Restriction map for
pF1KSDA1704
(Linear) (Six-base) MAPSORT of: KIAA1704.seq Check: 5873 from: 1 to: 1020
KIAA1704.orf
With 182 enzymes: SgfI *
October 24, 2006 15:17 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 212 887 1020
Size: 212 675 133
Fragments arranged by size:
675 212 133
ApaI G_GGCC'C
Cuts at: 0 504 1020
Size: 504 516
AvaI C'yCGr_G
Cuts at: 0 78 1020
Size: 78 942
BanII G_rGCy'C
Cuts at: 0 85 504 1020
Size: 85 419 516
Fragments arranged by size:
516 419 85
BbsI GAAGACnn'nnnn_
Cuts at: 0 158 875 1020
Size: 158 717 145
Fragments arranged by size:
717 158 145
BglII A'GATC_T
Cuts at: 0 915 1020
Size: 915 105
BlpI GC'TnA_GC
Cuts at: 0 782 1020
Size: 782 238
Bme1580I G_kGCm'C
Cuts at: 0 504 1020
Size: 504 516
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 572 662 671 1020
Size: 572 90 9 349
Fragments arranged by size:
572 349 90 9
Bpu10I CC'TnA_GC
Cuts at: 0 701 1020
Size: 701 319
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 904 1020
Size: 904 116
BsaI GGTCTCn'nnnn_
Cuts at: 0 2 1020
Size: 2 1018
BsaBI GATnn'nnATC
Cuts at: 0 819 914 1020
Size: 819 95 106
Fragments arranged by size:
819 106 95
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 660 690 1020
Size: 660 30 330
Fragments arranged by size:
660 330 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 305 1020
Size: 305 715
Bsp1286I G_dGCh'C
Cuts at: 0 85 504 1020
Size: 85 419 516
Fragments arranged by size:
516 419 85
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 777 1020
Size: 777 243
BsrBI CCG'CTC
Cuts at: 0 72 1020
Size: 72 948
BsrDI GCAATG_nn'
Cuts at: 0 340 1020
Size: 340 680
BsrFI r'CCGG_y
Cuts at: 0 21 1020
Size: 21 999
BstYI r'GATC_y
Cuts at: 0 915 1020
Size: 915 105
BtgI C'CryG_G
Cuts at: 0 48 1020
Size: 48 972
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 161 1020
Size: 161 859
BtsI GCAGTG_nn'
Cuts at: 0 426 1020
Size: 426 594
ClaI AT'CG_AT
Cuts at: 0 657 1020
Size: 657 363
DraI TTT'AAA
Cuts at: 0 297 1020
Size: 297 723
EarI CTCTTCn'nnn_
Cuts at: 0 62 627 759 1020
Size: 62 565 132 261
Fragments arranged by size:
565 261 132 62
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 212 572 662 671 887 1020
Size: 212 360 90 9 216 133
Fragments arranged by size:
360 216 212 133 90 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 89 455 1020
Size: 89 366 565
Fragments arranged by size:
565 366 89
EcoO109I rG'GnC_Cy
Cuts at: 0 75 334 500 1020
Size: 75 259 166 520
Fragments arranged by size:
520 259 166 75
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 31 219 251 529 561 631 660 663
Size: 31 188 32 278 32 70 29 3
Cuts at: 663 692 775 807 1020
Size: 29 83 32 213
Fragments arranged by size:
278 213 188 83 70 32 32 32
31 29 29 3
HincII GTy'rAC
Cuts at: 0 468 1020
Size: 468 552
HindIII A'AGCT_T
Cuts at: 0 880 1020
Size: 880 140
HpaI GTT'AAC
Cuts at: 0 468 1020
Size: 468 552
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 235 1020
Size: 235 785
MspA1I CmG'CkG
Cuts at: 0 50 58 682 1020
Size: 50 8 624 338
Fragments arranged by size:
624 338 50 8
PfoI T'CCnGG_A
Cuts at: 0 288 396 1020
Size: 288 108 624
Fragments arranged by size:
624 288 108
PpuMI rG'GwC_Cy
Cuts at: 0 75 334 1020
Size: 75 259 686
Fragments arranged by size:
686 259 75
PshAI GACnn'nnGTC
Cuts at: 0 206 605 1020
Size: 206 399 415
Fragments arranged by size:
415 399 206
PsiI TTA'TAA
Cuts at: 0 116 1020
Size: 116 904
PspOMI G'GGCC_C
Cuts at: 0 500 1020
Size: 500 520
PvuII CAG'CTG
Cuts at: 0 682 1020
Size: 682 338
RsrII CG'GwC_CG
Cuts at: 0 19 1020
Size: 19 1001
SacII CC_GC'GG
Cuts at: 0 51 1020
Size: 51 969
SanDI GG'GwC_CC
Cuts at: 0 75 1020
Size: 75 945
SapI GCTCTTCn'nnn_
Cuts at: 0 62 627 1020
Size: 62 565 393
Fragments arranged by size:
565 393 62
SexAI A'CCwGG_T
Cuts at: 0 348 1020
Size: 348 672
SmlI C'TyrA_G
Cuts at: 0 919 1020
Size: 919 101
StyI C'CwwG_G
Cuts at: 0 319 616 1020
Size: 319 297 404
Fragments arranged by size:
404 319 297
XbaI T'CTAG_A
Cuts at: 0 970 1020
Size: 970 50
Enzymes that do cut:
AcuI ApaI AvaI BanII BbsI BglII BlpI Bme1580I
BpmI Bpu10I BpuEI BsaI BsaBI BsaXI BseRI Bsp1286I
BspMI BsrBI BsrDI BsrFI BstYI BtgI BtgZI BtsI
ClaI DraI EarI Eco57MI EcoNI EcoO109I Hin4I HincII
HindIII HpaI MmeI MspA1I PfoI PpuMI PshAI PsiI
PspOMI PvuII RsrII SacII SanDI SapI SexAI SmlI
StyI XbaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AscI AseI AsiSI AvrII BaeI BamHI BanI BbeI
BbvCI BcgI BciVI BclI BfrBI BglI BmgBI BmrI
BmtI BplI BsaAI BsaHI BsaWI BseYI BsgI BsiEI
BsiHKAI BsiWI BsmI BsmBI BspEI BspHI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I CspCI
DraIII DrdI EaeI EagI EciI EcoICRI EcoRI EcoRV
FalI FseI FspI FspAI HaeII KasI KpnI MfeI
MluI MscI MslI NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI PacI PasI PciI
PflMI PmeI PmlI PpiI PspXI PsrI PstI PvuI
SacI SalI SbfI ScaI SfcI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI
SwaI TaqII TatI TstI Tth111I XcmI XhoI XmaI
XmnI ZraI