Restriction map for
pF1KSDA1710
(Linear) (Six-base) MAPSORT of: KIAA1710.seq Check: 235 from: 1 to: 1410
KIAA1710.orf
With 182 enzymes: SgfI *
February 28, 2007 11:29 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 423 507 759 843 927 1095 1410
Size: 423 84 252 84 84 168 315
Fragments arranged by size:
423 315 252 168 84 84 84
AleI CACnn'nnGTG
Cuts at: 0 734 818 1410
Size: 734 84 592
Fragments arranged by size:
734 592 84
AlwNI CAG_nnn'CTG
Cuts at: 0 367 1410
Size: 367 1043
ApoI r'AATT_y
Cuts at: 0 1023 1145 1410
Size: 1023 122 265
Fragments arranged by size:
1023 265 122
AvaI C'yCGr_G
Cuts at: 0 71 1410
Size: 71 1339
BanI G'GyrC_C
Cuts at: 0 33 1410
Size: 33 1377
BanII G_rGCy'C
Cuts at: 0 787 981 1123 1291 1375 1410
Size: 787 194 142 168 84 35
Fragments arranged by size:
787 194 168 142 84 35
BbsI GAAGACnn'nnnn_
Cuts at: 0 478 1410
Size: 478 932
BbvCI CC'TCA_GC
Cuts at: 0 257 1410
Size: 257 1153
BglII A'GATC_T
Cuts at: 0 446 868 1209 1410
Size: 446 422 341 201
Fragments arranged by size:
446 422 341 201
BlpI GC'TnA_GC
Cuts at: 0 797 1374 1410
Size: 797 577 36
Fragments arranged by size:
797 577 36
BmrI ACTGGGnnnn_n'
Cuts at: 0 575 1410
Size: 575 835
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1394 1410
Size: 1394 16
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 503 671 1091 1175 1410
Size: 503 168 420 84 235
Fragments arranged by size:
503 420 235 168 84
Bpu10I CC'TnA_GC
Cuts at: 0 257 1410
Size: 257 1153
BsaI GGTCTCn'nnnn_
Cuts at: 0 78 1410
Size: 78 1332
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 772 1410
Size: 772 638
BseYI C'CCAG_C
Cuts at: 0 22 1410
Size: 22 1388
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 83 1282 1410
Size: 83 1199 128
Fragments arranged by size:
1199 128 83
BsiHKAI G_wGCw'C
Cuts at: 0 787 1123 1291 1375 1410
Size: 787 336 168 84 35
Fragments arranged by size:
787 336 168 84 35
BsmI GAATG_Cn'
Cuts at: 0 258 1093 1410
Size: 258 835 317
Fragments arranged by size:
835 317 258
BsmBI CGTCTCn'nnnn_
Cuts at: 0 703 1410
Size: 703 707
Bsp1286I G_dGCh'C
Cuts at: 0 787 981 1123 1291 1375 1410
Size: 787 194 142 168 84 35
Fragments arranged by size:
787 194 168 142 84 35
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 252 1410
Size: 252 1158
BsrDI GCAATG_nn'
Cuts at: 0 1098 1410
Size: 1098 312
BsrGI T'GTAC_A
Cuts at: 0 216 1410
Size: 216 1194
BstYI r'GATC_y
Cuts at: 0 446 868 1209 1410
Size: 446 422 341 201
Fragments arranged by size:
446 422 341 201
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 955 1410
Size: 955 455
BtsI GCAGTG_nn'
Cuts at: 0 1270 1410
Size: 1270 140
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1080 1115 1410
Size: 1080 35 295
Fragments arranged by size:
1080 295 35
EaeI y'GGCC_r
Cuts at: 0 56 849 1410
Size: 56 793 561
Fragments arranged by size:
793 561 56
EarI CTCTTCn'nnn_
Cuts at: 0 272 329 1351 1410
Size: 272 57 1022 59
Fragments arranged by size:
1022 272 59 57
EcoICRI GAG'CTC
Cuts at: 0 785 1121 1289 1373 1410
Size: 785 336 168 84 37
Fragments arranged by size:
785 336 168 84 37
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 423 503 507 671 759 843 927 1091
Size: 423 80 4 164 88 84 84 164
Cuts at: 1091 1095 1175 1410
Size: 4 80 235
Fragments arranged by size:
423 235 164 164 88 84 84 80
80 4 4
EcoO109I rG'GnC_Cy
Cuts at: 0 26 108 725 809 911 1410
Size: 26 82 617 84 102 499
Fragments arranged by size:
617 499 102 84 82 26
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 618 650 858 860 890 892 1110 1142
Size: 618 32 208 2 30 2 218 32
Cuts at: 1142 1410
Size: 268
Fragments arranged by size:
618 268 218 208 32 32 30 2
2
HindIII A'AGCT_T
Cuts at: 0 771 1107 1191 1410
Size: 771 336 84 219
Fragments arranged by size:
771 336 219 84
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 381 738 980 1316 1410
Size: 381 357 242 336 94
Fragments arranged by size:
381 357 336 242 94
MscI TGG'CCA
Cuts at: 0 58 1410
Size: 58 1352
MslI CAynn'nnrTG
Cuts at: 0 734 818 1086 1410
Size: 734 84 268 324
Fragments arranged by size:
734 324 268 84
NdeI CA'TA_TG
Cuts at: 0 1083 1410
Size: 1083 327
PpuMI rG'GwC_Cy
Cuts at: 0 26 108 725 809 1410
Size: 26 82 617 84 601
Fragments arranged by size:
617 601 84 82 26
SacI G_AGCT'C
Cuts at: 0 787 1123 1291 1375 1410
Size: 787 336 168 84 35
Fragments arranged by size:
787 336 168 84 35
SanDI GG'GwC_CC
Cuts at: 0 26 1410
Size: 26 1384
SmaI CCC'GGG
Cuts at: 0 73 1410
Size: 73 1337
StuI AGG'CCT
Cuts at: 0 436 576 1410
Size: 436 140 834
Fragments arranged by size:
834 436 140
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1297 1410
Size: 1297 113
TatI w'GTAC_w
Cuts at: 0 216 1410
Size: 216 1194
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 717 749 1410
Size: 717 32 661
Fragments arranged by size:
717 661 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 15 1410
Size: 15 1395
XmaI C'CCGG_G
Cuts at: 0 71 1410
Size: 71 1339
XmnI GAAnn'nnTTC
Cuts at: 0 615 772 940 1108 1276 1360 1410
Size: 615 157 168 168 168 84 50
Fragments arranged by size:
615 168 168 168 157 84 50
Enzymes that do cut:
AcuI AleI AlwNI ApoI AvaI BanI BanII BbsI
BbvCI BglII BlpI BmrI BplI BpmI Bpu10I BsaI
BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspMI
BsrDI BsrGI BstYI BtgZI BtsI CspCI EaeI EarI
EcoICRI Eco57MI EcoO109I Hin4I HindIII MmeI MscI MslI
NdeI PpuMI SacI SanDI SmaI StuI TaqII TatI
TstI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AlfI AloI ApaI ApaLI AscI AseI
AsiSI AvrII BaeI BamHI BbeI BcgI BciVI BclI
BfrBI BglI Bme1580I BmgBI BmtI BpuEI BsaAI BsaBI
BsaHI BsaWI BsaXI BsiEI BsiWI BspEI BspHI BsrBI
BsrFI BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I
Bsu36I BtgI ClaI DraI DraIII DrdI EagI EciI
EcoNI EcoRI EcoRV FalI FseI FspI FspAI HaeII
HincII HpaI KasI KpnI MfeI MluI MspA1I NaeI
NarI NcoI NgoMIV NheI NotI NruI NsiI NspI
PacI PasI PciI PflMI PfoI PmeI PmlI PpiI
PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII
RsrII SacII SalI SapI SbfI ScaI SexAI SfcI
SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI SphI
SrfI SspI StyI SwaI Tth111I XbaI XhoI ZraI