Restriction map for
pF1KA1746
(Linear) (Six-base) MAPSORT of: KIAA1746.seq Check: 2048 from: 1 to: 3282
KIAA1746.orf
With 182 enzymes: SgfI *
March 3, 2008 10:55 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 732 979 3149 3282
Size: 732 247 2170 133
Fragments arranged by size:
2170 732 247 133
AatII G_ACGT'C
Cuts at: 0 2723 3282
Size: 2723 559
Acc65I G'GTAC_C
Cuts at: 0 1314 3282
Size: 1314 1968
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 205 764 2173 3166 3282
Size: 205 559 1409 993 116
Fragments arranged by size:
1409 993 559 205 116
AflIII A'CryG_T
Cuts at: 0 3060 3282
Size: 3060 222
AhdI GACnn_n'nnGTC
Cuts at: 0 44 1743 3282
Size: 44 1699 1539
Fragments arranged by size:
1699 1539 44
AleI CACnn'nnGTG
Cuts at: 0 862 1990 3282
Size: 862 1128 1292
Fragments arranged by size:
1292 1128 862
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 696 2450 3282
Size: 696 1754 832
Fragments arranged by size:
1754 832 696
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 812 844 2105 2137 2858 2890 3282
Size: 812 32 1261 32 721 32 392
Fragments arranged by size:
1261 812 721 392 32 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1134 2076 2961 3255 3282
Size: 1134 942 885 294 27
Fragments arranged by size:
1134 942 885 294 27
ApaI G_GGCC'C
Cuts at: 0 891 3282
Size: 891 2391
ApoI r'AATT_y
Cuts at: 0 520 2549 3282
Size: 520 2029 733
Fragments arranged by size:
2029 733 520
AvaI C'yCGr_G
Cuts at: 0 87 96 1732 3282
Size: 87 9 1636 1550
Fragments arranged by size:
1636 1550 87 9
AvrII C'CTAG_G
Cuts at: 0 367 3282
Size: 367 2915
BamHI G'GATC_C
Cuts at: 0 189 3282
Size: 189 3093
BanI G'GyrC_C
Cuts at: 0 526 1314 3282
Size: 526 788 1968
Fragments arranged by size:
1968 788 526
BanII G_rGCy'C
Cuts at: 0 33 88 103 400 891 1850 2468 2980
Size: 33 55 15 297 491 959 618 512
Cuts at: 2980 3170 3282
Size: 190 112
Fragments arranged by size:
959 618 512 491 297 190 112 55
33 15
BbsI GAAGACnn'nnnn_
Cuts at: 0 433 585 874 2675 3282
Size: 433 152 289 1801 607
Fragments arranged by size:
1801 607 433 289 152
BbvCI CC'TCA_GC
Cuts at: 0 1019 3282
Size: 1019 2263
BciVI GTATCCnnnnn_n'
Cuts at: 0 1713 2193 3282
Size: 1713 480 1089
Fragments arranged by size:
1713 1089 480
BfrBI ATG'CAT
Cuts at: 0 1358 3282
Size: 1358 1924
BglI GCCn_nnn'nGGC
Cuts at: 0 13 92 2047 2269 3199 3282
Size: 13 79 1955 222 930 83
Fragments arranged by size:
1955 930 222 83 79 13
BglII A'GATC_T
Cuts at: 0 2310 3282
Size: 2310 972
BlpI GC'TnA_GC
Cuts at: 0 82 617 2077 3112 3282
Size: 82 535 1460 1035 170
Fragments arranged by size:
1460 1035 535 170 82
Bme1580I G_kGCm'C
Cuts at: 0 891 1592 3282
Size: 891 701 1690
Fragments arranged by size:
1690 891 701
BmgBI CAC'GTC
Cuts at: 0 543 3177 3282
Size: 543 2634 105
Fragments arranged by size:
2634 543 105
BmrI ACTGGGnnnn_n'
Cuts at: 0 697 843 3189 3282
Size: 697 146 2346 93
Fragments arranged by size:
2346 697 146 93
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1496 1869 3282
Size: 1496 373 1413
Fragments arranged by size:
1496 1413 373
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 817 1234 1839 3184 3277 3282
Size: 817 417 605 1345 93 5
Fragments arranged by size:
1345 817 605 417 93 5
Bpu10I CC'TnA_GC
Cuts at: 0 32 394 807 1019 3282
Size: 32 362 413 212 2263
Fragments arranged by size:
2263 413 362 212 32
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 87 795 1613 2435 2623 2638 3109 3282
Size: 87 708 818 822 188 15 471 173
Fragments arranged by size:
822 818 708 471 188 173 87 15
BsaI GGTCTCn'nnnn_
Cuts at: 0 936 2785 2856 3282
Size: 936 1849 71 426
Fragments arranged by size:
1849 936 426 71
BsaAI yAC'GTr
Cuts at: 0 1383 3153 3282
Size: 1383 1770 129
Fragments arranged by size:
1770 1383 129
BsaBI GATnn'nnATC
Cuts at: 0 1648 3282
Size: 1648 1634
BsaHI Gr'CG_yC
Cuts at: 0 906 2720 3282
Size: 906 1814 562
Fragments arranged by size:
1814 906 562
BsaWI w'CCGG_w
Cuts at: 0 375 2202 3282
Size: 375 1827 1080
Fragments arranged by size:
1827 1080 375
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 163 193 2105 2135 2834 2864 3282
Size: 163 30 1912 30 699 30 418
Fragments arranged by size:
1912 699 418 163 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 763 1256 2129 3282
Size: 763 493 873 1153
Fragments arranged by size:
1153 873 763 493
BseYI C'CCAG_C
Cuts at: 0 891 1082 2467 2973 3282
Size: 891 191 1385 506 309
Fragments arranged by size:
1385 891 506 309 191
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 840 3282
Size: 840 2442
BsiHKAI G_wGCw'C
Cuts at: 0 2059 2916 3282
Size: 2059 857 366
Fragments arranged by size:
2059 857 366
BsmI GAATG_Cn'
Cuts at: 0 1765 3282
Size: 1765 1517
Bsp1286I G_dGCh'C
Cuts at: 0 33 88 103 400 891 1592 1850 2059
Size: 33 55 15 297 491 701 258 209
Cuts at: 2059 2468 2916 2980 3170 3282
Size: 409 448 64 190 112
Fragments arranged by size:
701 491 448 409 297 258 209 190
112 64 55 33 15
BspEI T'CCGG_A
Cuts at: 0 375 3282
Size: 375 2907
BspHI T'CATG_A
Cuts at: 0 2349 3224 3282
Size: 2349 875 58
Fragments arranged by size:
2349 875 58
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 664 732 979 3149 3282
Size: 664 68 247 2170 133
Fragments arranged by size:
2170 664 247 133 68
BsrBI CCG'CTC
Cuts at: 0 1914 3282
Size: 1914 1368
BsrDI GCAATG_nn'
Cuts at: 0 2014 3282
Size: 2014 1268
BsrGI T'GTAC_A
Cuts at: 0 584 3282
Size: 584 2698
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2163 3282
Size: 2163 1119
BstXI CCAn_nnnn'nTGG
Cuts at: 0 658 2094 2931 3063 3282
Size: 658 1436 837 132 219
Fragments arranged by size:
1436 837 658 219 132
BstYI r'GATC_y
Cuts at: 0 189 2310 2901 3129 3282
Size: 189 2121 591 228 153
Fragments arranged by size:
2121 591 228 189 153
Bsu36I CC'TnA_GG
Cuts at: 0 2887 3282
Size: 2887 395
BtgI C'CryG_G
Cuts at: 0 17 2060 2087 2250 2981 3282
Size: 17 2043 27 163 731 301
Fragments arranged by size:
2043 731 301 163 27 17
BtsI GCAGTG_nn'
Cuts at: 0 1749 3282
Size: 1749 1533
ClaI AT'CG_AT
Cuts at: 0 1275 3282
Size: 1275 2007
DraI TTT'AAA
Cuts at: 0 149 3282
Size: 149 3133
DraIII CAC_nnn'GTG
Cuts at: 0 3156 3282
Size: 3156 126
EaeI y'GGCC_r
Cuts at: 0 247 2933 3017 3179 3282
Size: 247 2686 84 162 103
Fragments arranged by size:
2686 247 162 103 84
EarI CTCTTCn'nnn_
Cuts at: 0 725 740 1274 1898 2324 3203 3282
Size: 725 15 534 624 426 879 79
Fragments arranged by size:
879 725 624 534 426 79 15
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 319 3282
Size: 319 2963
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 205 764 817 1234 1839 2173 3166 3184
Size: 205 559 53 417 605 334 993 18
Cuts at: 3184 3277 3282
Size: 93 5
Fragments arranged by size:
993 605 559 417 334 205 93 53
18 5
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2886 3282
Size: 2886 396
EcoO109I rG'GnC_Cy
Cuts at: 0 600 770 888 3282
Size: 600 170 118 2394
Fragments arranged by size:
2394 600 170 118
EcoRV GAT'ATC
Cuts at: 0 666 3282
Size: 666 2616
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1363 1884 2542 2650 2664 3282
Size: 1363 521 658 108 14 618
Fragments arranged by size:
1363 658 618 521 108 14
HaeII r_GCGC'y
Cuts at: 0 3119 3282
Size: 3119 163
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 30 62 867 899 908 940 1729 1761
Size: 30 32 805 32 9 32 789 32
Cuts at: 1761 1945 1977 2167 2199 2834 2836 2866 2868
Size: 184 32 190 32 635 2 30 2
Cuts at: 2868 3282
Size: 414
Fragments arranged by size:
805 789 635 414 190 184 32 32
32 32 32 32 30 30 9 2
2
HincII GTy'rAC
Cuts at: 0 1805 3282
Size: 1805 1477
HindIII A'AGCT_T
Cuts at: 0 2632 3184 3282
Size: 2632 552 98
Fragments arranged by size:
2632 552 98
KpnI G_GTAC'C
Cuts at: 0 1318 3282
Size: 1318 1964
MfeI C'AATT_G
Cuts at: 0 1547 3282
Size: 1547 1735
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 474 1556 2153 2210 2578 3282
Size: 474 1082 597 57 368 704
Fragments arranged by size:
1082 704 597 474 368 57
MscI TGG'CCA
Cuts at: 0 249 2935 3019 3181 3282
Size: 249 2686 84 162 101
Fragments arranged by size:
2686 249 162 101 84
MslI CAynn'nnrTG
Cuts at: 0 862 1990 2086 3061 3282
Size: 862 1128 96 975 221
Fragments arranged by size:
1128 975 862 221 96
MspA1I CmG'CkG
Cuts at: 0 26 593 921 1003 1935 3282
Size: 26 567 328 82 932 1347
Fragments arranged by size:
1347 932 567 328 82 26
NcoI C'CATG_G
Cuts at: 0 2087 2250 3282
Size: 2087 163 1032
Fragments arranged by size:
2087 1032 163
NsiI A_TGCA'T
Cuts at: 0 1360 3282
Size: 1360 1922
NspI r_CATG'y
Cuts at: 0 44 786 2085 3064 3282
Size: 44 742 1299 979 218
Fragments arranged by size:
1299 979 742 218 44
PciI A'CATG_T
Cuts at: 0 3060 3282
Size: 3060 222
PflMI CCAn_nnn'nTGG
Cuts at: 0 1134 2961 3282
Size: 1134 1827 321
Fragments arranged by size:
1827 1134 321
PfoI T'CCnGG_A
Cuts at: 0 815 3282
Size: 815 2467
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2105 2137 3282
Size: 2105 32 1145
Fragments arranged by size:
2105 1145 32
PsiI TTA'TAA
Cuts at: 0 1204 3282
Size: 1204 2078
PspOMI G'GGCC_C
Cuts at: 0 887 3282
Size: 887 2395
PstI C_TGCA'G
Cuts at: 0 729 1934 2429 3282
Size: 729 1205 495 853
Fragments arranged by size:
1205 853 729 495
PvuII CAG'CTG
Cuts at: 0 593 921 1003 1935 3282
Size: 593 328 82 932 1347
Fragments arranged by size:
1347 932 593 328 82
SapI GCTCTTCn'nnn_
Cuts at: 0 2324 3203 3282
Size: 2324 879 79
Fragments arranged by size:
2324 879 79
SfcI C'TryA_G
Cuts at: 0 112 725 1110 1930 2425 3282
Size: 112 613 385 820 495 857
Fragments arranged by size:
857 820 613 495 385 112
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 13 3282
Size: 13 3269
SmaI CCC'GGG
Cuts at: 0 89 3282
Size: 89 3193
SmlI C'TyrA_G
Cuts at: 0 102 774 1628 2450 2617 2638 3124 3282
Size: 102 672 854 822 167 21 486 158
Fragments arranged by size:
854 822 672 486 167 158 102 21
StuI AGG'CCT
Cuts at: 0 2423 3282
Size: 2423 859
StyI C'CwwG_G
Cuts at: 0 367 461 2087 2250 2940 3282
Size: 367 94 1626 163 690 342
Fragments arranged by size:
1626 690 367 342 163 94
TatI w'GTAC_w
Cuts at: 0 584 2293 3282
Size: 584 1709 989
Fragments arranged by size:
1709 989 584
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 181 213 1032 1064 3282
Size: 181 32 819 32 2218
Fragments arranged by size:
2218 819 181 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 2848 3282
Size: 2848 434
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2094 3282
Size: 2094 1188
XmaI C'CCGG_G
Cuts at: 0 87 3282
Size: 87 3195
XmnI GAAnn'nnTTC
Cuts at: 0 734 3232 3282
Size: 734 2498 50
Fragments arranged by size:
2498 734 50
ZraI GAC'GTC
Cuts at: 0 2721 3282
Size: 2721 561
Enzymes that do cut:
AarI AatII Acc65I AcuI AflIII AhdI AleI AlfI
AloI AlwNI ApaI ApoI AvaI AvrII BamHI BanI
BanII BbsI BbvCI BciVI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrGI BstAPI BstXI BstYI Bsu36I BtgI BtsI ClaI
DraI DraIII EaeI EarI EciI Eco57MI EcoNI EcoO109I
EcoRV FalI HaeII Hin4I HincII HindIII KpnI MfeI
MmeI MscI MslI MspA1I NcoI NsiI NspI PciI
PflMI PfoI PpiI PsiI PspOMI PstI PvuII SapI
SfcI SfiI SmaI SmlI StuI StyI TatI TstI
Tth111I XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AccI AclI AfeI AflII AgeI ApaLI AscI AseI
AsiSI BaeI BbeI BcgI BclI BmtI BsiEI BsiWI
BsmBI BsrFI BssHII BssSI BstBI BstEII BstZ17I BtgZI
CspCI DrdI EagI EcoICRI EcoRI FseI FspI FspAI
HpaI KasI MluI NaeI NarI NdeI NgoMIV NheI
NotI NruI PacI PasI PmeI PmlI PpuMI PshAI
PspXI PsrI PvuI RsrII SacI SacII SalI SanDI
SbfI ScaI SexAI SfoI SgfI SgrAI SnaBI SpeI
SphI SrfI SspI SwaI TaqII XbaI XhoI