Restriction map for
pF1KSDA1686
(Linear) (Six-base) MAPSORT of: KIAA1686.seq Check: 7290 from: 1 to: 3522
KIAA1686.orf
With 182 enzymes: SgfI *
October 24, 2006 15:17 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 353 377 698 1385 2084 2125 2666 2945
Size: 353 24 321 687 699 41 541 279
Cuts at: 2945 3132 3353 3522
Size: 187 221 169
Fragments arranged by size:
699 687 541 353 321 279 221 187
169 41 24
AflIII A'CryG_T
Cuts at: 0 2284 3522
Size: 2284 1238
AgeI A'CCGG_T
Cuts at: 0 504 3522
Size: 504 3018
AhdI GACnn_n'nnGTC
Cuts at: 0 1782 3522
Size: 1782 1740
AleI CACnn'nnGTG
Cuts at: 0 2402 3522
Size: 2402 1120
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 187 3522
Size: 187 3335
AlwNI CAG_nnn'CTG
Cuts at: 0 817 2662 3522
Size: 817 1845 860
Fragments arranged by size:
1845 860 817
ApaI G_GGCC'C
Cuts at: 0 1187 1749 3522
Size: 1187 562 1773
Fragments arranged by size:
1773 1187 562
ApaLI G'TGCA_C
Cuts at: 0 1090 2438 3522
Size: 1090 1348 1084
Fragments arranged by size:
1348 1090 1084
ApoI r'AATT_y
Cuts at: 0 477 1038 1224 1970 2429 2465 2991 3219
Size: 477 561 186 746 459 36 526 228
Cuts at: 3219 3522
Size: 303
Fragments arranged by size:
746 561 526 477 459 303 228 186
36
AseI AT'TA_AT
Cuts at: 0 689 1022 3522
Size: 689 333 2500
Fragments arranged by size:
2500 689 333
AvaI C'yCGr_G
Cuts at: 0 2300 3522
Size: 2300 1222
BanII G_rGCy'C
Cuts at: 0 421 1187 1344 1749 3522
Size: 421 766 157 405 1773
Fragments arranged by size:
1773 766 421 405 157
BbsI GAAGACnn'nnnn_
Cuts at: 0 69 1691 2910 3522
Size: 69 1622 1219 612
Fragments arranged by size:
1622 1219 612 69
BciVI GTATCCnnnnn_n'
Cuts at: 0 637 1657 1915 3522
Size: 637 1020 258 1607
Fragments arranged by size:
1607 1020 637 258
BfrBI ATG'CAT
Cuts at: 0 2211 3522
Size: 2211 1311
BglI GCCn_nnn'nGGC
Cuts at: 0 425 3522
Size: 425 3097
BglII A'GATC_T
Cuts at: 0 1152 3522
Size: 1152 2370
Bme1580I G_kGCm'C
Cuts at: 0 1094 1187 1749 2442 3522
Size: 1094 93 562 693 1080
Fragments arranged by size:
1094 1080 693 562 93
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 45 3522
Size: 45 3477
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 40 3522
Size: 40 3482
Bpu10I CC'TnA_GC
Cuts at: 0 1757 3203 3522
Size: 1757 1446 319
Fragments arranged by size:
1757 1446 319
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1131 1966 2191 2799 3272 3522
Size: 1131 835 225 608 473 250
Fragments arranged by size:
1131 835 608 473 250 225
BsaAI yAC'GTr
Cuts at: 0 2285 3522
Size: 2285 1237
BsaBI GATnn'nnATC
Cuts at: 0 1255 3522
Size: 1255 2267
BsaWI w'CCGG_w
Cuts at: 0 145 504 3522
Size: 145 359 3018
Fragments arranged by size:
3018 359 145
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1903 1933 1970 2000 3522
Size: 1903 30 37 30 1522
Fragments arranged by size:
1903 1522 37 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 106 1480 1730 1864 3522
Size: 106 1374 250 134 1658
Fragments arranged by size:
1658 1374 250 134 106
BseYI C'CCAG_C
Cuts at: 0 182 2223 3522
Size: 182 2041 1299
Fragments arranged by size:
2041 1299 182
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 100 729 2849 3522
Size: 100 629 2120 673
Fragments arranged by size:
2120 673 629 100
BsiEI CG_ry'CG
Cuts at: 0 72 1206 3522
Size: 72 1134 2316
Fragments arranged by size:
2316 1134 72
BsiHKAI G_wGCw'C
Cuts at: 0 106 166 1094 1549 2442 3522
Size: 106 60 928 455 893 1080
Fragments arranged by size:
1080 928 893 455 106 60
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1872 3522
Size: 1872 1650
Bsp1286I G_dGCh'C
Cuts at: 0 106 166 421 1094 1187 1344 1549 1749
Size: 106 60 255 673 93 157 205 200
Cuts at: 1749 2442 3522
Size: 693 1080
Fragments arranged by size:
1080 693 673 255 205 200 157 106
93 60
BspHI T'CATG_A
Cuts at: 0 1275 1437 3522
Size: 1275 162 2085
Fragments arranged by size:
2085 1275 162
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 256 3522
Size: 256 3266
BsrBI CCG'CTC
Cuts at: 0 1462 3522
Size: 1462 2060
BsrFI r'CCGG_y
Cuts at: 0 127 152 504 3522
Size: 127 25 352 3018
Fragments arranged by size:
3018 352 127 25
BssSI C'ACGA_G
Cuts at: 0 437 3522
Size: 437 3085
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1078 2181 3522
Size: 1078 1103 1341
Fragments arranged by size:
1341 1103 1078
BstEII G'GTnAC_C
Cuts at: 0 141 3522
Size: 141 3381
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1587 2007 3522
Size: 1587 420 1515
Fragments arranged by size:
1587 1515 420
BstYI r'GATC_y
Cuts at: 0 9 26 908 1152 2802 3522
Size: 9 17 882 244 1650 720
Fragments arranged by size:
1650 882 720 244 17 9
Bsu36I CC'TnA_GG
Cuts at: 0 2923 3266 3398 3522
Size: 2923 343 132 124
Fragments arranged by size:
2923 343 132 124
BtgI C'CryG_G
Cuts at: 0 1586 1638 3522
Size: 1586 52 1884
Fragments arranged by size:
1884 1586 52
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 3449 3522
Size: 3449 73
BtsI GCAGTG_nn'
Cuts at: 0 410 1425 2306 2837 3053 3434 3522
Size: 410 1015 881 531 216 381 88
Fragments arranged by size:
1015 881 531 410 381 216 88
DraIII CAC_nnn'GTG
Cuts at: 0 1499 3522
Size: 1499 2023
DrdI GACnn_nn'nnGTC
Cuts at: 0 1809 3522
Size: 1809 1713
EaeI y'GGCC_r
Cuts at: 0 69 349 3522
Size: 69 280 3173
Fragments arranged by size:
3173 280 69
EagI C'GGCC_G
Cuts at: 0 69 3522
Size: 69 3453
EarI CTCTTCn'nnn_
Cuts at: 0 614 2126 2876 3317 3522
Size: 614 1512 750 441 205
Fragments arranged by size:
1512 750 614 441 205
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 22 3522
Size: 22 3500
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 40 353 377 698 1385 2084 2125 2666
Size: 40 313 24 321 687 699 41 541
Cuts at: 2666 2945 3132 3353 3522
Size: 279 187 221 169
Fragments arranged by size:
699 687 541 321 313 279 221 187
169 41 40 24
EcoO109I rG'GnC_Cy
Cuts at: 0 1183 1184 1453 1745 3522
Size: 1183 1 269 292 1777
Fragments arranged by size:
1777 1183 292 269 1
EcoRI G'AATT_C
Cuts at: 0 1038 2429 2465 3522
Size: 1038 1391 36 1057
Fragments arranged by size:
1391 1057 1038 36
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2155 3522
Size: 2155 1367
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1051 1083 1768 1800 2787 2819 3294 3326
Size: 1051 32 685 32 987 32 475 32
Cuts at: 3326 3522
Size: 196
Fragments arranged by size:
1051 987 685 475 196 32 32 32
32
HincII GTy'rAC
Cuts at: 0 2612 3357 3522
Size: 2612 745 165
Fragments arranged by size:
2612 745 165
HindIII A'AGCT_T
Cuts at: 0 371 1942 2177 3522
Size: 371 1571 235 1345
Fragments arranged by size:
1571 1345 371 235
HpaI GTT'AAC
Cuts at: 0 2612 3522
Size: 2612 910
MfeI C'AATT_G
Cuts at: 0 285 3522
Size: 285 3237
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1220 2691 3522
Size: 1220 1471 831
Fragments arranged by size:
1471 1220 831
MslI CAynn'nnrTG
Cuts at: 0 1766 2402 3517 3522
Size: 1766 636 1115 5
Fragments arranged by size:
1766 1115 636 5
MspA1I CmG'CkG
Cuts at: 0 1795 2019 2391 3522
Size: 1795 224 372 1131
Fragments arranged by size:
1795 1131 372 224
NcoI C'CATG_G
Cuts at: 0 1586 3522
Size: 1586 1936
NdeI CA'TA_TG
Cuts at: 0 3183 3522
Size: 3183 339
NsiI A_TGCA'T
Cuts at: 0 2213 3522
Size: 2213 1309
NspI r_CATG'y
Cuts at: 0 729 1076 1765 2125 2215 3522
Size: 729 347 689 360 90 1307
Fragments arranged by size:
1307 729 689 360 347 90
PflMI CCAn_nnn'nTGG
Cuts at: 0 1350 3141 3522
Size: 1350 1791 381
Fragments arranged by size:
1791 1350 381
PfoI T'CCnGG_A
Cuts at: 0 43 1907 3522
Size: 43 1864 1615
Fragments arranged by size:
1864 1615 43
PpuMI rG'GwC_Cy
Cuts at: 0 1453 3522
Size: 1453 2069
PsiI TTA'TAA
Cuts at: 0 530 1535 3522
Size: 530 1005 1987
Fragments arranged by size:
1987 1005 530
PspOMI G'GGCC_C
Cuts at: 0 1183 1745 3522
Size: 1183 562 1777
Fragments arranged by size:
1777 1183 562
PstI C_TGCA'G
Cuts at: 0 1420 3522
Size: 1420 2102
PvuI CG_AT'CG
Cuts at: 0 1206 3522
Size: 1206 2316
PvuII CAG'CTG
Cuts at: 0 1795 2019 2391 3522
Size: 1795 224 372 1131
Fragments arranged by size:
1795 1131 372 224
SapI GCTCTTCn'nnn_
Cuts at: 0 2876 3317 3522
Size: 2876 441 205
Fragments arranged by size:
2876 441 205
ScaI AGT'ACT
Cuts at: 0 543 1566 2859 3522
Size: 543 1023 1293 663
Fragments arranged by size:
1293 1023 663 543
SfcI C'TryA_G
Cuts at: 0 422 1095 1416 2516 3522
Size: 422 673 321 1100 1006
Fragments arranged by size:
1100 1006 673 422 321
SgrAI Cr'CCGG_yG
Cuts at: 0 127 152 3522
Size: 127 25 3370
Fragments arranged by size:
3370 127 25
SmlI C'TyrA_G
Cuts at: 0 1110 1945 2170 2300 2778 3287 3522
Size: 1110 835 225 130 478 509 235
Fragments arranged by size:
1110 835 509 478 235 225 130
SphI G_CATG'C
Cuts at: 0 1076 2125 2215 3522
Size: 1076 1049 90 1307
Fragments arranged by size:
1307 1076 1049 90
SspI AAT'ATT
Cuts at: 0 1596 3522
Size: 1596 1926
StuI AGG'CCT
Cuts at: 0 1404 3522
Size: 1404 2118
StyI C'CwwG_G
Cuts at: 0 1586 1833 2400 2866 3522
Size: 1586 247 567 466 656
Fragments arranged by size:
1586 656 567 466 247
TatI w'GTAC_w
Cuts at: 0 541 804 1564 2561 2857 3522
Size: 541 263 760 997 296 665
Fragments arranged by size:
997 760 665 541 296 263
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1970 2002 3522
Size: 1970 32 1520
Fragments arranged by size:
1970 1520 32
XbaI T'CTAG_A
Cuts at: 0 3406 3522
Size: 3406 116
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2238 2313 2707 3522
Size: 2238 75 394 815
Fragments arranged by size:
2238 815 394 75
XhoI C'TCGA_G
Cuts at: 0 2300 3522
Size: 2300 1222
XmnI GAAnn'nnTTC
Cuts at: 0 1224 3110 3522
Size: 1224 1886 412
Fragments arranged by size:
1886 1224 412
Enzymes that do cut:
AcuI AflIII AgeI AhdI AleI AlfI AlwNI ApaI
ApaLI ApoI AseI AvaI BanII BbsI BciVI BfrBI
BglI BglII Bme1580I BplI BpmI Bpu10I BpuEI BsaAI
BsaBI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmBI Bsp1286I BspHI BspMI BsrBI BsrFI BssSI BstAPI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
FalI Hin4I HincII HindIII HpaI MfeI MmeI MslI
MspA1I NcoI NdeI NsiI NspI PflMI PfoI PpuMI
PsiI PspOMI PstI PvuI PvuII SapI ScaI SfcI
SgrAI SmlI SphI SspI StuI StyI TatI TstI
XbaI XcmI XhoI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AloI
AscI AsiSI AvrII BaeI BamHI BanI BbeI BbvCI
BcgI BclI BlpI BmgBI BmrI BmtI BsaI BsaHI
BsiWI BsmI BspEI BsrDI BsrGI BssHII BstBI BstZ17I
ClaI CspCI DraI EcoICRI EcoNI EcoRV FseI FspI
FspAI HaeII KasI KpnI MluI MscI NaeI NarI
NgoMIV NheI NotI NruI PacI PasI PciI PmeI
PmlI PpiI PshAI PspXI PsrI RsrII SacI SacII
SalI SanDI SbfI SexAI SfiI SfoI SgfI SmaI
SnaBI SpeI SrfI SwaI TaqII Tth111I XmaI ZraI