Restriction map for
pF1KSDA1723
(Linear) (Six-base) MAPSORT of: KIAA1723.seq Check: 1254 from: 1 to: 4584
KIAA1723.orf
With 182 enzymes: SgfI *
October 24, 2006 15:17 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 754 4584
Size: 754 3830
AccI GT'mk_AC
Cuts at: 0 4181 4584
Size: 4181 403
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1929 1935 2041 2143 2450 2608 3321 3469
Size: 1929 6 106 102 307 158 713 148
Cuts at: 3469 3736 3925 4527 4538 4584
Size: 267 189 602 11 46
Fragments arranged by size:
1929 713 602 307 267 189 158 148
106 102 46 11 6
AflII C'TTAA_G
Cuts at: 0 594 4461 4584
Size: 594 3867 123
Fragments arranged by size:
3867 594 123
AflIII A'CryG_T
Cuts at: 0 36 4445 4584
Size: 36 4409 139
Fragments arranged by size:
4409 139 36
AhdI GACnn_n'nnGTC
Cuts at: 0 1251 1994 4584
Size: 1251 743 2590
Fragments arranged by size:
2590 1251 743
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2401 2433 4584
Size: 2401 32 2151
Fragments arranged by size:
2401 2151 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1189 3372 3546 3609 4584
Size: 1189 2183 174 63 975
Fragments arranged by size:
2183 1189 975 174 63
ApaI G_GGCC'C
Cuts at: 0 2090 4584
Size: 2090 2494
ApoI r'AATT_y
Cuts at: 0 1233 3726 4208 4229 4584
Size: 1233 2493 482 21 355
Fragments arranged by size:
2493 1233 482 355 21
AvaI C'yCGr_G
Cuts at: 0 2487 2710 3859 3928 4271 4584
Size: 2487 223 1149 69 343 313
Fragments arranged by size:
2487 1149 343 313 223 69
BanI G'GyrC_C
Cuts at: 0 2990 3391 3932 4257 4584
Size: 2990 401 541 325 327
Fragments arranged by size:
2990 541 401 327 325
BanII G_rGCy'C
Cuts at: 0 2035 2090 2653 2930 3022 3335 4584
Size: 2035 55 563 277 92 313 1249
Fragments arranged by size:
2035 1249 563 313 277 92 55
BbsI GAAGACnn'nnnn_
Cuts at: 0 1232 1766 4584
Size: 1232 534 2818
Fragments arranged by size:
2818 1232 534
BbvCI CC'TCA_GC
Cuts at: 0 1117 1753 1789 4584
Size: 1117 636 36 2795
Fragments arranged by size:
2795 1117 636 36
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1819 1853 1957 1991 3442 3476 4584
Size: 1819 34 104 34 1451 34 1108
Fragments arranged by size:
1819 1451 1108 104 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 627 4584
Size: 627 3957
BclI T'GATC_A
Cuts at: 0 1095 2075 4584
Size: 1095 980 2509
Fragments arranged by size:
2509 1095 980
BglI GCCn_nnn'nGGC
Cuts at: 0 1489 1736 3559 4584
Size: 1489 247 1823 1025
Fragments arranged by size:
1823 1489 1025 247
BglII A'GATC_T
Cuts at: 0 2144 3465 3515 4584
Size: 2144 1321 50 1069
Fragments arranged by size:
2144 1321 1069 50
BlpI GC'TnA_GC
Cuts at: 0 695 3684 4055 4584
Size: 695 2989 371 529
Fragments arranged by size:
2989 695 529 371
Bme1580I G_kGCm'C
Cuts at: 0 2090 3176 3533 3935 4329 4584
Size: 2090 1086 357 402 394 255
Fragments arranged by size:
2090 1086 402 394 357 255
BmgBI CAC'GTC
Cuts at: 0 3435 4584
Size: 3435 1149
BmrI ACTGGGnnnn_n'
Cuts at: 0 1392 4584
Size: 1392 3192
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 3747 4584
Size: 3747 837
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 592 901 1262 1863 2532 2649 2710 3148
Size: 592 309 361 601 669 117 61 438
Cuts at: 3148 3958 4317 4584
Size: 810 359 267
Fragments arranged by size:
810 669 601 592 438 361 359 309
267 117 61
Bpu10I CC'TnA_GC
Cuts at: 0 1117 1753 1789 2626 3473 3622 4584
Size: 1117 636 36 837 847 149 962
Fragments arranged by size:
1117 962 847 837 636 149 36
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2011 3794 4584
Size: 2011 1783 790
Fragments arranged by size:
2011 1783 790
BsaI GGTCTCn'nnnn_
Cuts at: 0 961 2236 2823 4584
Size: 961 1275 587 1761
Fragments arranged by size:
1761 1275 961 587
BsaAI yAC'GTr
Cuts at: 0 2736 4584
Size: 2736 1848
BsaBI GATnn'nnATC
Cuts at: 0 4352 4584
Size: 4352 232
BsaHI Gr'CG_yC
Cuts at: 0 1503 4584
Size: 1503 3081
BsaWI w'CCGG_w
Cuts at: 0 2919 3215 3305 4584
Size: 2919 296 90 1279
Fragments arranged by size:
2919 1279 296 90
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2280 2310 3956 3986 4584
Size: 2280 30 1646 30 598
Fragments arranged by size:
2280 1646 598 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1119 1866 2128 2208 4584
Size: 1119 747 262 80 2376
Fragments arranged by size:
2376 1119 747 262 80
BseYI C'CCAG_C
Cuts at: 0 26 1726 1810 1825 2067 2266 2881 3164
Size: 26 1700 84 15 242 199 615 283
Cuts at: 3164 4025 4584
Size: 861 559
Fragments arranged by size:
1700 861 615 559 283 242 199 84
26 15
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 3271 4470 4532 4584
Size: 3271 1199 62 52
Fragments arranged by size:
3271 1199 62 52
BsiEI CG_ry'CG
Cuts at: 0 2871 4356 4584
Size: 2871 1485 228
Fragments arranged by size:
2871 1485 228
BsiHKAI G_wGCw'C
Cuts at: 0 2035 3022 4391 4584
Size: 2035 987 1369 193
Fragments arranged by size:
2035 1369 987 193
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2586 4584
Size: 2586 1998
Bsp1286I G_dGCh'C
Cuts at: 0 2035 2090 2653 2930 3022 3176 3335 3533
Size: 2035 55 563 277 92 154 159 198
Cuts at: 3533 3935 4329 4391 4584
Size: 402 394 62 193
Fragments arranged by size:
2035 563 402 394 277 198 193 159
154 92 62 55
BspEI T'CCGG_A
Cuts at: 0 3305 4584
Size: 3305 1279
BspHI T'CATG_A
Cuts at: 0 3182 4584
Size: 3182 1402
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 754 1163 3095 4584
Size: 754 409 1932 1489
Fragments arranged by size:
1932 1489 754 409
BsrDI GCAATG_nn'
Cuts at: 0 588 756 1902 4584
Size: 588 168 1146 2682
Fragments arranged by size:
2682 1146 588 168
BssSI C'ACGA_G
Cuts at: 0 1226 4584
Size: 1226 3358
BstAPI GCAn_nnn'nTGC
Cuts at: 0 3294 3609 4584
Size: 3294 315 975
Fragments arranged by size:
3294 975 315
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1887 2536 2889 4584
Size: 1887 649 353 1695
Fragments arranged by size:
1887 1695 649 353
BstYI r'GATC_y
Cuts at: 0 1186 2144 2685 2936 3465 3515 4529 4584
Size: 1186 958 541 251 529 50 1014 55
Fragments arranged by size:
1186 1014 958 541 529 251 55 50
Bsu36I CC'TnA_GG
Cuts at: 0 2551 4584
Size: 2551 2033
BtgI C'CryG_G
Cuts at: 0 1061 1256 2180 2529 4584
Size: 1061 195 924 349 2055
Fragments arranged by size:
2055 1061 924 349 195
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 3641 4584
Size: 3641 943
BtsI GCAGTG_nn'
Cuts at: 0 448 635 955 4584
Size: 448 187 320 3629
Fragments arranged by size:
3629 448 320 187
ClaI AT'CG_AT
Cuts at: 0 1436 4584
Size: 1436 3148
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 233 268 4584
Size: 233 35 4316
Fragments arranged by size:
4316 233 35
DraI TTT'AAA
Cuts at: 0 383 1517 3999 4020 4584
Size: 383 1134 2482 21 564
Fragments arranged by size:
2482 1134 564 383 21
EaeI y'GGCC_r
Cuts at: 0 974 2538 3028 3674 4584
Size: 974 1564 490 646 910
Fragments arranged by size:
1564 974 910 646 490
EarI CTCTTCn'nnn_
Cuts at: 0 880 1646 2584 2918 3181 3340 3703 3712
Size: 880 766 938 334 263 159 363 9
Cuts at: 3712 4584
Size: 872
Fragments arranged by size:
938 880 872 766 363 334 263 159
9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2139 2698 4584
Size: 2139 559 1886
Fragments arranged by size:
2139 1886 559
EcoICRI GAG'CTC
Cuts at: 0 2033 3020 4584
Size: 2033 987 1564
Fragments arranged by size:
2033 1564 987
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 592 901 1262 1863 1929 1935 2041 2143
Size: 592 309 361 601 66 6 106 102
Cuts at: 2143 2450 2532 2608 2649 2710 3148 3321 3469
Size: 307 82 76 41 61 438 173 148
Cuts at: 3469 3736 3925 3958 4317 4527 4538 4584
Size: 267 189 33 359 210 11 46
Fragments arranged by size:
601 592 438 361 359 309 307 267
210 189 173 148 106 102 82 76
66 61 46 41 33 11 6
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1714 3735 4584
Size: 1714 2021 849
Fragments arranged by size:
2021 1714 849
EcoO109I rG'GnC_Cy
Cuts at: 0 1694 2282 2676 3231 4084 4584
Size: 1694 588 394 555 853 500
Fragments arranged by size:
1694 853 588 555 500 394
EcoRI G'AATT_C
Cuts at: 0 3726 4584
Size: 3726 858
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1024 4584
Size: 1024 3560
HaeII r_GCGC'y
Cuts at: 0 1764 2059 3546 3691 4152 4584
Size: 1764 295 1487 145 461 432
Fragments arranged by size:
1764 1487 461 432 295 145
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1165 1197 1237 1252 1269 1284 1980 2012
Size: 1165 32 40 15 17 15 696 32
Cuts at: 2012 2022 2054 2745 2777 2785 2803 2817 2835
Size: 10 32 691 32 8 18 14 18
Cuts at: 2835 3009 3041 3354 3386 3861 3893 4561 4584
Size: 174 32 313 32 475 32 668 23
Fragments arranged by size:
1165 696 691 668 475 313 174 40
32 32 32 32 32 32 32 23
18 18 17 15 15 14 10 8
HincII GTy'rAC
Cuts at: 0 2354 3246 3408 4458 4584
Size: 2354 892 162 1050 126
Fragments arranged by size:
2354 1050 892 162 126
HindIII A'AGCT_T
Cuts at: 0 591 1364 2523 3844 4584
Size: 591 773 1159 1321 740
Fragments arranged by size:
1321 1159 773 740 591
MfeI C'AATT_G
Cuts at: 0 670 4584
Size: 670 3914
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2219 2228 2750 3008 4169 4584
Size: 2219 9 522 258 1161 415
Fragments arranged by size:
2219 1161 522 415 258 9
MscI TGG'CCA
Cuts at: 0 976 2540 4584
Size: 976 1564 2044
Fragments arranged by size:
2044 1564 976
MslI CAynn'nnrTG
Cuts at: 0 275 1744 2855 4444 4508 4584
Size: 275 1469 1111 1589 64 76
Fragments arranged by size:
1589 1469 1111 275 76 64
MspA1I CmG'CkG
Cuts at: 0 868 2084 2751 3955 4516 4584
Size: 868 1216 667 1204 561 68
Fragments arranged by size:
1216 1204 868 667 561 68
NcoI C'CATG_G
Cuts at: 0 1061 2180 4584
Size: 1061 1119 2404
Fragments arranged by size:
2404 1119 1061
NdeI CA'TA_TG
Cuts at: 0 416 3824 4584
Size: 416 3408 760
Fragments arranged by size:
3408 760 416
NspI r_CATG'y
Cuts at: 0 40 92 328 948 2322 3447 4449 4476
Size: 40 52 236 620 1374 1125 1002 27
Cuts at: 4476 4584
Size: 108
Fragments arranged by size:
1374 1125 1002 620 236 108 52 40
27
PasI CC'CwG_GG
Cuts at: 0 1690 3737 4584
Size: 1690 2047 847
Fragments arranged by size:
2047 1690 847
PciI A'CATG_T
Cuts at: 0 36 4445 4584
Size: 36 4409 139
Fragments arranged by size:
4409 139 36
PflMI CCAn_nnn'nTGG
Cuts at: 0 51 3072 3318 4584
Size: 51 3021 246 1266
Fragments arranged by size:
3021 1266 246 51
PfoI T'CCnGG_A
Cuts at: 0 2939 3358 3974 4532 4584
Size: 2939 419 616 558 52
Fragments arranged by size:
2939 616 558 419 52
PmlI CAC'GTG
Cuts at: 0 2736 4584
Size: 2736 1848
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 3481 3513 4154 4186 4584
Size: 3481 32 641 32 398
Fragments arranged by size:
3481 641 398 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 1694 2282 2676 3231 4084 4584
Size: 1694 588 394 555 853 500
Fragments arranged by size:
1694 853 588 555 500 394
PshAI GACnn'nnGTC
Cuts at: 0 191 4584
Size: 191 4393
PspOMI G'GGCC_C
Cuts at: 0 2086 4584
Size: 2086 2498
PstI C_TGCA'G
Cuts at: 0 839 3095 3551 3605 4584
Size: 839 2256 456 54 979
Fragments arranged by size:
2256 979 839 456 54
PvuI CG_AT'CG
Cuts at: 0 4356 4584
Size: 4356 228
PvuII CAG'CTG
Cuts at: 0 868 3955 4516 4584
Size: 868 3087 561 68
Fragments arranged by size:
3087 868 561 68
SacI G_AGCT'C
Cuts at: 0 2035 3022 4584
Size: 2035 987 1562
Fragments arranged by size:
2035 1562 987
SanDI GG'GwC_CC
Cuts at: 0 1694 3231 4584
Size: 1694 1537 1353
Fragments arranged by size:
1694 1537 1353
SapI GCTCTTCn'nnn_
Cuts at: 0 2918 3340 4584
Size: 2918 422 1244
Fragments arranged by size:
2918 1244 422
SbfI CC_TGCA'GG
Cuts at: 0 3551 4584
Size: 3551 1033
SexAI A'CCwGG_T
Cuts at: 0 1682 2977 4584
Size: 1682 1295 1607
Fragments arranged by size:
1682 1607 1295
SfcI C'TryA_G
Cuts at: 0 5 835 3052 3091 3511 3547 3601 4584
Size: 5 830 2217 39 420 36 54 983
Fragments arranged by size:
2217 983 830 420 54 39 36 5
SmlI C'TyrA_G
Cuts at: 0 594 2026 3809 4271 4461 4584
Size: 594 1432 1783 462 190 123
Fragments arranged by size:
1783 1432 594 462 190 123
SpeI A'CTAG_T
Cuts at: 0 102 318 4584
Size: 102 216 4266
Fragments arranged by size:
4266 216 102
SphI G_CATG'C
Cuts at: 0 948 4584
Size: 948 3636
StyI C'CwwG_G
Cuts at: 0 390 423 1061 1358 1445 1928 2180 2579
Size: 390 33 638 297 87 483 252 399
Cuts at: 2579 3533 4584
Size: 954 1051
Fragments arranged by size:
1051 954 638 483 399 390 297 252
87 33
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 630 994 2805 2885 2895 4360 4584
Size: 630 364 1811 80 10 1465 224
Fragments arranged by size:
1811 1465 630 364 224 80 10
TatI w'GTAC_w
Cuts at: 0 756 2321 4584
Size: 756 1565 2263
Fragments arranged by size:
2263 1565 756
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1551 1583 2280 2312 4584
Size: 1551 32 697 32 2272
Fragments arranged by size:
2272 1551 697 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1684 2815 3403 4584
Size: 1684 1131 588 1181
Fragments arranged by size:
1684 1181 1131 588
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 3813 4584
Size: 3813 771
XhoI C'TCGA_G
Cuts at: 0 4271 4584
Size: 4271 313
XmnI GAAnn'nnTTC
Cuts at: 0 2952 3506 3847 4584
Size: 2952 554 341 737
Fragments arranged by size:
2952 737 554 341
Enzymes that do cut:
AarI AccI AcuI AflII AflIII AhdI AloI AlwNI
ApaI ApoI AvaI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI
Bsp1286I BspEI BspHI BspMI BsrDI BssSI BstAPI BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI HaeII Hin4I HincII HindIII MfeI MmeI MscI
MslI MspA1I NcoI NdeI NspI PasI PciI PflMI
PfoI PmlI PpiI PpuMI PshAI PspOMI PstI PvuI
PvuII SacI SanDI SapI SbfI SexAI SfcI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XcmI
XhoI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AgeI AleI AlfI ApaLI
AscI AseI AsiSI AvrII BaeI BamHI BbeI BfrBI
BmtI BsiWI BsmI BsrBI BsrFI BsrGI BssHII BstBI
BstEII BstZ17I DraIII DrdI EagI EcoRV FseI FspI
FspAI HpaI KasI KpnI MluI NaeI NarI NgoMIV
NheI NotI NruI NsiI PacI PmeI PsiI PspXI
PsrI RsrII SacII SalI ScaI SfiI SfoI SgfI
SgrAI SmaI SnaBI SrfI SspI StuI SwaI XbaI
XmaI ZraI