Restriction map for
pF1KA1745
(Linear) (Six-base) MAPSORT of: KIAA1745.seq Check: 9664 from: 1 to: 2511
KIAA1745.orf
With 182 enzymes: SgfI *
October 24, 2006 15:09 ..
Acc65I G'GTAC_C
Cuts at: 0 2357 2511
Size: 2357 154
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 182 444 774 1092 2263 2511
Size: 182 262 330 318 1171 248
Fragments arranged by size:
1171 330 318 262 248 182
AfeI AGC'GCT
Cuts at: 0 895 2511
Size: 895 1616
AleI CACnn'nnGTG
Cuts at: 0 832 1146 2511
Size: 832 314 1365
Fragments arranged by size:
1365 832 314
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1330 2511
Size: 1330 1181
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 799 831 2511
Size: 799 32 1680
Fragments arranged by size:
1680 799 32
AlwNI CAG_nnn'CTG
Cuts at: 0 321 924 1500 1592 1995 2009 2367 2511
Size: 321 603 576 92 403 14 358 144
Fragments arranged by size:
603 576 403 358 321 144 92 14
ApaI G_GGCC'C
Cuts at: 0 1441 1881 2330 2511
Size: 1441 440 449 181
Fragments arranged by size:
1441 449 440 181
ApaLI G'TGCA_C
Cuts at: 0 1447 2096 2163 2266 2511
Size: 1447 649 67 103 245
Fragments arranged by size:
1447 649 245 103 67
ApoI r'AATT_y
Cuts at: 0 567 808 2511
Size: 567 241 1703
Fragments arranged by size:
1703 567 241
AvaI C'yCGr_G
Cuts at: 0 257 1211 1442 1545 1613 2382 2390 2471
Size: 257 954 231 103 68 769 8 81
Cuts at: 2471 2511
Size: 40
Fragments arranged by size:
954 769 257 231 103 81 68 40
8
AvrII C'CTAG_G
Cuts at: 0 2322 2511
Size: 2322 189
BamHI G'GATC_C
Cuts at: 0 1694 2511
Size: 1694 817
BanI G'GyrC_C
Cuts at: 0 1170 1560 1939 2216 2357 2511
Size: 1170 390 379 277 141 154
Fragments arranged by size:
1170 390 379 277 154 141
BanII G_rGCy'C
Cuts at: 0 160 605 691 988 1441 1881 2330 2511
Size: 160 445 86 297 453 440 449 181
Fragments arranged by size:
453 449 445 440 297 181 160 86
BbsI GAAGACnn'nnnn_
Cuts at: 0 966 1020 1071 1098 1733 2229 2388 2511
Size: 966 54 51 27 635 496 159 123
Fragments arranged by size:
966 635 496 159 123 54 51 27
BbvCI CC'TCA_GC
Cuts at: 0 289 726 1673 2511
Size: 289 437 947 838
Fragments arranged by size:
947 838 437 289
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2325 2359 2511
Size: 2325 34 152
Fragments arranged by size:
2325 152 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 2460 2511
Size: 2460 51
BglI GCCn_nnn'nGGC
Cuts at: 0 63 2511
Size: 63 2448
BglII A'GATC_T
Cuts at: 0 776 1469 2511
Size: 776 693 1042
Fragments arranged by size:
1042 776 693
BlpI GC'TnA_GC
Cuts at: 0 130 223 421 982 2511
Size: 130 93 198 561 1529
Fragments arranged by size:
1529 561 198 130 93
Bme1580I G_kGCm'C
Cuts at: 0 1175 1212 1402 1441 1451 1565 1881 1942
Size: 1175 37 190 39 10 114 316 61
Cuts at: 1942 2100 2167 2270 2330 2464 2511
Size: 158 67 103 60 134 47
Fragments arranged by size:
1175 316 190 158 134 114 103 67
61 60 47 39 37 10
BmgBI CAC'GTC
Cuts at: 0 1656 2511
Size: 1656 855
BmrI ACTGGGnnnn_n'
Cuts at: 0 1032 1928 2188 2449 2511
Size: 1032 896 260 261 62
Fragments arranged by size:
1032 896 261 260 62
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 283 2511
Size: 283 2228
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1208 1626 2511
Size: 1208 418 885
Fragments arranged by size:
1208 885 418
Bpu10I CC'TnA_GC
Cuts at: 0 289 726 1673 2511
Size: 289 437 947 838
Fragments arranged by size:
947 838 437 289
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 895 1252 2511
Size: 895 357 1259
Fragments arranged by size:
1259 895 357
BsaI GGTCTCn'nnnn_
Cuts at: 0 2296 2511
Size: 2296 215
BsaAI yAC'GTr
Cuts at: 0 249 1908 2511
Size: 249 1659 603
Fragments arranged by size:
1659 603 249
BsaWI w'CCGG_w
Cuts at: 0 2219 2511
Size: 2219 292
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 866 896 1948 1978 2511
Size: 866 30 1052 30 533
Fragments arranged by size:
1052 866 533 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 39 1474 1597 1828 1993 2511
Size: 39 1435 123 231 165 518
Fragments arranged by size:
1435 518 231 165 123 39
BseYI C'CCAG_C
Cuts at: 0 711 917 988 1950 2120 2511
Size: 711 206 71 962 170 391
Fragments arranged by size:
962 711 391 206 170 71
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 353 378 1117 1354 1513 2147 2511
Size: 353 25 739 237 159 634 364
Fragments arranged by size:
739 634 364 353 237 159 25
BsiEI CG_ry'CG
Cuts at: 0 1413 2511
Size: 1413 1098
BsiHKAI G_wGCw'C
Cuts at: 0 258 605 691 931 1451 2100 2167 2270
Size: 258 347 86 240 520 649 67 103
Cuts at: 2270 2511
Size: 241
Fragments arranged by size:
649 520 347 258 241 240 103 86
67
Bsp1286I G_dGCh'C
Cuts at: 0 160 258 605 691 931 988 1175 1212
Size: 160 98 347 86 240 57 187 37
Cuts at: 1212 1402 1441 1451 1565 1881 1942 2100 2167
Size: 190 39 10 114 316 61 158 67
Cuts at: 2167 2270 2330 2464 2511
Size: 103 60 134 47
Fragments arranged by size:
347 316 240 190 187 160 158 134
114 103 98 86 67 61 60 57
47 39 37 10
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1284 1934 2287 2511
Size: 1284 650 353 224
Fragments arranged by size:
1284 650 353 224
BsrBI CCG'CTC
Cuts at: 0 168 420 879 1637 1836 2343 2511
Size: 168 252 459 758 199 507 168
Fragments arranged by size:
758 507 459 252 199 168 168
BsrDI GCAATG_nn'
Cuts at: 0 842 2511
Size: 842 1669
BsrFI r'CCGG_y
Cuts at: 0 1412 2213 2511
Size: 1412 801 298
Fragments arranged by size:
1412 801 298
BsrGI T'GTAC_A
Cuts at: 0 1350 1583 2511
Size: 1350 233 928
Fragments arranged by size:
1350 928 233
BssHII G'CGCG_C
Cuts at: 0 50 2511
Size: 50 2461
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1500 2511
Size: 1500 1011
BstBI TT'CG_AA
Cuts at: 0 185 2511
Size: 185 2326
BstXI CCAn_nnnn'nTGG
Cuts at: 0 585 2511
Size: 585 1926
BstYI r'GATC_y
Cuts at: 0 407 776 1469 1694 2492 2511
Size: 407 369 693 225 798 19
Fragments arranged by size:
798 693 407 369 225 19
Bsu36I CC'TnA_GG
Cuts at: 0 22 2511
Size: 22 2489
BtgI C'CryG_G
Cuts at: 0 43 1565 1690 2511
Size: 43 1522 125 821
Fragments arranged by size:
1522 821 125 43
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 28 779 878 2069 2511
Size: 28 751 99 1191 442
Fragments arranged by size:
1191 751 442 99 28
BtsI GCAGTG_nn'
Cuts at: 0 361 578 1153 1433 2067 2511
Size: 361 217 575 280 634 444
Fragments arranged by size:
634 575 444 361 280 217
DraIII CAC_nnn'GTG
Cuts at: 0 1170 2511
Size: 1170 1341
DrdI GACnn_nn'nnGTC
Cuts at: 0 1294 2511
Size: 1294 1217
EaeI y'GGCC_r
Cuts at: 0 64 169 644 801 1410 1568 1679 2180
Size: 64 105 475 157 609 158 111 501
Cuts at: 2180 2511
Size: 331
Fragments arranged by size:
609 501 475 331 158 157 111 105
64
EagI C'GGCC_G
Cuts at: 0 1410 2511
Size: 1410 1101
EarI CTCTTCn'nnn_
Cuts at: 0 158 1396 2407 2511
Size: 158 1238 1011 104
Fragments arranged by size:
1238 1011 158 104
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2467 2511
Size: 2467 44
EcoICRI GAG'CTC
Cuts at: 0 603 689 2511
Size: 603 86 1822
Fragments arranged by size:
1822 603 86
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 182 444 774 1092 1208 1626 2263 2511
Size: 182 262 330 318 116 418 637 248
Fragments arranged by size:
637 418 330 318 262 248 182 116
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1120 2245 2511
Size: 1120 1125 266
Fragments arranged by size:
1125 1120 266
EcoO109I rG'GnC_Cy
Cuts at: 0 239 264 375 1438 1617 1725 1877 1878
Size: 239 25 111 1063 179 108 152 1
Cuts at: 1878 1923 2135 2326 2327 2387 2511
Size: 45 212 191 1 60 124
Fragments arranged by size:
1063 239 212 191 179 152 124 111
108 60 45 25 1 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 798 1293 2511
Size: 798 495 1218
Fragments arranged by size:
1218 798 495
FspI TGC'GCA
Cuts at: 0 7 2511
Size: 7 2504
HaeII r_GCGC'y
Cuts at: 0 130 897 1721 2511
Size: 130 767 824 790
Fragments arranged by size:
824 790 767 130
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1181 1213 2511
Size: 1181 32 1298
Fragments arranged by size:
1298 1181 32
KpnI G_GTAC'C
Cuts at: 0 2361 2511
Size: 2361 150
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 142 227 1868 2511
Size: 142 85 1641 643
Fragments arranged by size:
1641 643 142 85
MscI TGG'CCA
Cuts at: 0 803 1570 1681 2511
Size: 803 767 111 830
Fragments arranged by size:
830 803 767 111
MslI CAynn'nnrTG
Cuts at: 0 13 484 832 1146 2511
Size: 13 471 348 314 1365
Fragments arranged by size:
1365 471 348 314 13
MspA1I CmG'CkG
Cuts at: 0 72 425 921 1112 1646 1677 1950 1995
Size: 72 353 496 191 534 31 273 45
Cuts at: 1995 2104 2120 2511
Size: 109 16 391
Fragments arranged by size:
534 496 391 353 273 191 109 72
45 31 16
NaeI GCC'GGC
Cuts at: 0 1414 2511
Size: 1414 1097
NcoI C'CATG_G
Cuts at: 0 43 1565 2511
Size: 43 1522 946
Fragments arranged by size:
1522 946 43
NgoMIV G'CCGG_C
Cuts at: 0 1412 2511
Size: 1412 1099
NspI r_CATG'y
Cuts at: 0 1311 2511
Size: 1311 1200
PflMI CCAn_nnn'nTGG
Cuts at: 0 1344 1691 1950 1995 2107 2511
Size: 1344 347 259 45 112 404
Fragments arranged by size:
1344 404 347 259 112 45
PfoI T'CCnGG_A
Cuts at: 0 530 1505 1697 2511
Size: 530 975 192 814
Fragments arranged by size:
975 814 530 192
PmlI CAC'GTG
Cuts at: 0 1908 2511
Size: 1908 603
PpuMI rG'GwC_Cy
Cuts at: 0 239 375 1617 1725 1923 2135 2387 2511
Size: 239 136 1242 108 198 212 252 124
Fragments arranged by size:
1242 252 239 212 198 136 124 108
PspOMI G'GGCC_C
Cuts at: 0 1437 1877 2326 2511
Size: 1437 440 449 185
Fragments arranged by size:
1437 449 440 185
PstI C_TGCA'G
Cuts at: 0 107 968 1241 1322 1469 1579 2511
Size: 107 861 273 81 147 110 932
Fragments arranged by size:
932 861 273 147 110 107 81
PvuII CAG'CTG
Cuts at: 0 921 1646 1677 1950 1995 2104 2120 2511
Size: 921 725 31 273 45 109 16 391
Fragments arranged by size:
921 725 391 273 109 45 31 16
SacI G_AGCT'C
Cuts at: 0 605 691 2511
Size: 605 86 1820
Fragments arranged by size:
1820 605 86
SanDI GG'GwC_CC
Cuts at: 0 1617 2387 2511
Size: 1617 770 124
Fragments arranged by size:
1617 770 124
SapI GCTCTTCn'nnn_
Cuts at: 0 158 2511
Size: 158 2353
SfcI C'TryA_G
Cuts at: 0 103 886 964 1055 1237 1318 1465 1575
Size: 103 783 78 91 182 81 147 110
Cuts at: 1575 2511
Size: 936
Fragments arranged by size:
936 783 182 147 110 103 91 81
78
SmaI CCC'GGG
Cuts at: 0 1213 1444 1615 2384 2473 2511
Size: 1213 231 171 769 89 38
Fragments arranged by size:
1213 769 231 171 89 38
SmlI C'TyrA_G
Cuts at: 0 257 910 1267 2511
Size: 257 653 357 1244
Fragments arranged by size:
1244 653 357 257
SphI G_CATG'C
Cuts at: 0 1311 2511
Size: 1311 1200
StyI C'CwwG_G
Cuts at: 0 43 630 1565 1721 2322 2481 2511
Size: 43 587 935 156 601 159 30
Fragments arranged by size:
935 601 587 159 156 43 30
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 241 697 1528 1957 2511
Size: 241 456 831 429 554
Fragments arranged by size:
831 554 456 429 241
TatI w'GTAC_w
Cuts at: 0 1350 1583 2511
Size: 1350 233 928
Fragments arranged by size:
1350 928 233
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 866 898 1475 1507 1948 1980 2411 2443
Size: 866 32 577 32 441 32 431 32
Cuts at: 2443 2511
Size: 68
Fragments arranged by size:
866 577 441 431 68 32 32 32
32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 585 2511
Size: 585 1926
XhoI C'TCGA_G
Cuts at: 0 257 2511
Size: 257 2254
XmaI C'CCGG_G
Cuts at: 0 1211 1442 1613 2382 2471 2511
Size: 1211 231 171 769 89 40
Fragments arranged by size:
1211 769 231 171 89 40
XmnI GAAnn'nnTTC
Cuts at: 0 2149 2236 2511
Size: 2149 87 275
Fragments arranged by size:
2149 275 87
Enzymes that do cut:
Acc65I AcuI AfeI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AvaI AvrII BamHI BanI BanII BbsI
BbvCI BcgI BciVI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BstAPI BstBI BstXI
BstYI Bsu36I BtgI BtgZI BtsI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI
FspI HaeII Hin4I KpnI MmeI MscI MslI MspA1I
NaeI NcoI NgoMIV NspI PflMI PfoI PmlI PpuMI
PspOMI PstI PvuII SacI SanDI SapI SfcI SmaI
SmlI SphI StyI TaqII TatI TstI XcmI XhoI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AflII AflIII AgeI AhdI
AscI AseI AsiSI BaeI BbeI BclI BfrBI BmtI
BsaBI BsaHI BsiWI BsmI BsmBI BspEI BspHI BssSI
BstEII BstZ17I ClaI CspCI DraI EcoRI EcoRV FseI
FspAI HincII HindIII HpaI KasI MfeI MluI NarI
NdeI NheI NotI NruI NsiI PacI PasI PciI
PmeI PpiI PshAI PsiI PspXI PsrI PvuI RsrII
SacII SalI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SnaBI SpeI SrfI SspI StuI SwaI Tth111I
XbaI ZraI