Restriction map for
pF1KA1673
(Linear) (Six-base) MAPSORT of: KIAA1673.seq Check: 6469 from: 1 to: 2136
KIAA1673.orf
With 182 enzymes: SgfI *
October 24, 2006 15:09 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1308 2136
Size: 1308 828
AlwNI CAG_nnn'CTG
Cuts at: 0 509 2136
Size: 509 1627
ApaI G_GGCC'C
Cuts at: 0 1968 2136
Size: 1968 168
ApoI r'AATT_y
Cuts at: 0 624 1008 1981 2071 2136
Size: 624 384 973 90 65
Fragments arranged by size:
973 624 384 90 65
AvaI C'yCGr_G
Cuts at: 0 900 2136
Size: 900 1236
BanII G_rGCy'C
Cuts at: 0 901 1968 2136
Size: 901 1067 168
Fragments arranged by size:
1067 901 168
BbsI GAAGACnn'nnnn_
Cuts at: 0 1229 2136
Size: 1229 907
BclI T'GATC_A
Cuts at: 0 1938 2136
Size: 1938 198
BfrBI ATG'CAT
Cuts at: 0 1501 1549 1553 2136
Size: 1501 48 4 583
Fragments arranged by size:
1501 583 48 4
BglII A'GATC_T
Cuts at: 0 218 2136
Size: 218 1918
Bme1580I G_kGCm'C
Cuts at: 0 1872 1968 2136
Size: 1872 96 168
Fragments arranged by size:
1872 168 96
BmrI ACTGGGnnnn_n'
Cuts at: 0 50 455 2136
Size: 50 405 1681
Fragments arranged by size:
1681 405 50
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 176 395 915 1018 1177 1373 2136
Size: 176 219 520 103 159 196 763
Fragments arranged by size:
763 520 219 196 176 159 103
Bpu10I CC'TnA_GC
Cuts at: 0 1796 2136
Size: 1796 340
BsaI GGTCTCn'nnnn_
Cuts at: 0 758 2136
Size: 758 1378
BsaWI w'CCGG_w
Cuts at: 0 1122 2136
Size: 1122 1014
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 11 41 2136
Size: 11 30 2095
Fragments arranged by size:
2095 30 11
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 525 659 876 2136
Size: 525 134 217 1260
Fragments arranged by size:
1260 525 217 134
BseYI C'CCAG_C
Cuts at: 0 115 166 923 1051 1777 1819 2039 2136
Size: 115 51 757 128 726 42 220 97
Fragments arranged by size:
757 726 220 128 115 97 51 42
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1552 2136
Size: 1552 584
BsiHKAI G_wGCw'C
Cuts at: 0 612 2136
Size: 612 1524
BsmI GAATG_Cn'
Cuts at: 0 1501 2136
Size: 1501 635
BsmBI CGTCTCn'nnnn_
Cuts at: 0 494 2136
Size: 494 1642
Bsp1286I G_dGCh'C
Cuts at: 0 612 901 1872 1968 2136
Size: 612 289 971 96 168
Fragments arranged by size:
971 612 289 168 96
BspEI T'CCGG_A
Cuts at: 0 1122 2136
Size: 1122 1014
BsrDI GCAATG_nn'
Cuts at: 0 387 2136
Size: 387 1749
BssSI C'ACGA_G
Cuts at: 0 2057 2136
Size: 2057 79
BstEII G'GTnAC_C
Cuts at: 0 2101 2136
Size: 2101 35
BstYI r'GATC_y
Cuts at: 0 218 2136
Size: 218 1918
BtgI C'CryG_G
Cuts at: 0 850 1278 2136
Size: 850 428 858
Fragments arranged by size:
858 850 428
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1753 2136
Size: 1753 383
BtsI GCAGTG_nn'
Cuts at: 0 168 1317 2136
Size: 168 1149 819
Fragments arranged by size:
1149 819 168
EaeI y'GGCC_r
Cuts at: 0 1275 2136
Size: 1275 861
EarI CTCTTCn'nnn_
Cuts at: 0 894 1298 2136
Size: 894 404 838
Fragments arranged by size:
894 838 404
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1394 2136
Size: 1394 742
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 176 395 915 1018 1177 1308 1373 2136
Size: 176 219 520 103 159 131 65 763
Fragments arranged by size:
763 520 219 176 159 131 103 65
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1490 2089 2136
Size: 1490 599 47
Fragments arranged by size:
1490 599 47
EcoO109I rG'GnC_Cy
Cuts at: 0 582 1964 2136
Size: 582 1382 172
Fragments arranged by size:
1382 582 172
EcoRI G'AATT_C
Cuts at: 0 2071 2136
Size: 2071 65
HaeII r_GCGC'y
Cuts at: 0 529 2136
Size: 529 1607
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 393 425 666 698 1135 1167 1459 1491
Size: 393 32 241 32 437 32 292 32
Cuts at: 1491 1510 1542 1734 1766 2136
Size: 19 32 192 32 370
Fragments arranged by size:
437 393 370 292 241 192 32 32
32 32 32 32 19
HincII GTy'rAC
Cuts at: 0 419 2136
Size: 419 1717
MslI CAynn'nnrTG
Cuts at: 0 104 791 1334 2057 2136
Size: 104 687 543 723 79
Fragments arranged by size:
723 687 543 104 79
MspA1I CmG'CkG
Cuts at: 0 148 810 1884 1944 2129 2136
Size: 148 662 1074 60 185 7
Fragments arranged by size:
1074 662 185 148 60 7
NdeI CA'TA_TG
Cuts at: 0 1215 1926 2136
Size: 1215 711 210
Fragments arranged by size:
1215 711 210
NsiI A_TGCA'T
Cuts at: 0 1503 1551 1555 2136
Size: 1503 48 4 581
Fragments arranged by size:
1503 581 48 4
NspI r_CATG'y
Cuts at: 0 1149 1553 2136
Size: 1149 404 583
Fragments arranged by size:
1149 583 404
PfoI T'CCnGG_A
Cuts at: 0 892 1075 2136
Size: 892 183 1061
Fragments arranged by size:
1061 892 183
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 976 1008 2136
Size: 976 32 1128
Fragments arranged by size:
1128 976 32
PpuMI rG'GwC_Cy
Cuts at: 0 582 2136
Size: 582 1554
PsiI TTA'TAA
Cuts at: 0 133 2136
Size: 133 2003
PspOMI G'GGCC_C
Cuts at: 0 1964 2136
Size: 1964 172
PstI C_TGCA'G
Cuts at: 0 86 1889 2021 2136
Size: 86 1803 132 115
Fragments arranged by size:
1803 132 115 86
PvuII CAG'CTG
Cuts at: 0 148 810 1884 1944 2136
Size: 148 662 1074 60 192
Fragments arranged by size:
1074 662 192 148 60
SanDI GG'GwC_CC
Cuts at: 0 582 2136
Size: 582 1554
SfcI C'TryA_G
Cuts at: 0 82 1885 2017 2136
Size: 82 1803 132 119
Fragments arranged by size:
1803 132 119 82
SmaI CCC'GGG
Cuts at: 0 902 2136
Size: 902 1234
SphI G_CATG'C
Cuts at: 0 1553 2136
Size: 1553 583
SrfI GCCC'GGGC
Cuts at: 0 902 2136
Size: 902 1234
SspI AAT'ATT
Cuts at: 0 2035 2136
Size: 2035 101
StyI C'CwwG_G
Cuts at: 0 936 1627 2136
Size: 936 691 509
Fragments arranged by size:
936 691 509
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 713 2136
Size: 713 1423
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 11 43 1623 1655 2136
Size: 11 32 1580 32 481
Fragments arranged by size:
1580 481 32 32 11
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2059 2136
Size: 2059 77
XmaI C'CCGG_G
Cuts at: 0 900 2136
Size: 900 1236
Enzymes that do cut:
AcuI AlwNI ApaI ApoI AvaI BanII BbsI BclI
BfrBI BglII Bme1580I BmrI BpmI Bpu10I BsaI BsaWI
BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BsrDI BssSI BstEII BstYI BtgI BtgZI BtsI
EaeI EarI EciI Eco57MI EcoNI EcoO109I EcoRI HaeII
Hin4I HincII MslI MspA1I NdeI NsiI NspI PfoI
PpiI PpuMI PsiI PspOMI PstI PvuII SanDI SfcI
SmaI SphI SrfI SspI StyI TaqII TstI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaLI AscI AseI
AsiSI AvrII BaeI BamHI BanI BbeI BbvCI BcgI
BciVI BglI BlpI BmgBI BmtI BplI BpuEI BsaAI
BsaBI BsaHI BsiEI BsiWI BspHI BspMI BsrBI BsrFI
BsrGI BssHII BstAPI BstBI BstXI BstZ17I Bsu36I ClaI
CspCI DraI DraIII DrdI EagI EcoICRI EcoRV FalI
FseI FspI FspAI HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI NaeI NarI NcoI NgoMIV NheI
NotI NruI PacI PasI PciI PflMI PmeI PmlI
PshAI PspXI PsrI PvuI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmlI SnaBI SpeI StuI SwaI TatI Tth111I XbaI
XhoI XmnI ZraI