Restriction map for
pF1KA1747
(Linear) (Six-base) MAPSORT of: KIAA1747.seq Check: 9308 from: 1 to: 2349
KIAA1747.orf
With 182 enzymes: SgfI *
October 24, 2006 15:09 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1339 2349
Size: 1339 1010
Acc65I G'GTAC_C
Cuts at: 0 230 2349
Size: 230 2119
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 306 763 1087 1681 2349
Size: 306 457 324 594 668
Fragments arranged by size:
668 594 457 324 306
AfeI AGC'GCT
Cuts at: 0 811 1195 2349
Size: 811 384 1154
Fragments arranged by size:
1154 811 384
AflII C'TTAA_G
Cuts at: 0 2245 2349
Size: 2245 104
AgeI A'CCGG_T
Cuts at: 0 685 1049 2349
Size: 685 364 1300
Fragments arranged by size:
1300 685 364
AleI CACnn'nnGTG
Cuts at: 0 562 2349
Size: 562 1787
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1621 2349
Size: 1621 728
AlwNI CAG_nnn'CTG
Cuts at: 0 498 786 1026 1122 1467 1533 2349
Size: 498 288 240 96 345 66 816
Fragments arranged by size:
816 498 345 288 240 96 66
ApaI G_GGCC'C
Cuts at: 0 190 1363 1391 2349
Size: 190 1173 28 958
Fragments arranged by size:
1173 958 190 28
ApaLI G'TGCA_C
Cuts at: 0 1690 2349
Size: 1690 659
ApoI r'AATT_y
Cuts at: 0 2019 2349
Size: 2019 330
AvaI C'yCGr_G
Cuts at: 0 127 1443 2349
Size: 127 1316 906
Fragments arranged by size:
1316 906 127
BamHI G'GATC_C
Cuts at: 0 1163 2273 2349
Size: 1163 1110 76
Fragments arranged by size:
1163 1110 76
BanI G'GyrC_C
Cuts at: 0 230 526 2349
Size: 230 296 1823
Fragments arranged by size:
1823 296 230
BanII G_rGCy'C
Cuts at: 0 190 863 1363 1391 1994 2168 2349
Size: 190 673 500 28 603 174 181
Fragments arranged by size:
673 603 500 190 181 174 28
BbsI GAAGACnn'nnnn_
Cuts at: 0 1912 2349
Size: 1912 437
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 118 152 2349
Size: 118 34 2197
Fragments arranged by size:
2197 118 34
BglI GCCn_nnn'nGGC
Cuts at: 0 67 83 112 658 2349
Size: 67 16 29 546 1691
Fragments arranged by size:
1691 546 67 29 16
BglII A'GATC_T
Cuts at: 0 1718 2349
Size: 1718 631
Bme1580I G_kGCm'C
Cuts at: 0 190 1363 1391 1401 1694 2349
Size: 190 1173 28 10 293 655
Fragments arranged by size:
1173 655 293 190 28 10
BmrI ACTGGGnnnn_n'
Cuts at: 0 82 508 1071 1845 1860 2349
Size: 82 426 563 774 15 489
Fragments arranged by size:
774 563 489 426 82 15
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1599 2349
Size: 1599 750
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 592 1394 1534 1555 1656 1765 2017 2089
Size: 592 802 140 21 101 109 252 72
Cuts at: 2089 2349
Size: 260
Fragments arranged by size:
802 592 260 252 140 109 101 72
21
Bpu10I CC'TnA_GC
Cuts at: 0 203 2167 2349
Size: 203 1964 182
Fragments arranged by size:
1964 203 182
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2111 2349
Size: 2111 238
BsaI GGTCTCn'nnnn_
Cuts at: 0 375 568 670 2323 2349
Size: 375 193 102 1653 26
Fragments arranged by size:
1653 375 193 102 26
BsaAI yAC'GTr
Cuts at: 0 1695 2349
Size: 1695 654
BsaWI w'CCGG_w
Cuts at: 0 356 685 1049 2349
Size: 356 329 364 1300
Fragments arranged by size:
1300 364 356 329
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1528 1558 2206 2236 2349
Size: 1528 30 648 30 113
Fragments arranged by size:
1528 648 113 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 473 1261 2349
Size: 473 788 1088
Fragments arranged by size:
1088 788 473
BseYI C'CCAG_C
Cuts at: 0 86 1122 1611 2349
Size: 86 1036 489 738
Fragments arranged by size:
1036 738 489 86
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 565 619 915 1311 2129 2349
Size: 565 54 296 396 818 220
Fragments arranged by size:
818 565 396 296 220 54
BsiEI CG_ry'CG
Cuts at: 0 40 2349
Size: 40 2309
BsiHKAI G_wGCw'C
Cuts at: 0 622 863 1410 1694 2168 2349
Size: 622 241 547 284 474 181
Fragments arranged by size:
622 547 474 284 241 181
BsmI GAATG_Cn'
Cuts at: 0 919 2349
Size: 919 1430
Bsp1286I G_dGCh'C
Cuts at: 0 190 622 863 1363 1391 1401 1410 1694
Size: 190 432 241 500 28 10 9 284
Cuts at: 1694 1994 2168 2349
Size: 300 174 181
Fragments arranged by size:
500 432 300 284 241 190 181 174
28 10 9
BspEI T'CCGG_A
Cuts at: 0 356 2349
Size: 356 1993
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1339 1556 2040 2349
Size: 1339 217 484 309
Fragments arranged by size:
1339 484 309 217
BsrBI CCG'CTC
Cuts at: 0 228 2349
Size: 228 2121
BsrDI GCAATG_nn'
Cuts at: 0 331 1605 2349
Size: 331 1274 744
Fragments arranged by size:
1274 744 331
BsrFI r'CCGG_y
Cuts at: 0 39 685 992 1049 1172 1472 2332 2349
Size: 39 646 307 57 123 300 860 17
Fragments arranged by size:
860 646 307 300 123 57 39 17
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1196 2349
Size: 1196 1153
BstEII G'GTnAC_C
Cuts at: 0 669 2349
Size: 669 1680
BstXI CCAn_nnnn'nTGG
Cuts at: 0 483 2349
Size: 483 1866
BstYI r'GATC_y
Cuts at: 0 965 1163 1718 2273 2349
Size: 965 198 555 555 76
Fragments arranged by size:
965 555 555 198 76
Bsu36I CC'TnA_GG
Cuts at: 0 2287 2349
Size: 2287 62
BtgI C'CryG_G
Cuts at: 0 52 107 653 944 1088 2349
Size: 52 55 546 291 144 1261
Fragments arranged by size:
1261 546 291 144 55 52
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1186 2349
Size: 1186 1163
BtsI GCAGTG_nn'
Cuts at: 0 16 552 2349
Size: 16 536 1797
Fragments arranged by size:
1797 536 16
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2300 2335 2349
Size: 2300 35 14
Fragments arranged by size:
2300 35 14
DraIII CAC_nnn'GTG
Cuts at: 0 1429 2349
Size: 1429 920
EaeI y'GGCC_r
Cuts at: 0 37 150 590 1474 1484 1757 2252 2349
Size: 37 113 440 884 10 273 495 97
Fragments arranged by size:
884 495 440 273 113 97 37 10
EagI C'GGCC_G
Cuts at: 0 37 2349
Size: 37 2312
EarI CTCTTCn'nnn_
Cuts at: 0 458 998 1007 1183 1657 2349
Size: 458 540 9 176 474 692
Fragments arranged by size:
692 540 474 458 176 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1207 2349
Size: 1207 1142
EcoICRI GAG'CTC
Cuts at: 0 861 2166 2349
Size: 861 1305 183
Fragments arranged by size:
1305 861 183
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 306 592 763 1087 1394 1534 1555 1656
Size: 306 286 171 324 307 140 21 101
Cuts at: 1656 1681 1765 2017 2089 2349
Size: 25 84 252 72 260
Fragments arranged by size:
324 307 306 286 260 252 171 140
101 84 72 25 21
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1225 2286 2349
Size: 1225 1061 63
Fragments arranged by size:
1225 1061 63
EcoO109I rG'GnC_Cy
Cuts at: 0 186 187 1023 1235 1359 1360 1387 1530
Size: 186 1 836 212 124 1 27 143
Cuts at: 1530 2349
Size: 819
Fragments arranged by size:
836 819 212 186 143 124 27 1
1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 336 1929 2227 2349
Size: 336 1593 298 122
Fragments arranged by size:
1593 336 298 122
HaeII r_GCGC'y
Cuts at: 0 813 1197 2349
Size: 813 384 1152
Fragments arranged by size:
1152 813 384
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 372 404 675 707 1951 1983 1992 2024
Size: 372 32 271 32 1244 32 9 32
Cuts at: 2024 2206 2238 2349
Size: 182 32 111
Fragments arranged by size:
1244 372 271 182 111 32 32 32
32 32 9
HincII GTy'rAC
Cuts at: 0 1770 2349
Size: 1770 579
KpnI G_GTAC'C
Cuts at: 0 234 2349
Size: 234 2115
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 262 2090 2232 2349
Size: 262 1828 142 117
Fragments arranged by size:
1828 262 142 117
MscI TGG'CCA
Cuts at: 0 152 1759 2254 2349
Size: 152 1607 495 95
Fragments arranged by size:
1607 495 152 95
MslI CAynn'nnrTG
Cuts at: 0 481 562 1810 2349
Size: 481 81 1248 539
Fragments arranged by size:
1248 539 481 81
MspA1I CmG'CkG
Cuts at: 0 562 588 1122 1325 1619 1861 2349
Size: 562 26 534 203 294 242 488
Fragments arranged by size:
562 534 488 294 242 203 26
NaeI GCC'GGC
Cuts at: 0 41 1174 1474 2349
Size: 41 1133 300 875
Fragments arranged by size:
1133 875 300 41
NcoI C'CATG_G
Cuts at: 0 52 944 1088 2349
Size: 52 892 144 1261
Fragments arranged by size:
1261 892 144 52
NgoMIV G'CCGG_C
Cuts at: 0 39 1172 1472 2349
Size: 39 1133 300 877
Fragments arranged by size:
1133 877 300 39
NspI r_CATG'y
Cuts at: 0 139 845 1608 1650 2349
Size: 139 706 763 42 699
Fragments arranged by size:
763 706 699 139 42
PasI CC'CwG_GG
Cuts at: 0 1461 2349
Size: 1461 888
PflMI CCAn_nnn'nTGG
Cuts at: 0 945 1122 2349
Size: 945 177 1227
Fragments arranged by size:
1227 945 177
PfoI T'CCnGG_A
Cuts at: 0 968 1307 1633 2090 2349
Size: 968 339 326 457 259
Fragments arranged by size:
968 457 339 326 259
PmlI CAC'GTG
Cuts at: 0 1695 2349
Size: 1695 654
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1786 1818 2349
Size: 1786 32 531
Fragments arranged by size:
1786 531 32
PpuMI rG'GwC_Cy
Cuts at: 0 1235 1530 2349
Size: 1235 295 819
Fragments arranged by size:
1235 819 295
PspOMI G'GGCC_C
Cuts at: 0 186 1359 1387 2349
Size: 186 1173 28 962
Fragments arranged by size:
1173 962 186 28
PstI C_TGCA'G
Cuts at: 0 703 825 965 1556 2349
Size: 703 122 140 591 793
Fragments arranged by size:
793 703 591 140 122
PvuII CAG'CTG
Cuts at: 0 562 588 1122 1325 1619 1861 2349
Size: 562 26 534 203 294 242 488
Fragments arranged by size:
562 534 488 294 242 203 26
SacI G_AGCT'C
Cuts at: 0 863 2168 2349
Size: 863 1305 181
Fragments arranged by size:
1305 863 181
SapI GCTCTTCn'nnn_
Cuts at: 0 1183 1657 2349
Size: 1183 474 692
Fragments arranged by size:
1183 692 474
SexAI A'CCwGG_T
Cuts at: 0 250 2349
Size: 250 2099
SfcI C'TryA_G
Cuts at: 0 699 821 961 1552 2061 2343 2349
Size: 699 122 140 591 509 282 6
Fragments arranged by size:
699 591 509 282 140 122 6
SmaI CCC'GGG
Cuts at: 0 1445 2349
Size: 1445 904
SmlI C'TyrA_G
Cuts at: 0 2126 2245 2349
Size: 2126 119 104
Fragments arranged by size:
2126 119 104
SphI G_CATG'C
Cuts at: 0 139 1650 2349
Size: 139 1511 699
Fragments arranged by size:
1511 699 139
StyI C'CwwG_G
Cuts at: 0 52 372 453 944 1088 1226 1376 1889
Size: 52 320 81 491 144 138 150 513
Cuts at: 1889 2349
Size: 460
Fragments arranged by size:
513 491 460 320 150 144 138 81
52
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 78 2349
Size: 78 2271
TatI w'GTAC_w
Cuts at: 0 762 1673 2349
Size: 762 911 676
Fragments arranged by size:
911 762 676
Tth111I GACn'n_nGTC
Cuts at: 0 724 2349
Size: 724 1625
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2262 2349
Size: 2262 87
XmaI C'CCGG_G
Cuts at: 0 1443 2349
Size: 1443 906
XmnI GAAnn'nnTTC
Cuts at: 0 316 1502 1909 2349
Size: 316 1186 407 440
Fragments arranged by size:
1186 440 407 316
Enzymes that do cut:
AarI Acc65I AcuI AfeI AflII AgeI AleI AlfI
AlwNI ApaI ApaLI ApoI AvaI BamHI BanI BanII
BbsI BcgI BglI BglII Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspMI BsrBI
BsrDI BsrFI BstAPI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI CspCI DraIII EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I FalI HaeII Hin4I HincII
KpnI MmeI MscI MslI MspA1I NaeI NcoI NgoMIV
NspI PasI PflMI PfoI PmlI PpiI PpuMI PspOMI
PstI PvuII SacI SapI SexAI SfcI SmaI SmlI
SphI StyI TaqII TatI Tth111I XcmI XmaI XmnI
Enzymes that do not cut:
AatII AccI AclI AflIII AhdI AloI AscI AseI
AsiSI AvrII BaeI BbeI BbvCI BciVI BclI BfrBI
BlpI BmgBI BmtI BsaBI BsaHI BsiWI BsmBI BspHI
BsrGI BssHII BssSI BstBI BstZ17I ClaI DraI DrdI
EcoRI EcoRV FseI FspI FspAI HindIII HpaI KasI
MfeI MluI NarI NdeI NheI NotI NruI NsiI
PacI PciI PmeI PshAI PsiI PspXI PsrI PvuI
RsrII SacII SalI SanDI SbfI ScaI SfiI SfoI
SgfI SgrAI SnaBI SpeI SrfI SspI StuI SwaI
TstI XbaI XhoI ZraI