Restriction map for
pF1KA1677
(Linear) (Six-base) MAPSORT of: KIAA1677.seq Check: 491 from: 1 to: 1812
KIAA1677.orf
With 182 enzymes: SgfI *
October 24, 2006 15:09 ..
AccI GT'mk_AC
Cuts at: 0 1541 1812
Size: 1541 271
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 150 298 1812
Size: 150 148 1514
Fragments arranged by size:
1514 150 148
AgeI A'CCGG_T
Cuts at: 0 238 1291 1812
Size: 238 1053 521
Fragments arranged by size:
1053 521 238
AhdI GACnn_n'nnGTC
Cuts at: 0 513 1812
Size: 513 1299
AlwNI CAG_nnn'CTG
Cuts at: 0 1555 1812
Size: 1555 257
ApaI G_GGCC'C
Cuts at: 0 1015 1812
Size: 1015 797
ApoI r'AATT_y
Cuts at: 0 212 718 1024 1111 1219 1812
Size: 212 506 306 87 108 593
Fragments arranged by size:
593 506 306 212 108 87
AvaI C'yCGr_G
Cuts at: 0 1059 1812
Size: 1059 753
BamHI G'GATC_C
Cuts at: 0 1227 1812
Size: 1227 585
BanII G_rGCy'C
Cuts at: 0 1015 1812
Size: 1015 797
BbsI GAAGACnn'nnnn_
Cuts at: 0 633 1467 1812
Size: 633 834 345
Fragments arranged by size:
834 633 345
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 941 975 1812
Size: 941 34 837
Fragments arranged by size:
941 837 34
BclI T'GATC_A
Cuts at: 0 558 1557 1812
Size: 558 999 255
Fragments arranged by size:
999 558 255
BglII A'GATC_T
Cuts at: 0 380 1812
Size: 380 1432
Bme1580I G_kGCm'C
Cuts at: 0 1015 1812
Size: 1015 797
BmgBI CAC'GTC
Cuts at: 0 12 1812
Size: 12 1800
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 654 1812
Size: 654 1158
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1367 1518 1812
Size: 1367 151 294
Fragments arranged by size:
1367 294 151
BsaBI GATnn'nnATC
Cuts at: 0 557 1232 1812
Size: 557 675 580
Fragments arranged by size:
675 580 557
BsaWI w'CCGG_w
Cuts at: 0 238 665 1291 1692 1776 1812
Size: 238 427 626 401 84 36
Fragments arranged by size:
626 427 401 238 84 36
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 91 1812
Size: 91 1721
BseYI C'CCAG_C
Cuts at: 0 55 1008 1342 1812
Size: 55 953 334 470
Fragments arranged by size:
953 470 334 55
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 419 622 1812
Size: 419 203 1190
Fragments arranged by size:
1190 419 203
BsiHKAI G_wGCw'C
Cuts at: 0 69 1271 1812
Size: 69 1202 541
Fragments arranged by size:
1202 541 69
Bsp1286I G_dGCh'C
Cuts at: 0 69 1015 1271 1812
Size: 69 946 256 541
Fragments arranged by size:
946 541 256 69
BspEI T'CCGG_A
Cuts at: 0 1692 1776 1812
Size: 1692 84 36
Fragments arranged by size:
1692 84 36
BspHI T'CATG_A
Cuts at: 0 303 1812
Size: 303 1509
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 560 1775 1812
Size: 560 1215 37
Fragments arranged by size:
1215 560 37
BsrFI r'CCGG_y
Cuts at: 0 238 1291 1812
Size: 238 1053 521
Fragments arranged by size:
1053 521 238
BstBI TT'CG_AA
Cuts at: 0 1511 1812
Size: 1511 301
BstEII G'GTnAC_C
Cuts at: 0 1389 1812
Size: 1389 423
BstXI CCAn_nnnn'nTGG
Cuts at: 0 917 1812
Size: 917 895
BstYI r'GATC_y
Cuts at: 0 380 1227 1812
Size: 380 847 585
Fragments arranged by size:
847 585 380
BstZ17I GTA'TAC
Cuts at: 0 1542 1812
Size: 1542 270
BtgI C'CryG_G
Cuts at: 0 1602 1812
Size: 1602 210
DraI TTT'AAA
Cuts at: 0 221 1812
Size: 221 1591
DrdI GACnn_nn'nnGTC
Cuts at: 0 46 1812
Size: 46 1766
EaeI y'GGCC_r
Cuts at: 0 146 1599 1812
Size: 146 1453 213
Fragments arranged by size:
1453 213 146
EarI CTCTTCn'nnn_
Cuts at: 0 998 1812
Size: 998 814
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 150 298 654 1812
Size: 150 148 356 1158
Fragments arranged by size:
1158 356 150 148
EcoO109I rG'GnC_Cy
Cuts at: 0 1502 1812
Size: 1502 310
EcoRI G'AATT_C
Cuts at: 0 1024 1812
Size: 1024 788
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 499 531 796 828 1184 1216 1812
Size: 499 32 265 32 356 32 596
Fragments arranged by size:
596 499 356 265 32 32 32
HincII GTy'rAC
Cuts at: 0 756 882 1242 1812
Size: 756 126 360 570
Fragments arranged by size:
756 570 360 126
HindIII A'AGCT_T
Cuts at: 0 1465 1812
Size: 1465 347
HpaI GTT'AAC
Cuts at: 0 882 1242 1812
Size: 882 360 570
Fragments arranged by size:
882 570 360
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 782 1812
Size: 782 1030
MscI TGG'CCA
Cuts at: 0 148 1601 1812
Size: 148 1453 211
Fragments arranged by size:
1453 211 148
NcoI C'CATG_G
Cuts at: 0 1602 1812
Size: 1602 210
PflMI CCAn_nnn'nTGG
Cuts at: 0 1400 1812
Size: 1400 412
PsiI TTA'TAA
Cuts at: 0 1655 1812
Size: 1655 157
PspOMI G'GGCC_C
Cuts at: 0 1011 1812
Size: 1011 801
PstI C_TGCA'G
Cuts at: 0 186 1082 1812
Size: 186 896 730
Fragments arranged by size:
896 730 186
SapI GCTCTTCn'nnn_
Cuts at: 0 998 1812
Size: 998 814
SfcI C'TryA_G
Cuts at: 0 182 278 845 1078 1118 1532 1812
Size: 182 96 567 233 40 414 280
Fragments arranged by size:
567 414 280 233 182 96 40
SgrAI Cr'CCGG_yG
Cuts at: 0 1291 1812
Size: 1291 521
SmlI C'TyrA_G
Cuts at: 0 1382 1497 1812
Size: 1382 115 315
Fragments arranged by size:
1382 315 115
StuI AGG'CCT
Cuts at: 0 504 1646 1812
Size: 504 1142 166
Fragments arranged by size:
1142 504 166
StyI C'CwwG_G
Cuts at: 0 1602 1812
Size: 1602 210
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 166 1812
Size: 166 1646
TatI w'GTAC_w
Cuts at: 0 266 1785 1812
Size: 266 1519 27
Fragments arranged by size:
1519 266 27
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1674 1706 1812
Size: 1674 32 106
Fragments arranged by size:
1674 106 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 917 1812
Size: 917 895
Enzymes that do cut:
AccI AcuI AgeI AhdI AlwNI ApaI ApoI AvaI
BamHI BanII BbsI BcgI BclI BglII Bme1580I BmgBI
BpmI BpuEI BsaBI BsaWI BseRI BseYI BsgI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrFI BstBI BstEII BstXI
BstYI BstZ17I BtgI DraI DrdI EaeI EarI Eco57MI
EcoO109I EcoRI Hin4I HincII HindIII HpaI MmeI MscI
NcoI PflMI PsiI PspOMI PstI SapI SfcI SgrAI
SmlI StuI StyI TaqII TatI TstI XcmI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AleI
AlfI AloI ApaLI AscI AseI AsiSI AvrII BaeI
BanI BbeI BbvCI BciVI BfrBI BglI BlpI BmrI
BmtI BplI Bpu10I BsaI BsaAI BsaHI BsaXI BsiEI
BsiWI BsmI BsmBI BsrBI BsrDI BsrGI BssHII BssSI
BstAPI Bsu36I BtgZI BtsI ClaI CspCI DraIII EagI
EciI EcoICRI EcoNI EcoRV FalI FseI FspI FspAI
HaeII KasI KpnI MfeI MluI MslI MspA1I NaeI
NarI NdeI NgoMIV NheI NotI NruI NsiI NspI
PacI PasI PciI PfoI PmeI PmlI PpiI PpuMI
PshAI PspXI PsrI PvuI PvuII RsrII SacI SacII
SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI
SmaI SnaBI SpeI SphI SrfI SspI SwaI Tth111I
XbaI XhoI XmaI XmnI ZraI