Restriction map for
pF1KSDA1665
(Linear) (Six-base) MAPSORT of: KIAA1665.seq Check: 140 from: 1 to: 612
KIAA1665.orf
With 182 enzymes: SgfI *
October 24, 2006 15:17 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 363 612
Size: 363 249
AflIII A'CryG_T
Cuts at: 0 476 612
Size: 476 136
AleI CACnn'nnGTG
Cuts at: 0 556 612
Size: 556 56
ApaI G_GGCC'C
Cuts at: 0 494 612
Size: 494 118
ApaLI G'TGCA_C
Cuts at: 0 289 612
Size: 289 323
BamHI G'GATC_C
Cuts at: 0 35 562 612
Size: 35 527 50
Fragments arranged by size:
527 50 35
BanII G_rGCy'C
Cuts at: 0 494 612
Size: 494 118
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 58 92 612
Size: 58 34 520
Fragments arranged by size:
520 58 34
BlpI GC'TnA_GC
Cuts at: 0 498 612
Size: 498 114
Bme1580I G_kGCm'C
Cuts at: 0 293 494 612
Size: 293 201 118
Fragments arranged by size:
293 201 118
BmgBI CAC'GTC
Cuts at: 0 294 479 612
Size: 294 185 133
Fragments arranged by size:
294 185 133
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 181 612
Size: 181 431
BsaWI w'CCGG_w
Cuts at: 0 32 612
Size: 32 580
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 278 308 361 391 532 562 612
Size: 278 30 53 30 141 30 50
Fragments arranged by size:
278 141 53 50 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 97 451 538 612
Size: 97 354 87 74
Fragments arranged by size:
354 97 87 74
BseYI C'CCAG_C
Cuts at: 0 493 612
Size: 493 119
BsiHKAI G_wGCw'C
Cuts at: 0 293 411 612
Size: 293 118 201
Fragments arranged by size:
293 201 118
BsmBI CGTCTCn'nnnn_
Cuts at: 0 300 388 612
Size: 300 88 224
Fragments arranged by size:
300 224 88
Bsp1286I G_dGCh'C
Cuts at: 0 293 411 494 612
Size: 293 118 83 118
Fragments arranged by size:
293 118 118 83
BspEI T'CCGG_A
Cuts at: 0 32 612
Size: 32 580
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 363 612
Size: 363 249
BsrFI r'CCGG_y
Cuts at: 0 430 612
Size: 430 182
BsrGI T'GTAC_A
Cuts at: 0 116 612
Size: 116 496
BstYI r'GATC_y
Cuts at: 0 35 440 562 612
Size: 35 405 122 50
Fragments arranged by size:
405 122 50 35
BtsI GCAGTG_nn'
Cuts at: 0 337 612
Size: 337 275
EaeI y'GGCC_r
Cuts at: 0 101 612
Size: 101 511
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 181 612
Size: 181 431
EcoO109I rG'GnC_Cy
Cuts at: 0 490 612
Size: 490 122
EcoRV GAT'ATC
Cuts at: 0 150 612
Size: 150 462
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 178 210 278 310 612
Size: 178 32 68 32 302
Fragments arranged by size:
302 178 68 32 32
HincII GTy'rAC
Cuts at: 0 474 612
Size: 474 138
MscI TGG'CCA
Cuts at: 0 103 612
Size: 103 509
MslI CAynn'nnrTG
Cuts at: 0 556 612
Size: 556 56
PasI CC'CwG_GG
Cuts at: 0 181 612
Size: 181 431
PspOMI G'GGCC_C
Cuts at: 0 490 612
Size: 490 122
PstI C_TGCA'G
Cuts at: 0 277 344 612
Size: 277 67 268
Fragments arranged by size:
277 268 67
SfcI C'TryA_G
Cuts at: 0 273 340 612
Size: 273 67 272
Fragments arranged by size:
273 272 67
SgrAI Cr'CCGG_yG
Cuts at: 0 430 612
Size: 430 182
StuI AGG'CCT
Cuts at: 0 581 612
Size: 581 31
StyI C'CwwG_G
Cuts at: 0 43 612
Size: 43 569
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 369 612
Size: 369 243
TatI w'GTAC_w
Cuts at: 0 116 612
Size: 116 496
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 361 393 612
Size: 361 32 219
Fragments arranged by size:
361 219 32
Tth111I GACn'n_nGTC
Cuts at: 0 28 612
Size: 28 584
Enzymes that do cut:
AarI AflIII AleI ApaI ApaLI BamHI BanII BcgI
BlpI Bme1580I BmgBI BpmI BsaWI BsaXI BseRI BseYI
BsiHKAI BsmBI Bsp1286I BspEI BspMI BsrFI BsrGI BstYI
BtsI EaeI Eco57MI EcoO109I EcoRV Hin4I HincII MscI
MslI PasI PspOMI PstI SfcI SgrAI StuI StyI
TaqII TatI TstI Tth111I
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AgeI
AhdI AlfI AloI AlwNI ApoI AscI AseI AsiSI
AvaI AvrII BaeI BanI BbeI BbsI BbvCI BciVI
BclI BfrBI BglI BglII BmrI BmtI BplI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsgI BsiEI BsiWI
BsmI BspHI BsrBI BsrDI BssHII BssSI BstAPI BstBI
BstEII BstXI BstZ17I Bsu36I BtgI BtgZI ClaI CspCI
DraI DraIII DrdI EagI EarI EciI EcoICRI EcoNI
EcoRI FalI FseI FspI FspAI HaeII HindIII HpaI
KasI KpnI MfeI MluI MmeI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI
PacI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspXI PsrI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfiI
SfoI SgfI SmaI SmlI SnaBI SpeI SphI SrfI
SspI SwaI XbaI XcmI XhoI XmaI XmnI ZraI