Restriction map for
pF1KSDA1730
(Linear) (Six-base) MAPSORT of: KIAA1730.seq Check: 9753 from: 1 to: 2232
KIAA1730.orf
With 182 enzymes: SgfI *
February 28, 2007 11:29 ..
AccI GT'mk_AC
Cuts at: 0 1596 2232
Size: 1596 636
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 456 560 1012 1727 2232
Size: 456 104 452 715 505
Fragments arranged by size:
715 505 456 452 104
AfeI AGC'GCT
Cuts at: 0 466 2232
Size: 466 1766
AflIII A'CryG_T
Cuts at: 0 2025 2232
Size: 2025 207
AhdI GACnn_n'nnGTC
Cuts at: 0 1856 2232
Size: 1856 376
AleI CACnn'nnGTG
Cuts at: 0 340 2232
Size: 340 1892
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 256 1160 1199 2232
Size: 256 904 39 1033
Fragments arranged by size:
1033 904 256 39
AlwNI CAG_nnn'CTG
Cuts at: 0 420 687 2232
Size: 420 267 1545
Fragments arranged by size:
1545 420 267
ApaLI G'TGCA_C
Cuts at: 0 1556 2232
Size: 1556 676
ApoI r'AATT_y
Cuts at: 0 69 429 922 1082 1125 1521 1543 1872
Size: 69 360 493 160 43 396 22 329
Cuts at: 1872 2232
Size: 360
Fragments arranged by size:
493 396 360 360 329 160 69 43
22
AseI AT'TA_AT
Cuts at: 0 1732 2232
Size: 1732 500
AvaI C'yCGr_G
Cuts at: 0 4 448 2232
Size: 4 444 1784
Fragments arranged by size:
1784 444 4
BamHI G'GATC_C
Cuts at: 0 398 453 1307 2232
Size: 398 55 854 925
Fragments arranged by size:
925 854 398 55
BanII G_rGCy'C
Cuts at: 0 1472 2232
Size: 1472 760
BbsI GAAGACnn'nnnn_
Cuts at: 0 536 2232
Size: 536 1696
BglII A'GATC_T
Cuts at: 0 275 2232
Size: 275 1957
Bme1580I G_kGCm'C
Cuts at: 0 1560 2232
Size: 1560 672
BmrI ACTGGGnnnn_n'
Cuts at: 0 1112 2232
Size: 1112 1120
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1892 2213 2232
Size: 1892 321 19
Fragments arranged by size:
1892 321 19
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 161 397 1904 2232
Size: 161 236 1507 328
Fragments arranged by size:
1507 328 236 161
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 109 616 2232
Size: 109 507 1616
Fragments arranged by size:
1616 507 109
BsaAI yAC'GTr
Cuts at: 0 834 2232
Size: 834 1398
BsaHI Gr'CG_yC
Cuts at: 0 37 2232
Size: 37 2195
BsaWI w'CCGG_w
Cuts at: 0 293 1304 2232
Size: 293 1011 928
Fragments arranged by size:
1011 928 293
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 22 52 2232
Size: 22 30 2180
Fragments arranged by size:
2180 30 22
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 46 2232
Size: 46 2186
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1436 2232
Size: 1436 796
BsiHKAI G_wGCw'C
Cuts at: 0 1472 1560 2232
Size: 1472 88 672
Fragments arranged by size:
1472 672 88
BsmI GAATG_Cn'
Cuts at: 0 260 351 1012 1893 2232
Size: 260 91 661 881 339
Fragments arranged by size:
881 661 339 260 91
BsmBI CGTCTCn'nnnn_
Cuts at: 0 44 2232
Size: 44 2188
Bsp1286I G_dGCh'C
Cuts at: 0 1472 1560 2232
Size: 1472 88 672
Fragments arranged by size:
1472 672 88
BspEI T'CCGG_A
Cuts at: 0 293 2232
Size: 293 1939
BspHI T'CATG_A
Cuts at: 0 1631 2109 2232
Size: 1631 478 123
Fragments arranged by size:
1631 478 123
BsrDI GCAATG_nn'
Cuts at: 0 550 2232
Size: 550 1682
BsrFI r'CCGG_y
Cuts at: 0 16 476 2232
Size: 16 460 1756
Fragments arranged by size:
1756 460 16
BstYI r'GATC_y
Cuts at: 0 275 398 453 1179 1307 2232
Size: 275 123 55 726 128 925
Fragments arranged by size:
925 726 275 128 123 55
BtgI C'CryG_G
Cuts at: 0 26 182 1241 2184 2232
Size: 26 156 1059 943 48
Fragments arranged by size:
1059 943 156 48 26
BtsI GCAGTG_nn'
Cuts at: 0 602 1305 1366 2232
Size: 602 703 61 866
Fragments arranged by size:
866 703 602 61
DraI TTT'AAA
Cuts at: 0 665 1088 2232
Size: 665 423 1144
Fragments arranged by size:
1144 665 423
DraIII CAC_nnn'GTG
Cuts at: 0 1779 2178 2232
Size: 1779 399 54
Fragments arranged by size:
1779 399 54
EaeI y'GGCC_r
Cuts at: 0 1912 2147 2232
Size: 1912 235 85
Fragments arranged by size:
1912 235 85
EarI CTCTTCn'nnn_
Cuts at: 0 770 1477 1633 2232
Size: 770 707 156 599
Fragments arranged by size:
770 707 599 156
EcoICRI GAG'CTC
Cuts at: 0 1470 2232
Size: 1470 762
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 161 397 456 560 1012 1727 1904 2232
Size: 161 236 59 104 452 715 177 328
Fragments arranged by size:
715 452 328 236 177 161 104 59
EcoO109I rG'GnC_Cy
Cuts at: 0 1946 2232
Size: 1946 286
EcoRI G'AATT_C
Cuts at: 0 429 922 1872 2232
Size: 429 493 950 360
Fragments arranged by size:
950 493 429 360
EcoRV GAT'ATC
Cuts at: 0 60 354 729 2232
Size: 60 294 375 1503
Fragments arranged by size:
1503 375 294 60
HaeII r_GCGC'y
Cuts at: 0 468 1154 2232
Size: 468 686 1078
Fragments arranged by size:
1078 686 468
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 22 54 1842 1874 2232
Size: 22 32 1788 32 358
Fragments arranged by size:
1788 358 32 32 22
HindIII A'AGCT_T
Cuts at: 0 299 1090 1348 1432 1844 2232
Size: 299 791 258 84 412 388
Fragments arranged by size:
791 412 388 299 258 84
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1227 1609 2157 2232
Size: 1227 382 548 75
Fragments arranged by size:
1227 548 382 75
MscI TGG'CCA
Cuts at: 0 1914 2149 2232
Size: 1914 235 83
Fragments arranged by size:
1914 235 83
MslI CAynn'nnrTG
Cuts at: 0 340 2232
Size: 340 1892
MspA1I CmG'CkG
Cuts at: 0 1807 2232
Size: 1807 425
NaeI GCC'GGC
Cuts at: 0 18 478 2232
Size: 18 460 1754
Fragments arranged by size:
1754 460 18
NcoI C'CATG_G
Cuts at: 0 26 182 1241 2184 2232
Size: 26 156 1059 943 48
Fragments arranged by size:
1059 943 156 48 26
NgoMIV G'CCGG_C
Cuts at: 0 16 476 2232
Size: 16 460 1756
Fragments arranged by size:
1756 460 16
NspI r_CATG'y
Cuts at: 0 882 1392 2029 2064 2232
Size: 882 510 637 35 168
Fragments arranged by size:
882 637 510 168 35
PciI A'CATG_T
Cuts at: 0 2025 2232
Size: 2025 207
PflMI CCAn_nnn'nTGG
Cuts at: 0 420 687 1972 2232
Size: 420 267 1285 260
Fragments arranged by size:
1285 420 267 260
PmlI CAC'GTG
Cuts at: 0 834 2232
Size: 834 1398
PpuMI rG'GwC_Cy
Cuts at: 0 1946 2232
Size: 1946 286
PshAI GACnn'nnGTC
Cuts at: 0 633 2232
Size: 633 1599
PspXI vC'TCGA_Gb
Cuts at: 0 448 2232
Size: 448 1784
PstI C_TGCA'G
Cuts at: 0 134 1191 2035 2232
Size: 134 1057 844 197
Fragments arranged by size:
1057 844 197 134
PvuII CAG'CTG
Cuts at: 0 1807 2232
Size: 1807 425
SacI G_AGCT'C
Cuts at: 0 1472 2232
Size: 1472 760
SapI GCTCTTCn'nnn_
Cuts at: 0 770 1477 2232
Size: 770 707 755
Fragments arranged by size:
770 755 707
SfcI C'TryA_G
Cuts at: 0 130 1187 1439 2031 2232
Size: 130 1057 252 592 201
Fragments arranged by size:
1057 592 252 201 130
SmlI C'TyrA_G
Cuts at: 0 88 448 631 2232
Size: 88 360 183 1601
Fragments arranged by size:
1601 360 183 88
SphI G_CATG'C
Cuts at: 0 882 2064 2232
Size: 882 1182 168
Fragments arranged by size:
1182 882 168
StyI C'CwwG_G
Cuts at: 0 26 182 1241 1614 2184 2232
Size: 26 156 1059 373 570 48
Fragments arranged by size:
1059 570 373 156 48 26
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 641 2232
Size: 641 1591
TatI w'GTAC_w
Cuts at: 0 1710 2232
Size: 1710 522
XbaI T'CTAG_A
Cuts at: 0 1641 2232
Size: 1641 591
XhoI C'TCGA_G
Cuts at: 0 448 2232
Size: 448 1784
XmnI GAAnn'nnTTC
Cuts at: 0 301 1250 2232
Size: 301 949 982
Fragments arranged by size:
982 949 301
Enzymes that do cut:
AccI AcuI AfeI AflIII AhdI AleI AlfI AlwNI
ApaLI ApoI AseI AvaI BamHI BanII BbsI BglII
Bme1580I BmrI BplI BpmI BpuEI BsaAI BsaHI BsaWI
BsaXI BseRI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BsrDI BsrFI BstYI BtgI BtsI DraI DraIII
EaeI EarI EcoICRI Eco57MI EcoO109I EcoRI EcoRV HaeII
Hin4I HindIII MmeI MscI MslI MspA1I NaeI NcoI
NgoMIV NspI PciI PflMI PmlI PpuMI PshAI PspXI
PstI PvuII SacI SapI SfcI SmlI SphI StyI
TaqII TatI XbaI XhoI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AflII AgeI AloI ApaI
AscI AsiSI AvrII BaeI BanI BbeI BbvCI BcgI
BciVI BclI BfrBI BglI BlpI BmgBI BmtI Bpu10I
BsaI BsaBI BseYI BsiEI BsiWI BspMI BsrBI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I
BtgZI ClaI CspCI DrdI EagI EciI EcoNI FalI
FseI FspI FspAI HincII HpaI KasI KpnI MfeI
MluI NarI NdeI NheI NotI NruI NsiI PacI
PasI PfoI PmeI PpiI PsiI PspOMI PsrI PvuI
RsrII SacII SalI SanDI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI
StuI SwaI TstI Tth111I XcmI XmaI ZraI