Restriction map for
pF1KSDA1759
(Linear) (Six-base) MAPSORT of: KIAA1759.seq Check: 8444 from: 1 to: 2916
KIAA1759.orf
With 182 enzymes: SgfI *
October 24, 2006 15:17 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1895 2382 2916
Size: 1895 487 534
Fragments arranged by size:
1895 534 487
AccI GT'mk_AC
Cuts at: 0 54 2774 2916
Size: 54 2720 142
Fragments arranged by size:
2720 142 54
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1200 2629 2849 2916
Size: 1200 1429 220 67
Fragments arranged by size:
1429 1200 220 67
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1807 2191 2916
Size: 1807 384 725
Fragments arranged by size:
1807 725 384
AlwNI CAG_nnn'CTG
Cuts at: 0 178 277 432 1090 1530 2328 2397 2530
Size: 178 99 155 658 440 798 69 133
Cuts at: 2530 2916
Size: 386
Fragments arranged by size:
798 658 440 386 178 155 133 99
69
ApaI G_GGCC'C
Cuts at: 0 43 284 1879 2518 2622 2733 2906 2916
Size: 43 241 1595 639 104 111 173 10
Fragments arranged by size:
1595 639 241 173 111 104 43 10
ApoI r'AATT_y
Cuts at: 0 2797 2916
Size: 2797 119
AvaI C'yCGr_G
Cuts at: 0 35 97 167 580 2245 2720 2916
Size: 35 62 70 413 1665 475 196
Fragments arranged by size:
1665 475 413 196 70 62 35
BanI G'GyrC_C
Cuts at: 0 47 670 993 1385 1953 2916
Size: 47 623 323 392 568 963
Fragments arranged by size:
963 623 568 392 323 47
BanII G_rGCy'C
Cuts at: 0 43 284 733 794 1754 1879 2033 2150
Size: 43 241 449 61 960 125 154 117
Cuts at: 2150 2404 2518 2622 2689 2733 2906 2916
Size: 254 114 104 67 44 173 10
Fragments arranged by size:
960 449 254 241 173 154 125 117
114 104 67 61 44 43 10
BbsI GAAGACnn'nnnn_
Cuts at: 0 108 619 872 1649 2355 2916
Size: 108 511 253 777 706 561
Fragments arranged by size:
777 706 561 511 253 108
BbvCI CC'TCA_GC
Cuts at: 0 1234 2164 2510 2916
Size: 1234 930 346 406
Fragments arranged by size:
1234 930 406 346
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 148 181 182 215 242 276 2916
Size: 148 33 1 33 27 34 2640
Fragments arranged by size:
2640 148 34 33 33 27 1
BglI GCCn_nnn'nGGC
Cuts at: 0 1426 1535 2308 2626 2916
Size: 1426 109 773 318 290
Fragments arranged by size:
1426 773 318 290 109
BlpI GC'TnA_GC
Cuts at: 0 19 383 1300 2916
Size: 19 364 917 1616
Fragments arranged by size:
1616 917 364 19
Bme1580I G_kGCm'C
Cuts at: 0 43 50 284 500 673 801 1431 1879
Size: 43 7 234 216 173 128 630 448
Cuts at: 1879 2518 2622 2733 2906 2916
Size: 639 104 111 173 10
Fragments arranged by size:
639 630 448 234 216 173 173 128
111 104 43 10 7
BmgBI CAC'GTC
Cuts at: 0 1675 2916
Size: 1675 1241
BmrI ACTGGGnnnn_n'
Cuts at: 0 76 494 1748 2366 2514 2916
Size: 76 418 1254 618 148 402
Fragments arranged by size:
1254 618 418 402 148 76
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 437 1437 1758 2007 2916
Size: 437 1000 321 249 909
Fragments arranged by size:
1000 909 437 321 249
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 172 310 632 931 1532 1578 1652 1675
Size: 172 138 322 299 601 46 74 23
Cuts at: 1675 2690 2916
Size: 1015 226
Fragments arranged by size:
1015 601 322 299 226 172 138 74
46 23
Bpu10I CC'TnA_GC
Cuts at: 0 605 1234 1748 2144 2164 2510 2916
Size: 605 629 514 396 20 346 406
Fragments arranged by size:
629 605 514 406 396 346 20
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 44 2125 2788 2916
Size: 44 2081 663 128
Fragments arranged by size:
2081 663 128 44
BsaI GGTCTCn'nnnn_
Cuts at: 0 94 1206 2916
Size: 94 1112 1710
Fragments arranged by size:
1710 1112 94
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1048 1449 1639 1813 1843 1860 2122 2125
Size: 1048 401 190 174 30 17 262 3
Cuts at: 2125 2128 2916
Size: 3 788
Fragments arranged by size:
1048 788 401 262 190 174 30 17
3 3
BseYI C'CCAG_C
Cuts at: 0 507 724 873 1396 1554 1839 1858 1898
Size: 507 217 149 523 158 285 19 40
Cuts at: 1898 1969 2303 2321 2397 2451 2916
Size: 71 334 18 76 54 465
Fragments arranged by size:
523 507 465 334 285 217 158 149
76 71 54 40 19 18
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 206 272 286 973 1886 2801 2916
Size: 206 66 14 687 913 915 115
Fragments arranged by size:
915 913 687 206 115 66 14
BsiHKAI G_wGCw'C
Cuts at: 0 2033 2234 2916
Size: 2033 201 682
Fragments arranged by size:
2033 682 201
BsmI GAATG_Cn'
Cuts at: 0 2772 2916
Size: 2772 144
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2651 2916
Size: 2651 265
Bsp1286I G_dGCh'C
Cuts at: 0 43 50 284 500 673 733 794 801
Size: 43 7 234 216 173 60 61 7
Cuts at: 801 1431 1754 1879 2033 2150 2234 2404 2518
Size: 630 323 125 154 117 84 170 114
Cuts at: 2518 2622 2689 2733 2906 2916
Size: 104 67 44 173 10
Fragments arranged by size:
630 323 234 216 173 173 170 154
125 117 114 104 84 67 61 60
44 43 10 7 7
BspHI T'CATG_A
Cuts at: 0 899 2916
Size: 899 2017
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 112 1895 2382 2538 2916
Size: 112 1783 487 156 378
Fragments arranged by size:
1783 487 378 156 112
BsrBI CCG'CTC
Cuts at: 0 156 619 1737 2336 2916
Size: 156 463 1118 599 580
Fragments arranged by size:
1118 599 580 463 156
BsrDI GCAATG_nn'
Cuts at: 0 349 574 927 2064 2483 2916
Size: 349 225 353 1137 419 433
Fragments arranged by size:
1137 433 419 353 349 225
BsrFI r'CCGG_y
Cuts at: 0 1130 1311 2916
Size: 1130 181 1605
Fragments arranged by size:
1605 1130 181
BssSI C'ACGA_G
Cuts at: 0 304 1430 2916
Size: 304 1126 1486
Fragments arranged by size:
1486 1126 304
BstAPI GCAn_nnn'nTGC
Cuts at: 0 855 2530 2916
Size: 855 1675 386
Fragments arranged by size:
1675 855 386
BstEII G'GTnAC_C
Cuts at: 0 1882 2916
Size: 1882 1034
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1906 1922 2916
Size: 1906 16 994
Fragments arranged by size:
1906 994 16
BstYI r'GATC_y
Cuts at: 0 309 2875 2916
Size: 309 2566 41
Fragments arranged by size:
2566 309 41
BstZ17I GTA'TAC
Cuts at: 0 55 2916
Size: 55 2861
Bsu36I CC'TnA_GG
Cuts at: 0 1796 1928 2916
Size: 1796 132 988
Fragments arranged by size:
1796 988 132
BtgI C'CryG_G
Cuts at: 0 2490 2916
Size: 2490 426
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 735 2916
Size: 735 2181
BtsI GCAGTG_nn'
Cuts at: 0 232 811 2095 2455 2916
Size: 232 579 1284 360 461
Fragments arranged by size:
1284 579 461 360 232
DraIII CAC_nnn'GTG
Cuts at: 0 1678 2916
Size: 1678 1238
DrdI GACnn_nn'nnGTC
Cuts at: 0 1693 2916
Size: 1693 1223
EaeI y'GGCC_r
Cuts at: 0 486 782 1602 2916
Size: 486 296 820 1314
Fragments arranged by size:
1314 820 486 296
EarI CTCTTCn'nnn_
Cuts at: 0 901 1219 1467 1516 1829 2554 2759 2916
Size: 901 318 248 49 313 725 205 157
Fragments arranged by size:
901 725 318 313 248 205 157 49
EcoICRI GAG'CTC
Cuts at: 0 2031 2916
Size: 2031 885
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 172 310 632 931 1200 1532 1578 1652
Size: 172 138 322 299 269 332 46 74
Cuts at: 1652 1675 2629 2690 2849 2916
Size: 23 954 61 159 67
Fragments arranged by size:
954 332 322 299 269 172 159 138
74 67 61 46 23
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2107 2389 2522 2916
Size: 2107 282 133 394
Fragments arranged by size:
2107 394 282 133
EcoO109I rG'GnC_Cy
Cuts at: 0 39 40 163 274 281 1006 1220 1435
Size: 39 1 123 111 7 725 214 215
Cuts at: 1435 1875 1876 2514 2515 2527 2618 2729 2730
Size: 440 1 638 1 12 91 111 1
Cuts at: 2730 2903 2916
Size: 173 13
Fragments arranged by size:
725 638 440 215 214 173 123 111
111 91 39 13 12 7 1 1
1 1
FspI TGC'GCA
Cuts at: 0 208 2916
Size: 208 2708
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 2860 2892 2916
Size: 2860 32 24
Fragments arranged by size:
2860 32 24
HincII GTy'rAC
Cuts at: 0 230 329 2277 2916
Size: 230 99 1948 639
Fragments arranged by size:
1948 639 230 99
MfeI C'AATT_G
Cuts at: 0 2635 2916
Size: 2635 281
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2480 2635 2837 2916
Size: 2480 155 202 79
Fragments arranged by size:
2480 202 155 79
MscI TGG'CCA
Cuts at: 0 784 2916
Size: 784 2132
MslI CAynn'nnrTG
Cuts at: 0 1133 2227 2356 2916
Size: 1133 1094 129 560
Fragments arranged by size:
1133 1094 560 129
MspA1I CmG'CkG
Cuts at: 0 339 546 724 1238 1260 1348 1691 2193
Size: 339 207 178 514 22 88 343 502
Cuts at: 2193 2307 2916
Size: 114 609
Fragments arranged by size:
609 514 502 343 339 207 178 114
88 22
NaeI GCC'GGC
Cuts at: 0 1313 2916
Size: 1313 1603
NgoMIV G'CCGG_C
Cuts at: 0 1311 2916
Size: 1311 1605
NspI r_CATG'y
Cuts at: 0 1420 2256 2602 2916
Size: 1420 836 346 314
Fragments arranged by size:
1420 836 346 314
PasI CC'CwG_GG
Cuts at: 0 43 277 675 2692 2916
Size: 43 234 398 2017 224
Fragments arranged by size:
2017 398 234 224 43
PfoI T'CCnGG_A
Cuts at: 0 149 1437 1768 2033 2916
Size: 149 1288 331 265 883
Fragments arranged by size:
1288 883 331 265 149
PpuMI rG'GwC_Cy
Cuts at: 0 1006 1220 1435 2527 2916
Size: 1006 214 215 1092 389
Fragments arranged by size:
1092 1006 389 215 214
PspOMI G'GGCC_C
Cuts at: 0 39 280 1875 2514 2618 2729 2902 2916
Size: 39 241 1595 639 104 111 173 14
Fragments arranged by size:
1595 639 241 173 111 104 39 14
PspXI vC'TCGA_Gb
Cuts at: 0 580 2916
Size: 580 2336
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1055 1087 2916
Size: 1055 32 1829
Fragments arranged by size:
1829 1055 32
PstI C_TGCA'G
Cuts at: 0 596 1259 1623 2261 2393 2523 2916
Size: 596 663 364 638 132 130 393
Fragments arranged by size:
663 638 596 393 364 132 130
PvuII CAG'CTG
Cuts at: 0 339 546 724 1238 1260 1348 2193 2307
Size: 339 207 178 514 22 88 845 114
Cuts at: 2307 2916
Size: 609
Fragments arranged by size:
845 609 514 339 207 178 114 88
22
SacI G_AGCT'C
Cuts at: 0 2033 2916
Size: 2033 883
SapI GCTCTTCn'nnn_
Cuts at: 0 1829 2554 2759 2916
Size: 1829 725 205 157
Fragments arranged by size:
1829 725 205 157
SexAI A'CCwGG_T
Cuts at: 0 1711 2916
Size: 1711 1205
SfcI C'TryA_G
Cuts at: 0 592 1255 1619 2257 2389 2519 2916
Size: 592 663 364 638 132 130 397
Fragments arranged by size:
663 638 592 397 364 132 130
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 2308 2916
Size: 2308 608
SmaI CCC'GGG
Cuts at: 0 37 2722 2916
Size: 37 2685 194
Fragments arranged by size:
2685 194 37
SmlI C'TyrA_G
Cuts at: 0 59 580 2104 2245 2803 2916
Size: 59 521 1524 141 558 113
Fragments arranged by size:
1524 558 521 141 113 59
SphI G_CATG'C
Cuts at: 0 1420 2602 2916
Size: 1420 1182 314
Fragments arranged by size:
1420 1182 314
StuI AGG'CCT
Cuts at: 0 2119 2916
Size: 2119 797
StyI C'CwwG_G
Cuts at: 0 2576 2742 2916
Size: 2576 166 174
Fragments arranged by size:
2576 174 166
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1156 1522 2916
Size: 1156 366 1394
Fragments arranged by size:
1394 1156 366
TatI w'GTAC_w
Cuts at: 0 803 2916
Size: 803 2113
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2624 2656 2916
Size: 2624 32 260
Fragments arranged by size:
2624 260 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 842 2209 2916
Size: 842 1367 707
Fragments arranged by size:
1367 842 707
XhoI C'TCGA_G
Cuts at: 0 580 2245 2916
Size: 580 1665 671
Fragments arranged by size:
1665 671 580
XmaI C'CCGG_G
Cuts at: 0 35 2720 2916
Size: 35 2685 196
Fragments arranged by size:
2685 196 35
Enzymes that do cut:
AarI AccI AcuI AlfI AlwNI ApaI ApoI AvaI
BanI BanII BbsI BbvCI BcgI BglI BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BseRI
BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstEII BstXI BstYI
BstZ17I Bsu36I BtgI BtgZI BtsI DraIII DrdI EaeI
EarI EcoICRI Eco57MI EcoNI EcoO109I FspI Hin4I HincII
MfeI MmeI MscI MslI MspA1I NaeI NgoMIV NspI
PasI PfoI PpuMI PspOMI PspXI PsrI PstI PvuII
SacI SapI SexAI SfcI SfiI SmaI SmlI SphI
StuI StyI TaqII TatI TstI XcmI XhoI XmaI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI
AleI AloI ApaLI AscI AseI AsiSI AvrII BaeI
BamHI BbeI BciVI BclI BfrBI BglII BmtI BsaAI
BsaBI BsaHI BsaWI BsaXI BsiEI BsiWI BspEI BsrGI
BssHII BstBI ClaI CspCI DraI EagI EciI EcoRI
EcoRV FalI FseI FspAI HaeII HindIII HpaI KasI
KpnI MluI NarI NcoI NdeI NheI NotI NruI
NsiI PacI PciI PflMI PmeI PmlI PpiI PshAI
PsiI PvuI RsrII SacII SalI SanDI SbfI ScaI
SfoI SgfI SgrAI SnaBI SpeI SrfI SspI SwaI
Tth111I XbaI XmnI ZraI