Restriction map for
pF1KSDA1753
(Linear) (Six-base) MAPSORT of: KIAA1753.seq Check: 1127 from: 1 to: 2430
KIAA1753.orf
With 182 enzymes: SgfI *
October 24, 2006 15:17 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 187 2115 2430
Size: 187 1928 315
Fragments arranged by size:
1928 315 187
AatII G_ACGT'C
Cuts at: 0 263 2430
Size: 263 2167
AccI GT'mk_AC
Cuts at: 0 263 479 2430
Size: 263 216 1951
Fragments arranged by size:
1951 263 216
Acc65I G'GTAC_C
Cuts at: 0 356 2430
Size: 356 2074
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 481 1342 2430
Size: 481 861 1088
Fragments arranged by size:
1088 861 481
AflII C'TTAA_G
Cuts at: 0 2308 2430
Size: 2308 122
AflIII A'CryG_T
Cuts at: 0 101 2430
Size: 101 2329
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 641 919 1675 1685 2126 2430
Size: 641 278 756 10 441 304
Fragments arranged by size:
756 641 441 304 278 10
AlwNI CAG_nnn'CTG
Cuts at: 0 69 1353 1862 1930 2184 2430
Size: 69 1284 509 68 254 246
Fragments arranged by size:
1284 509 254 246 69 68
ApaI G_GGCC'C
Cuts at: 0 13 20 458 2256 2415 2430
Size: 13 7 438 1798 159 15
Fragments arranged by size:
1798 438 159 15 13 7
ApoI r'AATT_y
Cuts at: 0 115 1290 2430
Size: 115 1175 1140
Fragments arranged by size:
1175 1140 115
AvaI C'yCGr_G
Cuts at: 0 13 82 871 926 1893 2096 2430
Size: 13 69 789 55 967 203 334
Fragments arranged by size:
967 789 334 203 69 55 13
AvrII C'CTAG_G
Cuts at: 0 1125 2430
Size: 1125 1305
BanI G'GyrC_C
Cuts at: 0 42 209 238 356 1059 1468 2077 2430
Size: 42 167 29 118 703 409 609 353
Fragments arranged by size:
703 609 409 353 167 118 42 29
BanII G_rGCy'C
Cuts at: 0 13 20 458 739 1072 1165 1334 1669
Size: 13 7 438 281 333 93 169 335
Cuts at: 1669 2177 2256 2369 2415 2430
Size: 508 79 113 46 15
Fragments arranged by size:
508 438 335 333 281 169 113 93
79 46 15 13 7
BbeI G_GCGC'C
Cuts at: 0 46 213 2081 2430
Size: 46 167 1868 349
Fragments arranged by size:
1868 349 167 46
BbsI GAAGACnn'nnnn_
Cuts at: 0 1191 1268 1606 2430
Size: 1191 77 338 824
Fragments arranged by size:
1191 824 338 77
BbvCI CC'TCA_GC
Cuts at: 0 598 993 1815 2375 2430
Size: 598 395 822 560 55
Fragments arranged by size:
822 598 560 395 55
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 20 54 1626 1660 2430
Size: 20 34 1572 34 770
Fragments arranged by size:
1572 770 34 34 20
BglI GCCn_nnn'nGGC
Cuts at: 0 305 508 615 675 689 1466 1939 2017
Size: 305 203 107 60 14 777 473 78
Cuts at: 2017 2071 2405 2430
Size: 54 334 25
Fragments arranged by size:
777 473 334 305 203 107 78 60
54 25 14
BglII A'GATC_T
Cuts at: 0 875 2430
Size: 875 1555
Bme1580I G_kGCm'C
Cuts at: 0 13 20 136 319 458 1104 1471 1625
Size: 13 7 116 183 139 646 367 154
Cuts at: 1625 1900 2256 2392 2415 2430
Size: 275 356 136 23 15
Fragments arranged by size:
646 367 356 275 183 154 139 136
116 23 15 13 7
BmrI ACTGGGnnnn_n'
Cuts at: 0 1456 2430
Size: 1456 974
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 420 2229 2430
Size: 420 1809 201
Fragments arranged by size:
1809 420 201
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1059 1207 1419 1615 2122 2137 2170 2221
Size: 1059 148 212 196 507 15 33 51
Cuts at: 2221 2224 2407 2430
Size: 3 183 23
Fragments arranged by size:
1059 507 212 196 183 148 51 33
23 15 3
Bpu10I CC'TnA_GC
Cuts at: 0 62 598 993 1815 2171 2375 2430
Size: 62 536 395 822 356 204 55
Fragments arranged by size:
822 536 395 356 204 62 55
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1395 2430
Size: 1395 1035
BsaI GGTCTCn'nnnn_
Cuts at: 0 794 2430
Size: 794 1636
BsaAI yAC'GTr
Cuts at: 0 104 954 2430
Size: 104 850 1476
Fragments arranged by size:
1476 850 104
BsaHI Gr'CG_yC
Cuts at: 0 43 210 260 827 2078 2430
Size: 43 167 50 567 1251 352
Fragments arranged by size:
1251 567 352 167 50 43
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 454 484 598 628 1043 1073 2430
Size: 454 30 114 30 415 30 1357
Fragments arranged by size:
1357 454 415 114 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 619 1096 1740 2008 2062 2158 2430
Size: 619 477 644 268 54 96 272
Fragments arranged by size:
644 619 477 272 268 96 54
BseYI C'CCAG_C
Cuts at: 0 562 636 1013 1377 2430
Size: 562 74 377 364 1053
Fragments arranged by size:
1053 562 377 364 74
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 252 310 402 418 2143 2430
Size: 252 58 92 16 1725 287
Fragments arranged by size:
1725 287 252 92 58 16
BsiEI CG_ry'CG
Cuts at: 0 53 2430
Size: 53 2377
BsiHKAI G_wGCw'C
Cuts at: 0 1669 2369 2430
Size: 1669 700 61
Fragments arranged by size:
1669 700 61
BsmI GAATG_Cn'
Cuts at: 0 1497 2430
Size: 1497 933
BsmBI CGTCTCn'nnnn_
Cuts at: 0 767 818 2430
Size: 767 51 1612
Fragments arranged by size:
1612 767 51
Bsp1286I G_dGCh'C
Cuts at: 0 13 20 136 319 458 739 1072 1104
Size: 13 7 116 183 139 281 333 32
Cuts at: 1104 1165 1334 1471 1625 1669 1900 2177 2256
Size: 61 169 137 154 44 231 277 79
Cuts at: 2256 2369 2392 2415 2430
Size: 113 23 23 15
Fragments arranged by size:
333 281 277 231 183 169 154 139
137 116 113 79 61 44 32 23
23 15 13 7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 187 344 989 2115 2430
Size: 187 157 645 1126 315
Fragments arranged by size:
1126 645 315 187 157
BsrBI CCG'CTC
Cuts at: 0 61 470 2061 2154 2430
Size: 61 409 1591 93 276
Fragments arranged by size:
1591 409 276 93 61
BsrFI r'CCGG_y
Cuts at: 0 215 725 2430
Size: 215 510 1705
Fragments arranged by size:
1705 510 215
BsrGI T'GTAC_A
Cuts at: 0 1031 2430
Size: 1031 1399
BssSI C'ACGA_G
Cuts at: 0 394 2430
Size: 394 2036
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1353 1761 2387 2430
Size: 1353 408 626 43
Fragments arranged by size:
1353 626 408 43
BstXI CCAn_nnnn'nTGG
Cuts at: 0 562 2430
Size: 562 1868
BstYI r'GATC_y
Cuts at: 0 593 875 1828 2430
Size: 593 282 953 602
Fragments arranged by size:
953 602 593 282
BtgI C'CryG_G
Cuts at: 0 450 503 530 673 2430
Size: 450 53 27 143 1757
Fragments arranged by size:
1757 450 143 53 27
BtsI GCAGTG_nn'
Cuts at: 0 136 435 1993 2339 2430
Size: 136 299 1558 346 91
Fragments arranged by size:
1558 346 299 136 91
DraIII CAC_nnn'GTG
Cuts at: 0 198 893 2430
Size: 198 695 1537
Fragments arranged by size:
1537 695 198
EaeI y'GGCC_r
Cuts at: 0 50 522 1586 2406 2430
Size: 50 472 1064 820 24
Fragments arranged by size:
1064 820 472 50 24
EagI C'GGCC_G
Cuts at: 0 50 2430
Size: 50 2380
EarI CTCTTCn'nnn_
Cuts at: 0 1425 2430
Size: 1425 1005
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 214 747 2059 2430
Size: 214 533 1312 371
Fragments arranged by size:
1312 533 371 214
EcoICRI GAG'CTC
Cuts at: 0 1667 2367 2430
Size: 1667 700 63
Fragments arranged by size:
1667 700 63
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 481 1059 1207 1342 1419 1615 2122 2137
Size: 481 578 148 135 77 196 507 15
Cuts at: 2137 2170 2221 2224 2407 2430
Size: 33 51 3 183 23
Fragments arranged by size:
578 507 481 196 183 148 135 77
51 33 23 15 3
EcoNI CCTnn'n_nnAGG
Cuts at: 0 601 2430
Size: 601 1829
EcoO109I rG'GnC_Cy
Cuts at: 0 9 10 455 931 1021 2167 2181 2430
Size: 9 1 445 476 90 1146 14 249
Fragments arranged by size:
1146 476 445 249 90 14 9 1
EcoRI G'AATT_C
Cuts at: 0 115 2430
Size: 115 2315
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1436 2430
Size: 1436 994
HaeII r_GCGC'y
Cuts at: 0 46 213 2081 2212 2430
Size: 46 167 1868 131 218
Fragments arranged by size:
1868 218 167 131 46
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 454 486 541 573 2430
Size: 454 32 55 32 1857
Fragments arranged by size:
1857 454 55 32 32
HindIII A'AGCT_T
Cuts at: 0 1884 2430
Size: 1884 546
KasI G'GCGC_C
Cuts at: 0 42 209 2077 2430
Size: 42 167 1868 353
Fragments arranged by size:
1868 353 167 42
KpnI G_GTAC'C
Cuts at: 0 360 2430
Size: 360 2070
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1772 2222 2430
Size: 1772 450 208
Fragments arranged by size:
1772 450 208
MscI TGG'CCA
Cuts at: 0 524 2430
Size: 524 1906
MslI CAynn'nnrTG
Cuts at: 0 788 2430
Size: 788 1642
MspA1I CmG'CkG
Cuts at: 0 207 675 920 997 2106 2430
Size: 207 468 245 77 1109 324
Fragments arranged by size:
1109 468 324 245 207 77
NarI GG'CG_CC
Cuts at: 0 43 210 2078 2430
Size: 43 167 1868 352
Fragments arranged by size:
1868 352 167 43
NcoI C'CATG_G
Cuts at: 0 503 2430
Size: 503 1927
NspI r_CATG'y
Cuts at: 0 906 1038 1087 1377 2292 2430
Size: 906 132 49 290 915 138
Fragments arranged by size:
915 906 290 138 132 49
PasI CC'CwG_GG
Cuts at: 0 1204 1773 2430
Size: 1204 569 657
Fragments arranged by size:
1204 657 569
PfoI T'CCnGG_A
Cuts at: 0 1999 2138 2430
Size: 1999 139 292
Fragments arranged by size:
1999 292 139
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 847 879 2430
Size: 847 32 1551
Fragments arranged by size:
1551 847 32
PpuMI rG'GwC_Cy
Cuts at: 0 931 1021 2430
Size: 931 90 1409
Fragments arranged by size:
1409 931 90
PspOMI G'GGCC_C
Cuts at: 0 9 16 454 2252 2411 2430
Size: 9 7 438 1798 159 19
Fragments arranged by size:
1798 438 159 19 9 7
PstI C_TGCA'G
Cuts at: 0 74 986 1664 1766 2430
Size: 74 912 678 102 664
Fragments arranged by size:
912 678 664 102 74
PvuII CAG'CTG
Cuts at: 0 920 997 2430
Size: 920 77 1433
Fragments arranged by size:
1433 920 77
SacI G_AGCT'C
Cuts at: 0 1669 2369 2430
Size: 1669 700 61
Fragments arranged by size:
1669 700 61
SacII CC_GC'GG
Cuts at: 0 676 2430
Size: 676 1754
ScaI AGT'ACT
Cuts at: 0 838 2430
Size: 838 1592
SexAI A'CCwGG_T
Cuts at: 0 1265 2430
Size: 1265 1165
SfcI C'TryA_G
Cuts at: 0 70 287 982 1110 1660 1762 2430
Size: 70 217 695 128 550 102 668
Fragments arranged by size:
695 668 550 217 128 102 70
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 508 2430
Size: 508 1922
SfoI GGC'GCC
Cuts at: 0 44 211 2079 2430
Size: 44 167 1868 351
Fragments arranged by size:
1868 351 167 44
SmaI CCC'GGG
Cuts at: 0 15 1895 2098 2430
Size: 15 1880 203 332
Fragments arranged by size:
1880 332 203 15
SmlI C'TyrA_G
Cuts at: 0 1410 2308 2430
Size: 1410 898 122
Fragments arranged by size:
1410 898 122
SphI G_CATG'C
Cuts at: 0 1087 2430
Size: 1087 1343
StuI AGG'CCT
Cuts at: 0 293 511 2430
Size: 293 218 1919
Fragments arranged by size:
1919 293 218
StyI C'CwwG_G
Cuts at: 0 503 687 1125 1694 2430
Size: 503 184 438 569 736
Fragments arranged by size:
736 569 503 438 184
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1027 1643 2430
Size: 1027 616 787
Fragments arranged by size:
1027 787 616
TatI w'GTAC_w
Cuts at: 0 836 1031 2430
Size: 836 195 1399
Fragments arranged by size:
1399 836 195
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 598 630 1043 1075 1708 1740 2430
Size: 598 32 413 32 633 32 690
Fragments arranged by size:
690 633 598 413 32 32 32
XmaI C'CCGG_G
Cuts at: 0 13 1893 2096 2430
Size: 13 1880 203 334
Fragments arranged by size:
1880 334 203 13
XmnI GAAnn'nnTTC
Cuts at: 0 115 1291 1603 2430
Size: 115 1176 312 827
Fragments arranged by size:
1176 827 312 115
ZraI GAC'GTC
Cuts at: 0 261 2430
Size: 261 2169
Enzymes that do cut:
AarI AatII AccI Acc65I AcuI AflII AflIII AlfI
AlwNI ApaI ApoI AvaI AvrII BanI BanII BbeI
BbsI BbvCI BcgI BglI BglII Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspMI
BsrBI BsrFI BsrGI BssSI BstAPI BstXI BstYI BtgI
BtsI DraIII EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI HaeII Hin4I HindIII KasI
KpnI MmeI MscI MslI MspA1I NarI NcoI NspI
PasI PfoI PpiI PpuMI PspOMI PstI PvuII SacI
SacII ScaI SexAI SfcI SfiI SfoI SmaI SmlI
SphI StuI StyI TaqII TatI TstI XmaI XmnI
ZraI
Enzymes that do not cut:
AclI AfeI AgeI AhdI AleI AloI ApaLI AscI
AseI AsiSI BaeI BamHI BciVI BclI BfrBI BlpI
BmgBI BmtI BsaBI BsaWI BsiWI BspEI BspHI BsrDI
BssHII BstBI BstEII BstZ17I Bsu36I BtgZI ClaI CspCI
DraI DrdI EcoRV FseI FspI FspAI HincII HpaI
MfeI MluI NaeI NdeI NgoMIV NheI NotI NruI
NsiI PacI PciI PflMI PmeI PmlI PshAI PsiI
PspXI PsrI PvuI RsrII SalI SanDI SapI SbfI
SgfI SgrAI SnaBI SpeI SrfI SspI SwaI Tth111I
XbaI XcmI XhoI