Restriction map for
pF1KSDA1720
(Linear) (Six-base) MAPSORT of: KIAA1720.seq Check: 5334 from: 1 to: 1179
KIAA1720.orf
With 182 enzymes: SgfI *
October 24, 2006 15:17 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 80 1010 1179
Size: 80 930 169
Fragments arranged by size:
930 169 80
AfeI AGC'GCT
Cuts at: 0 401 1179
Size: 401 778
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 452 1179
Size: 452 727
AlwNI CAG_nnn'CTG
Cuts at: 0 705 1179
Size: 705 474
ApaI G_GGCC'C
Cuts at: 0 856 971 1179
Size: 856 115 208
Fragments arranged by size:
856 208 115
AvaI C'yCGr_G
Cuts at: 0 440 617 892 1179
Size: 440 177 275 287
Fragments arranged by size:
440 287 275 177
AvrII C'CTAG_G
Cuts at: 0 82 1179
Size: 82 1097
BamHI G'GATC_C
Cuts at: 0 168 269 1179
Size: 168 101 910
Fragments arranged by size:
910 168 101
BanI G'GyrC_C
Cuts at: 0 821 1121 1157 1179
Size: 821 300 36 22
Fragments arranged by size:
821 300 36 22
BanII G_rGCy'C
Cuts at: 0 11 91 683 856 971 1146 1179
Size: 11 80 592 173 115 175 33
Fragments arranged by size:
592 175 173 115 80 33 11
BbeI G_GCGC'C
Cuts at: 0 825 1125 1179
Size: 825 300 54
Fragments arranged by size:
825 300 54
BbsI GAAGACnn'nnnn_
Cuts at: 0 643 1179
Size: 643 536
BbvCI CC'TCA_GC
Cuts at: 0 611 1179
Size: 611 568
BlpI GC'TnA_GC
Cuts at: 0 5 377 461 1006 1179
Size: 5 372 84 545 173
Fragments arranged by size:
545 372 173 84 5
Bme1580I G_kGCm'C
Cuts at: 0 856 971 1179
Size: 856 115 208
Fragments arranged by size:
856 208 115
BmgBI CAC'GTC
Cuts at: 0 1080 1179
Size: 1080 99
BmrI ACTGGGnnnn_n'
Cuts at: 0 85 684 1179
Size: 85 599 495
Fragments arranged by size:
599 495 85
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 128 727 757 811 967 1179
Size: 128 599 30 54 156 212
Fragments arranged by size:
599 212 156 128 54 30
Bpu10I CC'TnA_GC
Cuts at: 0 368 611 752 1179
Size: 368 243 141 427
Fragments arranged by size:
427 368 243 141
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 667 1179
Size: 667 512
BsaI GGTCTCn'nnnn_
Cuts at: 0 28 1179
Size: 28 1151
BsaAI yAC'GTr
Cuts at: 0 503 1179
Size: 503 676
BsaHI Gr'CG_yC
Cuts at: 0 470 822 1122 1179
Size: 470 352 300 57
Fragments arranged by size:
470 352 300 57
BsaWI w'CCGG_w
Cuts at: 0 644 1179
Size: 644 535
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 125 169 199 229 724 855 964 1179
Size: 125 44 30 30 495 131 109 215
Fragments arranged by size:
495 215 131 125 109 44 30 30
BseYI C'CCAG_C
Cuts at: 0 1101 1179
Size: 1101 78
BsiHKAI G_wGCw'C
Cuts at: 0 11 197 581 683 716 1058 1179
Size: 11 186 384 102 33 342 121
Fragments arranged by size:
384 342 186 121 102 33 11
Bsp1286I G_dGCh'C
Cuts at: 0 11 91 197 581 683 716 856 971
Size: 11 80 106 384 102 33 140 115
Cuts at: 971 1058 1146 1179
Size: 87 88 33
Fragments arranged by size:
384 140 115 106 102 88 87 80
33 33 11
BspEI T'CCGG_A
Cuts at: 0 644 1179
Size: 644 535
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 538 735 1040 1179
Size: 538 197 305 139
Fragments arranged by size:
538 305 197 139
BsrBI CCG'CTC
Cuts at: 0 414 558 573 1179
Size: 414 144 15 606
Fragments arranged by size:
606 414 144 15
BstAPI GCAn_nnn'nTGC
Cuts at: 0 513 1179
Size: 513 666
BstYI r'GATC_y
Cuts at: 0 168 269 701 1179
Size: 168 101 432 478
Fragments arranged by size:
478 432 168 101
BtgI C'CryG_G
Cuts at: 0 773 825 872 1049 1146 1179
Size: 773 52 47 177 97 33
Fragments arranged by size:
773 177 97 52 47 33
BtsI GCAGTG_nn'
Cuts at: 0 135 1179
Size: 135 1044
EaeI y'GGCC_r
Cuts at: 0 1092 1179
Size: 1092 87
EarI CTCTTCn'nnn_
Cuts at: 0 95 548 1179
Size: 95 453 631
Fragments arranged by size:
631 453 95
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 562 1154 1179
Size: 562 592 25
Fragments arranged by size:
592 562 25
EcoICRI GAG'CTC
Cuts at: 0 9 681 1179
Size: 9 672 498
Fragments arranged by size:
672 498 9
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 80 128 727 757 811 967 1010 1179
Size: 80 48 599 30 54 156 43 169
Fragments arranged by size:
599 169 156 80 54 48 43 30
EcoNI CCTnn'n_nnAGG
Cuts at: 0 110 202 264 1179
Size: 110 92 62 915
Fragments arranged by size:
915 110 92 62
EcoO109I rG'GnC_Cy
Cuts at: 0 853 889 967 1179
Size: 853 36 78 212
Fragments arranged by size:
853 212 78 36
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 577 1179
Size: 577 602
FspI TGC'GCA
Cuts at: 0 1015 1179
Size: 1015 164
HaeII r_GCGC'y
Cuts at: 0 403 825 864 1125 1179
Size: 403 422 39 261 54
Fragments arranged by size:
422 403 261 54 39
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 246 278 1179
Size: 246 32 901
Fragments arranged by size:
901 246 32
KasI G'GCGC_C
Cuts at: 0 821 1121 1179
Size: 821 300 58
Fragments arranged by size:
821 300 58
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 170 1179
Size: 170 1009
MspA1I CmG'CkG
Cuts at: 0 240 827 917 1179
Size: 240 587 90 262
Fragments arranged by size:
587 262 240 90
NarI GG'CG_CC
Cuts at: 0 822 1122 1179
Size: 822 300 57
Fragments arranged by size:
822 300 57
NspI r_CATG'y
Cuts at: 0 429 1179
Size: 429 750
PasI CC'CwG_GG
Cuts at: 0 849 1179
Size: 849 330
PflMI CCAn_nnn'nTGG
Cuts at: 0 705 849 1101 1179
Size: 705 144 252 78
Fragments arranged by size:
705 252 144 78
PfoI T'CCnGG_A
Cuts at: 0 21 704 1179
Size: 21 683 475
Fragments arranged by size:
683 475 21
PmlI CAC'GTG
Cuts at: 0 503 1179
Size: 503 676
PpuMI rG'GwC_Cy
Cuts at: 0 889 1179
Size: 889 290
PspOMI G'GGCC_C
Cuts at: 0 852 967 1179
Size: 852 115 212
Fragments arranged by size:
852 212 115
PstI C_TGCA'G
Cuts at: 0 635 1037 1179
Size: 635 402 142
Fragments arranged by size:
635 402 142
PvuII CAG'CTG
Cuts at: 0 240 1179
Size: 240 939
SacI G_AGCT'C
Cuts at: 0 11 683 1179
Size: 11 672 496
Fragments arranged by size:
672 496 11
SacII CC_GC'GG
Cuts at: 0 828 1179
Size: 828 351
SapI GCTCTTCn'nnn_
Cuts at: 0 95 548 1179
Size: 95 453 631
Fragments arranged by size:
631 453 95
SexAI A'CCwGG_T
Cuts at: 0 224 1179
Size: 224 955
SfcI C'TryA_G
Cuts at: 0 235 274 631 1033 1179
Size: 235 39 357 402 146
Fragments arranged by size:
402 357 235 146 39
SfoI GGC'GCC
Cuts at: 0 823 1123 1179
Size: 823 300 56
Fragments arranged by size:
823 300 56
SmlI C'TyrA_G
Cuts at: 0 440 682 1179
Size: 440 242 497
Fragments arranged by size:
497 440 242
SphI G_CATG'C
Cuts at: 0 429 1179
Size: 429 750
StuI AGG'CCT
Cuts at: 0 1067 1179
Size: 1067 112
StyI C'CwwG_G
Cuts at: 0 82 722 1179
Size: 82 640 457
Fragments arranged by size:
640 457 82
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 487 519 1179
Size: 487 32 660
Fragments arranged by size:
660 487 32
Tth111I GACn'n_nGTC
Cuts at: 0 1078 1179
Size: 1078 101
XhoI C'TCGA_G
Cuts at: 0 440 1179
Size: 440 739
Enzymes that do cut:
AcuI AfeI AlfI AlwNI ApaI AvaI AvrII BamHI
BanI BanII BbeI BbsI BbvCI BlpI Bme1580I BmgBI
BmrI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI
BseRI BseYI BsiHKAI Bsp1286I BspEI BspMI BsrBI BstAPI
BstYI BtgI BtsI EaeI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I FalI FspI HaeII Hin4I KasI MmeI
MspA1I NarI NspI PasI PflMI PfoI PmlI PpuMI
PspOMI PstI PvuII SacI SacII SapI SexAI SfcI
SfoI SmlI SphI StuI StyI TstI Tth111I XhoI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AflIII AgeI
AhdI AleI AloI ApaLI ApoI AscI AseI AsiSI
BaeI BcgI BciVI BclI BfrBI BglI BglII BmtI
BplI BsaBI BsaXI BsgI BsiEI BsiWI BsmI BsmBI
BspHI BsrDI BsrFI BsrGI BssHII BssSI BstBI BstEII
BstXI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII
DrdI EagI EcoRI EcoRV FseI FspAI HincII HindIII
HpaI KpnI MfeI MluI MscI MslI NaeI NcoI
NdeI NgoMIV NheI NotI NruI NsiI PacI PciI
PmeI PpiI PshAI PsiI PspXI PsrI PvuI RsrII
SalI SanDI SbfI ScaI SfiI SgfI SgrAI SmaI
SnaBI SpeI SrfI SspI SwaI TaqII TatI XbaI
XcmI XmaI XmnI ZraI