Restriction map for
pF1KSDA1688
(Linear) (Six-base) MAPSORT of: KIAA1688.seq Check: 6272 from: 1 to: 3249
KIAA1688.orf
With 182 enzymes: SgfI *
October 24, 2006 15:17 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2826 3249
Size: 2826 423
AccI GT'mk_AC
Cuts at: 0 41 1196 3249
Size: 41 1155 2053
Fragments arranged by size:
2053 1155 41
Acc65I G'GTAC_C
Cuts at: 0 2906 3249
Size: 2906 343
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 346 847 1791 2620 2775 2848 2917 3249
Size: 346 501 944 829 155 73 69 332
Fragments arranged by size:
944 829 501 346 332 155 73 69
AfeI AGC'GCT
Cuts at: 0 1141 2704 3249
Size: 1141 1563 545
Fragments arranged by size:
1563 1141 545
AflIII A'CryG_T
Cuts at: 0 227 3249
Size: 227 3022
AgeI A'CCGG_T
Cuts at: 0 142 3249
Size: 142 3107
AhdI GACnn_n'nnGTC
Cuts at: 0 1443 3249
Size: 1443 1806
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2689 3249
Size: 2689 560
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 325 357 2077 2109 3249
Size: 325 32 1720 32 1140
Fragments arranged by size:
1720 1140 325 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 324 1110 1298 1320 1563 1847 1890 2337
Size: 324 786 188 22 243 284 43 447
Cuts at: 2337 3219 3249
Size: 882 30
Fragments arranged by size:
882 786 447 324 284 243 188 43
30 22
ApaI G_GGCC'C
Cuts at: 0 1090 1537 3249
Size: 1090 447 1712
Fragments arranged by size:
1712 1090 447
ApaLI G'TGCA_C
Cuts at: 0 2998 3249
Size: 2998 251
AvaI C'yCGr_G
Cuts at: 0 370 424 618 1135 1464 1537 1786 2710
Size: 370 54 194 517 329 73 249 924
Cuts at: 2710 2977 3249
Size: 267 272
Fragments arranged by size:
924 517 370 329 272 267 249 194
73 54
BanI G'GyrC_C
Cuts at: 0 275 622 787 1765 1858 2853 2906 3123
Size: 275 347 165 978 93 995 53 217
Cuts at: 3123 3249
Size: 126
Fragments arranged by size:
995 978 347 275 217 165 126 93
53
BanII G_rGCy'C
Cuts at: 0 425 431 665 842 947 983 1090 1216
Size: 425 6 234 177 105 36 107 126
Cuts at: 1216 1537 1655 1940 2237 2351 2420 2927 3249
Size: 321 118 285 297 114 69 507 322
Fragments arranged by size:
507 425 322 321 297 285 234 177
126 118 114 107 105 69 36 6
BbeI G_GCGC'C
Cuts at: 0 626 1769 3127 3249
Size: 626 1143 1358 122
Fragments arranged by size:
1358 1143 626 122
BbsI GAAGACnn'nnnn_
Cuts at: 0 2028 2876 3051 3249
Size: 2028 848 175 198
Fragments arranged by size:
2028 848 198 175
BbvCI CC'TCA_GC
Cuts at: 0 1306 3249
Size: 1306 1943
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2803 2837 3249
Size: 2803 34 412
Fragments arranged by size:
2803 412 34
BglI GCCn_nnn'nGGC
Cuts at: 0 398 778 1633 1684 2012 3249
Size: 398 380 855 51 328 1237
Fragments arranged by size:
1237 855 398 380 328 51
Bme1580I G_kGCm'C
Cuts at: 0 1090 1471 1537 1861 1983 2858 3002 3249
Size: 1090 381 66 324 122 875 144 247
Fragments arranged by size:
1090 875 381 324 247 144 122 66
BmgBI CAC'GTC
Cuts at: 0 230 993 2880 3249
Size: 230 763 1887 369
Fragments arranged by size:
1887 763 369 230
BmrI ACTGGGnnnn_n'
Cuts at: 0 943 1977 2097 3249
Size: 943 1034 120 1152
Fragments arranged by size:
1152 1034 943 120
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 439 1054 1174 1200 1869 1915 2010 2184
Size: 439 615 120 26 669 46 95 174
Cuts at: 2184 2323 2343 2413 2938 3249
Size: 139 20 70 525 311
Fragments arranged by size:
669 615 525 439 311 174 139 120
95 70 46 26 20
Bpu10I CC'TnA_GC
Cuts at: 0 1147 1306 1361 3249
Size: 1147 159 55 1888
Fragments arranged by size:
1888 1147 159 55
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 556 3249
Size: 556 2693
BsaAI yAC'GTr
Cuts at: 0 1182 1200 2214 2295 2537 3249
Size: 1182 18 1014 81 242 712
Fragments arranged by size:
1182 1014 712 242 81 18
BsaHI Gr'CG_yC
Cuts at: 0 173 623 1325 1766 3124 3249
Size: 173 450 702 441 1358 125
Fragments arranged by size:
1358 702 450 441 173 125
BsaWI w'CCGG_w
Cuts at: 0 142 1333 3249
Size: 142 1191 1916
Fragments arranged by size:
1916 1191 142
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 583 613 2077 2107 3249
Size: 583 30 1464 30 1142
Fragments arranged by size:
1464 1142 583 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 402 591 822 894 955 1731 1866 2055
Size: 402 189 231 72 61 776 135 189
Cuts at: 2055 2632 2764 2935 3249
Size: 577 132 171 314
Fragments arranged by size:
776 577 402 314 231 189 189 171
135 132 72 61
BseYI C'CCAG_C
Cuts at: 0 664 754 869 1034 1291 1724 1733 1829
Size: 664 90 115 165 257 433 9 96
Cuts at: 1829 2020 2411 3249
Size: 191 391 838
Fragments arranged by size:
838 664 433 391 257 191 165 115
96 90 9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 45 1933 2275 2350 2752 3085 3249
Size: 45 1888 342 75 402 333 164
Fragments arranged by size:
1888 402 342 333 164 75 45
BsiEI CG_ry'CG
Cuts at: 0 170 283 1042 1063 1649 2208 3249
Size: 170 113 759 21 586 559 1041
Fragments arranged by size:
1041 759 586 559 170 113 21
BsiHKAI G_wGCw'C
Cuts at: 0 842 1118 2237 2351 2420 3002 3209 3249
Size: 842 276 1119 114 69 582 207 40
Fragments arranged by size:
1119 842 582 276 207 114 69 40
BsmI GAATG_Cn'
Cuts at: 0 2826 3249
Size: 2826 423
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1911 2065 3249
Size: 1911 154 1184
Fragments arranged by size:
1911 1184 154
Bsp1286I G_dGCh'C
Cuts at: 0 425 431 665 842 947 983 1090 1118
Size: 425 6 234 177 105 36 107 28
Cuts at: 1118 1216 1471 1537 1655 1861 1940 1983 2237
Size: 98 255 66 118 206 79 43 254
Cuts at: 2237 2351 2420 2858 2927 3002 3209 3249
Size: 114 69 438 69 75 207 40
Fragments arranged by size:
438 425 255 254 234 207 206 177
118 114 107 105 98 79 75 69
69 66 43 40 36 28 6
BspEI T'CCGG_A
Cuts at: 0 1333 3249
Size: 1333 1916
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 53 1553 2826 3045 3249
Size: 53 1500 1273 219 204
Fragments arranged by size:
1500 1273 219 204 53
BsrBI CCG'CTC
Cuts at: 0 722 933 1615 3249
Size: 722 211 682 1634
Fragments arranged by size:
1634 722 682 211
BsrDI GCAATG_nn'
Cuts at: 0 693 3249
Size: 693 2556
BsrFI r'CCGG_y
Cuts at: 0 142 165 169 278 1024 1168 1234 1791
Size: 142 23 4 109 746 144 66 557
Cuts at: 1791 1997 2207 2267 2366 2489 3069 3249
Size: 206 210 60 99 123 580 180
Fragments arranged by size:
746 580 557 210 206 180 144 142
123 109 99 66 60 23 4
BsrGI T'GTAC_A
Cuts at: 0 2255 3249
Size: 2255 994
BssHII G'CGCG_C
Cuts at: 0 348 1230 3249
Size: 348 882 2019
Fragments arranged by size:
2019 882 348
BssSI C'ACGA_G
Cuts at: 0 2938 3249
Size: 2938 311
BstAPI GCAn_nnn'nTGC
Cuts at: 0 453 1113 2337 3006 3249
Size: 453 660 1224 669 243
Fragments arranged by size:
1224 669 660 453 243
BstEII G'GTnAC_C
Cuts at: 0 138 2263 2770 3084 3249
Size: 138 2125 507 314 165
Fragments arranged by size:
2125 507 314 165 138
BstXI CCAn_nnnn'nTGG
Cuts at: 0 688 2157 2433 2865 3120 3234 3249
Size: 688 1469 276 432 255 114 15
Fragments arranged by size:
1469 688 432 276 255 114 15
BstYI r'GATC_y
Cuts at: 0 1886 2621 3249
Size: 1886 735 628
Fragments arranged by size:
1886 735 628
BtgI C'CryG_G
Cuts at: 0 476 986 1240 1349 2406 2993 3113 3249
Size: 476 510 254 109 1057 587 120 136
Fragments arranged by size:
1057 587 510 476 254 136 120 109
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2130 2943 3249
Size: 2130 813 306
Fragments arranged by size:
2130 813 306
BtsI GCAGTG_nn'
Cuts at: 0 1774 2287 2671 2959 3249
Size: 1774 513 384 288 290
Fragments arranged by size:
1774 513 384 290 288
DraIII CAC_nnn'GTG
Cuts at: 0 272 3249
Size: 272 2977
DrdI GACnn_nn'nnGTC
Cuts at: 0 2878 3160 3249
Size: 2878 282 89
Fragments arranged by size:
2878 282 89
EaeI y'GGCC_r
Cuts at: 0 167 280 308 576 1060 1565 1646 2309
Size: 167 113 28 268 484 505 81 663
Cuts at: 2309 2423 2990 3071 3110 3249
Size: 114 567 81 39 139
Fragments arranged by size:
663 567 505 484 268 167 139 114
113 81 81 39 28
EagI C'GGCC_G
Cuts at: 0 167 280 1060 1646 3249
Size: 167 113 780 586 1603
Fragments arranged by size:
1603 780 586 167 113
EarI CTCTTCn'nnn_
Cuts at: 0 519 823 1477 1894 2131 2242 3249
Size: 519 304 654 417 237 111 1007
Fragments arranged by size:
1007 654 519 417 304 237 111
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 373 2148 2377 3249
Size: 373 1775 229 872
Fragments arranged by size:
1775 872 373 229
EcoICRI GAG'CTC
Cuts at: 0 840 2235 2349 2418 3249
Size: 840 1395 114 69 831
Fragments arranged by size:
1395 840 831 114 69
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 346 439 847 1054 1174 1200 1791 1869
Size: 346 93 408 207 120 26 591 78
Cuts at: 1869 1915 2010 2184 2323 2343 2413 2620 2775
Size: 46 95 174 139 20 70 207 155
Cuts at: 2775 2848 2917 2938 3249
Size: 73 69 21 311
Fragments arranged by size:
591 408 346 311 207 207 174 155
139 120 95 93 78 73 70 69
46 26 21 20
EcoNI CCTnn'n_nnAGG
Cuts at: 0 538 1567 1663 3249
Size: 538 1029 96 1586
Fragments arranged by size:
1586 1029 538 96
EcoO109I rG'GnC_Cy
Cuts at: 0 159 596 963 1086 1087 1357 1533 1534
Size: 159 437 367 123 1 270 176 1
Cuts at: 1534 1542 1965 2121 2334 2796 3249
Size: 8 423 156 213 462 453
Fragments arranged by size:
462 453 437 423 367 270 213 176
159 156 123 8 1 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1893 3249
Size: 1893 1356
FspI TGC'GCA
Cuts at: 0 2056 3249
Size: 2056 1193
HaeII r_GCGC'y
Cuts at: 0 626 1143 1320 1520 1769 1825 2706 2718
Size: 626 517 177 200 249 56 881 12
Cuts at: 2718 2764 3127 3249
Size: 46 363 122
Fragments arranged by size:
881 626 517 363 249 200 177 122
56 46 12
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1402 1429 1434 1461 3249
Size: 1402 27 5 27 1788
Fragments arranged by size:
1788 1402 27 27 5
HincII GTy'rAC
Cuts at: 0 42 3249
Size: 42 3207
KasI G'GCGC_C
Cuts at: 0 622 1765 3123 3249
Size: 622 1143 1358 126
Fragments arranged by size:
1358 1143 622 126
KpnI G_GTAC'C
Cuts at: 0 2910 3249
Size: 2910 339
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 62 3173 3249
Size: 62 3111 76
Fragments arranged by size:
3111 76 62
MscI TGG'CCA
Cuts at: 0 310 2311 2425 3112 3249
Size: 310 2001 114 687 137
Fragments arranged by size:
2001 687 310 137 114
MslI CAynn'nnrTG
Cuts at: 0 991 2194 2689 3091 3118 3249
Size: 991 1203 495 402 27 131
Fragments arranged by size:
1203 991 495 402 131 27
MspA1I CmG'CkG
Cuts at: 0 306 478 506 861 1038 1110 1191 1242
Size: 306 172 28 355 177 72 81 51
Cuts at: 1242 1392 1724 2012 2030 2361 2408 2721 3249
Size: 150 332 288 18 331 47 313 528
Fragments arranged by size:
528 355 332 331 313 306 288 177
172 150 81 72 51 47 28 18
NaeI GCC'GGC
Cuts at: 0 167 171 1026 1170 1236 1999 2368 3071
Size: 167 4 855 144 66 763 369 703
Cuts at: 3071 3249
Size: 178
Fragments arranged by size:
855 763 703 369 178 167 144 66
4
NarI GG'CG_CC
Cuts at: 0 623 1766 3124 3249
Size: 623 1143 1358 125
Fragments arranged by size:
1358 1143 623 125
NcoI C'CATG_G
Cuts at: 0 986 1349 3113 3249
Size: 986 363 1764 136
Fragments arranged by size:
1764 986 363 136
NgoMIV G'CCGG_C
Cuts at: 0 165 169 1024 1168 1234 1997 2366 3069
Size: 165 4 855 144 66 763 369 703
Cuts at: 3069 3249
Size: 180
Fragments arranged by size:
855 763 703 369 180 165 144 66
4
NspI r_CATG'y
Cuts at: 0 126 639 2157 2632 2694 3249
Size: 126 513 1518 475 62 555
Fragments arranged by size:
1518 555 513 475 126 62
PasI CC'CwG_GG
Cuts at: 0 681 844 2726 2800 3249
Size: 681 163 1882 74 449
Fragments arranged by size:
1882 681 449 163 74
PflMI CCAn_nnn'nTGG
Cuts at: 0 1110 1320 1421 2321 3219 3249
Size: 1110 210 101 900 898 30
Fragments arranged by size:
1110 900 898 210 101 30
PmlI CAC'GTG
Cuts at: 0 2214 2295 3249
Size: 2214 81 954
Fragments arranged by size:
2214 954 81
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 917 949 2077 2109 3249
Size: 917 32 1128 32 1140
Fragments arranged by size:
1140 1128 917 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 159 596 1357 1542 1965 2796 3249
Size: 159 437 761 185 423 831 453
Fragments arranged by size:
831 761 453 437 423 185 159
PspOMI G'GGCC_C
Cuts at: 0 1086 1533 3249
Size: 1086 447 1716
Fragments arranged by size:
1716 1086 447
PstI C_TGCA'G
Cuts at: 0 320 764 2678 2837 3056 3249
Size: 320 444 1914 159 219 193
Fragments arranged by size:
1914 444 320 219 193 159
PvuII CAG'CTG
Cuts at: 0 1110 1191 1724 2030 2361 2721 3249
Size: 1110 81 533 306 331 360 528
Fragments arranged by size:
1110 533 528 360 331 306 81
SacI G_AGCT'C
Cuts at: 0 842 2237 2351 2420 3249
Size: 842 1395 114 69 829
Fragments arranged by size:
1395 842 829 114 69
SacII CC_GC'GG
Cuts at: 0 479 1243 2409 3249
Size: 479 764 1166 840
Fragments arranged by size:
1166 840 764 479
SalI G'TCGA_C
Cuts at: 0 40 3249
Size: 40 3209
SanDI GG'GwC_CC
Cuts at: 0 159 2796 3249
Size: 159 2637 453
Fragments arranged by size:
2637 453 159
SapI GCTCTTCn'nnn_
Cuts at: 0 519 1477 1894 2242 3249
Size: 519 958 417 348 1007
Fragments arranged by size:
1007 958 519 417 348
SbfI CC_TGCA'GG
Cuts at: 0 3056 3249
Size: 3056 193
ScaI AGT'ACT
Cuts at: 0 2548 3249
Size: 2548 701
SexAI A'CCwGG_T
Cuts at: 0 134 3249
Size: 134 3115
SfcI C'TryA_G
Cuts at: 0 316 591 760 1341 2563 2674 2833 3052
Size: 316 275 169 581 1222 111 159 219
Cuts at: 3052 3249
Size: 197
Fragments arranged by size:
1222 581 316 275 219 197 169 159
111
SfoI GGC'GCC
Cuts at: 0 624 1767 3125 3249
Size: 624 1143 1358 124
Fragments arranged by size:
1358 1143 624 124
SgrAI Cr'CCGG_yG
Cuts at: 0 142 3249
Size: 142 3107
SmaI CCC'GGG
Cuts at: 0 372 1466 1539 1788 3249
Size: 372 1094 73 249 1461
Fragments arranged by size:
1461 1094 372 249 73
SmlI C'TyrA_G
Cuts at: 0 535 3249
Size: 535 2714
SphI G_CATG'C
Cuts at: 0 639 2632 2694 3249
Size: 639 1993 62 555
Fragments arranged by size:
1993 639 555 62
SrfI GCCC'GGGC
Cuts at: 0 1788 3249
Size: 1788 1461
StuI AGG'CCT
Cuts at: 0 2227 2864 3249
Size: 2227 637 385
Fragments arranged by size:
2227 637 385
StyI C'CwwG_G
Cuts at: 0 986 1349 2276 2315 3113 3249
Size: 986 363 927 39 798 136
Fragments arranged by size:
986 927 798 363 136 39
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 78 768 2251 2718 3249
Size: 78 690 1483 467 531
Fragments arranged by size:
1483 690 531 467 78
TatI w'GTAC_w
Cuts at: 0 1157 2255 2546 3249
Size: 1157 1098 291 703
Fragments arranged by size:
1157 1098 703 291
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 7 39 701 733 3249
Size: 7 32 662 32 2516
Fragments arranged by size:
2516 662 32 32 7
Tth111I GACn'n_nGTC
Cuts at: 0 2503 3249
Size: 2503 746
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 414 3249
Size: 414 2835
XmaI C'CCGG_G
Cuts at: 0 370 1464 1537 1786 3249
Size: 370 1094 73 249 1463
Fragments arranged by size:
1463 1094 370 249 73
Enzymes that do cut:
AarI AccI Acc65I AcuI AfeI AflIII AgeI AhdI
AlfI AloI AlwNI ApaI ApaLI AvaI BanI BanII
BbeI BbsI BbvCI BcgI BglI Bme1580I BmgBI BmrI
BpmI Bpu10I BpuEI BsaAI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstEII BstXI BstYI BtgI BtgZI BtsI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
FalI FspI HaeII Hin4I HincII KasI KpnI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PasI PflMI PmlI PpiI PpuMI PspOMI PstI PvuII
SacI SacII SalI SanDI SapI SbfI ScaI SexAI
SfcI SfoI SgrAI SmaI SmlI SphI SrfI StuI
StyI TaqII TatI TstI Tth111I XcmI XmaI
Enzymes that do not cut:
AatII AclI AflII AleI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BciVI BclI BfrBI BglII BlpI
BmtI BplI BsaI BsaBI BsiWI BspHI BstBI BstZ17I
Bsu36I ClaI CspCI DraI EcoRI EcoRV FseI FspAI
HindIII HpaI MfeI MluI NdeI NheI NotI NruI
NsiI PacI PciI PfoI PmeI PshAI PsiI PspXI
PsrI PvuI RsrII SfiI SgfI SnaBI SpeI SspI
SwaI XbaI XhoI XmnI ZraI