Restriction map for
pF1KSDA1735
(Linear) (Six-base) MAPSORT of: KIAA1735.seq Check: 4683 from: 1 to: 1416
KIAA1735.orf
With 182 enzymes: SgfI *
October 24, 2006 15:17 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 134 332 508 652 860 930 1416
Size: 134 198 176 144 208 70 486
Fragments arranged by size:
486 208 198 176 144 134 70
AgeI A'CCGG_T
Cuts at: 0 1190 1286 1416
Size: 1190 96 130
Fragments arranged by size:
1190 130 96
AlwNI CAG_nnn'CTG
Cuts at: 0 1163 1416
Size: 1163 253
AvaI C'yCGr_G
Cuts at: 0 158 361 1416
Size: 158 203 1055
Fragments arranged by size:
1055 203 158
BamHI G'GATC_C
Cuts at: 0 301 1308 1416
Size: 301 1007 108
Fragments arranged by size:
1007 301 108
BanII G_rGCy'C
Cuts at: 0 181 1144 1416
Size: 181 963 272
Fragments arranged by size:
963 272 181
BbsI GAAGACnn'nnnn_
Cuts at: 0 1406 1416
Size: 1406 10
BbvCI CC'TCA_GC
Cuts at: 0 962 1416
Size: 962 454
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 640 674 895 929 1416
Size: 640 34 221 34 487
Fragments arranged by size:
640 487 221 34 34
BmrI ACTGGGnnnn_n'
Cuts at: 0 29 69 1416
Size: 29 40 1347
Fragments arranged by size:
1347 40 29
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 518 940 1416
Size: 518 422 476
Fragments arranged by size:
518 476 422
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 214 1416
Size: 214 1202
Bpu10I CC'TnA_GC
Cuts at: 0 962 1416
Size: 962 454
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1064 1416
Size: 1064 352
BsaI GGTCTCn'nnnn_
Cuts at: 0 190 1416
Size: 190 1226
BsaBI GATnn'nnATC
Cuts at: 0 379 1416
Size: 379 1037
BsaWI w'CCGG_w
Cuts at: 0 701 1190 1286 1416
Size: 701 489 96 130
Fragments arranged by size:
701 489 130 96
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 132 162 1228 1258 1416
Size: 132 30 1066 30 158
Fragments arranged by size:
1066 158 132 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 424 1249 1348 1416
Size: 424 825 99 68
Fragments arranged by size:
825 424 99 68
BseYI C'CCAG_C
Cuts at: 0 645 1416
Size: 645 771
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 556 1416
Size: 556 860
BsiHKAI G_wGCw'C
Cuts at: 0 1144 1178 1416
Size: 1144 34 238
Fragments arranged by size:
1144 238 34
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1024 1416
Size: 1024 392
Bsp1286I G_dGCh'C
Cuts at: 0 181 1144 1178 1416
Size: 181 963 34 238
Fragments arranged by size:
963 238 181 34
BspEI T'CCGG_A
Cuts at: 0 701 1416
Size: 701 715
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 911 1416
Size: 911 505
BsrDI GCAATG_nn'
Cuts at: 0 843 1416
Size: 843 573
BsrFI r'CCGG_y
Cuts at: 0 617 1190 1286 1416
Size: 617 573 96 130
Fragments arranged by size:
617 573 130 96
BstYI r'GATC_y
Cuts at: 0 301 588 735 1308 1416
Size: 301 287 147 573 108
Fragments arranged by size:
573 301 287 147 108
BtgI C'CryG_G
Cuts at: 0 1404 1416
Size: 1404 12
DraI TTT'AAA
Cuts at: 0 1257 1416
Size: 1257 159
DraIII CAC_nnn'GTG
Cuts at: 0 732 1416
Size: 732 684
EaeI y'GGCC_r
Cuts at: 0 169 951 1416
Size: 169 782 465
Fragments arranged by size:
782 465 169
EarI CTCTTCn'nnn_
Cuts at: 0 77 1416
Size: 77 1339
EcoICRI GAG'CTC
Cuts at: 0 1142 1416
Size: 1142 274
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 134 214 332 508 652 860 930 1416
Size: 134 80 118 176 144 208 70 486
Fragments arranged by size:
486 208 176 144 134 118 80 70
EcoO109I rG'GnC_Cy
Cuts at: 0 329 1416
Size: 329 1087
EcoRV GAT'ATC
Cuts at: 0 272 1416
Size: 272 1144
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 501 1416
Size: 501 915
FspI TGC'GCA
Cuts at: 0 937 1416
Size: 937 479
HaeII r_GCGC'y
Cuts at: 0 1017 1416
Size: 1017 399
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 132 164 1416
Size: 132 32 1252
Fragments arranged by size:
1252 132 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 200 877 1171 1211 1416
Size: 200 677 294 40 205
Fragments arranged by size:
677 294 205 200 40
MscI TGG'CCA
Cuts at: 0 953 1416
Size: 953 463
MslI CAynn'nnrTG
Cuts at: 0 761 1349 1416
Size: 761 588 67
Fragments arranged by size:
761 588 67
MspA1I CmG'CkG
Cuts at: 0 112 216 1416
Size: 112 104 1200
Fragments arranged by size:
1200 112 104
NcoI C'CATG_G
Cuts at: 0 1404 1416
Size: 1404 12
NspI r_CATG'y
Cuts at: 0 1024 1416
Size: 1024 392
PflMI CCAn_nnn'nTGG
Cuts at: 0 585 732 1416
Size: 585 147 684
Fragments arranged by size:
684 585 147
PshAI GACnn'nnGTC
Cuts at: 0 13 1416
Size: 13 1403
PsiI TTA'TAA
Cuts at: 0 70 379 1416
Size: 70 309 1037
Fragments arranged by size:
1037 309 70
PstI C_TGCA'G
Cuts at: 0 596 908 1416
Size: 596 312 508
Fragments arranged by size:
596 508 312
PvuII CAG'CTG
Cuts at: 0 216 1416
Size: 216 1200
SacI G_AGCT'C
Cuts at: 0 1144 1416
Size: 1144 272
SexAI A'CCwGG_T
Cuts at: 0 348 728 1416
Size: 348 380 688
Fragments arranged by size:
688 380 348
SfcI C'TryA_G
Cuts at: 0 280 592 904 1416
Size: 280 312 312 512
Fragments arranged by size:
512 312 312 280
SmlI C'TyrA_G
Cuts at: 0 158 1079 1416
Size: 158 921 337
Fragments arranged by size:
921 337 158
SspI AAT'ATT
Cuts at: 0 235 1416
Size: 235 1181
StuI AGG'CCT
Cuts at: 0 286 1416
Size: 286 1130
StyI C'CwwG_G
Cuts at: 0 332 1404 1416
Size: 332 1072 12
Fragments arranged by size:
1072 332 12
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 48 1086 1380 1416
Size: 48 1038 294 36
Fragments arranged by size:
1038 294 48 36
TatI w'GTAC_w
Cuts at: 0 1165 1416
Size: 1165 251
XbaI T'CTAG_A
Cuts at: 0 738 1416
Size: 738 678
XhoI C'TCGA_G
Cuts at: 0 158 1416
Size: 158 1258
Enzymes that do cut:
AcuI AgeI AlwNI AvaI BamHI BanII BbsI BbvCI
BcgI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaBI
BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I
BspEI BspMI BsrDI BsrFI BstYI BtgI DraI DraIII
EaeI EarI EcoICRI Eco57MI EcoO109I EcoRV FalI FspI
HaeII Hin4I MmeI MscI MslI MspA1I NcoI NspI
PflMI PshAI PsiI PstI PvuII SacI SexAI SfcI
SmlI SspI StuI StyI TaqII TatI XbaI XhoI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AhdI AleI AlfI AloI ApaI ApaLI ApoI AscI
AseI AsiSI AvrII BaeI BanI BbeI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmtI BsaAI
BsaHI BsiEI BsiWI BsmI BspHI BsrBI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI
BtsI ClaI CspCI DrdI EagI EciI EcoNI EcoRI
FseI FspAI HincII HindIII HpaI KasI KpnI MfeI
MluI NaeI NarI NdeI NgoMIV NheI NotI NruI
NsiI PacI PasI PciI PfoI PmeI PmlI PpiI
PpuMI PspOMI PspXI PsrI PvuI RsrII SacII SalI
SanDI SapI SbfI ScaI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SwaI TstI Tth111I
XcmI XmaI XmnI ZraI