments arranged by size:
5213 966 427 200 137 130 22
BglI GCCn_nnn'nGGC
Cuts at: 0 3001 3214 3973 4084 4253 4370 5792 6554
Size: 3001 213 759 111 169 117 1422 762
Cuts at: 6554 7095
Size: 541
Fragments arranged by size:
3001 1422 762 759 541 213 169 117
111
BlpI GC'TnA_GC
Cuts at: 0 3762 6508 6520 6982 7095
Size: 3762 2746 12 462 113
Fragments arranged by size:
3762 2746 462 113 12
Bme1580I G_kGCm'C
Cuts at: 0 146 248 259 1221 1368 1446 1515 1662
Size: 146 102 11 962 147 78 69 147
Cuts at: 1662 1740 1809 1887 1959 2031 2103 2175 2247
Size: 78 69 78 72 72 72 72 72
Cuts at: 2247 2388 3059 3472 3503 3659 3866 4315 4366
Size: 141 671 413 31 156 207 449 51
Cuts at: 4366 4438 4704 4725 5056 5086 5743 5877 6036
Size: 72 266 21 331 30 657 134 159
Cuts at: 6036 6134 6179 6231 6276 6550 6654 7095
Size: 98 45 52 45 274 104 441
Fragments arranged by size:
962 671 657 449 441 413 331 274
266 207 159 156 147 147 146 141
134 104 102 98 78 78 78 72
72 72 72 72 72 69 69 52
51 45 45 31 30 21 11
BmgBI CAC'GTC
Cuts at: 0 767 7095
Size: 767 6328
BmrI ACTGGGnnnn_n'
Cuts at: 0 468 672 2877 2898 3622 3695 4563 4752
Size: 468 204 2205 21 724 73 868 189
Cuts at: 4752 4906 4980 7095
Size: 154 74 2115
Fragments arranged by size:
2205 2115 868 724 468 204 189 154
74 73 21
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 548 2545 5400 6642 7095
Size: 548 1997 2855 1242 453
Fragments arranged by size:
2855 1997 1242 548 453
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 364 390 718 852 882 912 1227 1374
Size: 364 26 328 134 30 30 315 147
Cuts at: 1374 1521 1668 1815 4150 4258 4359 4695 5062
Size: 147 147 147 2335 108 101 336 367
Cuts at: 5062 5393 5944 6118 6223 6256 6523 6661 6862
Size: 331 551 174 105 33 267 138 201
Cuts at: 6862 6904 7042 7095
Size: 42 138 53
Fragments arranged by size:
2335 551 367 364 336 331 328 315
267 201 174 147 147 147 147 138
138 134 108 105 101 53 42 33
30 30 26
Bpu10I CC'TnA_GC
Cuts at: 0 118 137 267 800 3020 3545 4865 5480
Size: 118 19 130 533 2220 525 1320 615
Cuts at: 5480 6446 6468 6668 7095
Size: 966 22 200 427
Fragments arranged by size:
2220 1320 966 615 533 525 427 200
130 118 22 19
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 346 5209 7095
Size: 346 4863 1886
Fragments arranged by size:
4863 1886 346
BsaI GGTCTCn'nnnn_
Cuts at: 0 2628 3772 4137 4224 7095
Size: 2628 1144 365 87 2871
Fragments arranged by size:
2871 2628 1144 365 87
BsaAI yAC'GTr
Cuts at: 0 817 5835 7095
Size: 817 5018 1260
Fragments arranged by size:
5018 1260 817
BsaBI GATnn'nnATC
Cuts at: 0 5356 7095
Size: 5356 1739
BsaHI Gr'CG_yC
Cuts at: 0 3217 6246 6968 7095
Size: 3217 3029 722 127
Fragments arranged by size:
3217 3029 722 127
BsaWI w'CCGG_w
Cuts at: 0 707 6560 7095
Size: 707 5853 535
Fragments arranged by size:
5853 707 535
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 388 418 1098 1128 1239 1269 1386 1416
Size: 388 30 680 30 111 30 117 30
Cuts at: 1416 1533 1563 1680 1710 1827 1857 2328 2358
Size: 117 30 117 30 117 30 471 30
Cuts at: 2358 2835 2865 4700 4730 5455 5485 6418 6448
Size: 477 30 1835 30 725 30 933 30
Cuts at: 6448 6521 6551 6886 6916 7054 7084 7095
Size: 73 30 335 30 138 30 11
Fragments arranged by size:
1835 933 725 680 477 471 388 335
138 117 117 117 117 111 73 30
30 30 30 30 30 30 30 30
30 30 30 30 30 30 11
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 19 103 496 499 540 568 614 825
Size: 19 84 393 3 41 28 46 211
Cuts at: 825 1161 1242 1311 1389 1458 1536 1605 1683
Size: 336 81 69 78 69 78 69 78
Cuts at: 1683 1752 1830 1899 1971 2043 2115 2187 2259
Size: 69 78 69 72 72 72 72 72
Cuts at: 2259 2331 2400 2589 3357 3669 3817 4498 5131
Size: 72 69 189 768 312 148 681 633
Cuts at: 5131 5140 5497 5932 5965 6085 6254 6394 6463
Size: 9 357 435 33 120 169 140 69
Cuts at: 6463 6685 6772 7095
Size: 222 87 323
Fragments arranged by size:
768 681 633 435 393 357 336 323
312 222 211 189 169 148 140 120
87 84 81 78 78 78 78 72
72 72 72 72 72 69 69 69
69 69 69 69 46 41 33 28
19 9 3
BseYI C'CCAG_C
Cuts at: 0 493 497 1076 1118 1220 1298 1367 1445
Size: 493 4 579 42 102 78 69 78
Cuts at: 1445 1514 1592 1661 1739 1808 1886 2059 2246
Size: 69 78 69 78 69 78 173 187
Cuts at: 2246 2387 2854 2867 3029 4003 4051 4393 4460
Size: 141 467 13 162 974 48 342 67
Cuts at: 4460 4613 4732 4791 4836 4895 4963 5086 5212
Size: 153 119 59 45 59 68 123 126
Cuts at: 5212 5883 5939 6230 7095
Size: 671 56 291 865
Fragments arranged by size:
974 865 671 579 493 467 342 291
187 173 162 153 141 126 123 119
102 78 78 78 78 78 69 69
69 69 68 67 59 59 56 48
45 42 13 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1322 1616 2598 3311 3445 3897 3932 4959
Size: 1322 294 982 713 134 452 35 1027
Cuts at: 4959 5508 5569 5701 6919 7095
Size: 549 61 132 1218 176
Fragments arranged by size:
1322 1218 1027 982 713 549 452 294
176 134 132 61 35
BsiEI CG_ry'CG
Cuts at: 0 5024 7095
Size: 5024 2071
BsiHKAI G_wGCw'C
Cuts at: 0 119 509 2962 4505 4763 5074 5843 6276
Size: 119 390 2453 1543 258 311 769 433
Cuts at: 6276 6683 6920 7034 7095
Size: 407 237 114 61
Fragments arranged by size:
2453 1543 769 433 407 390 311 258
237 119 114 61
BsiWI C'GTAC_G
Cuts at: 0 5655 7095
Size: 5655 1440
BsmBI CGTCTCn'nnnn_
Cuts at: 0 578 773 6253 6445 7095
Size: 578 195 5480 192 650
Fragments arranged by size:
5480 650 578 195 192
Bsp1286I G_dGCh'C
Cuts at: 0 119 127 146 248 259 278 509 570
Size: 119 8 19 102 11 19 231 61
Cuts at: 570 1221 1368 1446 1515 1662 1740 1809 1887
Size: 651 147 78 69 147 78 69 78
Cuts at: 1887 1959 2031 2103 2175 2247 2388 2962 3006
Size: 72 72 72 72 72 141 574 44
Cuts at: 3006 3026 3059 3131 3314 3472 3503 3659 3754
Size: 20 33 72 183 158 31 156 95
Cuts at: 3754 3763 3866 4315 4366 4394 4438 4505 4543
Size: 9 103 449 51 28 44 67 38
Cuts at: 4543 4704 4725 4763 4951 5056 5074 5086 5221
Size: 161 21 38 188 105 18 12 135
Cuts at: 5221 5743 5773 5843 5877 6036 6134 6179 6231
Size: 522 30 70 34 159 98 45 52
Cuts at: 6231 6276 6550 6654 6683 6920 7034 7095
Size: 45 274 104 29 237 114 61
Fragments arranged by size:
651 574 522 449 274 237 231 188
183 161 159 158 156 147 147 141
135 119 114 105 104 103 102 98
95 78 78 78 72 72 72 72
72 72 70 69 69 67 61 61
52 51 45 45 44 44 38 38
34 33 31 30 29 28 21 20
19 19 18 12 11 9 8
BspEI T'CCGG_A
Cuts at: 0 6560 7095
Size: 6560 535
BspHI T'CATG_A
Cuts at: 0 4497 7095
Size: 4497 2598
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 164 180 3189 3833 4050 5522 6360 6597
Size: 164 16 3009 644 217 1472 838 237
Cuts at: 6597 7043 7095
Size: 446 52
Fragments arranged by size:
3009 1472 838 644 446 237 217 164
52 16
BsrBI CCG'CTC
Cuts at: 0 702 2654 3798 5223 5928 5955 6402 6828
Size: 702 1952 1144 1425 705 27 447 426
Cuts at: 6828 7008 7095
Size: 180 87
Fragments arranged by size:
1952 1425 1144 705 702 447 426 180
87 27
BsrDI GCAATG_nn'
Cuts at: 0 6782 6810 7095
Size: 6782 28 285
Fragments arranged by size:
6782 285 28
BsrFI r'CCGG_y
Cuts at: 0 631 707 5264 6059 7095
Size: 631 76 4557 795 1036
Fragments arranged by size:
4557 1036 795 631 76
BsrGI T'GTAC_A
Cuts at: 0 2542 7095
Size: 2542 4553
BssSI C'ACGA_G
Cuts at: 0 1271 1340 1418 1487 1565 1634 1712 1781
Size: 1271 69 78 69 78 69 78 69
Cuts at: 1781 1859 2360 3160 3307 3463 4536 6193 6397
Size: 78 501 800 147 156 1073 1657 204
Cuts at: 6397 7095
Size: 698
Fragments arranged by size:
1657 1271 1073 800 698 501 204 156
147 78 78 78 78 69 69 69
69
BstAPI GCAn_nnn'nTGC
Cuts at: 0 6417 6687 6809 7095
Size: 6417 270 122 286
Fragments arranged by size:
6417 286 270 122
BstEII G'GTnAC_C
Cuts at: 0 4062 5238 7095
Size: 4062 1176 1857
Fragments arranged by size:
4062 1857 1176
BstXI CCAn_nnnn'nTGG
Cuts at: 0 3843 4478 4608 4895 6292 7095
Size: 3843 635 130 287 1397 803
Fragments arranged by size:
3843 1397 803 635 287 130
BstYI r'GATC_y
Cuts at: 0 3567 4113 4680 5570 6280 7095
Size: 3567 546 567 890 710 815
Fragments arranged by size:
3567 890 815 710 567 546
Bsu36I CC'TnA_GG
Cuts at: 0 86 179 235 4148 5123 5308 5613 7095
Size: 86 93 56 3913 975 185 305 1482
Fragments arranged by size:
3913 1482 975 305 185 93 86 56
BtgI C'CryG_G
Cuts at: 0 96 3449 6159 6461 7046 7095
Size: 96 3353 2710 302 585 49
Fragments arranged by size:
3353 2710 585 302 96 49
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2793 7095
Size: 2793 4302
BtsI GCAGTG_nn'
Cuts at: 0 518 794 3880 4601 4933 5072 5422 5474
Size: 518 276 3086 721 332 139 350 52
Cuts at: 5474 6137 6907 7095
Size: 663 770 188
Fragments arranged by size:
3086 770 721 663 518 350 332 276
188 139 52
DraIII CAC_nnn'GTG
Cuts at: 0 748 1144 3703 4988 7095
Size: 748 396 2559 1285 2107
Fragments arranged by size:
2559 2107 1285 748 396
EaeI y'GGCC_r
Cuts at: 0 2734 2818 3818 3906 4245 4814 4990 5603
Size: 2734 84 1000 88 339 569 176 613
Cuts at: 5603 5906 6189 6294 6744 7049 7095
Size: 303 283 105 450 305 46
Fragments arranged by size:
2734 1000 613 569 450 339 305 303
283 176 105 88 84 46
EarI CTCTTCn'nnn_
Cuts at: 0 278 1074 2769 2937 3084 3562 3934 4135
Size: 278 796 1695 168 147 478 372 201
Cuts at: 4135 6227 6497 7095
Size: 2092 270 598
Fragments arranged by size:
2092 1695 796 598 478 372 278 270
201 168 147
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 408 4116 5265 5640 5821 7095
Size: 408 3708 1149 375 181 1274
Fragments arranged by size:
3708 1274 1149 408 375 181
EcoICRI GAG'CTC
Cuts at: 0 117 507 4503 4761 5841 6918 7095
Size: 117 390 3996 258 1080 1077 177
Fragments arranged by size:
3996 1080 1077 390 258 177 117
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 113 364 390 718 852 882 912 1227
Size: 113 251 26 328 134 30 30 315
Cuts at: 1227 1374 1521 1668 1815 3301 4150 4258 4359
Size: 147 147 147 147 1486 849 108 101
Cuts at: 4359 4695 5062 5393 5651 5767 5944 6118 6223
Size: 336 367 331 258 116 177 174 105
Cuts at: 6223 6256 6523 6613 6661 6862 6904 7042 7045
Size: 33 267 90 48 201 42 138 3
Cuts at: 7045 7095
Size: 50
Fragments arranged by size:
1486 849 367 336 331 328 315 267
258 251 201 177 174 147 147 147
147 138 134 116 113 108 105 101
90 50 48 42 33 30 30 26
3
EcoNI CCTnn'n_nnAGG
Cuts at: 0 596 6043 6421 6445 6754 6889 7095
Size: 596 5447 378 24 309 135 206
Fragments arranged by size:
5447 596 378 309 206 135 24
EcoO109I rG'GnC_Cy
Cuts at: 0 183 244 255 289 348 384 489 587
Size: 183 61 11 34 59 36 105 98
Cuts at: 587 872 902 932 962 992 2336 3384 3411
Size: 285 30 30 30 30 1344 1048 27
Cuts at: 3411 3862 3863 3965 4172 4202 4304 4311 4312
Size: 451 1 102 207 30 102 7 1
Cuts at: 4312 4363 4561 4749 4788 4840 5053 5082 5083
Size: 51 198 188 39 52 213 29 1
Cuts at: 5083 5304 5739 5740 5931 5987 6024 6175 6176
Size: 221 435 1 191 56 37 151 1
Cuts at: 6176 6546 7095
Size: 370 549
Fragments arranged by size:
1344 1048 549 451 435 370 285 221
213 207 198 191 188 183 151 105
102 102 98 61 59 56 52 51
39 37 36 34 30 30 30 30
30 29 27 11 7 1 1 1
1 1
EcoRI G'AATT_C
Cuts at: 0 1200 2679 7095
Size: 1200 1479 4416
Fragments arranged by size:
4416 1479 1200
FspI TGC'GCA
Cuts at: 0 7078 7095
Size: 7078 17
HaeII r_GCGC'y
Cuts at: 0 197 3769 3927 5946 6171 6417 6945 7095
Size: 197 3572 158 2019 225 246 528 150
Fragments arranged by size:
3572 2019 528 246 225 197 158 150
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 376 388 408 420 2328 2360 2736 2768
Size: 376 12 20 12 1908 32 376 32
Cuts at: 2768 2835 2867 3499 3531 3976 4008 4700 4732
Size: 67 32 632 32 445 32 692 32
Cuts at: 4732 4858 4890 5013 5045 5625 5643 5657 5675
Size: 126 32 123 32 580 18 14 18
Cuts at: 5675 6340 6372 7054 7086 7095
Size: 665 32 682 32 9
Fragments arranged by size:
1908 692 682 665 632 580 445 376
376 126 123 67 32 32 32 32
32 32 32 32 32 32 20 18
18 14 12 12 9
HincII GTy'rAC
Cuts at: 0 713 7095
Size: 713 6382
KpnI G_GTAC'C
Cuts at: 0 3348 7095
Size: 3348 3747
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2025 2097 2313 5133 5686 7095
Size: 2025 72 216 2820 553 1409
Fragments arranged by size:
2820 2025 1409 553 216 72
MscI TGG'CCA
Cuts at: 0 4247 4816 4992 5908 6296 7095
Size: 4247 569 176 916 388 799
Fragments arranged by size:
4247 916 799 569 388 176
MslI CAynn'nnrTG
Cuts at: 0 2607 3062 3203 3333 3487 3506 3662 4606
Size: 2607 455 141 130 154 19 156 944
Cuts at: 4606 4805 7095
Size: 199 2290
Fragments arranged by size:
2607 2290 944 455 199 156 154 141
130 19
MspA1I CmG'CkG
Cuts at: 0 65 298 374 497 1122 1224 1302 1371
Size: 65 233 76 123 625 102 78 69
Cuts at: 1371 1449 1518 1596 1665 1743 1812 1890 2250
Size: 78 69 78 69 78 69 78 360
Cuts at: 2250 2391 2550 2992 3884 3961 4179 4795 4959
Size: 141 159 442 892 77 218 616 164
Cuts at: 4959 5763 5850 5871 6161 6234 6390 6695 6771
Size: 804 87 21 290 73 156 305 76
Cuts at: 6771 7023 7095
Size: 252 72
Fragments arranged by size:
892 804 625 616 442 360 305 290
252 233 218 164 159 156 141 123
102 87 78 78 78 78 78 77
76 76 73 72 69 69 69 69
65 21
NcoI C'CATG_G
Cuts at: 0 96 3449 7046 7095
Size: 96 3353 3597 49
Fragments arranged by size:
3597 3353 96 49
NspI r_CATG'y
Cuts at: 0 6332 7095
Size: 6332 763
PasI CC'CwG_GG
Cuts at: 0 989 2714 2798 3419 4308 4366 5058 7095
Size: 989 1725 84 621 889 58 692 2037
Fragments arranged by size:
2037 1725 989 889 692 621 84 58
PflMI CCAn_nnn'nTGG
Cuts at: 0 219 748 2663 4918 4988 7095
Size: 219 529 1915 2255 70 2107
Fragments arranged by size:
2255 2107 1915 529 219 70
PfoI T'CCnGG_A
Cuts at: 0 3407 3563 6908 7058 7095
Size: 3407 156 3345 150 37
Fragments arranged by size:
3407 3345 156 150 37
PmlI CAC'GTG
Cuts at: 0 817 7095
Size: 817 6278
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1239 1271 1386 1418 1533 1565 1680 1712
Size: 1239 32 115 32 115 32 115 32
Cuts at: 1712 1827 1859 5089 5121 7095
Size: 115 32 3230 32 1974
Fragments arranged by size:
3230 1974 1239 115 115 115 115 32
32 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 183 289 384 587 2336 3411 4172 4202
Size: 183 106 95 203 1749 1075 761 30
Cuts at: 4202 4304 4788 4840 5304 5931 5987 7095
Size: 102 484 52 464 627 56 1108
Fragments arranged by size:
1749 1108 1075 761 627 484 464 203
183 106 102 95 56 52 30
PspOMI G'GGCC_C
Cuts at: 0 244 255 3862 4311 4362 4721 5052 5082
Size: 244 11 3607 449 51 359 331 30
Cuts at: 5082 5739 5873 6175 6227 6546 7095
Size: 657 134 302 52 319 549
Fragments arranged by size:
3607 657 549 449 359 331 319 302
244 134 52 51 30 11
PspXI vC'TCGA_Gb
Cuts at: 0 3071 7095
Size: 3071 4024
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1056 1088 2298 2330 7095
Size: 1056 32 1210 32 4765
Fragments arranged by size:
4765 1210 1056 32 32
PstI C_TGCA'G
Cuts at: 0 612 4769 4940 5105 6389 6413 6422 6608
Size: 612 4157 171 165 1284 24 9 186
Cuts at: 6608 7095
Size: 487
Fragments arranged by size:
4157 1284 612 487 186 171 165 24
9
PvuII CAG'CTG
Cuts at: 0 298 374 497 4795 4959 6234 6771 7023
Size: 298 76 123 4298 164 1275 537 252
Cuts at: 7023 7095
Size: 72
Fragments arranged by size:
4298 1275 537 298 252 164 123 76
72
SacI G_AGCT'C
Cuts at: 0 119 509 4505 4763 5843 6920 7095
Size: 119 390 3996 258 1080 1077 175
Fragments arranged by size:
3996 1080 1077 390 258 175 119
SacII CC_GC'GG
Cuts at: 0 6162 7095
Size: 6162 933
SanDI GG'GwC_CC
Cuts at: 0 289 4172 4304 4840 7095
Size: 289 3883 132 536 2255
Fragments arranged by size:
3883 2255 536 289 132
SapI GCTCTTCn'nnn_
Cuts at: 0 6227 6497 7095
Size: 6227 270 598
Fragments arranged by size:
6227 598 270
SbfI CC_TGCA'GG
Cuts at: 0 4769 7095
Size: 4769 2326
ScaI AGT'ACT
Cuts at: 0 6625 7095
Size: 6625 470
SexAI A'CCwGG_T
Cuts at: 0 583 7095
Size: 583 6512
SfcI C'TryA_G
Cuts at: 0 608 4765 4936 5101 5724 6385 6409 6418
Size: 608 4157 171 165 623 661 24 9
Cuts at: 6418 6604 7095
Size: 186 491
Fragments arranged by size:
4157 661 623 608 491 186 171 165
24 9
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 3973 4253 6554 7095
Size: 3973 280 2301 541
Fragments arranged by size:
3973 2301 541 280
SgrAI Cr'CCGG_yG
Cuts at: 0 631 7095
Size: 631 6464
SmaI CCC'GGG
Cuts at: 0 2923 3214 3361 3757 3774 5259 6308 7095
Size: 2923 291 147 396 17 1485 1049 787
Fragments arranged by size:
2923 1485 1049 787 396 291 147 17
SmlI C'TyrA_G
Cuts at: 0 325 3071 5224 7095
Size: 325 2746 2153 1871
Fragments arranged by size:
2746 2153 1871 325
SpeI A'CTAG_T
Cuts at: 0 3580 7095
Size: 3580 3515
SspI AAT'ATT
Cuts at: 0 5461 7095
Size: 5461 1634
StuI AGG'CCT
Cuts at: 0 233 531 2838 4358 4477 5039 5824 6493
Size: 233 298 2307 1520 119 562 785 669
Cuts at: 6493 7095
Size: 602
Fragments arranged by size:
2307 1520 785 669 602 562 298 233
119
StyI C'CwwG_G
Cuts at: 0 96 958 2826 3449 4008 5579 5825 6252
Size: 96 862 1868 623 559 1571 246 427
Cuts at: 6252 7046 7095
Size: 794 49
Fragments arranged by size:
1868 1571 862 794 623 559 427 246
96 49
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 868 898 928 958 988 1060 1942 2158
Size: 868 30 30 30 30 72 882 216
Cuts at: 2158 2443 2632 2917 3253 3710 3714 3849 4614
Size: 285 189 285 336 457 4 135 765
Cuts at: 4614 4999 5746 6019 7095
Size: 385 747 273 1076
Fragments arranged by size:
1076 882 868 765 747 457 385 336
285 285 273 216 189 135 72 30
30 30 30 4
TatI w'GTAC_w
Cuts at: 0 1111 2542 2906 3806 4448 6623 7095
Size: 1111 1431 364 900 642 2175 472
Fragments arranged by size:
2175 1431 1111 900 642 472 364
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 10 42 806 838 1128 1130 1160 1162
Size: 10 32 764 32 290 2 30 2
Cuts at: 1162 6418 6450 6521 6553 6886 6918 7095
Size: 5256 32 71 32 333 32 177
Fragments arranged by size:
5256 764 333 290 177 71 32 32
32 32 32 30 10 2 2
Tth111I GACn'n_nGTC
Cuts at: 0 6999 7095
Size: 6999 96
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 591 2890 3571 3789 6739 7095
Size: 591 2299 681 218 2950 356
Fragments arranged by size:
2950 2299 681 591 356 218
XhoI C'TCGA_G
Cuts at: 0 3071 7095
Size: 3071 4024
XmaI C'CCGG_G
Cuts at: 0 2921 3212 3359 3755 3772 5257 6306 7095
Size: 2921 291 147 396 17 1485 1049 789
Fragments arranged by size:
2921 1485 1049 789 396 291 147 17
XmnI GAAnn'nnTTC
Cuts at: 0 1068 2763 7095
Size: 1068 1695 4332
Fragments arranged by size:
4332 1695 1068
ZraI GAC'GTC
Cuts at: 0 6969 7095
Size: 6969 126
Enzymes that do cut:
AarI AatII Acc65I AcuI AfeI AflIII AgeI AhdI
AleI AlfI AloI AlwNI ApaI ApaLI ApoI AvaI
AvrII BaeI BamHI BanI BanII BbsI BbvCI BglI
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsiWI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI DraIII EaeI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII KpnI MmeI MscI MslI MspA1I
NcoI NspI PasI PflMI PfoI PmlI PpiI PpuMI
PspOMI PspXI PsrI PstI PvuII SacI SacII SanDI
SapI SbfI ScaI SexAI SfcI SfiI SgrAI SmaI
SmlI SpeI SspI StuI StyI TaqII TatI TstI
Tth111I XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AccI AclI AflII AscI AseI AsiSI BbeI BcgI
BciVI BclI BfrBI BglII BmtI BsmI BssHII BstBI
BstZ17I ClaI CspCI DraI DrdI EagI EcoRV FalI
FseI FspAI HindIII HpaI KasI MfeI MluI NaeI
NarI NdeI NgoMIV NheI NotI NruI NsiI PacI
PciI PmeI PshAI PsiI PvuI RsrII SalI SfoI
SgfI SnaBI SphI SrfI SwaI XbaI