Restriction map for
pF1KSDA1701
(Linear) (Six-base) MAPSORT of: KIAA1701.seq Check: 2840 from: 1 to: 1641
KIAA1701.orf
With 182 enzymes: SgfI *
October 24, 2006 15:17 ..
AatII G_ACGT'C
Cuts at: 0 891 1641
Size: 891 750
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 776 1641
Size: 776 865
AflII C'TTAA_G
Cuts at: 0 994 1641
Size: 994 647
AflIII A'CryG_T
Cuts at: 0 298 1209 1641
Size: 298 911 432
Fragments arranged by size:
911 432 298
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 807 1641
Size: 807 834
AlwNI CAG_nnn'CTG
Cuts at: 0 493 1641
Size: 493 1148
ApoI r'AATT_y
Cuts at: 0 970 1641
Size: 970 671
AseI AT'TA_AT
Cuts at: 0 614 1641
Size: 614 1027
AvrII C'CTAG_G
Cuts at: 0 481 1641
Size: 481 1160
BanI G'GyrC_C
Cuts at: 0 427 708 1641
Size: 427 281 933
Fragments arranged by size:
933 427 281
BanII G_rGCy'C
Cuts at: 0 30 222 1641
Size: 30 192 1419
Fragments arranged by size:
1419 192 30
BbvCI CC'TCA_GC
Cuts at: 0 443 494 596 1641
Size: 443 51 102 1045
Fragments arranged by size:
1045 443 102 51
BclI T'GATC_A
Cuts at: 0 1442 1575 1641
Size: 1442 133 66
Fragments arranged by size:
1442 133 66
Bme1580I G_kGCm'C
Cuts at: 0 432 1641
Size: 432 1209
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 92 665 1641
Size: 92 573 976
Fragments arranged by size:
976 573 92
Bpu10I CC'TnA_GC
Cuts at: 0 443 494 596 1641
Size: 443 51 102 1045
Fragments arranged by size:
1045 443 102 51
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1602 1641
Size: 1602 39
BsaBI GATnn'nnATC
Cuts at: 0 699 1641
Size: 699 942
BsaHI Gr'CG_yC
Cuts at: 0 888 1641
Size: 888 753
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 460 511 514 517 1641
Size: 460 51 3 3 1124
Fragments arranged by size:
1124 460 51 3 3
BseYI C'CCAG_C
Cuts at: 0 4 274 337 463 1641
Size: 4 270 63 126 1178
Fragments arranged by size:
1178 270 126 63 4
BsiHKAI G_wGCw'C
Cuts at: 0 222 1641
Size: 222 1419
BsmI GAATG_Cn'
Cuts at: 0 955 1641
Size: 955 686
BsmBI CGTCTCn'nnnn_
Cuts at: 0 199 1641
Size: 199 1442
Bsp1286I G_dGCh'C
Cuts at: 0 30 222 432 1641
Size: 30 192 210 1209
Fragments arranged by size:
1209 210 192 30
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 465 1641
Size: 465 1176
BsrDI GCAATG_nn'
Cuts at: 0 1046 1641
Size: 1046 595
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1036 1641
Size: 1036 605
BstYI r'GATC_y
Cuts at: 0 700 1641
Size: 700 941
Bsu36I CC'TnA_GG
Cuts at: 0 1362 1641
Size: 1362 279
BtgI C'CryG_G
Cuts at: 0 251 1641
Size: 251 1390
BtsI GCAGTG_nn'
Cuts at: 0 185 1641
Size: 185 1456
DraI TTT'AAA
Cuts at: 0 1406 1641
Size: 1406 235
EaeI y'GGCC_r
Cuts at: 0 1520 1601 1641
Size: 1520 81 40
Fragments arranged by size:
1520 81 40
EarI CTCTTCn'nnn_
Cuts at: 0 338 374 503 1641
Size: 338 36 129 1138
Fragments arranged by size:
1138 338 129 36
EcoICRI GAG'CTC
Cuts at: 0 220 1641
Size: 220 1421
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 92 665 776 1641
Size: 92 573 111 865
Fragments arranged by size:
865 573 111 92
EcoNI CCTnn'n_nnAGG
Cuts at: 0 485 1641
Size: 485 1156
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 746 1522 1641
Size: 746 776 119
Fragments arranged by size:
776 746 119
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 873 878 905 910 1427 1459 1641
Size: 873 5 27 5 517 32 182
Fragments arranged by size:
873 517 182 32 27 5 5
HincII GTy'rAC
Cuts at: 0 1001 1641
Size: 1001 640
HindIII A'AGCT_T
Cuts at: 0 1113 1641
Size: 1113 528
MfeI C'AATT_G
Cuts at: 0 1562 1641
Size: 1562 79
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 431 1447 1641
Size: 431 1016 194
Fragments arranged by size:
1016 431 194
MscI TGG'CCA
Cuts at: 0 1522 1603 1641
Size: 1522 81 38
Fragments arranged by size:
1522 81 38
MspA1I CmG'CkG
Cuts at: 0 685 754 1641
Size: 685 69 887
Fragments arranged by size:
887 685 69
NcoI C'CATG_G
Cuts at: 0 251 1641
Size: 251 1390
NdeI CA'TA_TG
Cuts at: 0 1220 1641
Size: 1220 421
NspI r_CATG'y
Cuts at: 0 302 1213 1641
Size: 302 911 428
Fragments arranged by size:
911 428 302
PciI A'CATG_T
Cuts at: 0 298 1209 1641
Size: 298 911 432
Fragments arranged by size:
911 432 298
PflMI CCAn_nnn'nTGG
Cuts at: 0 638 1641
Size: 638 1003
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 799 831 1641
Size: 799 32 810
Fragments arranged by size:
810 799 32
PsiI TTA'TAA
Cuts at: 0 1589 1641
Size: 1589 52
PstI C_TGCA'G
Cuts at: 0 1431 1641
Size: 1431 210
PvuII CAG'CTG
Cuts at: 0 685 1641
Size: 685 956
SacI G_AGCT'C
Cuts at: 0 222 1641
Size: 222 1419
SfcI C'TryA_G
Cuts at: 0 107 1427 1641
Size: 107 1320 214
Fragments arranged by size:
1320 214 107
SmlI C'TyrA_G
Cuts at: 0 994 1581 1641
Size: 994 587 60
Fragments arranged by size:
994 587 60
SpeI A'CTAG_T
Cuts at: 0 550 1641
Size: 550 1091
StuI AGG'CCT
Cuts at: 0 105 1641
Size: 105 1536
StyI C'CwwG_G
Cuts at: 0 251 269 481 632 1641
Size: 251 18 212 151 1009
Fragments arranged by size:
1009 251 212 151 18
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1033 1076 1641
Size: 1033 43 565
Fragments arranged by size:
1033 565 43
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 815 847 1641
Size: 815 32 794
Fragments arranged by size:
815 794 32
Tth111I GACn'n_nGTC
Cuts at: 0 144 1641
Size: 144 1497
ZraI GAC'GTC
Cuts at: 0 889 1641
Size: 889 752
Enzymes that do cut:
AatII AcuI AflII AflIII AlfI AlwNI ApoI AseI
AvrII BanI BanII BbvCI BclI Bme1580I BpmI Bpu10I
BpuEI BsaBI BsaHI BseRI BseYI BsiHKAI BsmI BsmBI
Bsp1286I BspMI BsrDI BstAPI BstYI Bsu36I BtgI BtsI
DraI EaeI EarI EcoICRI Eco57MI EcoNI FalI Hin4I
HincII HindIII MfeI MmeI MscI MspA1I NcoI NdeI
NspI PciI PflMI PpiI PsiI PstI PvuII SacI
SfcI SmlI SpeI StuI StyI TaqII TstI Tth111I
ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AfeI AgeI AhdI AleI
AloI ApaI ApaLI AscI AsiSI AvaI BaeI BamHI
BbeI BbsI BcgI BciVI BfrBI BglI BglII BlpI
BmgBI BmrI BmtI BplI BsaI BsaAI BsaWI BsaXI
BsgI BsiEI BsiWI BspEI BspHI BsrBI BsrFI BsrGI
BssHII BssSI BstBI BstEII BstXI BstZ17I BtgZI ClaI
CspCI DraIII DrdI EagI EciI EcoO109I EcoRI EcoRV
FseI FspI FspAI HaeII HpaI KasI KpnI MluI
MslI NaeI NarI NgoMIV NheI NotI NruI NsiI
PacI PasI PfoI PmeI PmlI PpuMI PshAI PspOMI
PspXI PsrI PvuI RsrII SacII SalI SanDI SapI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SnaBI SphI SrfI SspI SwaI TatI XbaI XcmI
XhoI XmaI XmnI