Restriction map for
pF1KSDA1750
(Linear) (Six-base) MAPSORT of: KIAA1750.seq Check: 716 from: 1 to: 1251
KIAA1750.orf
With 182 enzymes: SgfI *
October 24, 2006 15:17 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 649 1251
Size: 649 602
AlwNI CAG_nnn'CTG
Cuts at: 0 358 1251
Size: 358 893
ApaI G_GGCC'C
Cuts at: 0 273 584 1251
Size: 273 311 667
Fragments arranged by size:
667 311 273
AvaI C'yCGr_G
Cuts at: 0 68 130 217 272 315 744 1251
Size: 68 62 87 55 43 429 507
Fragments arranged by size:
507 429 87 68 62 55 43
BanII G_rGCy'C
Cuts at: 0 273 584 854 1161 1251
Size: 273 311 270 307 90
Fragments arranged by size:
311 307 273 270 90
BbsI GAAGACnn'nnnn_
Cuts at: 0 143 354 1251
Size: 143 211 897
Fragments arranged by size:
897 211 143
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 317 351 1251
Size: 317 34 900
Fragments arranged by size:
900 317 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 600 1251
Size: 600 651
Bme1580I G_kGCm'C
Cuts at: 0 273 584 1251
Size: 273 311 667
Fragments arranged by size:
667 311 273
BmtI G_CTAG'C
Cuts at: 0 787 1251
Size: 787 464
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 494 546 1251
Size: 494 52 705
Fragments arranged by size:
705 494 52
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 68 634 655 663 991 1251
Size: 68 566 21 8 328 260
Fragments arranged by size:
566 328 260 68 21 8
Bpu10I CC'TnA_GC
Cuts at: 0 1015 1251
Size: 1015 236
BsaHI Gr'CG_yC
Cuts at: 0 92 1251
Size: 92 1159
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1057 1087 1251
Size: 1057 30 164
Fragments arranged by size:
1057 164 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 199 238 1251
Size: 199 39 1013
Fragments arranged by size:
1013 199 39
BseYI C'CCAG_C
Cuts at: 0 541 585 779 1251
Size: 541 44 194 472
Fragments arranged by size:
541 472 194 44
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 178 643 1251
Size: 178 465 608
Fragments arranged by size:
608 465 178
BsiEI CG_ry'CG
Cuts at: 0 9 897 1251
Size: 9 888 354
Fragments arranged by size:
888 354 9
BsiHKAI G_wGCw'C
Cuts at: 0 854 926 1251
Size: 854 72 325
Fragments arranged by size:
854 325 72
Bsp1286I G_dGCh'C
Cuts at: 0 273 584 854 926 1161 1251
Size: 273 311 270 72 235 90
Fragments arranged by size:
311 273 270 235 90 72
BsrBI CCG'CTC
Cuts at: 0 5 1251
Size: 5 1246
BsrFI r'CCGG_y
Cuts at: 0 236 419 1251
Size: 236 183 832
Fragments arranged by size:
832 236 183
BssSI C'ACGA_G
Cuts at: 0 1160 1251
Size: 1160 91
BstXI CCAn_nnnn'nTGG
Cuts at: 0 986 1251
Size: 986 265
Bsu36I CC'TnA_GG
Cuts at: 0 479 1251
Size: 479 772
BtgI C'CryG_G
Cuts at: 0 288 299 383 464 1251
Size: 288 11 84 81 787
Fragments arranged by size:
787 288 84 81 11
EaeI y'GGCC_r
Cuts at: 0 6 302 347 954 1251
Size: 6 296 45 607 297
Fragments arranged by size:
607 297 296 45 6
EagI C'GGCC_G
Cuts at: 0 6 1251
Size: 6 1245
EarI CTCTTCn'nnn_
Cuts at: 0 554 842 1251
Size: 554 288 409
Fragments arranged by size:
554 409 288
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 64 670 1251
Size: 64 606 581
Fragments arranged by size:
606 581 64
EcoICRI GAG'CTC
Cuts at: 0 852 1251
Size: 852 399
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 68 634 649 655 663 991 1251
Size: 68 566 15 6 8 328 260
Fragments arranged by size:
566 328 260 68 15 8 6
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1018 1251
Size: 1018 233
EcoO109I rG'GnC_Cy
Cuts at: 0 444 475 580 581 662 1251
Size: 444 31 105 1 81 589
Fragments arranged by size:
589 444 105 81 31 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 906 1251
Size: 906 345
HaeII r_GCGC'y
Cuts at: 0 345 1251
Size: 345 906
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 559 591 994 1026 1251
Size: 559 32 403 32 225
Fragments arranged by size:
559 403 225 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 393 1251
Size: 393 858
MscI TGG'CCA
Cuts at: 0 956 1251
Size: 956 295
MspA1I CmG'CkG
Cuts at: 0 193 301 376 466 541 627 1251
Size: 193 108 75 90 75 86 624
Fragments arranged by size:
624 193 108 90 86 75 75
NaeI GCC'GGC
Cuts at: 0 238 1251
Size: 238 1013
NgoMIV G'CCGG_C
Cuts at: 0 236 1251
Size: 236 1015
NheI G'CTAG_C
Cuts at: 0 783 1251
Size: 783 468
PasI CC'CwG_GG
Cuts at: 0 994 1251
Size: 994 257
PfoI T'CCnGG_A
Cuts at: 0 679 1200 1251
Size: 679 521 51
Fragments arranged by size:
679 521 51
PspOMI G'GGCC_C
Cuts at: 0 269 580 1251
Size: 269 311 671
Fragments arranged by size:
671 311 269
PstI C_TGCA'G
Cuts at: 0 707 1251
Size: 707 544
PvuI CG_AT'CG
Cuts at: 0 897 1251
Size: 897 354
PvuII CAG'CTG
Cuts at: 0 541 627 1251
Size: 541 86 624
Fragments arranged by size:
624 541 86
SacI G_AGCT'C
Cuts at: 0 854 1251
Size: 854 397
SacII CC_GC'GG
Cuts at: 0 302 467 1251
Size: 302 165 784
Fragments arranged by size:
784 302 165
SapI GCTCTTCn'nnn_
Cuts at: 0 842 1251
Size: 842 409
SfcI C'TryA_G
Cuts at: 0 456 703 1251
Size: 456 247 548
Fragments arranged by size:
548 456 247
SmaI CCC'GGG
Cuts at: 0 317 746 1251
Size: 317 429 505
Fragments arranged by size:
505 429 317
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 157 929 1043 1251
Size: 157 772 114 208
Fragments arranged by size:
772 208 157 114
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 925 957 1251
Size: 925 32 294
Fragments arranged by size:
925 294 32
Tth111I GACn'n_nGTC
Cuts at: 0 358 1251
Size: 358 893
XmaI C'CCGG_G
Cuts at: 0 315 744 1251
Size: 315 429 507
Fragments arranged by size:
507 429 315
Enzymes that do cut:
AcuI AlwNI ApaI AvaI BanII BbsI BcgI BciVI
Bme1580I BmtI BplI BpmI Bpu10I BsaHI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BsrBI BsrFI BssSI
BstXI Bsu36I BtgI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I FalI HaeII Hin4I MmeI MscI
MspA1I NaeI NgoMIV NheI PasI PfoI PspOMI PstI
PvuI PvuII SacI SacII SapI SfcI SmaI TaqII
TstI Tth111I XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaLI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BanI BbeI BbvCI
BclI BfrBI BglI BglII BlpI BmgBI BmrI BpuEI
BsaI BsaAI BsaBI BsaWI BsiWI BsmI BsmBI BspEI
BspHI BspMI BsrDI BsrGI BssHII BstAPI BstBI BstEII
BstYI BstZ17I BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EcoRI EcoRV FseI FspI FspAI HincII HindIII
HpaI KasI KpnI MfeI MluI MslI NarI NcoI
NdeI NotI NruI NsiI NspI PacI PciI PflMI
PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PsrI
RsrII SalI SanDI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmlI SnaBI SpeI SphI SrfI SspI
StuI StyI SwaI TatI XbaI XcmI XhoI XmnI
ZraI