Restriction map for
pF1KSDA1722
(Linear) (Six-base) MAPSORT of: KIAA1722.seq Check: 9442 from: 1 to: 4497
KIAA1722.orf
With 182 enzymes: SgfI *
October 24, 2006 15:17 ..
AccI GT'mk_AC
Cuts at: 0 926 1415 4497
Size: 926 489 3082
Fragments arranged by size:
3082 926 489
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2167 2395 3507 4497
Size: 2167 228 1112 990
Fragments arranged by size:
2167 1112 990 228
AflII C'TTAA_G
Cuts at: 0 651 2697 4069 4497
Size: 651 2046 1372 428
Fragments arranged by size:
2046 1372 651 428
AleI CACnn'nnGTG
Cuts at: 0 727 4497
Size: 727 3770
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 352 745 3481 4497
Size: 352 393 2736 1016
Fragments arranged by size:
2736 1016 393 352
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 4268 4300 4497
Size: 4268 32 197
Fragments arranged by size:
4268 197 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1401 4461 4497
Size: 1401 3060 36
Fragments arranged by size:
3060 1401 36
ApaI G_GGCC'C
Cuts at: 0 3554 4497
Size: 3554 943
ApoI r'AATT_y
Cuts at: 0 29 292 1123 1471 1761 4087 4497
Size: 29 263 831 348 290 2326 410
Fragments arranged by size:
2326 831 410 348 290 263 29
AseI AT'TA_AT
Cuts at: 0 2098 2237 4497
Size: 2098 139 2260
Fragments arranged by size:
2260 2098 139
AvaI C'yCGr_G
Cuts at: 0 132 1826 2912 4497
Size: 132 1694 1086 1585
Fragments arranged by size:
1694 1585 1086 132
BanI G'GyrC_C
Cuts at: 0 3744 3955 4336 4497
Size: 3744 211 381 161
Fragments arranged by size:
3744 381 211 161
BanII G_rGCy'C
Cuts at: 0 1854 3554 4331 4497
Size: 1854 1700 777 166
Fragments arranged by size:
1854 1700 777 166
BbeI G_GCGC'C
Cuts at: 0 4340 4497
Size: 4340 157
BbsI GAAGACnn'nnnn_
Cuts at: 0 2999 3206 3638 4104 4497
Size: 2999 207 432 466 393
Fragments arranged by size:
2999 466 432 393 207
BbvCI CC'TCA_GC
Cuts at: 0 172 684 1267 4497
Size: 172 512 583 3230
Fragments arranged by size:
3230 583 512 172
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 105 139 2851 2885 4497
Size: 105 34 2712 34 1612
Fragments arranged by size:
2712 1612 105 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1215 4497
Size: 1215 3282
BclI T'GATC_A
Cuts at: 0 303 3897 4497
Size: 303 3594 600
Fragments arranged by size:
3594 600 303
BglI GCCn_nnn'nGGC
Cuts at: 0 2599 4497
Size: 2599 1898
BglII A'GATC_T
Cuts at: 0 2795 4497
Size: 2795 1702
BlpI GC'TnA_GC
Cuts at: 0 1529 4497
Size: 1529 2968
Bme1580I G_kGCm'C
Cuts at: 0 3323 3554 3749 4297 4497
Size: 3323 231 195 548 200
Fragments arranged by size:
3323 548 231 200 195
BmrI ACTGGGnnnn_n'
Cuts at: 0 234 3369 4437 4497
Size: 234 3135 1068 60
Fragments arranged by size:
3135 1068 234 60
BmtI G_CTAG'C
Cuts at: 0 2177 2318 4497
Size: 2177 141 2179
Fragments arranged by size:
2179 2177 141
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 4203 4497
Size: 4203 294
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 268 442 652 890 1339 1423 1537 3067
Size: 268 174 210 238 449 84 114 1530
Cuts at: 3067 3752 4497
Size: 685 745
Fragments arranged by size:
1530 745 685 449 268 238 210 174
114 84
Bpu10I CC'TnA_GC
Cuts at: 0 172 684 1081 1267 4497
Size: 172 512 397 186 3230
Fragments arranged by size:
3230 512 397 186 172
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 517 772 967 2720 2812 4497
Size: 517 255 195 1753 92 1685
Fragments arranged by size:
1753 1685 517 255 195 92
BsaI GGTCTCn'nnnn_
Cuts at: 0 673 1147 4209 4497
Size: 673 474 3062 288
Fragments arranged by size:
3062 673 474 288
BsaAI yAC'GTr
Cuts at: 0 2611 4497
Size: 2611 1886
BsaBI GATnn'nnATC
Cuts at: 0 2339 4497
Size: 2339 2158
BsaHI Gr'CG_yC
Cuts at: 0 1957 4238 4337 4497
Size: 1957 2281 99 160
Fragments arranged by size:
2281 1957 160 99
BsaWI w'CCGG_w
Cuts at: 0 1748 3512 3557 4497
Size: 1748 1764 45 940
Fragments arranged by size:
1764 1748 940 45
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 148 178 182 212 1589 1619 2509 2539
Size: 148 30 4 30 1377 30 890 30
Cuts at: 2539 3298 3328 4042 4072 4497
Size: 759 30 714 30 425
Fragments arranged by size:
1377 890 759 714 425 148 30 30
30 30 30 30 4
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 2132 2740 3583 3657 4497
Size: 2132 608 843 74 840
Fragments arranged by size:
2132 843 840 608 74
BseYI C'CCAG_C
Cuts at: 0 414 1534 1678 1686 2055 2780 3492 3546
Size: 414 1120 144 8 369 725 712 54
Cuts at: 3546 4345 4472 4497
Size: 799 127 25
Fragments arranged by size:
1120 799 725 712 414 369 144 127
54 25 8
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2405 2467 2650 4497
Size: 2405 62 183 1847
Fragments arranged by size:
2405 1847 183 62
BsiEI CG_ry'CG
Cuts at: 0 1741 4047 4497
Size: 1741 2306 450
Fragments arranged by size:
2306 1741 450
BsiHKAI G_wGCw'C
Cuts at: 0 1715 1854 3835 4497
Size: 1715 139 1981 662
Fragments arranged by size:
1981 1715 662 139
Bsp1286I G_dGCh'C
Cuts at: 0 1715 1854 3323 3554 3749 3835 4297 4331
Size: 1715 139 1469 231 195 86 462 34
Cuts at: 4331 4497
Size: 166
Fragments arranged by size:
1715 1469 462 231 195 166 139 86
34
BspEI T'CCGG_A
Cuts at: 0 3557 4497
Size: 3557 940
BspHI T'CATG_A
Cuts at: 0 4169 4497
Size: 4169 328
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2951 4497
Size: 2951 1546
BsrBI CCG'CTC
Cuts at: 0 242 621 2928 4497
Size: 242 379 2307 1569
Fragments arranged by size:
2307 1569 379 242
BsrFI r'CCGG_y
Cuts at: 0 2060 2546 4497
Size: 2060 486 1951
Fragments arranged by size:
2060 1951 486
BsrGI T'GTAC_A
Cuts at: 0 1858 3470 4497
Size: 1858 1612 1027
Fragments arranged by size:
1858 1612 1027
BstAPI GCAn_nnn'nTGC
Cuts at: 0 4059 4461 4497
Size: 4059 402 36
Fragments arranged by size:
4059 402 36
BstXI CCAn_nnnn'nTGG
Cuts at: 0 588 3193 4497
Size: 588 2605 1304
Fragments arranged by size:
2605 1304 588
BstYI r'GATC_y
Cuts at: 0 691 2373 2795 3162 4497
Size: 691 1682 422 367 1335
Fragments arranged by size:
1682 1335 691 422 367
BstZ17I GTA'TAC
Cuts at: 0 1416 4497
Size: 1416 3081
Bsu36I CC'TnA_GG
Cuts at: 0 1909 3233 3664 4497
Size: 1909 1324 431 833
Fragments arranged by size:
1909 1324 833 431
BtgI C'CryG_G
Cuts at: 0 1159 1974 3269 3637 3947 4361 4497
Size: 1159 815 1295 368 310 414 136
Fragments arranged by size:
1295 1159 815 414 368 310 136
BtsI GCAGTG_nn'
Cuts at: 0 3001 4297 4459 4497
Size: 3001 1296 162 38
Fragments arranged by size:
3001 1296 162 38
DraI TTT'AAA
Cuts at: 0 1308 1590 2814 4497
Size: 1308 282 1224 1683
Fragments arranged by size:
1683 1308 1224 282
DraIII CAC_nnn'GTG
Cuts at: 0 279 4497
Size: 279 4218
DrdI GACnn_nn'nnGTC
Cuts at: 0 3607 3994 4497
Size: 3607 387 503
Fragments arranged by size:
3607 503 387
EaeI y'GGCC_r
Cuts at: 0 1738 1835 3700 4497
Size: 1738 97 1865 797
Fragments arranged by size:
1865 1738 797 97
EagI C'GGCC_G
Cuts at: 0 1738 4497
Size: 1738 2759
EarI CTCTTCn'nnn_
Cuts at: 0 1145 1352 2032 2888 3958 4497
Size: 1145 207 680 856 1070 539
Fragments arranged by size:
1145 1070 856 680 539 207
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 3104 4497
Size: 3104 1393
EcoICRI GAG'CTC
Cuts at: 0 1852 4497
Size: 1852 2645
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 268 442 652 890 1339 1423 1537 2167
Size: 268 174 210 238 449 84 114 630
Cuts at: 2167 2395 3067 3507 3752 4497
Size: 228 672 440 245 745
Fragments arranged by size:
745 672 630 449 440 268 245 238
228 210 174 114 84
EcoNI CCTnn'n_nnAGG
Cuts at: 0 4072 4138 4497
Size: 4072 66 359
Fragments arranged by size:
4072 359 66
EcoO109I rG'GnC_Cy
Cuts at: 0 3550 4497
Size: 3550 947
EcoRI G'AATT_C
Cuts at: 0 29 1123 4497
Size: 29 1094 3374
Fragments arranged by size:
3374 1094 29
EcoRV GAT'ATC
Cuts at: 0 2964 4497
Size: 2964 1533
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1837 2307 4497
Size: 1837 470 2190
Fragments arranged by size:
2190 1837 470
HaeII r_GCGC'y
Cuts at: 0 2675 4340 4497
Size: 2675 1665 157
Fragments arranged by size:
2675 1665 157
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 148 180 1710 1742 1836 1868 2912 2944
Size: 148 32 1530 32 94 32 1044 32
Cuts at: 2944 3049 3081 3406 3438 3565 3597 3864 3896
Size: 105 32 325 32 127 32 267 32
Cuts at: 3896 4497
Size: 601
Fragments arranged by size:
1530 1044 601 325 267 148 127 105
94 32 32 32 32 32 32 32
32
HincII GTy'rAC
Cuts at: 0 2109 2164 4497
Size: 2109 55 2333
Fragments arranged by size:
2333 2109 55
HindIII A'AGCT_T
Cuts at: 0 370 4497
Size: 370 4127
HpaI GTT'AAC
Cuts at: 0 2109 4497
Size: 2109 2388
KasI G'GCGC_C
Cuts at: 0 4336 4497
Size: 4336 161
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 3404 4497
Size: 3404 1093
MscI TGG'CCA
Cuts at: 0 1837 4497
Size: 1837 2660
MslI CAynn'nnrTG
Cuts at: 0 49 586 727 3191 4210 4497
Size: 49 537 141 2464 1019 287
Fragments arranged by size:
2464 1019 537 287 141 49
MspA1I CmG'CkG
Cuts at: 0 382 414 1682 3389 3639 3857 4497
Size: 382 32 1268 1707 250 218 640
Fragments arranged by size:
1707 1268 640 382 250 218 32
NaeI GCC'GGC
Cuts at: 0 2062 4497
Size: 2062 2435
NarI GG'CG_CC
Cuts at: 0 4337 4497
Size: 4337 160
NcoI C'CATG_G
Cuts at: 0 1159 3269 4497
Size: 1159 2110 1228
Fragments arranged by size:
2110 1228 1159
NdeI CA'TA_TG
Cuts at: 0 2855 4497
Size: 2855 1642
NgoMIV G'CCGG_C
Cuts at: 0 2060 4497
Size: 2060 2437
NheI G'CTAG_C
Cuts at: 0 2173 2314 4497
Size: 2173 141 2183
Fragments arranged by size:
2183 2173 141
NspI r_CATG'y
Cuts at: 0 1676 4011 4066 4497
Size: 1676 2335 55 431
Fragments arranged by size:
2335 1676 431 55
PasI CC'CwG_GG
Cuts at: 0 3585 4497
Size: 3585 912
PflMI CCAn_nnn'nTGG
Cuts at: 0 675 1401 3912 4497
Size: 675 726 2511 585
Fragments arranged by size:
2511 726 675 585
PfoI T'CCnGG_A
Cuts at: 0 1341 2273 2684 4497
Size: 1341 932 411 1813
Fragments arranged by size:
1813 1341 932 411
PsiI TTA'TAA
Cuts at: 0 3593 4497
Size: 3593 904
PspOMI G'GGCC_C
Cuts at: 0 3550 4497
Size: 3550 947
PstI C_TGCA'G
Cuts at: 0 341 1236 2948 3887 4497
Size: 341 895 1712 939 610
Fragments arranged by size:
1712 939 895 610 341
PvuII CAG'CTG
Cuts at: 0 382 414 1682 4497
Size: 382 32 1268 2815
Fragments arranged by size:
2815 1268 382 32
SacI G_AGCT'C
Cuts at: 0 1854 4497
Size: 1854 2643
SacII CC_GC'GG
Cuts at: 0 3640 4497
Size: 3640 857
SapI GCTCTTCn'nnn_
Cuts at: 0 3958 4497
Size: 3958 539
SbfI CC_TGCA'GG
Cuts at: 0 2948 4497
Size: 2948 1549
SexAI A'CCwGG_T
Cuts at: 0 2297 4497
Size: 2297 2200
SfcI C'TryA_G
Cuts at: 0 337 812 964 1232 2675 2769 2880 2944
Size: 337 475 152 268 1443 94 111 64
Cuts at: 2944 3883 4497
Size: 939 614
Fragments arranged by size:
1443 939 614 475 337 268 152 111
94 64
SfoI GGC'GCC
Cuts at: 0 4338 4497
Size: 4338 159
SmaI CCC'GGG
Cuts at: 0 2914 4497
Size: 2914 1583
SmlI C'TyrA_G
Cuts at: 0 532 651 787 982 2697 2735 2791 4069
Size: 532 119 136 195 1715 38 56 1278
Cuts at: 4069 4497
Size: 428
Fragments arranged by size:
1715 1278 532 428 195 136 119 56
38
SphI G_CATG'C
Cuts at: 0 4066 4497
Size: 4066 431
SspI AAT'ATT
Cuts at: 0 1495 3651 4497
Size: 1495 2156 846
Fragments arranged by size:
2156 1495 846
StuI AGG'CCT
Cuts at: 0 901 2593 2878 4497
Size: 901 1692 285 1619
Fragments arranged by size:
1692 1619 901 285
StyI C'CwwG_G
Cuts at: 0 1159 1351 3180 3269 3289 3336 4497
Size: 1159 192 1829 89 20 47 1161
Fragments arranged by size:
1829 1161 1159 192 89 47 20
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 85 1540 1673 2819 3349 4449 4497
Size: 85 1455 133 1146 530 1100 48
Fragments arranged by size:
1455 1146 1100 530 133 85 48
TatI w'GTAC_w
Cuts at: 0 395 1858 3470 3809 4497
Size: 395 1463 1612 339 688
Fragments arranged by size:
1612 1463 688 395 339
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 182 214 4497
Size: 182 32 4283
Fragments arranged by size:
4283 182 32
Tth111I GACn'n_nGTC
Cuts at: 0 922 4497
Size: 922 3575
XbaI T'CTAG_A
Cuts at: 0 1062 3426 4497
Size: 1062 2364 1071
Fragments arranged by size:
2364 1071 1062
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 94 702 3276 3525 4497
Size: 94 608 2574 249 972
Fragments arranged by size:
2574 972 608 249 94
XmaI C'CCGG_G
Cuts at: 0 2912 4497
Size: 2912 1585
XmnI GAAnn'nnTTC
Cuts at: 0 1202 1472 1948 4497
Size: 1202 270 476 2549
Fragments arranged by size:
2549 1202 476 270
Enzymes that do cut:
AccI AcuI AflII AleI AlfI AloI AlwNI ApaI
ApoI AseI AvaI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrFI BsrGI BstAPI
BstXI BstYI BstZ17I Bsu36I BtgI BtsI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI HaeII Hin4I HincII HindIII
HpaI KasI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NspI PasI PflMI PfoI
PsiI PspOMI PstI PvuII SacI SacII SapI SbfI
SexAI SfcI SfoI SmaI SmlI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflIII AgeI AhdI
ApaLI AscI AsiSI AvrII BaeI BamHI BfrBI BmgBI
BsiWI BsmI BsmBI BsrDI BssHII BssSI BstBI BstEII
BtgZI ClaI CspCI FseI FspI FspAI KpnI MfeI
MluI NotI NruI NsiI PacI PciI PmeI PmlI
PpiI PpuMI PshAI PspXI PsrI PvuI RsrII SalI
SanDI ScaI SfiI SgfI SgrAI SnaBI SpeI SrfI
SwaI XhoI ZraI