Restriction map for
pF1KA1689
(Linear) (Six-base) MAPSORT of: KIAA1689.seq Check: 584 from: 1 to: 7872
KIAA1689.orf
With 182 enzymes: SgfI *
July 18, 2007 13:36 ..
AccI GT'mk_AC
Cuts at: 0 1370 4775 6745 7872
Size: 1370 3405 1970 1127
Fragments arranged by size:
3405 1970 1370 1127
Acc65I G'GTAC_C
Cuts at: 0 2580 5929 7872
Size: 2580 3349 1943
Fragments arranged by size:
3349 2580 1943
AclI AA'CG_TT
Cuts at: 0 7155 7872
Size: 7155 717
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 320 707 1096 1148 1661 1676 1928 3905
Size: 320 387 389 52 513 15 252 1977
Cuts at: 3905 4014 4125 4711 5333 5873 6524 6998 7872
Size: 109 111 586 622 540 651 474 874
Fragments arranged by size:
1977 874 651 622 586 540 513 474
389 387 320 252 111 109 52 15
AflIII A'CryG_T
Cuts at: 0 6599 7872
Size: 6599 1273
AleI CACnn'nnGTG
Cuts at: 0 154 5842 7198 7872
Size: 154 5688 1356 674
Fragments arranged by size:
5688 1356 674 154
AlwNI CAG_nnn'CTG
Cuts at: 0 450 2032 7825 7872
Size: 450 1582 5793 47
Fragments arranged by size:
5793 1582 450 47
ApaI G_GGCC'C
Cuts at: 0 6641 7800 7872
Size: 6641 1159 72
Fragments arranged by size:
6641 1159 72
ApaLI G'TGCA_C
Cuts at: 0 7201 7872
Size: 7201 671
ApoI r'AATT_y
Cuts at: 0 1018 1271 1926 2168 2477 2946 3698 3864
Size: 1018 253 655 242 309 469 752 166
Cuts at: 3864 4113 4578 4863 5041 5061 5874 6675 6898
Size: 249 465 285 178 20 813 801 223
Cuts at: 6898 7872
Size: 974
Fragments arranged by size:
1018 974 813 801 752 655 469 465
309 285 253 249 242 223 178 166
20
AseI AT'TA_AT
Cuts at: 0 362 2594 4238 7872
Size: 362 2232 1644 3634
Fragments arranged by size:
3634 2232 1644 362
AvaI C'yCGr_G
Cuts at: 0 737 7872
Size: 737 7135
AvrII C'CTAG_G
Cuts at: 0 202 7872
Size: 202 7670
BanI G'GyrC_C
Cuts at: 0 52 2580 5929 7872
Size: 52 2528 3349 1943
Fragments arranged by size:
3349 2528 1943 52
BanII G_rGCy'C
Cuts at: 0 64 149 188 2348 6586 6641 6692 7800
Size: 64 85 39 2160 4238 55 51 1108
Cuts at: 7800 7872
Size: 72
Fragments arranged by size:
4238 2160 1108 85 72 64 55 51
39
BbeI G_GCGC'C
Cuts at: 0 56 7872
Size: 56 7816
BbsI GAAGACnn'nnnn_
Cuts at: 0 1667 2746 2911 3076 3232 3394 3559 6331
Size: 1667 1079 165 165 156 162 165 2772
Cuts at: 6331 7466 7512 7872
Size: 1135 46 360
Fragments arranged by size:
2772 1667 1135 1079 360 165 165 165
162 156 46
BbvCI CC'TCA_GC
Cuts at: 0 1748 3578 7872
Size: 1748 1830 4294
Fragments arranged by size:
4294 1830 1748
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1081 1115 7872
Size: 1081 34 6757
Fragments arranged by size:
6757 1081 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 3141 7872
Size: 3141 4731
BclI T'GATC_A
Cuts at: 0 1806 7752 7872
Size: 1806 5946 120
Fragments arranged by size:
5946 1806 120
BfrBI ATG'CAT
Cuts at: 0 4089 5807 7360 7364 7872
Size: 4089 1718 1553 4 508
Fragments arranged by size:
4089 1718 1553 508 4
BglII A'GATC_T
Cuts at: 0 256 1288 3429 3890 5725 5979 7348 7630
Size: 256 1032 2141 461 1835 254 1369 282
Cuts at: 7630 7872
Size: 242
Fragments arranged by size:
2141 1835 1369 1032 461 282 256 254
242
BlpI GC'TnA_GC
Cuts at: 0 5054 5201 6302 7731 7872
Size: 5054 147 1101 1429 141
Fragments arranged by size:
5054 1429 1101 147 141
Bme1580I G_kGCm'C
Cuts at: 0 15 161 6641 7205 7788 7800 7872
Size: 15 146 6480 564 583 12 72
Fragments arranged by size:
6480 583 564 146 72 15 12
BmrI ACTGGGnnnn_n'
Cuts at: 0 1432 7872
Size: 1432 6440
BmtI G_CTAG'C
Cuts at: 0 3724 7872
Size: 3724 4148
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 3558 7221 7872
Size: 3558 3663 651
Fragments arranged by size:
3663 3558 651
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 626 858 4193 5239 5684 6599 7280 7872
Size: 626 232 3335 1046 445 915 681 592
Fragments arranged by size:
3335 1046 915 681 626 592 445 232
Bpu10I CC'TnA_GC
Cuts at: 0 19 1514 1748 3578 7872
Size: 19 1495 234 1830 4294
Fragments arranged by size:
4294 1830 1495 234 19
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 3528 3969 4244 6340 6779 6815 7787 7872
Size: 3528 441 275 2096 439 36 972 85
Fragments arranged by size:
3528 2096 972 441 439 275 85 36
BsaI GGTCTCn'nnnn_
Cuts at: 0 3967 4725 4731 5725 7872
Size: 3967 758 6 994 2147
Fragments arranged by size:
3967 2147 994 758 6
BsaAI yAC'GTr
Cuts at: 0 1962 7872
Size: 1962 5910
BsaBI GATnn'nnATC
Cuts at: 0 587 6353 7347 7521 7872
Size: 587 5766 994 174 351
Fragments arranged by size:
5766 994 587 351 174
BsaHI Gr'CG_yC
Cuts at: 0 53 2743 7872
Size: 53 2690 5129
Fragments arranged by size:
5129 2690 53
BsaWI w'CCGG_w
Cuts at: 0 3361 7872
Size: 3361 4511
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 4591 4621 5969 5999 6132 6162 6704 6734
Size: 4591 30 1348 30 133 30 542 30
Cuts at: 6734 7872
Size: 1138
Fragments arranged by size:
4591 1348 1138 542 133 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 2234 2848 3013 3169 3331 3496 3658 3985
Size: 2234 614 165 156 162 165 162 327
Cuts at: 3985 4317 4540 4612 7872
Size: 332 223 72 3260
Fragments arranged by size:
3260 2234 614 332 327 223 165 165
162 162 156 72
BseYI C'CCAG_C
Cuts at: 0 80 1876 5177 6879 7872
Size: 80 1796 3301 1702 993
Fragments arranged by size:
3301 1796 1702 993 80
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1542 2353 6627 6899 7872
Size: 1542 811 4274 272 973
Fragments arranged by size:
4274 1542 973 811 272
BsiHKAI G_wGCw'C
Cuts at: 0 5076 6586 7205 7872
Size: 5076 1510 619 667
Fragments arranged by size:
5076 1510 667 619
BsmI GAATG_Cn'
Cuts at: 0 1063 1537 6240 7872
Size: 1063 474 4703 1632
Fragments arranged by size:
4703 1632 1063 474
BsmBI CGTCTCn'nnnn_
Cuts at: 0 3175 7872
Size: 3175 4697
Bsp1286I G_dGCh'C
Cuts at: 0 15 64 149 161 188 2348 5076 6586
Size: 15 49 85 12 27 2160 2728 1510
Cuts at: 6586 6641 6692 7205 7788 7800 7872
Size: 55 51 513 583 12 72
Fragments arranged by size:
2728 2160 1510 583 513 85 72 55
51 49 27 15 12 12
BspHI T'CATG_A
Cuts at: 0 4170 6108 7872
Size: 4170 1938 1764
Fragments arranged by size:
4170 1938 1764
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1344 2134 7872
Size: 1344 790 5738
Fragments arranged by size:
5738 1344 790
BsrBI CCG'CTC
Cuts at: 0 181 4016 7872
Size: 181 3835 3856
Fragments arranged by size:
3856 3835 181
BsrDI GCAATG_nn'
Cuts at: 0 2717 4029 7872
Size: 2717 1312 3843
Fragments arranged by size:
3843 2717 1312
BsrGI T'GTAC_A
Cuts at: 0 2103 4546 6510 7872
Size: 2103 2443 1964 1362
Fragments arranged by size:
2443 2103 1964 1362
BssSI C'ACGA_G
Cuts at: 0 3557 7872
Size: 3557 4315
BstBI TT'CG_AA
Cuts at: 0 1709 7872
Size: 1709 6163
BstXI CCAn_nnnn'nTGG
Cuts at: 0 771 948 2691 7872
Size: 771 177 1743 5181
Fragments arranged by size:
5181 1743 771 177
BstYI r'GATC_y
Cuts at: 0 256 1288 3429 3880 3890 5725 5979 7086
Size: 256 1032 2141 451 10 1835 254 1107
Cuts at: 7086 7342 7348 7630 7872
Size: 256 6 282 242
Fragments arranged by size:
2141 1835 1107 1032 451 282 256 256
254 242 10 6
BstZ17I GTA'TAC
Cuts at: 0 6746 7872
Size: 6746 1126
Bsu36I CC'TnA_GG
Cuts at: 0 7275 7391 7872
Size: 7275 116 481
Fragments arranged by size:
7275 481 116
BtgI C'CryG_G
Cuts at: 0 119 140 904 1195 7031 7872
Size: 119 21 764 291 5836 841
Fragments arranged by size:
5836 841 764 291 119 21
BtsI GCAGTG_nn'
Cuts at: 0 4074 6911 7872
Size: 4074 2837 961
Fragments arranged by size:
4074 2837 961
DraI TTT'AAA
Cuts at: 0 1023 1172 2621 7872
Size: 1023 149 1449 5251
Fragments arranged by size:
5251 1449 1023 149
EarI CTCTTCn'nnn_
Cuts at: 0 701 1025 1127 1313 2276 2451 2486 2631
Size: 701 324 102 186 963 175 35 145
Cuts at: 2631 2712 2796 3042 3198 3444 3525 3881 4259
Size: 81 84 246 156 246 81 356 378
Cuts at: 4259 4345 5064 5354 5457 5606 5762 6572 6680
Size: 86 719 290 103 149 156 810 108
Cuts at: 6680 7047 7096 7872
Size: 367 49 776
Fragments arranged by size:
963 810 776 719 701 378 367 356
324 290 246 246 186 175 156 156
149 145 108 103 102 86 84 81
81 49 35
EcoICRI GAG'CTC
Cuts at: 0 6584 7872
Size: 6584 1288
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 320 626 707 858 1096 1148 1661 1676
Size: 320 306 81 151 238 52 513 15
Cuts at: 1676 1928 3905 4014 4125 4193 4711 5239 5333
Size: 252 1977 109 111 68 518 528 94
Cuts at: 5333 5684 5873 6524 6599 6998 7280 7872
Size: 351 189 651 75 399 282 592
Fragments arranged by size:
1977 651 592 528 518 513 399 351
320 306 282 252 238 189 151 111
109 94 81 75 68 52 15
EcoNI CCTnn'n_nnAGG
Cuts at: 0 47 1335 1472 3316 7872
Size: 47 1288 137 1844 4556
Fragments arranged by size:
4556 1844 1288 137 47
EcoO109I rG'GnC_Cy
Cuts at: 0 814 1465 6637 6638 7797 7872
Size: 814 651 5172 1 1159 75
Fragments arranged by size:
5172 1159 814 651 75 1
EcoRI G'AATT_C
Cuts at: 0 1926 7872
Size: 1926 5946
EcoRV GAT'ATC
Cuts at: 0 4490 7845 7872
Size: 4490 3355 27
Fragments arranged by size:
4490 3355 27
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 4361 4866 5221 7872
Size: 4361 505 355 2651
Fragments arranged by size:
4361 2651 505 355
HaeII r_GCGC'y
Cuts at: 0 56 1080 7872
Size: 56 1024 6792
Fragments arranged by size:
6792 1024 56
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1168 1200 1642 1674 2007 2039 3787 3819
Size: 1168 32 442 32 333 32 1748 32
Cuts at: 3819 3865 3897 4191 4223 4378 4410 4591 4623
Size: 46 32 294 32 155 32 181 32
Cuts at: 4623 5629 5661 7078 7110 7872
Size: 1006 32 1417 32 762
Fragments arranged by size:
1748 1417 1168 1006 762 442 333 294
181 155 46 32 32 32 32 32
32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 1779 2083 4968 7872
Size: 1779 304 2885 2904
Fragments arranged by size:
2904 2885 1779 304
HindIII A'AGCT_T
Cuts at: 0 4475 7872
Size: 4475 3397
HpaI GTT'AAC
Cuts at: 0 4968 7872
Size: 4968 2904
KasI G'GCGC_C
Cuts at: 0 52 7872
Size: 52 7820
KpnI G_GTAC'C
Cuts at: 0 2584 5933 7872
Size: 2584 3349 1939
Fragments arranged by size:
3349 2584 1939
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1695 5195 5277 5295 6795 7872
Size: 1695 3500 82 18 1500 1077
Fragments arranged by size:
3500 1695 1500 1077 82 18
MslI CAynn'nnrTG
Cuts at: 0 154 946 2689 3787 4520 5593 5842 7198
Size: 154 792 1743 1098 733 1073 249 1356
Cuts at: 7198 7855 7872
Size: 657 17
Fragments arranged by size:
1743 1356 1098 1073 792 733 657 249
154 17
MspA1I CmG'CkG
Cuts at: 0 142 7872
Size: 142 7730
NarI GG'CG_CC
Cuts at: 0 53 7872
Size: 53 7819
NcoI C'CATG_G
Cuts at: 0 904 7031 7872
Size: 904 6127 841
Fragments arranged by size:
6127 904 841
NheI G'CTAG_C
Cuts at: 0 3720 7872
Size: 3720 4152
NsiI A_TGCA'T
Cuts at: 0 4091 5809 7362 7366 7872
Size: 4091 1718 1553 4 506
Fragments arranged by size:
4091 1718 1553 506 4
NspI r_CATG'y
Cuts at: 0 6509 6603 7364 7872
Size: 6509 94 761 508
Fragments arranged by size:
6509 761 508 94
PasI CC'CwG_GG
Cuts at: 0 2651 7512 7872
Size: 2651 4861 360
Fragments arranged by size:
4861 2651 360
PciI A'CATG_T
Cuts at: 0 6599 7872
Size: 6599 1273
PfoI T'CCnGG_A
Cuts at: 0 1067 7872
Size: 1067 6805
PmlI CAC'GTG
Cuts at: 0 1962 7872
Size: 1962 5910
PpuMI rG'GwC_Cy
Cuts at: 0 1465 7872
Size: 1465 6407
PshAI GACnn'nnGTC
Cuts at: 0 93 643 7872
Size: 93 550 7229
Fragments arranged by size:
7229 550 93
PspOMI G'GGCC_C
Cuts at: 0 6637 7796 7872
Size: 6637 1159 76
Fragments arranged by size:
6637 1159 76
PspXI vC'TCGA_Gb
Cuts at: 0 737 7872
Size: 737 7135
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 5813 5845 7872
Size: 5813 32 2027
Fragments arranged by size:
5813 2027 32
PstI C_TGCA'G
Cuts at: 0 2072 5159 7872
Size: 2072 3087 2713
Fragments arranged by size:
3087 2713 2072
SacI G_AGCT'C
Cuts at: 0 6586 7872
Size: 6586 1286
SacII CC_GC'GG
Cuts at: 0 143 7872
Size: 143 7729
SapI GCTCTTCn'nnn_
Cuts at: 0 2631 5064 5354 5762 6680 7872
Size: 2631 2433 290 408 918 1192
Fragments arranged by size:
2631 2433 1192 918 408 290
ScaI AGT'ACT
Cuts at: 0 213 300 675 4076 7872
Size: 213 87 375 3401 3796
Fragments arranged by size:
3796 3401 375 213 87
SexAI A'CCwGG_T
Cuts at: 0 5925 7872
Size: 5925 1947
SfcI C'TryA_G
Cuts at: 0 791 2009 2068 3011 3494 3656 4097 4361
Size: 791 1218 59 943 483 162 441 264
Cuts at: 4361 5155 5327 7872
Size: 794 172 2545
Fragments arranged by size:
2545 1218 943 794 791 483 441 264
172 162 59
SfoI GGC'GCC
Cuts at: 0 54 7872
Size: 54 7818
SmlI C'TyrA_G
Cuts at: 0 737 3543 3984 4259 6319 6794 6830 7802
Size: 737 2806 441 275 2060 475 36 972
Cuts at: 7802 7872
Size: 70
Fragments arranged by size:
2806 2060 972 737 475 441 275 70
36
SphI G_CATG'C
Cuts at: 0 7364 7872
Size: 7364 508
SspI AAT'ATT
Cuts at: 0 7722 7831 7872
Size: 7722 109 41
Fragments arranged by size:
7722 109 41
StuI AGG'CCT
Cuts at: 0 42 1512 7872
Size: 42 1470 6360
Fragments arranged by size:
6360 1470 42
StyI C'CwwG_G
Cuts at: 0 202 904 2348 2896 2980 3061 3217 3379
Size: 202 702 1444 548 84 81 156 162
Cuts at: 3379 3952 5582 7031 7872
Size: 573 1630 1449 841
Fragments arranged by size:
1630 1449 1444 841 702 573 548 202
162 156 84 81
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2041 4749 5191 7872
Size: 2041 2708 442 2681
Fragments arranged by size:
2708 2681 2041 442
TatI w'GTAC_w
Cuts at: 0 211 298 673 2103 4074 4546 6510 7872
Size: 211 87 375 1430 1971 472 1964 1362
Fragments arranged by size:
1971 1964 1430 1362 472 375 211 87
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 6704 6736 7872
Size: 6704 32 1136
Fragments arranged by size:
6704 1136 32
XbaI T'CTAG_A
Cuts at: 0 1267 2664 7345 7872
Size: 1267 1397 4681 527
Fragments arranged by size:
4681 1397 1267 527
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 5589 7872
Size: 5589 2283
XhoI C'TCGA_G
Cuts at: 0 737 7872
Size: 737 7135
XmnI GAAnn'nnTTC
Cuts at: 0 4958 7872
Size: 4958 2914
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AleI AlwNI ApaI
ApaLI ApoI AseI AvaI AvrII BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BfrBI BglII BlpI
Bme1580I BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI
BsrGI BssSI BstBI BstXI BstYI BstZ17I Bsu36I BtgI
BtsI DraI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MmeI MslI MspA1I NarI NcoI NheI NsiI
NspI PasI PciI PfoI PmlI PpuMI PshAI PspOMI
PspXI PsrI PstI SacI SacII SapI ScaI SexAI
SfcI SfoI SmlI SphI SspI StuI StyI TaqII
TatI TstI XbaI XcmI XhoI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AlfI AloI
AscI AsiSI BaeI BamHI BglI BmgBI BsiEI BsiWI
BspEI BsrFI BssHII BstAPI BstEII BtgZI ClaI CspCI
DraIII DrdI EaeI EagI EciI FseI FspI FspAI
MfeI MluI MscI NaeI NdeI NgoMIV NotI NruI
PacI PflMI PmeI PpiI PsiI PvuI PvuII RsrII
SalI SanDI SbfI SfiI SgfI SgrAI SmaI SnaBI
SpeI SrfI SwaI Tth111I XmaI ZraI