Restriction map for
pF1KSDA1733
(Linear) (Six-base) MAPSORT of: KIAA1733.seq Check: 1882 from: 1 to: 1452
KIAA1733.orf
With 182 enzymes: SgfI *
October 24, 2006 15:17 ..
AccI GT'mk_AC
Cuts at: 0 1174 1452
Size: 1174 278
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 496 1452
Size: 496 956
AflIII A'CryG_T
Cuts at: 0 404 1452
Size: 404 1048
AleI CACnn'nnGTG
Cuts at: 0 1040 1452
Size: 1040 412
AlwNI CAG_nnn'CTG
Cuts at: 0 549 667 978 1452
Size: 549 118 311 474
Fragments arranged by size:
549 474 311 118
AseI AT'TA_AT
Cuts at: 0 136 1452
Size: 136 1316
AvaI C'yCGr_G
Cuts at: 0 921 1038 1452
Size: 921 117 414
Fragments arranged by size:
921 414 117
BamHI G'GATC_C
Cuts at: 0 612 1452
Size: 612 840
BanI G'GyrC_C
Cuts at: 0 301 670 1375 1452
Size: 301 369 705 77
Fragments arranged by size:
705 369 301 77
BanII G_rGCy'C
Cuts at: 0 99 556 947 1452
Size: 99 457 391 505
Fragments arranged by size:
505 457 391 99
BbeI G_GCGC'C
Cuts at: 0 674 1452
Size: 674 778
BbsI GAAGACnn'nnnn_
Cuts at: 0 1163 1214 1452
Size: 1163 51 238
Fragments arranged by size:
1163 238 51
BbvCI CC'TCA_GC
Cuts at: 0 251 1360 1452
Size: 251 1109 92
Fragments arranged by size:
1109 251 92
BlpI GC'TnA_GC
Cuts at: 0 1396 1452
Size: 1396 56
BmgBI CAC'GTC
Cuts at: 0 407 1452
Size: 407 1045
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 540 1375 1452
Size: 540 835 77
Fragments arranged by size:
835 540 77
Bpu10I CC'TnA_GC
Cuts at: 0 251 550 1269 1360 1452
Size: 251 299 719 91 92
Fragments arranged by size:
719 299 251 92 91
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 83 1452
Size: 83 1369
BsaHI Gr'CG_yC
Cuts at: 0 671 1452
Size: 671 781
BsaWI w'CCGG_w
Cuts at: 0 179 1452
Size: 179 1273
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 874 904 1219 1249 1279 1309 1452
Size: 874 30 315 30 30 30 143
Fragments arranged by size:
874 315 143 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 482 1201 1210 1213 1360 1452
Size: 482 719 9 3 147 92
Fragments arranged by size:
719 482 147 92 9 3
BseYI C'CCAG_C
Cuts at: 0 815 1452
Size: 815 637
BsiHKAI G_wGCw'C
Cuts at: 0 99 556 635 947 1452
Size: 99 457 79 312 505
Fragments arranged by size:
505 457 312 99 79
Bsp1286I G_dGCh'C
Cuts at: 0 99 556 635 947 1452
Size: 99 457 79 312 505
Fragments arranged by size:
505 457 312 99 79
BspEI T'CCGG_A
Cuts at: 0 179 1452
Size: 179 1273
BspHI T'CATG_A
Cuts at: 0 734 1140 1452
Size: 734 406 312
Fragments arranged by size:
734 406 312
BsrBI CCG'CTC
Cuts at: 0 1350 1452
Size: 1350 102
BsrFI r'CCGG_y
Cuts at: 0 167 898 1452
Size: 167 731 554
Fragments arranged by size:
731 554 167
BstAPI GCAn_nnn'nTGC
Cuts at: 0 858 1452
Size: 858 594
BstEII G'GTnAC_C
Cuts at: 0 1050 1452
Size: 1050 402
BstXI CCAn_nnnn'nTGG
Cuts at: 0 138 796 1027 1239 1452
Size: 138 658 231 212 213
Fragments arranged by size:
658 231 213 212 138
BstYI r'GATC_y
Cuts at: 0 227 612 663 845 1452
Size: 227 385 51 182 607
Fragments arranged by size:
607 385 227 182 51
BstZ17I GTA'TAC
Cuts at: 0 1175 1452
Size: 1175 277
BtgI C'CryG_G
Cuts at: 0 749 873 1452
Size: 749 124 579
Fragments arranged by size:
749 579 124
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 289 1452
Size: 289 1163
DrdI GACnn_nn'nnGTC
Cuts at: 0 292 1452
Size: 292 1160
EaeI y'GGCC_r
Cuts at: 0 798 876 959 1241 1452
Size: 798 78 83 282 211
Fragments arranged by size:
798 282 211 83 78
EarI CTCTTCn'nnn_
Cuts at: 0 446 515 787 1167 1178 1187 1452
Size: 446 69 272 380 11 9 265
Fragments arranged by size:
446 380 272 265 69 11 9
EcoICRI GAG'CTC
Cuts at: 0 97 554 945 1452
Size: 97 457 391 507
Fragments arranged by size:
507 457 391 97
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 496 540 1375 1452
Size: 496 44 835 77
Fragments arranged by size:
835 496 77 44
EcoNI CCTnn'n_nnAGG
Cuts at: 0 217 1452
Size: 217 1235
EcoO109I rG'GnC_Cy
Cuts at: 0 999 1063 1292 1452
Size: 999 64 229 160
Fragments arranged by size:
999 229 160 64
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1284 1452
Size: 1284 168
HaeII r_GCGC'y
Cuts at: 0 674 1452
Size: 674 778
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 22 49 54 81 604 636 1204 1236
Size: 22 27 5 27 523 32 568 32
Cuts at: 1236 1452
Size: 216
Fragments arranged by size:
568 523 216 32 32 27 27 22
5
KasI G'GCGC_C
Cuts at: 0 670 1452
Size: 670 782
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 660 1452
Size: 660 792
MscI TGG'CCA
Cuts at: 0 800 961 1243 1452
Size: 800 161 282 209
Fragments arranged by size:
800 282 209 161
MslI CAynn'nnrTG
Cuts at: 0 136 967 1040 1452
Size: 136 831 73 412
Fragments arranged by size:
831 412 136 73
MspA1I CmG'CkG
Cuts at: 0 250 564 858 996 1452
Size: 250 314 294 138 456
Fragments arranged by size:
456 314 294 250 138
NarI GG'CG_CC
Cuts at: 0 671 1452
Size: 671 781
NdeI CA'TA_TG
Cuts at: 0 624 1452
Size: 624 828
PasI CC'CwG_GG
Cuts at: 0 608 1452
Size: 608 844
PflMI CCAn_nnn'nTGG
Cuts at: 0 667 1452
Size: 667 785
PpuMI rG'GwC_Cy
Cuts at: 0 999 1063 1452
Size: 999 64 389
Fragments arranged by size:
999 389 64
PstI C_TGCA'G
Cuts at: 0 863 931 1258 1416 1452
Size: 863 68 327 158 36
Fragments arranged by size:
863 327 158 68 36
PvuII CAG'CTG
Cuts at: 0 250 564 858 1452
Size: 250 314 294 594
Fragments arranged by size:
594 314 294 250
SacI G_AGCT'C
Cuts at: 0 99 556 947 1452
Size: 99 457 391 505
Fragments arranged by size:
505 457 391 99
SanDI GG'GwC_CC
Cuts at: 0 999 1452
Size: 999 453
SapI GCTCTTCn'nnn_
Cuts at: 0 446 1452
Size: 446 1006
SfcI C'TryA_G
Cuts at: 0 84 859 927 1254 1412 1452
Size: 84 775 68 327 158 40
Fragments arranged by size:
775 327 158 84 68 40
SfoI GGC'GCC
Cuts at: 0 672 1452
Size: 672 780
SmlI C'TyrA_G
Cuts at: 0 98 1452
Size: 98 1354
StyI C'CwwG_G
Cuts at: 0 364 1452
Size: 364 1088
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 319 330 1452
Size: 319 11 1122
Fragments arranged by size:
1122 319 11
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 874 906 1452
Size: 874 32 546
Fragments arranged by size:
874 546 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 975 1305 1452
Size: 975 330 147
Fragments arranged by size:
975 330 147
Enzymes that do cut:
AccI AcuI AflIII AleI AlwNI AseI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BlpI BmgBI BpmI
Bpu10I BpuEI BsaHI BsaWI BsaXI BseRI BseYI BsiHKAI
Bsp1286I BspEI BspHI BsrBI BsrFI BstAPI BstEII BstXI
BstYI BstZ17I BtgI BtgZI DrdI EaeI EarI EcoICRI
Eco57MI EcoNI EcoO109I FalI HaeII Hin4I KasI MmeI
MscI MslI MspA1I NarI NdeI PasI PflMI PpuMI
PstI PvuII SacI SanDI SapI SfcI SfoI SmlI
StyI TaqII TstI XcmI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AlfI AloI ApaI ApaLI ApoI AscI AsiSI AvrII
BaeI BcgI BciVI BclI BfrBI BglI BglII Bme1580I
BmrI BmtI BplI BsaI BsaAI BsaBI BsgI BsiEI
BsiWI BsmI BsmBI BspMI BsrDI BsrGI BssHII BssSI
BstBI Bsu36I BtsI ClaI CspCI DraI DraIII EagI
EciI EcoRI EcoRV FseI FspI FspAI HincII HindIII
HpaI KpnI MfeI MluI NaeI NcoI NgoMIV NheI
NotI NruI NsiI NspI PacI PciI PfoI PmeI
PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI
RsrII SacII SalI SbfI ScaI SexAI SfiI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI
SwaI TatI Tth111I XbaI XhoI XmaI XmnI ZraI