Restriction map for
pF1KA1667
(Linear) (Six-base) MAPSORT of: KIAA1667.seq Check: 1014 from: 1 to: 2124
KIAA1667.orf
With 182 enzymes: SgfI *
March 3, 2008 10:55 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1848 2031 2124
Size: 1848 183 93
Fragments arranged by size:
1848 183 93
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 259 541 2052 2122 2124
Size: 259 282 1511 70 2
Fragments arranged by size:
1511 282 259 70 2
AfeI AGC'GCT
Cuts at: 0 553 2124
Size: 553 1571
AflIII A'CryG_T
Cuts at: 0 1170 2124
Size: 1170 954
AhdI GACnn_n'nnGTC
Cuts at: 0 598 2124
Size: 598 1526
AleI CACnn'nnGTG
Cuts at: 0 1969 2124
Size: 1969 155
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1046 1617 2124
Size: 1046 571 507
Fragments arranged by size:
1046 571 507
AlwNI CAG_nnn'CTG
Cuts at: 0 135 1510 2016 2097 2124
Size: 135 1375 506 81 27
Fragments arranged by size:
1375 506 135 81 27
ApoI r'AATT_y
Cuts at: 0 434 1915 2124
Size: 434 1481 209
Fragments arranged by size:
1481 434 209
BanI G'GyrC_C
Cuts at: 0 2019 2062 2124
Size: 2019 43 62
Fragments arranged by size:
2019 62 43
BanII G_rGCy'C
Cuts at: 0 299 509 1197 1317 1613 2038 2124
Size: 299 210 688 120 296 425 86
Fragments arranged by size:
688 425 299 296 210 120 86
BbeI G_GCGC'C
Cuts at: 0 2066 2124
Size: 2066 58
BbsI GAAGACnn'nnnn_
Cuts at: 0 1337 2124
Size: 1337 787
BbvCI CC'TCA_GC
Cuts at: 0 1054 1670 2124
Size: 1054 616 454
Fragments arranged by size:
1054 616 454
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1593 1627 2124
Size: 1593 34 497
Fragments arranged by size:
1593 497 34
BfrBI ATG'CAT
Cuts at: 0 1908 2124
Size: 1908 216
BglI GCCn_nnn'nGGC
Cuts at: 0 1966 2078 2124
Size: 1966 112 46
Fragments arranged by size:
1966 112 46
BlpI GC'TnA_GC
Cuts at: 0 1533 2124
Size: 1533 591
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1803 2124
Size: 1803 321
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 194 800 811 1018 1209 1563 1873 2124
Size: 194 606 11 207 191 354 310 251
Fragments arranged by size:
606 354 310 251 207 194 191 11
Bpu10I CC'TnA_GC
Cuts at: 0 1054 1130 1670 2124
Size: 1054 76 540 454
Fragments arranged by size:
1054 540 454 76
BsaI GGTCTCn'nnnn_
Cuts at: 0 809 2124
Size: 809 1315
BsaHI Gr'CG_yC
Cuts at: 0 2063 2124
Size: 2063 61
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 496 526 2124
Size: 496 30 1598
Fragments arranged by size:
1598 496 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 200 1050 1687 2124
Size: 200 850 637 437
Fragments arranged by size:
850 637 437 200
BseYI C'CCAG_C
Cuts at: 0 279 842 1304 1683 1922 2124
Size: 279 563 462 379 239 202
Fragments arranged by size:
563 462 379 279 239 202
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1006 2124
Size: 1006 1118
BsiEI CG_ry'CG
Cuts at: 0 1824 2124
Size: 1824 300
BsiHKAI G_wGCw'C
Cuts at: 0 299 409 868 2038 2124
Size: 299 110 459 1170 86
Fragments arranged by size:
1170 459 299 110 86
BsmI GAATG_Cn'
Cuts at: 0 533 1525 1826 2124
Size: 533 992 301 298
Fragments arranged by size:
992 533 301 298
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1756 2124
Size: 1756 368
Bsp1286I G_dGCh'C
Cuts at: 0 299 409 509 868 1197 1317 1613 2038
Size: 299 110 100 359 329 120 296 425
Cuts at: 2038 2124
Size: 86
Fragments arranged by size:
425 359 329 299 296 120 110 100
86
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 553 1314 1848 1859 2031 2124
Size: 553 761 534 11 172 93
Fragments arranged by size:
761 553 534 172 93 11
BsrBI CCG'CTC
Cuts at: 0 709 2124
Size: 709 1415
BsrDI GCAATG_nn'
Cuts at: 0 712 2124
Size: 712 1412
BssSI C'ACGA_G
Cuts at: 0 36 775 1612 1791 2124
Size: 36 739 837 179 333
Fragments arranged by size:
837 739 333 179 36
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2097 2124
Size: 2097 27
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1233 2124
Size: 1233 891
BstYI r'GATC_y
Cuts at: 0 927 1422 2124
Size: 927 495 702
Fragments arranged by size:
927 702 495
Bsu36I CC'TnA_GG
Cuts at: 0 659 1149 1250 2054 2124
Size: 659 490 101 804 70
Fragments arranged by size:
804 659 490 101 70
BtgI C'CryG_G
Cuts at: 0 908 1451 1964 2124
Size: 908 543 513 160
Fragments arranged by size:
908 543 513 160
BtsI GCAGTG_nn'
Cuts at: 0 1635 2124
Size: 1635 489
DraIII CAC_nnn'GTG
Cuts at: 0 413 2112 2124
Size: 413 1699 12
Fragments arranged by size:
1699 413 12
EaeI y'GGCC_r
Cuts at: 0 2 894 987 1341 1862 2124
Size: 2 892 93 354 521 262
Fragments arranged by size:
892 521 354 262 93 2
EcoICRI GAG'CTC
Cuts at: 0 297 2036 2124
Size: 297 1739 88
Fragments arranged by size:
1739 297 88
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 194 259 541 800 811 1018 1209 1563
Size: 194 65 282 259 11 207 191 354
Cuts at: 1563 1873 2052 2122 2124
Size: 310 179 70 2
Fragments arranged by size:
354 310 282 259 207 194 191 179
70 65 11 2
EcoNI CCTnn'n_nnAGG
Cuts at: 0 680 1069 1471 1855 2124
Size: 680 389 402 384 269
Fragments arranged by size:
680 402 389 384 269
EcoO109I rG'GnC_Cy
Cuts at: 0 636 1391 1400 2124
Size: 636 755 9 724
Fragments arranged by size:
755 724 636 9
HaeII r_GCGC'y
Cuts at: 0 555 1223 2066 2124
Size: 555 668 843 58
Fragments arranged by size:
843 668 555 58
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 13 45 584 616 789 821 919 951
Size: 13 32 539 32 173 32 98 32
Cuts at: 951 1608 1640 2124
Size: 657 32 484
Fragments arranged by size:
657 539 484 173 98 32 32 32
32 32 13
HindIII A'AGCT_T
Cuts at: 0 947 1444 2124
Size: 947 497 680
Fragments arranged by size:
947 680 497
KasI G'GCGC_C
Cuts at: 0 2062 2124
Size: 2062 62
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 118 318 2124
Size: 118 200 1806
Fragments arranged by size:
1806 200 118
MscI TGG'CCA
Cuts at: 0 4 896 989 1343 1864 2124
Size: 4 892 93 354 521 260
Fragments arranged by size:
892 521 354 260 93 4
MslI CAynn'nnrTG
Cuts at: 0 1969 2124
Size: 1969 155
MspA1I CmG'CkG
Cuts at: 0 185 311 1680 1926 2016 2124
Size: 185 126 1369 246 90 108
Fragments arranged by size:
1369 246 185 126 108 90
NarI GG'CG_CC
Cuts at: 0 2063 2124
Size: 2063 61
NcoI C'CATG_G
Cuts at: 0 908 2124
Size: 908 1216
NsiI A_TGCA'T
Cuts at: 0 1910 2124
Size: 1910 214
NspI r_CATG'y
Cuts at: 0 1174 2124
Size: 1174 950
PasI CC'CwG_GG
Cuts at: 0 1065 1772 2124
Size: 1065 707 352
Fragments arranged by size:
1065 707 352
PciI A'CATG_T
Cuts at: 0 1170 2124
Size: 1170 954
PflMI CCAn_nnn'nTGG
Cuts at: 0 859 914 996 2016 2124
Size: 859 55 82 1020 108
Fragments arranged by size:
1020 859 108 82 55
PfoI T'CCnGG_A
Cuts at: 0 827 1425 1994 2124
Size: 827 598 569 130
Fragments arranged by size:
827 598 569 130
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 642 674 2124
Size: 642 32 1450
Fragments arranged by size:
1450 642 32
PpuMI rG'GwC_Cy
Cuts at: 0 636 1391 2124
Size: 636 755 733
Fragments arranged by size:
755 733 636
PstI C_TGCA'G
Cuts at: 0 542 1566 2028 2124
Size: 542 1024 462 96
Fragments arranged by size:
1024 542 462 96
PvuII CAG'CTG
Cuts at: 0 311 1926 2016 2124
Size: 311 1615 90 108
Fragments arranged by size:
1615 311 108 90
SacI G_AGCT'C
Cuts at: 0 299 2038 2124
Size: 299 1739 86
Fragments arranged by size:
1739 299 86
SfcI C'TryA_G
Cuts at: 0 538 1562 2024 2124
Size: 538 1024 462 100
Fragments arranged by size:
1024 538 462 100
SfoI GGC'GCC
Cuts at: 0 2064 2124
Size: 2064 60
SpeI A'CTAG_T
Cuts at: 0 1077 2124
Size: 1077 1047
StuI AGG'CCT
Cuts at: 0 1466 2124
Size: 1466 658
StyI C'CwwG_G
Cuts at: 0 71 632 908 2124
Size: 71 561 276 1216
Fragments arranged by size:
1216 561 276 71
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 264 622 2124
Size: 264 358 1502
Fragments arranged by size:
1502 358 264
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 912 944 2124
Size: 912 32 1180
Fragments arranged by size:
1180 912 32
Tth111I GACn'n_nGTC
Cuts at: 0 801 2124
Size: 801 1323
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 856 897 1507 2124
Size: 856 41 610 617
Fragments arranged by size:
856 617 610 41
Enzymes that do cut:
AarI AcuI AfeI AflIII AhdI AleI AlfI AlwNI
ApoI BanI BanII BbeI BbsI BbvCI BcgI BfrBI
BglI BlpI BplI BpmI Bpu10I BsaI BsaHI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspMI BsrBI BsrDI BssSI BstAPI BstXI BstYI Bsu36I
BtgI BtsI DraIII EaeI EcoICRI Eco57MI EcoNI EcoO109I
HaeII Hin4I HindIII KasI MmeI MscI MslI MspA1I
NarI NcoI NsiI NspI PasI PciI PflMI PfoI
PpiI PpuMI PstI PvuII SacI SfcI SfoI SpeI
StuI StyI TaqII TstI Tth111I XcmI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AgeI AloI ApaI
ApaLI AscI AseI AsiSI AvaI AvrII BaeI BamHI
BciVI BclI BglII Bme1580I BmgBI BmrI BmtI BpuEI
BsaAI BsaBI BsaWI BsiWI BspEI BspHI BsrFI BsrGI
BssHII BstBI BstEII BstZ17I BtgZI ClaI CspCI DraI
DrdI EagI EarI EciI EcoRI EcoRV FalI FseI
FspI FspAI HincII HpaI KpnI MfeI MluI NaeI
NdeI NgoMIV NheI NotI NruI PacI PmeI PmlI
PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacII
SalI SanDI SapI SbfI ScaI SexAI SfiI SgfI
SgrAI SmaI SmlI SnaBI SphI SrfI SspI SwaI
TatI XbaI XhoI XmaI XmnI ZraI