Restriction map for
pF1KA1739
(Linear) (Six-base) MAPSORT of: KIAA1739.seq Check: 78 from: 1 to: 2499
KIAA1739.orf
With 182 enzymes: SgfI *
October 24, 2006 15:09 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 744 1197 2499
Size: 744 453 1302
Fragments arranged by size:
1302 744 453
AccI GT'mk_AC
Cuts at: 0 704 2499
Size: 704 1795
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 210 699 856 1622 1934 2499
Size: 210 489 157 766 312 565
Fragments arranged by size:
766 565 489 312 210 157
AflII C'TTAA_G
Cuts at: 0 2227 2499
Size: 2227 272
AflIII A'CryG_T
Cuts at: 0 968 2499
Size: 968 1531
AleI CACnn'nnGTG
Cuts at: 0 1699 2499
Size: 1699 800
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1462 2499
Size: 1462 1037
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 554 586 2499
Size: 554 32 1913
Fragments arranged by size:
1913 554 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1574 1833 2307 2466 2499
Size: 1574 259 474 159 33
Fragments arranged by size:
1574 474 259 159 33
ApaI G_GGCC'C
Cuts at: 0 590 1030 1367 1971 2273 2499
Size: 590 440 337 604 302 226
Fragments arranged by size:
604 590 440 337 302 226
ApaLI G'TGCA_C
Cuts at: 0 408 2499
Size: 408 2091
AvaI C'yCGr_G
Cuts at: 0 206 1535 1798 1964 2404 2482 2499
Size: 206 1329 263 166 440 78 17
Fragments arranged by size:
1329 440 263 206 166 78 17
BamHI G'GATC_C
Cuts at: 0 2193 2499
Size: 2193 306
BanI G'GyrC_C
Cuts at: 0 202 385 1127 1249 2252 2408 2499
Size: 202 183 742 122 1003 156 91
Fragments arranged by size:
1003 742 202 183 156 122 91
BanII G_rGCy'C
Cuts at: 0 182 590 1030 1367 1412 1971 2144 2273
Size: 182 408 440 337 45 559 173 129
Cuts at: 2273 2405 2426 2499
Size: 132 21 73
Fragments arranged by size:
559 440 408 337 182 173 132 129
73 45 21
BbeI G_GCGC'C
Cuts at: 0 206 1131 2499
Size: 206 925 1368
Fragments arranged by size:
1368 925 206
BbsI GAAGACnn'nnnn_
Cuts at: 0 92 292 610 2499
Size: 92 200 318 1889
Fragments arranged by size:
1889 318 200 92
BbvCI CC'TCA_GC
Cuts at: 0 62 2499
Size: 62 2437
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 727 761 1928 1962 2499
Size: 727 34 1167 34 537
Fragments arranged by size:
1167 727 537 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 307 2499
Size: 307 2192
BglI GCCn_nnn'nGGC
Cuts at: 0 186 211 503 2017 2499
Size: 186 25 292 1514 482
Fragments arranged by size:
1514 482 292 186 25
Bme1580I G_kGCm'C
Cuts at: 0 412 559 590 1030 1252 1367 1711 1971
Size: 412 147 31 440 222 115 344 260
Cuts at: 1971 2273 2411 2499
Size: 302 138 88
Fragments arranged by size:
440 412 344 302 260 222 147 138
115 88 31
BmrI ACTGGGnnnn_n'
Cuts at: 0 21 194 2499
Size: 21 173 2305
Fragments arranged by size:
2305 173 21
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 546 2499
Size: 546 1953
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 262 1171 1575 1810 2101 2146 2270 2499
Size: 262 909 404 235 291 45 124 229
Fragments arranged by size:
909 404 291 262 235 229 124 45
Bpu10I CC'TnA_GC
Cuts at: 0 62 491 2420 2499
Size: 62 429 1929 79
Fragments arranged by size:
1929 429 79 62
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1330 2499
Size: 1330 1169
BsaAI yAC'GTr
Cuts at: 0 198 2367 2499
Size: 198 2169 132
Fragments arranged by size:
2169 198 132
BsaHI Gr'CG_yC
Cuts at: 0 203 1128 2499
Size: 203 925 1371
Fragments arranged by size:
1371 925 203
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 349 379 1129 1159 1723 1753 2499
Size: 349 30 750 30 564 30 746
Fragments arranged by size:
750 746 564 349 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1216 1399 1728 1903 2499
Size: 1216 183 329 175 596
Fragments arranged by size:
1216 596 329 183 175
BseYI C'CCAG_C
Cuts at: 0 486 1087 1850 2499
Size: 486 601 763 649
Fragments arranged by size:
763 649 601 486
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1498 1538 2499
Size: 1498 40 961
Fragments arranged by size:
1498 961 40
BsiEI CG_ry'CG
Cuts at: 0 1278 2499
Size: 1278 1221
BsiHKAI G_wGCw'C
Cuts at: 0 412 2426 2499
Size: 412 2014 73
Fragments arranged by size:
2014 412 73
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1325 2433 2499
Size: 1325 1108 66
Fragments arranged by size:
1325 1108 66
Bsp1286I G_dGCh'C
Cuts at: 0 182 412 559 590 1030 1252 1367 1412
Size: 182 230 147 31 440 222 115 45
Cuts at: 1412 1711 1971 2144 2273 2405 2411 2426 2499
Size: 299 260 173 129 132 6 15 73
Fragments arranged by size:
440 299 260 230 222 182 173 147
132 129 115 73 45 31 15 6
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 744 1197 1790 2499
Size: 744 453 593 709
Fragments arranged by size:
744 709 593 453
BsrBI CCG'CTC
Cuts at: 0 723 1466 2499
Size: 723 743 1033
Fragments arranged by size:
1033 743 723
BsrDI GCAATG_nn'
Cuts at: 0 2053 2499
Size: 2053 446
BsrFI r'CCGG_y
Cuts at: 0 142 1456 2060 2499
Size: 142 1314 604 439
Fragments arranged by size:
1314 604 439 142
BssSI C'ACGA_G
Cuts at: 0 764 1104 1237 2499
Size: 764 340 133 1262
Fragments arranged by size:
1262 764 340 133
BstAPI GCAn_nnn'nTGC
Cuts at: 0 856 897 906 2415 2499
Size: 856 41 9 1509 84
Fragments arranged by size:
1509 856 84 41 9
BstEII G'GTnAC_C
Cuts at: 0 1950 2499
Size: 1950 549
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1149 2263 2499
Size: 1149 1114 236
Fragments arranged by size:
1149 1114 236
BstYI r'GATC_y
Cuts at: 0 1007 1600 2193 2499
Size: 1007 593 593 306
Fragments arranged by size:
1007 593 593 306
BtgI C'CryG_G
Cuts at: 0 116 275 1131 1487 1583 1656 2499
Size: 116 159 856 356 96 73 843
Fragments arranged by size:
856 843 356 159 116 96 73
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 910 2499
Size: 910 1589
BtsI GCAGTG_nn'
Cuts at: 0 734 1875 2464 2499
Size: 734 1141 589 35
Fragments arranged by size:
1141 734 589 35
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 921 940 956 975 1638 1673 2499
Size: 921 19 16 19 663 35 826
Fragments arranged by size:
921 826 663 35 19 19 16
DraIII CAC_nnn'GTG
Cuts at: 0 1269 2499
Size: 1269 1230
DrdI GACnn_nn'nnGTC
Cuts at: 0 700 2499
Size: 700 1799
EaeI y'GGCC_r
Cuts at: 0 113 533 980 1271 1490 1580 1590 2499
Size: 113 420 447 291 219 90 10 909
Fragments arranged by size:
909 447 420 291 219 113 90 10
EarI CTCTTCn'nnn_
Cuts at: 0 998 2069 2499
Size: 998 1071 430
Fragments arranged by size:
1071 998 430
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2358 2499
Size: 2358 141
EcoICRI GAG'CTC
Cuts at: 0 2424 2499
Size: 2424 75
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 210 262 699 856 1171 1575 1622 1810
Size: 210 52 437 157 315 404 47 188
Cuts at: 1810 1934 2101 2146 2270 2499
Size: 124 167 45 124 229
Fragments arranged by size:
437 404 315 229 210 188 167 157
124 124 52 47 45
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1381 2499
Size: 1381 1118
EcoO109I rG'GnC_Cy
Cuts at: 0 45 213 237 270 586 587 1026 1027
Size: 45 168 24 33 316 1 439 1
Cuts at: 1027 1213 1363 1364 1539 1779 1802 1830 1868
Size: 186 150 1 175 240 23 28 38
Cuts at: 1868 1968 2108 2269 2270 2499
Size: 100 140 161 1 229
Fragments arranged by size:
439 316 240 229 186 175 168 161
150 140 100 45 38 33 28 24
23 1 1 1 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 723 1467 1939 2499
Size: 723 744 472 560
Fragments arranged by size:
744 723 560 472
HaeII r_GCGC'y
Cuts at: 0 206 1131 2499
Size: 206 925 1368
Fragments arranged by size:
1368 925 206
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 2476 2499
Size: 2476 23
HincII GTy'rAC
Cuts at: 0 420 1563 2499
Size: 420 1143 936
Fragments arranged by size:
1143 936 420
KasI G'GCGC_C
Cuts at: 0 202 1127 2499
Size: 202 925 1372
Fragments arranged by size:
1372 925 202
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 761 980 1766 2341 2499
Size: 761 219 786 575 158
Fragments arranged by size:
786 761 575 219 158
MscI TGG'CCA
Cuts at: 0 115 1592 2499
Size: 115 1477 907
Fragments arranged by size:
1477 907 115
MslI CAynn'nnrTG
Cuts at: 0 1696 1699 2499
Size: 1696 3 800
Fragments arranged by size:
1696 800 3
MspA1I CmG'CkG
Cuts at: 0 888 1014 1194 1395 1473 1482 1574 1760
Size: 888 126 180 201 78 9 92 186
Cuts at: 1760 2499
Size: 739
Fragments arranged by size:
888 739 201 186 180 126 92 78
9
NaeI GCC'GGC
Cuts at: 0 1458 2062 2499
Size: 1458 604 437
Fragments arranged by size:
1458 604 437
NarI GG'CG_CC
Cuts at: 0 203 1128 2499
Size: 203 925 1371
Fragments arranged by size:
1371 925 203
NgoMIV G'CCGG_C
Cuts at: 0 1456 2060 2499
Size: 1456 604 439
Fragments arranged by size:
1456 604 439
NspI r_CATG'y
Cuts at: 0 426 854 972 1621 2248 2499
Size: 426 428 118 649 627 251
Fragments arranged by size:
649 627 428 426 251 118
PasI CC'CwG_GG
Cuts at: 0 1367 2013 2499
Size: 1367 646 486
Fragments arranged by size:
1367 646 486
PciI A'CATG_T
Cuts at: 0 968 2499
Size: 968 1531
PflMI CCAn_nnn'nTGG
Cuts at: 0 1269 1833 2499
Size: 1269 564 666
Fragments arranged by size:
1269 666 564
PfoI T'CCnGG_A
Cuts at: 0 149 2499
Size: 149 2350
PpuMI rG'GwC_Cy
Cuts at: 0 1539 1779 1802 2108 2499
Size: 1539 240 23 306 391
Fragments arranged by size:
1539 391 306 240 23
PspOMI G'GGCC_C
Cuts at: 0 586 1026 1363 1967 2269 2499
Size: 586 440 337 604 302 230
Fragments arranged by size:
604 586 440 337 302 230
PstI C_TGCA'G
Cuts at: 0 353 815 893 911 1394 2453 2499
Size: 353 462 78 18 483 1059 46
Fragments arranged by size:
1059 483 462 353 78 46 18
PvuII CAG'CTG
Cuts at: 0 888 1395 1473 1482 2499
Size: 888 507 78 9 1017
Fragments arranged by size:
1017 888 507 78 9
SacI G_AGCT'C
Cuts at: 0 2426 2499
Size: 2426 73
SanDI GG'GwC_CC
Cuts at: 0 1539 2499
Size: 1539 960
SapI GCTCTTCn'nnn_
Cuts at: 0 2069 2499
Size: 2069 430
SbfI CC_TGCA'GG
Cuts at: 0 911 2499
Size: 911 1588
SexAI A'CCwGG_T
Cuts at: 0 1265 1715 2499
Size: 1265 450 784
Fragments arranged by size:
1265 784 450
SfcI C'TryA_G
Cuts at: 0 349 811 889 907 1390 2449 2499
Size: 349 462 78 18 483 1059 50
Fragments arranged by size:
1059 483 462 349 78 50 18
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 503 2499
Size: 503 1996
SfoI GGC'GCC
Cuts at: 0 204 1129 2499
Size: 204 925 1370
Fragments arranged by size:
1370 925 204
SgrAI Cr'CCGG_yG
Cuts at: 0 2060 2499
Size: 2060 439
SmaI CCC'GGG
Cuts at: 0 1537 1800 1966 2499
Size: 1537 263 166 533
Fragments arranged by size:
1537 533 263 166
SmlI C'TyrA_G
Cuts at: 0 1345 2227 2499
Size: 1345 882 272
Fragments arranged by size:
1345 882 272
SphI G_CATG'C
Cuts at: 0 854 2248 2499
Size: 854 1394 251
Fragments arranged by size:
1394 854 251
SrfI GCCC'GGGC
Cuts at: 0 1966 2499
Size: 1966 533
StyI C'CwwG_G
Cuts at: 0 1955 2093 2111 2135 2499
Size: 1955 138 18 24 364
Fragments arranged by size:
1955 364 138 24 18
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 337 1276 1576 2499
Size: 337 939 300 923
Fragments arranged by size:
939 923 337 300
TatI w'GTAC_w
Cuts at: 0 527 2499
Size: 527 1972
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1723 1755 2499
Size: 1723 32 744
Fragments arranged by size:
1723 744 32
Tth111I GACn'n_nGTC
Cuts at: 0 96 700 1525 2499
Size: 96 604 825 974
Fragments arranged by size:
974 825 604 96
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1419 1575 2499
Size: 1419 156 924
Fragments arranged by size:
1419 924 156
XmaI C'CCGG_G
Cuts at: 0 1535 1798 1964 2499
Size: 1535 263 166 535
Fragments arranged by size:
1535 535 263 166
Enzymes that do cut:
AarI AccI AcuI AflII AflIII AleI AlfI AloI
AlwNI ApaI ApaLI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BglI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaAI BsaHI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstEII BstXI BstYI BtgI BtgZI
BtsI CspCI DraIII DrdI EaeI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I FalI HaeII Hin4I HincII KasI
MmeI MscI MslI MspA1I NaeI NarI NgoMIV NspI
PasI PciI PflMI PfoI PpuMI PspOMI PstI PvuII
SacI SanDI SapI SbfI SexAI SfcI SfiI SfoI
SgrAI SmaI SmlI SphI SrfI StyI TaqII TatI
TstI Tth111I XcmI XmaI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AgeI AhdI ApoI AscI
AseI AsiSI AvrII BaeI BclI BfrBI BglII BlpI
BmgBI BmtI BsaI BsaBI BsaWI BsiWI BsmI BspEI
BspHI BsrGI BssHII BstBI BstZ17I Bsu36I ClaI DraI
EagI EcoRI EcoRV FseI FspI FspAI HindIII HpaI
KpnI MfeI MluI NcoI NdeI NheI NotI NruI
NsiI PacI PmeI PmlI PpiI PshAI PsiI PspXI
PsrI PvuI RsrII SacII SalI ScaI SgfI SnaBI
SpeI SspI StuI SwaI XbaI XhoI XmnI ZraI