Restriction map for
pF1KA1680
(Linear) (Six-base) MAPSORT of: KIAA1680.seq Check: 4627 from: 1 to: 2700
KIAA1680.orf
With 182 enzymes: SgfI *
March 3, 2008 10:55 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 755 812 2009 2065 2401 2426 2509 2700
Size: 755 57 1197 56 336 25 83 191
Fragments arranged by size:
1197 755 336 191 83 57 56 25
AflIII A'CryG_T
Cuts at: 0 1215 2621 2700
Size: 1215 1406 79
Fragments arranged by size:
1406 1215 79
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2689 2700
Size: 2689 11
AlwNI CAG_nnn'CTG
Cuts at: 0 306 2700
Size: 306 2394
ApoI r'AATT_y
Cuts at: 0 568 622 775 1570 1702 2700
Size: 568 54 153 795 132 998
Fragments arranged by size:
998 795 568 153 132 54
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 601 634 2700
Size: 601 33 2066
Fragments arranged by size:
2066 601 33
BamHI G'GATC_C
Cuts at: 0 1226 2700
Size: 1226 1474
BanII G_rGCy'C
Cuts at: 0 2485 2516 2700
Size: 2485 31 184
Fragments arranged by size:
2485 184 31
BbsI GAAGACnn'nnnn_
Cuts at: 0 78 345 503 1582 2700
Size: 78 267 158 1079 1118
Fragments arranged by size:
1118 1079 267 158 78
BclI T'GATC_A
Cuts at: 0 1269 1755 2700
Size: 1269 486 945
Fragments arranged by size:
1269 945 486
BfrBI ATG'CAT
Cuts at: 0 817 2700
Size: 817 1883
BglII A'GATC_T
Cuts at: 0 385 2700
Size: 385 2315
BmgBI CAC'GTC
Cuts at: 0 1964 2700
Size: 1964 736
Bpu10I CC'TnA_GC
Cuts at: 0 156 233 1709 2700
Size: 156 77 1476 991
Fragments arranged by size:
1476 991 156 77
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 135 2023 2700
Size: 135 1888 677
Fragments arranged by size:
1888 677 135
BsaI GGTCTCn'nnnn_
Cuts at: 0 39 2700
Size: 39 2661
BsaWI w'CCGG_w
Cuts at: 0 1011 2700
Size: 1011 1689
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 192 1386 2700
Size: 192 1194 1314
Fragments arranged by size:
1314 1194 192
BseYI C'CCAG_C
Cuts at: 0 1155 2091 2700
Size: 1155 936 609
Fragments arranged by size:
1155 936 609
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1866 1955 2700
Size: 1866 89 745
Fragments arranged by size:
1866 745 89
BsiHKAI G_wGCw'C
Cuts at: 0 279 422 2485 2700
Size: 279 143 2063 215
Fragments arranged by size:
2063 279 215 143
BsmI GAATG_Cn'
Cuts at: 0 1793 2032 2565 2700
Size: 1793 239 533 135
Fragments arranged by size:
1793 533 239 135
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1638 2700
Size: 1638 1062
Bsp1286I G_dGCh'C
Cuts at: 0 279 422 2485 2516 2700
Size: 279 143 2063 31 184
Fragments arranged by size:
2063 279 184 143 31
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1074 1468 2573 2700
Size: 1074 394 1105 127
Fragments arranged by size:
1105 1074 394 127
BsrGI T'GTAC_A
Cuts at: 0 2624 2700
Size: 2624 76
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1315 2700
Size: 1315 1385
BstXI CCAn_nnnn'nTGG
Cuts at: 0 606 2695 2700
Size: 606 2089 5
Fragments arranged by size:
2089 606 5
BstYI r'GATC_y
Cuts at: 0 385 1226 1528 2700
Size: 385 841 302 1172
Fragments arranged by size:
1172 841 385 302
BtsI GCAGTG_nn'
Cuts at: 0 1397 2700
Size: 1397 1303
EaeI y'GGCC_r
Cuts at: 0 861 2700
Size: 861 1839
EarI CTCTTCn'nnn_
Cuts at: 0 89 1909 1985 2081 2700
Size: 89 1820 76 96 619
Fragments arranged by size:
1820 619 96 89 76
EcoICRI GAG'CTC
Cuts at: 0 2483 2700
Size: 2483 217
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 755 812 2009 2065 2401 2426 2509 2700
Size: 755 57 1197 56 336 25 83 191
Fragments arranged by size:
1197 755 336 191 83 57 56 25
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1820 2700
Size: 1820 880
EcoRI G'AATT_C
Cuts at: 0 622 2700
Size: 622 2078
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 456 2441 2700
Size: 456 1985 259
Fragments arranged by size:
1985 456 259
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 30 787 819 862 894 2014 2046 2232
Size: 30 757 32 43 32 1120 32 186
Cuts at: 2232 2264 2594 2626 2700
Size: 32 330 32 74
Fragments arranged by size:
1120 757 330 186 74 43 32 32
32 32 32 30
HincII GTy'rAC
Cuts at: 0 527 2350 2700
Size: 527 1823 350
Fragments arranged by size:
1823 527 350
MscI TGG'CCA
Cuts at: 0 863 2700
Size: 863 1837
MslI CAynn'nnrTG
Cuts at: 0 1301 2700
Size: 1301 1399
MspA1I CmG'CkG
Cuts at: 0 2049 2314 2700
Size: 2049 265 386
Fragments arranged by size:
2049 386 265
NsiI A_TGCA'T
Cuts at: 0 819 2700
Size: 819 1881
NspI r_CATG'y
Cuts at: 0 1219 2625 2700
Size: 1219 1406 75
Fragments arranged by size:
1406 1219 75
PciI A'CATG_T
Cuts at: 0 1215 2621 2700
Size: 1215 1406 79
Fragments arranged by size:
1406 1215 79
PshAI GACnn'nnGTC
Cuts at: 0 1943 2700
Size: 1943 757
PsiI TTA'TAA
Cuts at: 0 445 2700
Size: 445 2255
PstI C_TGCA'G
Cuts at: 0 1404 2048 2700
Size: 1404 644 652
Fragments arranged by size:
1404 652 644
PvuII CAG'CTG
Cuts at: 0 2049 2700
Size: 2049 651
SacI G_AGCT'C
Cuts at: 0 2485 2700
Size: 2485 215
SapI GCTCTTCn'nnn_
Cuts at: 0 1985 2081 2700
Size: 1985 96 619
Fragments arranged by size:
1985 619 96
SexAI A'CCwGG_T
Cuts at: 0 282 2700
Size: 282 2418
SfcI C'TryA_G
Cuts at: 0 586 1400 2044 2700
Size: 586 814 644 656
Fragments arranged by size:
814 656 644 586
SmlI C'TyrA_G
Cuts at: 0 150 2038 2700
Size: 150 1888 662
Fragments arranged by size:
1888 662 150
SspI AAT'ATT
Cuts at: 0 50 2700
Size: 50 2650
StyI C'CwwG_G
Cuts at: 0 1171 2700
Size: 1171 1529
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2613 2700
Size: 2613 87
TatI w'GTAC_w
Cuts at: 0 2199 2624 2700
Size: 2199 425 76
Fragments arranged by size:
2199 425 76
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1953 1985 2700
Size: 1953 32 715
Fragments arranged by size:
1953 715 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1808 2700
Size: 1808 892
XmnI GAAnn'nnTTC
Cuts at: 0 342 2700
Size: 342 2358
Enzymes that do cut:
AcuI AflIII AlfI AlwNI ApoI BaeI BamHI BanII
BbsI BclI BfrBI BglII BmgBI Bpu10I BpuEI BsaI
BsaWI BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I
BspMI BsrGI BstAPI BstXI BstYI BtsI EaeI EarI
EcoICRI Eco57MI EcoNI EcoRI FalI Hin4I HincII MscI
MslI MspA1I NsiI NspI PciI PshAI PsiI PstI
PvuII SacI SapI SexAI SfcI SmlI SspI StyI
TaqII TatI TstI XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AloI ApaI ApaLI AscI AseI AsiSI
AvaI AvrII BanI BbeI BbvCI BcgI BciVI BglI
BlpI Bme1580I BmrI BmtI BplI BpmI BsaAI BsaBI
BsaHI BsaXI BsiEI BsiWI BspEI BspHI BsrBI BsrDI
BsrFI BssHII BssSI BstBI BstEII BstZ17I Bsu36I BtgI
BtgZI ClaI CspCI DraI DraIII DrdI EagI EciI
EcoO109I EcoRV FseI FspI FspAI HaeII HindIII HpaI
KasI KpnI MfeI MluI MmeI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI PacI PasI PflMI
PfoI PmeI PmlI PpiI PpuMI PspOMI PspXI PsrI
PvuI RsrII SacII SalI SanDI SbfI ScaI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
StuI SwaI Tth111I XbaI XhoI XmaI ZraI