Restriction map for
pF1KA1692
(Linear) (Six-base) MAPSORT of: KIAA1692.seq Check: 7190 from: 1 to: 2499
KIAA1692.orf
With 182 enzymes: SgfI *
October 24, 2006 15:09 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1780 2499
Size: 1780 719
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 119 1502 1801 2052 2499
Size: 119 1383 299 251 447
Fragments arranged by size:
1383 447 299 251 119
AflIII A'CryG_T
Cuts at: 0 2057 2499
Size: 2057 442
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2109 2499
Size: 2109 390
AlwNI CAG_nnn'CTG
Cuts at: 0 920 1492 1570 1714 1878 2499
Size: 920 572 78 144 164 621
Fragments arranged by size:
920 621 572 164 144 78
ApaI G_GGCC'C
Cuts at: 0 1030 2499
Size: 1030 1469
ApoI r'AATT_y
Cuts at: 0 403 1150 1557 2499
Size: 403 747 407 942
Fragments arranged by size:
942 747 407 403
AseI AT'TA_AT
Cuts at: 0 587 2499
Size: 587 1912
AvaI C'yCGr_G
Cuts at: 0 691 2354 2499
Size: 691 1663 145
Fragments arranged by size:
1663 691 145
BamHI G'GATC_C
Cuts at: 0 1847 2499
Size: 1847 652
BanII G_rGCy'C
Cuts at: 0 254 1030 1932 2499
Size: 254 776 902 567
Fragments arranged by size:
902 776 567 254
BbsI GAAGACnn'nnnn_
Cuts at: 0 92 308 1205 1244 1508 1739 1948 2499
Size: 92 216 897 39 264 231 209 551
Fragments arranged by size:
897 551 264 231 216 209 92 39
BbvCI CC'TCA_GC
Cuts at: 0 578 1663 1838 2499
Size: 578 1085 175 661
Fragments arranged by size:
1085 661 578 175
BclI T'GATC_A
Cuts at: 0 492 1716 2499
Size: 492 1224 783
Fragments arranged by size:
1224 783 492
BfrBI ATG'CAT
Cuts at: 0 3 2499
Size: 3 2496
BglII A'GATC_T
Cuts at: 0 36 480 2499
Size: 36 444 2019
Fragments arranged by size:
2019 444 36
Bme1580I G_kGCm'C
Cuts at: 0 1030 2499
Size: 1030 1469
BmgBI CAC'GTC
Cuts at: 0 2058 2499
Size: 2058 441
BmrI ACTGGGnnnn_n'
Cuts at: 0 364 2499
Size: 364 2135
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1006 1583 2416 2499
Size: 1006 577 833 83
Fragments arranged by size:
1006 833 577 83
Bpu10I CC'TnA_GC
Cuts at: 0 578 1663 1838 2499
Size: 578 1085 175 661
Fragments arranged by size:
1085 661 578 175
BsaI GGTCTCn'nnnn_
Cuts at: 0 205 276 2499
Size: 205 71 2223
Fragments arranged by size:
2223 205 71
BsaBI GATnn'nnATC
Cuts at: 0 1852 2499
Size: 1852 647
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 46 76 949 979 997 1027 1978 2008
Size: 46 30 873 30 18 30 951 30
Cuts at: 2008 2499
Size: 491
Fragments arranged by size:
951 873 491 46 30 30 30 30
18
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 299 1005 2499
Size: 299 706 1494
Fragments arranged by size:
1494 706 299
BseYI C'CCAG_C
Cuts at: 0 49 1506 2499
Size: 49 1457 993
Fragments arranged by size:
1457 993 49
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 453 2499
Size: 453 2046
BsiHKAI G_wGCw'C
Cuts at: 0 1932 1962 2499
Size: 1932 30 537
Fragments arranged by size:
1932 537 30
BsmI GAATG_Cn'
Cuts at: 0 960 2421 2499
Size: 960 1461 78
Fragments arranged by size:
1461 960 78
Bsp1286I G_dGCh'C
Cuts at: 0 254 1030 1932 1962 2499
Size: 254 776 902 30 537
Fragments arranged by size:
902 776 537 254 30
BspHI T'CATG_A
Cuts at: 0 335 2499
Size: 335 2164
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 564 1780 2499
Size: 564 1216 719
Fragments arranged by size:
1216 719 564
BsrBI CCG'CTC
Cuts at: 0 2345 2499
Size: 2345 154
BsrDI GCAATG_nn'
Cuts at: 0 392 1073 2098 2499
Size: 392 681 1025 401
Fragments arranged by size:
1025 681 401 392
BssSI C'ACGA_G
Cuts at: 0 44 2499
Size: 44 2455
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1957 2499
Size: 1957 542
BstBI TT'CG_AA
Cuts at: 0 2363 2499
Size: 2363 136
BstYI r'GATC_y
Cuts at: 0 36 480 1847 2010 2499
Size: 36 444 1367 163 489
Fragments arranged by size:
1367 489 444 163 36
BtgI C'CryG_G
Cuts at: 0 1685 1843 2499
Size: 1685 158 656
Fragments arranged by size:
1685 656 158
BtsI GCAGTG_nn'
Cuts at: 0 404 2499
Size: 404 2095
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1358 1393 2499
Size: 1358 35 1106
Fragments arranged by size:
1358 1106 35
EaeI y'GGCC_r
Cuts at: 0 947 1455 2142 2324 2499
Size: 947 508 687 182 175
Fragments arranged by size:
947 687 508 182 175
EarI CTCTTCn'nnn_
Cuts at: 0 64 899 2499
Size: 64 835 1600
Fragments arranged by size:
1600 835 64
EcoICRI GAG'CTC
Cuts at: 0 1930 2499
Size: 1930 569
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 119 1006 1502 1583 1801 2052 2416 2499
Size: 119 887 496 81 218 251 364 83
Fragments arranged by size:
887 496 364 251 218 119 83 81
EcoO109I rG'GnC_Cy
Cuts at: 0 54 240 1027 2499
Size: 54 186 787 1472
Fragments arranged by size:
1472 787 186 54
EcoRI G'AATT_C
Cuts at: 0 1150 2499
Size: 1150 1349
EcoRV GAT'ATC
Cuts at: 0 333 2499
Size: 333 2166
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 109 1367 1659 1782 2182 2499
Size: 109 1258 292 123 400 317
Fragments arranged by size:
1258 400 317 292 123 109
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 46 78 202 234 997 1029 1546 1578
Size: 46 32 124 32 763 32 517 32
Cuts at: 1578 2499
Size: 921
Fragments arranged by size:
921 763 517 124 46 32 32 32
32
HindIII A'AGCT_T
Cuts at: 0 101 2499
Size: 101 2398
MscI TGG'CCA
Cuts at: 0 949 1457 2144 2326 2499
Size: 949 508 687 182 173
Fragments arranged by size:
949 687 508 182 173
MslI CAynn'nnrTG
Cuts at: 0 397 899 2091 2443 2499
Size: 397 502 1192 352 56
Fragments arranged by size:
1192 502 397 352 56
MspA1I CmG'CkG
Cuts at: 0 627 1506 2499
Size: 627 879 993
Fragments arranged by size:
993 879 627
NcoI C'CATG_G
Cuts at: 0 1685 1843 2499
Size: 1685 158 656
Fragments arranged by size:
1685 656 158
NdeI CA'TA_TG
Cuts at: 0 1680 2499
Size: 1680 819
NsiI A_TGCA'T
Cuts at: 0 5 2499
Size: 5 2494
NspI r_CATG'y
Cuts at: 0 235 670 898 2499
Size: 235 435 228 1601
Fragments arranged by size:
1601 435 235 228
PpuMI rG'GwC_Cy
Cuts at: 0 54 2499
Size: 54 2445
PspOMI G'GGCC_C
Cuts at: 0 1026 2499
Size: 1026 1473
PspXI vC'TCGA_Gb
Cuts at: 0 691 2499
Size: 691 1808
PvuII CAG'CTG
Cuts at: 0 627 1506 2499
Size: 627 879 993
Fragments arranged by size:
993 879 627
SacI G_AGCT'C
Cuts at: 0 1932 2499
Size: 1932 567
SexAI A'CCwGG_T
Cuts at: 0 932 2018 2499
Size: 932 1086 481
Fragments arranged by size:
1086 932 481
SfcI C'TryA_G
Cuts at: 0 1619 2499
Size: 1619 880
SmaI CCC'GGG
Cuts at: 0 2356 2499
Size: 2356 143
SmlI C'TyrA_G
Cuts at: 0 691 2499
Size: 691 1808
SphI G_CATG'C
Cuts at: 0 235 2499
Size: 235 2264
SspI AAT'ATT
Cuts at: 0 1536 2499
Size: 1536 963
StyI C'CwwG_G
Cuts at: 0 473 525 1109 1458 1685 1843 2402 2499
Size: 473 52 584 349 227 158 559 97
Fragments arranged by size:
584 559 473 349 227 158 97 52
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 229 2499
Size: 229 2270
TatI w'GTAC_w
Cuts at: 0 2304 2499
Size: 2304 195
Tth111I GACn'n_nGTC
Cuts at: 0 216 2499
Size: 216 2283
XhoI C'TCGA_G
Cuts at: 0 691 2499
Size: 691 1808
XmaI C'CCGG_G
Cuts at: 0 2354 2499
Size: 2354 145
Enzymes that do cut:
AarI AcuI AflIII AlfI AlwNI ApaI ApoI AseI
AvaI BamHI BanII BbsI BbvCI BclI BfrBI BglII
Bme1580I BmgBI BmrI BpmI Bpu10I BsaI BsaBI BsaXI
BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspHI BspMI
BsrBI BsrDI BssSI BstAPI BstBI BstYI BtgI BtsI
CspCI EaeI EarI EcoICRI Eco57MI EcoO109I EcoRI EcoRV
FalI Hin4I HindIII MscI MslI MspA1I NcoI NdeI
NsiI NspI PpuMI PspOMI PspXI PvuII SacI SexAI
SfcI SmaI SmlI SphI SspI StyI TaqII TatI
Tth111I XhoI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AleI AloI ApaLI AscI AsiSI AvrII BaeI BanI
BbeI BcgI BciVI BglI BlpI BmtI BplI BpuEI
BsaAI BsaHI BsaWI BsiEI BsiWI BsmBI BspEI BsrFI
BsrGI BssHII BstEII BstXI BstZ17I Bsu36I BtgZI ClaI
DraI DraIII DrdI EagI EciI EcoNI FseI FspI
FspAI HaeII HincII HpaI KasI KpnI MfeI MluI
MmeI NaeI NarI NgoMIV NheI NotI NruI PacI
PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI
PsiI PsrI PstI PvuI RsrII SacII SalI SanDI
SapI SbfI ScaI SfiI SfoI SgfI SgrAI SnaBI
SpeI SrfI StuI SwaI TstI XbaI XcmI XmnI
ZraI